pycom15g04260

TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006093
Physical Location & Seq
Forward (+)
2563281 .. 2565387
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGCTTTTAAAATATCTTCTTGTTATAAATTTGTTGAAATTTGAGAGTGCTTCACCTTTAGCGGGTTTGCTTTTTCTAGAACGAGTGTTTTTACGTTGCATACACTTATCAAGTTTTCTTGCTCAACTTTACAGAAGCAGTGGCAAACATGCCCTAGAGAAGTGCTCATGCACAAAAGTGCTTTCTAGAAAAGTACTTTTAAACCGGCTTGGAACACATCCTACTGGAGTACTAGTAACCAAAACGGGCAATTGGATAAAAATAGGCCAATCAATGAACATCCTAGAGAAGACTGTTAACAGTCTGCACAGGCAGTCACAAAAGATCATATATTGGTATTGGAAAGAGAGAGAGGGAAAGAAGATGAACCAGAACCAGCAACCGAGCGACGGCGGAAAGCACGAGGATGACGCCGCCCTCACCGACTTCCTCGCCTCCCTGATGGACTACACCCCCACCATGCCCGACGAGCTCGTGGAGCATTACTTGGCGAAAAGCGGCTTCCAATGCCCCGACGTTCGATTAATCAGGCTGGTAGCTGTCGCCACACAAAAGTTTGTTTCTGAGGTCGCAACTGATGCTCTTCAGCAATGCAAAGCGAGGCAGGCTTCTATAGTCAAAGACAAAAGGGACAAACAACAAAAGGATAAGCGTCTAATATTGACAATGGAAGACCTGTCAAGAGCATTGCGTGAGTATGGCGTTAATGTGAAGCACCAAGAATATTTTGCGGACTGCCCTTCGAATGGGATGGATCCTGCAACACGGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000123 GO:0000125 GO:0000278 GO:0000428 GO:0001889 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005737 GO:0006139 GO:0006325 GO:0006351 GO:0006352 GO:0006355 GO:0006366 GO:0006367 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006725 GO:0006807 GO:0006915 GO:0006996 GO:0007049 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009059 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0012501 GO:0016043 GO:0016070 GO:0016569 GO:0016570 GO:0016573 GO:0016578 GO:0016579 GO:0016591 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019899 GO:0022402 GO:0022607 GO:0030154 GO:0030331 GO:0030855 GO:0030880 GO:0030914 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033276 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0035258 GO:0035264 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043933 GO:0043966 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044770 GO:0044772 GO:0044798 GO:0044843 GO:0045935 GO:0046483 GO:0048471 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051259 GO:0051260 GO:0051276 GO:0051427 GO:0055029 GO:0060255 GO:0060429 GO:0061008 GO:0061695 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070063 GO:0070365 GO:0070461 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902493 GO:1902494 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05168 map03022 map05168
Pfam Domains
Protein Families
TFIID_30kDa

Protein Analysis

259

Amino Acids

29.46

Weight (kDa)

