pycom15g05790

Vacuolar-processing enzyme-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009785
Physical Location & Seq
Forward (+)
3510250 .. 3513530
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1485 bp
ATGACTCGGTTAGCCGGCGCCGTCGTCCTCCTATTTCTCTCCTCCGTCTTGGCTTCCGCAGCCGGTAGCCGGGACCTAATTGGAGACGTTTTGCGATTACCGTCGGAAGCTTCCAGATTCTTCGGGAGAGGCGATGATGCTCCCGATCAGCAAGACGACGGCACCGTCGGGACCAGGTGGGCTGTTCTGATCGCCGGCTCCAATGGTTACTGGAATTACAGGCATCAGGCTGATATTTGTCACGCCTATCAACTACTGAAGAAAGGTGGGTTGAAAGATGAAAACATCGTTGTATTCATGTACGATGATATTGCTTACAACGAAGAGAACCCGAGGCAAGGAGTCATCATTAACAGCCCGCAGGGGAGTGACGTTTACGAAGGAGTTACAAAGGATTACACTGGGGAAGATGTTACCGTGAACAACTTTTTTGCCGCTATCCTTGGGAATAAAACTGCTCTGACCGGGGGTAGTGGGAAGGTTGTGGATAGCGGTCCAAATGATCATATTTTCATATACTACACCGATCATGGTGGTCCTGGAGTTCTTGGGATGCCGACTAGTCCTTATATTTATGCTAACGATCTGATAGAAGTCCTGAAGAAGAAGCATGCTGCCGGAACCTATAAAAGCTTAGTATTTTATCTGGAAGCTTGTGAATCTGGAAGTATCTTCGAGGGTCTTCTTCCGGAAGGTTTGAATATCTTTGCAACCACTGCGTCGAATGCAGAAGAGAGCAGTTGGGGAACTTACTGCCCTGGAGAGTATCCTAGTCCTCCCCCAGAATACGAGACCTGCTTGGGTGACTTGTATAGTGTTGCTTGGATGGAAGACAGTGACATACACAACTTGAGGTCAGAAACTTTGCACCAGCAATATGAACTGGTTAAAACGAGGACAGCCAATGACAATTATGGATTTGGTTCTCATGTCATGCAATACGGCGATGTAGGGCTCAGCAAGAACAACCTCTTTGTGTACATGGGGACAAATCCTGCAAATGACAACTACACTTTTCTGGGTGAGAACTCCTTGAGGCCATCTTCAAAAGCTGTCAACCAGCGCGATGCTGATCTTCTTCATTTCTGGCATAAGTATCGTAAGGCTCCAGAAGGCTCTGCAAGGAAAGTTCAAGCTCAAAAGGATTTTGTTGAAGCAATGTCTCACAGAATGCACATAGATCAGACCATGAAGCTCATTGGGAAACTCCTGTTCGGAATTGAAAAAGGCCCACAAGTACTCAACGCTGTTCGACCTGCTGGGCAGCCGCTTGTTGATGACTGGGACTGCCTTAAGACAATGGTGAGGAGTTTTGAGACACATTGTGGATCCCTATCTCAATACGGGATGAAACACATGCGTTCCCTGGCAAACATCTGCAACGTTGGAATGACACAGGAGCAGATGGCCGAGGCTTCAGCACAAGCCTGCGTGAGCGCCCCTTCTGGTTGCTGGAGCTCCCTGCACCGGGGATTCAGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04612 map04142 map04612
Pfam Domains
Protein Families
Peptidase_C13

Protein Analysis

495

Amino Acids

54.28

Weight (kDa)

5.39

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 59 - 330 3.1e-118 Peptidase C13 family
Legum_prodom PF20985 382 - 477 5e-43 Legumain, prodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25940 AT4G32940
fragaria_vesca FvH4_2g32190 FvH4_2g32190 FvH4_2g32190 FvH4_2g32190
malus_domestica MD08G1073500.v1.1
prunus_persica Prupe.1G415000_v2.0.a1 Prupe.1G415000_v2.0.a1
pyrus_communis pycom08g06090 pycom15g05790
rosa_chinensis RchiOBHm_Chr6g0310481
rosa_laevigata RLG00000010479
rosa_multiflora Rmu_sc0035020.1_g000006
rosa_roxburghii Rroxscaffold_7G00158490
rosa_rugosa Rorug06G0380700
rosa_samantha Rh6AG494700 Rh6BG504400 Rh6CG509600 Rh6DG495600
rosa_wichuraiana Rw6G043030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc36I ACCTGC 2 cut(s) 803, 1264
