pycom15g04250

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012254
Physical Location & Seq
Forward (+)
2560085 .. 2562934
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGAGGACGGTCGTGTCGCGGTTGATCCAACCAAGATCATCACTTCCAAGGCTCTTTGTATCGGATGCACTGAAGAATGGTAGGTATCTAACAACAGACTCTAATAAGGTTGATGAACCCTTCAAAGTTGAGGAAGCTGAGACTGTCAACATTCCCCCTCCTCCAACTGATAAGTTGCTTGTCTTGGGTGGAAATGGATTTGTTGGCTCACATGTTTGTAGAGAAGCATTGGGTCGGGGCTTGTCTGTCGCTAGCCTTAGCAGATCTGGTAGGTCAAAATTACATGATTCGTGGGCCAACACTGTGACCTGGCATCAAGGAAACCTTCTATCGCTTGAATCATTAAAGGATGCTTTCAATGGTGTCACGTCCGTTATATCCTGTGTTGGTGGGTTTGGCTCCAATTCTTACATGTATAAGATTAATGGGACTGCCAACATCAATGCAATTAGAGTTGCCGCTGAACAAGGCGTGAAAAGATTTGTGTATATCTCTGCTGCTGATTTTGGTGTGGCAAATTACTTACTGCAGGGATATTATGAGGGAAAGAGAGCTGCAGAGACAGAGCTACTAACCAAATTCCCATATGGAGGAGTGATTTTGAGGCCTGGATTTATATATGGGACTCGAACTGTTGGGAGTGTGAAGATACCTCTTGGTGTAATTGGTTCTCCTCTTGAGATGCTATTTCAAAACACGAGACCCCTGAGCCAGCTGCCACTTGTTGGGCCTCTCTTTACTCCTCCAGTGAATGTAACCTCTGTGGCAAATGTTGCCGTTAGAGCAGCTACTGATCCTGTCTTCCCTCCTGGGATTGTAGATGTCCCGGGTCTCCAGCGTTATACCCAGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

286

Amino Acids

30.74

Weight (kDa)