9.21

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF10 PF03540 140 - 243 2.2e-38 Transcription initiation factor TFIID 23-30kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AciI CCGC 5 cut(s) 62, 393, 414, 498, 731
AclWI GGATC 2 cut(s) 749, 762
AcsI RAATTY 2 cut(s) 28, 38
AcuI CTGAAG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 2 cut(s) 195, 231
AfiI CCNNNNNNNGG 3 cut(s) 62, 389, 746
AgsI TTSAA 1 cut(s) 37
AhlI ACTAGT 1 cut(s) 232
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 2 cut(s) 472, 539
AluI AGCT 2 cut(s) 472, 539
Alw21I GWGCWC 2 cut(s) 167, 474
AlwI GGATC 2 cut(s) 749, 762
AoxI GGCC 1 cut(s) 265
ApoI RAATTY 2 cut(s) 28, 38
AseI ATTAAT 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 45, 412
AsuII TTCGAA 1 cut(s) 743
BamHI GGATCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 474
BauI CACGAG 2 cut(s) 401, 473
BbsI GAAGAC 2 cut(s) 296, 678
Bbv12I GWGCWC 2 cut(s) 167, 474
BccI CCATC 2 cut(s) 436, 745
BceAI ACGGC 1 cut(s) 406
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 5 cut(s) 77, 155, 186, 233, 284
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 2 cut(s) 414, 499
BlsI GCNGC 2 cut(s) 415, 500
BmcAI AGTACT 2 cut(s) 195, 231
BmiI GGNNCC 1 cut(s) 756
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 2 cut(s) 296, 678
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 389, 746
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 2 cut(s) 596, 686
BseGI GGATG 4 cut(s) 217, 279, 412, 756
BseLI CCNNNNNNNGG 3 cut(s) 62, 389, 746
BseMI GCAATG 2 cut(s) 596, 686
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 290
BshFI GGCC 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 167, 474
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 3 cut(s) 62, 389, 746
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 743
Bsp1286I GDGCHC 2 cut(s) 167, 474
Bsp143I GATC 2 cut(s) 324, 754
BspACI CCGC 5 cut(s) 62, 393, 414, 498, 731
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 756
BspPI GGATC 2 cut(s) 749, 762
BspQI GCTCTTC 1 cut(s) 588
BspT104I TTCGAA 1 cut(s) 743
BsrDI GCAATG 2 cut(s) 596, 686
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 204
BssMI GATC 2 cut(s) 324, 754
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 2 cut(s) 401, 473
Bst2BI CACGAG 2 cut(s) 401, 473
Bst4CI ACNGT 2 cut(s) 295, 302
Bst6I CTCTTC 2 cut(s) 588, 765
BstACI GRCGYC 1 cut(s) 411
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBI TTCGAA 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 1 cut(s) 564
BstF5I GGATG 4 cut(s) 217, 279, 412, 756
BstKTI GATC 2 cut(s) 327, 757
BstMBI GATC 2 cut(s) 324, 754
BstMWI GCNNNNNNNGC 5 cut(s) 469, 478, 507, 578, 605
BstNSI RCATGY 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 612
BstV2I GAAGAC 2 cut(s) 296, 678
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 4 cut(s) 217, 279, 412, 756
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 606
Cfr10I RCCGGY 1 cut(s) 204
CseI GACGC 2 cut(s) 419, 641
Csp6I GTAC 2 cut(s) 194, 230
CviAII CATG 3 cut(s) 149, 168, 460
CviJI RGCY 7 cut(s) 208, 267, 472, 501, 532, 539, 608
CviKI_1 RGCY 7 cut(s) 208, 267, 472, 501, 532, 539, 608
CviQI GTAC 2 cut(s) 194, 230
DdeI CTNAG 1 cut(s) 564
DpnI GATC 2 cut(s) 326, 756
DpnII GATC 2 cut(s) 324, 754
DraI TTTAAA 2 cut(s) 9, 201
Eam1104I CTCTTC 2 cut(s) 588, 765
EarI CTCTTC 2 cut(s) 588, 765
EciI GGCGGA 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 3 cut(s) 152, 171, 463
FaiI YATR 9 cut(s) 26, 101, 150, 169, 329, 331, 461, 614, 699
FaqI GGGAC 1 cut(s) 644