AccB1I GGYRCC 2 cut(s) 17, 161
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 5 cut(s) 57, 359, 435, 492, 1268
AclI AACGTT 1 cut(s) 1383
AclWI GGATC 2 cut(s) 1323, 1336
AcoI YGGCCR 1 cut(s) 1407
AcuI CTGAAG 3 cut(s) 278, 620, 1401
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 3 cut(s) 302, 980, 1239
AfiI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
AflII CTTAAG 1 cut(s) 1292
AgsI TTSAA 6 cut(s) 274, 700, 1047, 1133, 1154, 1223
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 4 cut(s) 173, 538, 757, 1365
AluBI AGCT 7 cut(s) 110, 633, 653, 1052, 1136, 1195, 1458
AluI AGCT 7 cut(s) 110, 633, 653, 1052, 1136, 1195, 1458
Alw21I GWGCWC 1 cut(s) 1460
Alw26I GTCTC 4 cut(s) 78, 785, 1167, 1310
AlwI GGATC 2 cut(s) 1323, 1336
Ama87I CYCGRG 1 cut(s) 331
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 3 cut(s) 1037, 1228, 1407
ApeKI GCWGC 3 cut(s) 59, 614, 1264
AspLEI GCGC 3 cut(s) 20, 1065, 1439
AspS9I GGNCC 5 cut(s) 73, 171, 494, 536, 1229
AsuC2I CCSGG 3 cut(s) 71, 466, 1469
AsuHPI GGTGA 3 cut(s) 815, 1034, 1315
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 4 cut(s) 73, 171, 494, 536
BamHI GGATCC 1 cut(s) 1328
BanI GGYRCC 2 cut(s) 17, 161
BanII GRGCYC 2 cut(s) 957, 1460
BbsI GAAGAC 2 cut(s) 674, 837
Bbv12I GWGCWC 1 cut(s) 1460
BbvI GCAGC 3 cut(s) 71, 601, 1276
BccI CCATC 3 cut(s) 820, 1048, 1399
BceAI ACGGC 3 cut(s) 5, 175, 958
BcgI CGANNNNNNTGC 4 cut(s) 293, 327, 1079, 1113
BciT130I CCWGG 4 cut(s) 175, 540, 759, 1367
BciVI GTATCC 1 cut(s) 777
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 3 cut(s) 71, 466, 1469
BcoDI GTCTC 4 cut(s) 78, 785, 1167, 1310
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 2 cut(s) 561, 771
BfoI RGCGCY 2 cut(s) 21, 1440
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 2 cut(s) 803, 1264
BfuI GTATCC 1 cut(s) 777
BisI GCNGC 5 cut(s) 60, 435, 615, 1265, 1268
BlpI GCTNAGC 1 cut(s) 956
BlsI GCNGC 5 cut(s) 61, 436, 616, 1266, 1269
BmcAI AGTACT 1 cut(s) 1239
Bme1390I CCNGG 7 cut(s) 71, 175, 466, 540, 759, 1367, 1469
Bme18I GGWCC 4 cut(s) 73, 171, 494, 536
BmeT110I CYCGRG 1 cut(s) 331
BmgT120I GGNCC 5 cut(s) 73, 171, 494, 536, 1229
BmiI GGNNCC 8 cut(s) 19, 74, 163, 172, 199, 622, 1107, 1330
BmrFI CCNGG 7 cut(s) 71, 175, 466, 540, 759, 1367, 1469
BmrI ACTGGG 2 cut(s) 411, 1291
BmsI GCATC 4 cut(s) 127, 232, 543, 1057
BmuI ACTGGG 2 cut(s) 411, 1291
BpiI GAAGAC 2 cut(s) 674, 837
BpmI CTGGAG 4 cut(s) 561, 780, 1092, 1474
Bpu1102I GCTNAGC 1 cut(s) 956
BpuEI CTTGAG 2 cut(s) 871, 1054
BpuMI CCSGG 3 cut(s) 71, 466, 1469
BsaBI GATNNNNATC 2 cut(s) 1071, 1333
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 785