9.67

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 56 - 165 3.9e-10 NmrA-like family
Epimerase PF01370 60 - 167 5.3e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 60 - 187 1.5e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 63 - 208 4.6e-18 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 725
AccII CGCG 1 cut(s) 19
AciI CCGC 2 cut(s) 19, 459
AclWI GGATC 2 cut(s) 19, 788
AcsI RAATTY 1 cut(s) 578
AcuI CTGAAG 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 590, 725
AflIII ACRYGT 2 cut(s) 211, 411
AgsI TTSAA 4 cut(s) 124, 338, 358, 692
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 3 cut(s) 308, 607, 808
AluBI AGCT 5 cut(s) 137, 554, 568, 715, 788
AluI AGCT 5 cut(s) 137, 554, 568, 715, 788
Alw26I GTCTC 4 cut(s) 134, 554, 694, 836
AlwI GGATC 2 cut(s) 19, 788
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 3 cut(s) 294, 605, 728
ApeKI GCWGC 4 cut(s) 497, 554, 715, 785
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 294, 728
AsuC2I CCSGG 2 cut(s) 827, 828
AsuNHI GCTAGC 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 826
BauI CACGAG 1 cut(s) 697
BbsI GAAGAC 1 cut(s) 793
BbvI GCAGC 4 cut(s) 484, 541, 702, 797
BceAI ACGGC 1 cut(s) 761
BciT130I CCWGG 3 cut(s) 310, 609, 810
BcnI CCSGG 2 cut(s) 827, 828
BcoDI GTCTC 4 cut(s) 134, 554, 694, 836
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 527, 555
BglII AGATCT 1 cut(s) 263
BisI GCNGC 5 cut(s) 459, 498, 555, 716, 786
BlsI GCNGC 5 cut(s) 460, 499, 556, 717, 787
Bme1390I CCNGG 5 cut(s) 310, 609, 810, 827, 828
BmeT110I CYCGRG 1 cut(s) 826
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 294, 728
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 5 cut(s) 310, 609, 810, 827, 828
BmsI GCATC 4 cut(s) 55, 322, 340, 672
BmtI GCTAGC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 2 cut(s) 729, 818
Bpu10I CCTNAGC 2 cut(s) 257, 707
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 2 cut(s) 827, 828
BsaI GGTCTC 2 cut(s) 694, 836
BsaJI CCNNGG 3 cut(s) 47, 809, 826
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 725
Bse1I ACTGG 1 cut(s) 746
BseBI CCWGG 3 cut(s) 310, 609, 810
BseDI CCNNGG 3 cut(s) 47, 809, 826
BseGI GGATG 2 cut(s) 70, 355
BseLI CCNNNNNNNGG 2 cut(s) 590, 725
BseMII CTCAG 2 cut(s) 129, 698
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 4 cut(s) 150, 606, 663, 732
BseXI GCAGC 4 cut(s) 484, 541, 702, 797
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 296, 607, 730
BsiEI CGRYCG 1 cut(s) 12
BsiHKCI CYCGRG 1 cut(s) 826
BsiSI CCGG 1 cut(s) 827
BslFI GGGAC 3 cut(s) 442, 637, 809
BslI CCNNNNNNNGG 2 cut(s) 590, 725
BsmAI GTCTC 4 cut(s) 134, 554, 694, 836
BsmFI GGGAC 3 cut(s) 442, 637, 809
BsnI GGCC 3 cut(s) 296, 607, 730
Bso31I GGTCTC 2 cut(s) 694, 836
BsoBI CYCGRG 1 cut(s) 826
Bsp143I GATC 4 cut(s) 24, 35, 263, 793
BspACI CCGC 2 cut(s) 19, 459
BspANI GGCC 3 cut(s) 296, 607, 730
BspCNI CTCAG 2 cut(s) 130, 699
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 400
BspMAI CTGCAG 2 cut(s) 531, 559
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 19, 788
BspTNI GGTCTC 2 cut(s) 694, 836
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 47, 809, 826
BssMI GATC 4 cut(s) 24, 35, 263, 793
BssSI CACGAG 1 cut(s) 697
BssT1I CCWWGG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 697
Bst2UI CCWGG 3 cut(s) 310, 609, 810
Bst4CI ACNGT 4 cut(s) 10, 145, 304, 634
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 253, 713
BstDEI CTNAG 3 cut(s) 138, 257, 707
BstF5I GGATG 2 cut(s) 70, 355
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 4 cut(s) 27, 38, 266, 796
BstMAI GTCTC 4 cut(s) 134, 554, 694, 836
BstMBI GATC 4 cut(s) 24, 35, 263, 793
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 773, 782
BstNI CCWGG 3 cut(s) 310, 609, 810
BstNSI RCATGY 2 cut(s) 215, 415
BstSCI CCNGG 5 cut(s) 308, 607, 808, 825, 826
BstSFI CTRYAG 2 cut(s) 527, 555
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 4 cut(s) 484, 541, 702, 797
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 296, 607, 730
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 2 cut(s) 70, 355
BtsIMutI CAGTG 3 cut(s) 68, 300, 753
Cac8I GCNNGC 2 cut(s) 253, 713
CaiI CAGNNNCTG 1 cut(s) 791
Cfr13I GGNCC 2 cut(s) 294, 728
Cfr9I CCCGGG 1 cut(s) 826