FatI CATG 3 cut(s) 148, 167, 459
FauI CCCGC 1 cut(s) 55
Fnu4HI GCNGC 2 cut(s) 414, 499
FokI GGATG 4 cut(s) 204, 266, 419, 763
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 2 cut(s) 414, 499
FspBI CTAG 5 cut(s) 77, 155, 186, 233, 284
GluI GCNGC 2 cut(s) 414, 499
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 267
HapII CCGG 1 cut(s) 205
HgaI GACGC 2 cut(s) 419, 641
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 3 cut(s) 152, 171, 463
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 45, 412
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 565
Hpy188III TCNNGA 3 cut(s) 77, 186, 681
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 3 cut(s) 392, 470, 518
HpyAV CCTTC 1 cut(s) 750
HpyCH4III ACNGT 2 cut(s) 295, 302
HpyCH4IV ACGT 2 cut(s) 94, 516
HpyCH4V TGCA 5 cut(s) 99, 171, 307, 594, 761
HpyF10VI GCNNNNNNNGC 5 cut(s) 469, 478, 507, 578, 605
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 2 cut(s) 94, 516
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 3 cut(s) 152, 171, 463
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 324, 754
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 1 cut(s) 478
LweI GCATC 1 cut(s) 568
MaeI CTAG 5 cut(s) 77, 155, 186, 233, 284
MaeII ACGT 2 cut(s) 94, 516
MaeIII GTNAC 2 cut(s) 235, 315
MalI GATC 2 cut(s) 326, 756
MboI GATC 2 cut(s) 324, 754
MboII GAAGA 5 cut(s) 8, 301, 373, 575, 683
MfeI CAATTG 1 cut(s) 250
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 2 cut(s) 167, 474
MluCI AATT 3 cut(s) 28, 38, 250
MnlI CCTC 7 cut(s) 346, 397, 428, 440, 445, 559, 594
MseI TTAA 5 cut(s) 8, 200, 297, 524, 705
MslI CAYNNNNRTG 2 cut(s) 176, 405
MspI CCGG 1 cut(s) 205
MunI CAATTG 1 cut(s) 250
MwoI GCNNNNNNNGC 5 cut(s) 469, 478, 507, 578, 605
NdeII GATC 2 cut(s) 324, 754
NlaIII CATG 3 cut(s) 152, 171, 463
NlaIV GGNNCC 1 cut(s) 756
NmuCI GTSAC 1 cut(s) 315
NspI RCATGY 1 cut(s) 152
NspV TTCGAA 1 cut(s) 743
OliI CACNNNNGTG 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 588
PkrI GCNGC 2 cut(s) 415, 500
PshBI ATTAAT 1 cut(s) 524
PsiI TTATAA 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 756
PsrI GAACNNNNNNTAC 2 cut(s) 205, 237
PsuI RGATCY 1 cut(s) 754
RsaI GTAC 2 cut(s) 195, 231
RsaNI GTAC 2 cut(s) 194, 230
RseI CAYNNNNRTG 2 cut(s) 176, 405
SacI GAGCTC 1 cut(s) 474
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 5 cut(s) 8, 200, 297, 524, 705
SatI GCNGC 2 cut(s) 414, 499
Sau3AI GATC 2 cut(s) 324, 754
ScaI AGTACT 2 cut(s) 195, 231
SduI GDGCHC 2 cut(s) 167, 474
SetI ASST 7 cut(s) 58, 97, 474, 519, 541, 570, 678
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 612
SfuI TTCGAA 1 cut(s) 743
SmiMI CAYNNNNRTG 2 cut(s) 176, 405
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 3 cut(s) 28, 38, 250
SsiI CCGC 5 cut(s) 62, 393, 414, 498, 731
SspI AATATT 2 cut(s) 660, 725
SspMI CTAG 5 cut(s) 77, 155, 186, 233, 284
SstI GAGCTC 1 cut(s) 474
TaaI ACNGT 2 cut(s) 295, 302
TaiI ACGT 2 cut(s) 97, 519
TaqI TCGA 2 cut(s) 520, 743
TasI AATT 3 cut(s) 28, 38, 250
TatI WGTACW 2 cut(s) 193, 229
TauI GCSGC 2 cut(s) 416, 501
Tru1I TTAA 5 cut(s) 8, 200, 297, 524, 705
Tru9I TTAA 5 cut(s) 8, 200, 297, 524, 705
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 315
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 2 cut(s) 290, 380
TspRI CASTG 1 cut(s) 145
VspI ATTAAT 1 cut(s) 524
XapI RAATTY 2 cut(s) 28, 38
XbaI TCTAGA 2 cut(s) 76, 185
XceI RCATGY 1 cut(s) 152
XspI CTAG 5 cut(s) 77, 155, 186, 233, 284
ZrmI AGTACT 2 cut(s) 195, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.