BsaJI CCNNGG 7 cut(s) 332, 442, 465, 757, 1365, 1410, 1468
BsaWI WCCGGW 1 cut(s) 688
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
Bse118I RCCGGY 3 cut(s) 14, 62, 194
Bse1I ACTGG 4 cut(s) 215, 406, 888, 1286
Bse3DI GCAATG 1 cut(s) 1164
Bse8I GATNNNNATC 2 cut(s) 1071, 1333
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 4 cut(s) 175, 540, 759, 1367
BseDI CCNNGG 7 cut(s) 332, 442, 465, 757, 1365, 1410, 1468
BseGI GGATG 3 cut(s) 558, 831, 1353
BseJI GATNNNNATC 2 cut(s) 1071, 1333
BseLI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
BseMI GCAATG 1 cut(s) 1164
BseMII CTCAG 1 cut(s) 970
BseNI ACTGG 4 cut(s) 215, 406, 888, 1286
BseRI GAGGAG 2 cut(s) 31, 1321
BseXI GCAGC 3 cut(s) 71, 601, 1276
BseYI CCCAGC 1 cut(s) 1259
BsgI GTGCAG 1 cut(s) 1448
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 3 cut(s) 1039, 1230, 1409
BshNI GGYRCC 2 cut(s) 17, 161
BsiHKAI GWGCWC 1 cut(s) 1460
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 8 cut(s) 15, 63, 70, 195, 465, 618, 689, 1468
BslFI GGGAC 4 cut(s) 86, 184, 1000, 1298
BslI CCNNNNNNNGG 4 cut(s) 69, 338, 1467, 1468
BsmAI GTCTC 4 cut(s) 78, 785, 1167, 1310
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 4 cut(s) 86, 184, 1000, 1298
BsmI GAATGC 2 cut(s) 730, 1176
BsnI GGCC 3 cut(s) 1039, 1230, 1409
Bso31I GGTCTC 1 cut(s) 785
BsoBI CYCGRG 1 cut(s) 331
Bsp1286I GDGCHC 2 cut(s) 957, 1460
Bsp13I TCCGGA 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
Bsp1720I GCTNAGC 1 cut(s) 956
BspACI CCGC 5 cut(s) 57, 359, 435, 492, 1268
BspANI GGCC 3 cut(s) 1039, 1230, 1409
BspCNI CTCAG 1 cut(s) 969
BspEI TCCGGA 1 cut(s) 688
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 8 cut(s) 19, 74, 163, 172, 199, 622, 1107, 1330
BspMI ACCTGC 2 cut(s) 803, 1264
BspPI GGATC 2 cut(s) 1323, 1336
BspT107I GGYRCC 2 cut(s) 17, 161
BspTI CTTAAG 1 cut(s) 1292
BspTNI GGTCTC 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 1164
BsrFI RCCGGY 3 cut(s) 14, 62, 194
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 4 cut(s) 215, 406, 888, 1286
BssAI RCCGGY 3 cut(s) 14, 62, 194
BssECI CCNNGG 7 cut(s) 332, 442, 465, 757, 1365, 1410, 1468
BssMI GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 4 cut(s) 175, 540, 759, 1367
Bst4CI ACNGT 4 cut(s) 102, 166, 418, 836
Bst6I CTCTTC 2 cut(s) 318, 726
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 1 cut(s) 716
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 5 cut(s) 16, 196, 359, 612, 1429
BstDEI CTNAG 2 cut(s) 634, 956
BstF5I GGATG 3 cut(s) 558, 831, 1353
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 2 cut(s) 21, 1440
BstHHI GCGC 3 cut(s) 20, 1065, 1439