CviAII CATG 3 cut(s) 212, 284, 412
DdeI CTNAG 3 cut(s) 138, 257, 707
DpnI GATC 4 cut(s) 26, 37, 265, 795
DpnII GATC 4 cut(s) 24, 35, 263, 793
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 47
Eco147I AGGCCT 1 cut(s) 607
Eco31I GGTCTC 2 cut(s) 694, 836
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 826
EcoRII CCWGG 3 cut(s) 308, 607, 808
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 3 cut(s) 215, 287, 415
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 442, 637, 809
FatI CATG 3 cut(s) 211, 283, 411
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 5 cut(s) 459, 498, 555, 716, 786
FokI GGATG 2 cut(s) 77, 362
Fsp4HI GCNGC 5 cut(s) 459, 498, 555, 716, 786
FspBI CTAG 1 cut(s) 252
GluI GCNGC 5 cut(s) 459, 498, 555, 716, 786
GsuI CTGGAG 2 cut(s) 729, 818
HaeIII GGCC 3 cut(s) 296, 607, 730
HapII CCGG 1 cut(s) 827
Hin1II CATG 3 cut(s) 215, 287, 415
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 4 cut(s) 98, 287, 338, 625
HpaII CCGG 1 cut(s) 827
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 677
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 2 cut(s) 130, 335
HpyCH4III ACNGT 4 cut(s) 10, 145, 304, 634
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 4 cut(s) 68, 446, 529, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 782
HpyF3I CTNAG 3 cut(s) 138, 257, 707
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 215, 287, 415
Kzo9I GATC 4 cut(s) 24, 35, 263, 793
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 4 cut(s) 484, 541, 702, 797
LweI GCATC 4 cut(s) 55, 322, 340, 672
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 3 cut(s) 304, 364, 754
MalI GATC 4 cut(s) 26, 37, 265, 795
MboI GATC 4 cut(s) 24, 35, 263, 793
MboII GAAGA 3 cut(s) 85, 658, 793
MflI RGATCY 1 cut(s) 263
MluCI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
MlyI GAGTC 2 cut(s) 92, 619
MmeI TCCRAC 2 cut(s) 52, 188
MseI TTAA 2 cut(s) 344, 423
MspA1I CMGCKG 2 cut(s) 461, 715
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 5 cut(s) 310, 609, 810, 827, 828
MvaI CCWGG 3 cut(s) 310, 609, 810
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 2 cut(s) 773, 782
NciI CCSGG 2 cut(s) 827, 828
NdeI CATATG 1 cut(s) 586
NdeII GATC 4 cut(s) 24, 35, 263, 793
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 215, 287, 415
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 304, 364
NspI RCATGY 2 cut(s) 215, 415
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 2 cut(s) 211, 411
PfeI GAWTC 2 cut(s) 287, 338
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 5 cut(s) 460, 499, 556, 717, 787
PleI GAGTC 2 cut(s) 92, 619
PpsI GAGTC 2 cut(s) 92, 619
PscI ACATGT 2 cut(s) 211, 411
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 308, 607, 808
PspGI CCWGG 3 cut(s) 308, 607, 808
PspN4I GGNNCC 1 cut(s) 400
PspPI GGNCC 2 cut(s) 294, 728
PstI CTGCAG 2 cut(s) 531, 559
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 715
SaqAI TTAA 2 cut(s) 344, 423
SatI GCNGC 5 cut(s) 459, 498, 555, 716, 786
Sau3AI GATC 4 cut(s) 24, 35, 263, 793
Sau96I GGNCC 2 cut(s) 294, 728
SchI GAGTC 2 cut(s) 92, 619
ScrFI CCNGG 5 cut(s) 310, 609, 810, 827, 828
SfaNI GCATC 4 cut(s) 55, 322, 340, 672
SfcI CTRYAG 2 cut(s) 527, 555
SmaI CCCGGG 1 cut(s) 828
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 6 cut(s) 278, 403, 447, 517, 578, 663
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 2 cut(s) 19, 459
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 5 cut(s) 308, 607, 808, 825, 826
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 4 cut(s) 10, 145, 304, 634
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 628
TasI AATT 6 cut(s) 278, 403, 447, 517, 578, 663
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 2 cut(s) 287, 338
Tru1I TTAA 2 cut(s) 344, 423
Tru9I TTAA 2 cut(s) 344, 423
TscAI CASTG 3 cut(s) 75, 307, 753
TseFI GTSAC 2 cut(s) 304, 364
TseI GCWGC 4 cut(s) 497, 554, 715, 785
Tsp45I GTSAC 2 cut(s) 304, 364
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 826
TspRI CASTG 3 cut(s) 75, 307, 753
Van91I CCANNNNNTGG 1 cut(s) 725
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 578
XceI RCATGY 2 cut(s) 215, 415
XmaI CCCGGG 1 cut(s) 826
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.