BstKTI GATC 8 cut(s) 148, 192, 505, 529, 586, 1075, 1183, 1331
BstMAI GTCTC 4 cut(s) 78, 785, 1167, 1310
BstMBI GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
BstMWI GCNNNNNNNGC 3 cut(s) 59, 716, 725
BstNI CCWGG 4 cut(s) 175, 540, 759, 1367
BstNSI RCATGY 2 cut(s) 614, 1360
BstSCI CCNGG 7 cut(s) 69, 173, 464, 538, 757, 1365, 1467
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 3 cut(s) 71, 601, 1276
BstV2I GAAGAC 2 cut(s) 674, 837
BstX2I RGATCY 1 cut(s) 1328
BstYI RGATCY 1 cut(s) 1328
BsuI GTATCC 1 cut(s) 777
BsuRI GGCC 3 cut(s) 1039, 1230, 1409
BtgZI GCGATG 3 cut(s) 147, 960, 1080
BtsCI GGATG 3 cut(s) 558, 831, 1353
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 4 cut(s) 399, 714, 841, 1483
BveI ACCTGC 2 cut(s) 803, 1264
Cac8I GCNNGC 5 cut(s) 16, 196, 359, 612, 1429
CfoI GCGC 3 cut(s) 20, 1065, 1439
Cfr10I RCCGGY 3 cut(s) 14, 62, 194
Cfr13I GGNCC 5 cut(s) 73, 171, 494, 536, 1229
CseI GACGC 1 cut(s) 708
CsiI ACCWGGT 1 cut(s) 173
Csp6I GTAC 3 cut(s) 301, 979, 1238
CviAII CATG 8 cut(s) 298, 530, 611, 929, 934, 982, 1189, 1357
CviQI GTAC 3 cut(s) 301, 979, 1238
DdeI CTNAG 2 cut(s) 634, 956
DinI GGCGCC 1 cut(s) 19
DpnI GATC 8 cut(s) 147, 191, 504, 528, 585, 1074, 1182, 1330
DpnII GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
DraIII CACNNNGTG 1 cut(s) 1325
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 1407
Eam1104I CTCTTC 2 cut(s) 318, 726
EarI CTCTTC 2 cut(s) 318, 726
Ecl136II GAGCTC 1 cut(s) 1458
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 2 cut(s) 957, 1460
Eco31I GGTCTC 1 cut(s) 785
Eco47I GGWCC 4 cut(s) 73, 171, 494, 536
Eco53kI GAGCTC 1 cut(s) 1458
Eco57I CTGAAG 3 cut(s) 278, 620, 1401
Eco88I CYCGRG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 1458
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 4 cut(s) 173, 538, 757, 1365
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 2 cut(s) 957, 1460
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 8 cut(s) 301, 533, 614, 932, 937, 985, 1192, 1360
FaqI GGGAC 4 cut(s) 86, 184, 1000, 1298
FatI CATG 8 cut(s) 297, 529, 610, 928, 933, 981, 1188, 1356
FauI CCCGC 1 cut(s) 366
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 5 cut(s) 60, 435, 615, 1265, 1268
FokI GGATG 3 cut(s) 565, 838, 1360
FriOI GRGCYC 2 cut(s) 957, 1460
Fsp4HI GCNGC 5 cut(s) 60, 435, 615, 1265, 1268
FspBI CTAG 2 cut(s) 561, 771
GlaI GCGC 3 cut(s) 19, 1064, 1438
GluI GCNGC 5 cut(s) 60, 435, 615, 1265, 1268
GsaI CCCAGC 1 cut(s) 1263
GsuI CTGGAG 4 cut(s) 561, 780, 1092, 1474
HaeII RGCGCY 2 cut(s) 21, 1440
HaeIII GGCC 3 cut(s) 1039, 1230, 1409
HapII CCGG 8 cut(s) 15, 63, 70, 195, 465, 618, 689, 1468
HgaI GACGC 1 cut(s) 708
HhaI GCGC 3 cut(s) 20, 1065, 1439
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 8 cut(s) 301, 533, 614, 932, 937, 985, 1192, 1360
Hin6I GCGC 3 cut(s) 18, 1063, 1437
HinP1I GCGC 3 cut(s) 18, 1063, 1437
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HindIII AAGCTT 3 cut(s) 108, 631, 651
HinfI GANTC 5 cut(s) 4, 117, 342, 659, 1473
HpaII CCGG 8 cut(s) 15, 63, 70, 195, 465, 618, 689, 1468
HphI GGTGA 3 cut(s) 815, 1034, 1315
Hpy166II GTNNAC 4 cut(s) 376, 421, 979, 1057
Hpy188I TCNGA 7 cut(s) 106, 189, 462, 588, 859, 1185, 1217
Hpy188III TCNNGA 9 cut(s) 114, 124, 143, 169, 598, 647, 663, 689, 1109
Hpy8I GTNNAC 4 cut(s) 376, 421, 979, 1057
Hpy99I CGWCG 5 cut(s) 26, 106, 161, 170, 724
HpyAV CCTTC 5 cut(s) 374, 472, 686, 1106, 1452
HpyCH4III ACNGT 4 cut(s) 102, 166, 418, 836
HpyCH4IV ACGT 3 cut(s) 87, 372, 1383
HpyCH4V TGCA 9 cut(s) 710, 728, 868, 937, 998, 1121, 1174, 1380, 1465
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 716, 725
HpyF3I CTNAG 2 cut(s) 634, 956
HpySE526I ACGT 3 cut(s) 87, 372, 1383
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 8 cut(s) 301, 533, 614, 932, 937, 985, 1192, 1360
HspAI GCGC 3 cut(s) 18, 1063, 1437
KasI GGCGCC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 688
KroI GCCGGC 2 cut(s) 14, 194
KroNI GCCGGC 2 cut(s) 16, 196
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
LmnI GCTCC 6 cut(s) 145, 203, 1111, 1399, 1455, 1463
Lsp1109I GCAGC 3 cut(s) 71, 601, 1276
LweI GCATC 4 cut(s) 127, 232, 543, 1057
MabI ACCWGGT 1 cut(s) 173
MaeI CTAG 2 cut(s) 561, 771
MaeII ACGT 3 cut(s) 87, 372, 1383
MaeIII GTNAC 7 cut(s) 206, 239, 368, 385, 412, 803, 836
MalI GATC 8 cut(s) 147, 191, 504, 528, 585, 1074, 1182, 1330
MboI GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
MflI RGATCY 1 cut(s) 1328
MhlI GDGCHC 2 cut(s) 957, 1460
MluCI AATT 4 cut(s) 78, 214, 910, 1218
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 1 cut(s) 351
MmeI TCCRAC 2 cut(s) 84, 1366
MroI TCCGGA 1 cut(s) 688
MroNI GCCGGC 2 cut(s) 14, 194
MseI TTAA 3 cut(s) 351, 888, 1293
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 8 cut(s) 15, 63, 70, 195, 465, 618, 689, 1468
MspR9I CCNGG 7 cut(s) 71, 175, 466, 540, 759, 1367, 1469
Mva1269I GAATGC 2 cut(s) 730, 1176
MvaI CCWGG 4 cut(s) 175, 540, 759, 1367
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 3 cut(s) 59, 716, 725
NaeI GCCGGC 2 cut(s) 16, 196
NarI GGCGCC 1 cut(s) 18
NciI CCSGG 3 cut(s) 71, 466, 1469
NdeII GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
NgoMIV GCCGGC 2 cut(s) 14, 194
NlaIII CATG 8 cut(s) 301, 533, 614, 932, 937, 985, 1192, 1360
NlaIV GGNNCC 8 cut(s) 19, 74, 163, 172, 199, 622, 1107, 1330
NmeAIII GCCGAG 1 cut(s) 1435
NmuCI GTSAC 4 cut(s) 239, 368, 803, 836
NspI RCATGY 2 cut(s) 614, 1360
PaeI GCATGC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 162
PctI GAATGC 2 cut(s) 730, 1176
PdiI GCCGGC 2 cut(s) 16, 196
PfeI GAWTC 3 cut(s) 117, 659, 1473
PfoI TCCNGGA 1 cut(s) 538
PkrI GCNGC 5 cut(s) 61, 436, 616, 1266, 1269
PleI GAGTC 1 cut(s) 350
PluTI GGCGCC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 1460
Psp1406I AACGTT 1 cut(s) 1383
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 4 cut(s) 173, 538, 757, 1365
PspFI CCCAGC 1 cut(s) 1259
PspGI CCWGG 4 cut(s) 173, 538, 757, 1365
PspN4I GGNNCC 8 cut(s) 19, 74, 163, 172, 199, 622, 1107, 1330
PspPI GGNCC 5 cut(s) 73, 171, 494, 536, 1229
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 1 cut(s) 1328
RsaI GTAC 3 cut(s) 302, 980, 1239
RsaNI GTAC 3 cut(s) 301, 979, 1238
SacI GAGCTC 1 cut(s) 1460
SaqAI TTAA 3 cut(s) 351, 888, 1293
SatI GCNGC 5 cut(s) 60, 435, 615, 1265, 1268
Sau3AI GATC 8 cut(s) 145, 189, 502, 526, 583, 1072, 1180, 1328
Sau96I GGNCC 5 cut(s) 73, 171, 494, 536, 1229
ScaI AGTACT 1 cut(s) 1239
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 7 cut(s) 71, 175, 466, 540, 759, 1367, 1469
SduI GDGCHC 2 cut(s) 957, 1460
SexAI ACCWGGT 1 cut(s) 173
SfaNI GCATC 4 cut(s) 127, 232, 543, 1057
SfoI GGCGCC 1 cut(s) 19
SinI GGWCC 4 cut(s) 73, 171, 494, 536
SmlI CTYRAG 3 cut(s) 850, 1033, 1292
SmoI CTYRAG 3 cut(s) 850, 1033, 1292
SpeI ACTAGT 1 cut(s) 560
SphI GCATGC 1 cut(s) 614
Sse9I AATT 4 cut(s) 78, 214, 910, 1218
SsiI CCGC 5 cut(s) 57, 359, 435, 492, 1268
SspDI GGCGCC 1 cut(s) 17
SspMI CTAG 2 cut(s) 561, 771
SstI GAGCTC 1 cut(s) 1460
StyD4I CCNGG 7 cut(s) 69, 173, 464, 538, 757, 1365, 1467
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 4 cut(s) 102, 166, 418, 836
TaiI ACGT 3 cut(s) 90, 375, 1386
TaqI TCGA 3 cut(s) 675, 722, 1252
TasI AATT 4 cut(s) 78, 214, 910, 1218
TatI WGTACW 2 cut(s) 978, 1237
TauI GCSGC 2 cut(s) 437, 1270
TfiI GAWTC 3 cut(s) 117, 659, 1473
Tru1I TTAA 3 cut(s) 351, 888, 1293
Tru9I TTAA 3 cut(s) 351, 888, 1293
TscAI CASTG 4 cut(s) 406, 721, 841, 1483
TseFI GTSAC 4 cut(s) 239, 368, 803, 836
TseI GCWGC 3 cut(s) 59, 614, 1264
Tsp45I GTSAC 4 cut(s) 239, 368, 803, 836
TspDTI ATGAA 7 cut(s) 286, 294, 502, 894, 1070, 1205, 1364
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 4 cut(s) 406, 721, 841, 1483
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 4 cut(s) 73, 171, 494, 536
XceI RCATGY 2 cut(s) 614, 1360
XspI CTAG 2 cut(s) 561, 771
ZrmI AGTACT 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.