pycom15g05820

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005609
Physical Location & Seq
Reverse (-)
3528861 .. 3530916
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGCAGAATTTGCAATCGAGGGCTGTGCCGTTAGAGGAAGGCAATGACCCTGGTGTTACTAGCAGCCGGGCAAGCCAAACGAAGGCCTTGCCAATTAGGCTACTCCAGTTATTCGCGCTGTTTCTGCTTTTCTGTATTACTTTCTCAGTCATTAGCGTATACACAATTCGGCATTTTGGAATCAATGGCGTCATGACAACAGCCGCGACCTCTTTCCAGCCGTGCCTTGAGGAACCAAGAGGTTTGGACCGATGGATTAAGCCGCCATCAAGTGTTAAACACACCATGAATGATCAGGAACTGTTCTGGAGGGCTTCTTTTACGCCTAGGATAAAGAAATATCCTTTTGAGAAAGTTCCAAAGATTGCATTTATGTTCTTGACTAAGGGGCCGTTGCCGCTGGCACCCCTTTGGGAGAGGTTTCTGAAGGGATACGAAAGGCTTTATTCAGTCTACGTTCATTCACTACCATCGTTTCAACCCCATTTTAATCCTTCGTCAGCTTTTTATGGGAGACATATTCCAAGCCAGGTTGCTGAGTGGGGAAGAATGAGTATGTGTGATGCTGAGAGAAGACTCCTTGCTAATGCGTTGCTCGACATATCCAATGAATGGTTCATTCTTCTTTCTGAATCATGCATTCCGCTCTACAATTTCAGTGTCGTTCACCACTACTTGATGAAGTCCAAGTACAGCTATGTCGGTGCATTTGATGACCCCGGGCCACATGGAAGAGGACGCTACAATGATAACATGGCACCTGAAGTGAACATATCCGATTGGCGCAAGGGCTCCCAGTGGTTTGAAGTTAACCGAAAGCTTGCCATCAGCATTGTCGAGGATACAAAATATTACCCCAAATTTAAAGAGTTCTGTAGACCCGCATGTTACGTTGATGAGCACTACTTCCCCACGATGCTAACCATTGAGGCCGGGAATTCTTTAGCAAATAGAAGCATTACTTGGGTGGATTGGTCACGGGGTGGACCTCACCCGGCTACCTTTGGAAGAGCGGACATCACGGAGGAATTTCTCAAGAGAATTTTCTGGGGTCAACGCTGCACTTACAATGGCCAGAACTCAACAATCTGCTTTCTTTTTGGAAGGAAGTTTGCTCCGAGTGCCATGGAACCTCTTTTGCATTTAGCACCAAAGTTTCTGGGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

390

Amino Acids

44.82

Weight (kDa)

9.15

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 122 - 348 7.9e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 403, 757
AccB7I CCANNNNNTGG 1 cut(s) 612
AccBSI CCGCTC 2 cut(s) 646, 1013
AccI GTMKAC 3 cut(s) 159, 453, 877
AccII CGCG 2 cut(s) 116, 206
AciI CCGC 6 cut(s) 204, 263, 398, 644, 882, 1013
AcoI YGGCCR 1 cut(s) 1072
AcsI RAATTY 5 cut(s) 7, 860, 937, 1028, 1041
AcuI CTGAAG 2 cut(s) 446, 783
AcyI GRCGYC 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 983
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 2 cut(s) 82, 612
AgsI TTSAA 2 cut(s) 479, 806
AjnI CCWGG 2 cut(s) 49, 528
AjuI GAANNNNNNNTTGG 4 cut(s) 599, 631, 946, 978
AluBI AGCT 3 cut(s) 503, 696, 820
AluI AGCT 3 cut(s) 503, 696, 820
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 1 cut(s) 508
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 719
AoxI GGCC 5 cut(s) 84, 389, 722, 930, 1072
ApeKI GCWGC 2 cut(s) 63, 1059
ApoI RAATTY 5 cut(s) 7, 860, 937, 1028, 1041
AspA2I CCTAGG 1 cut(s) 326
AspLEI GCGC 2 cut(s) 118, 786
AspS9I GGNCC 4 cut(s) 247, 389, 722, 986
AsuC2I CCSGG 5 cut(s) 68, 720, 721, 934, 995
AsuHPI GGTGA 2 cut(s) 659, 983
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 2 cut(s) 247, 986
AvrII CCTAGG 1 cut(s) 326
BalI TGGCCA 1 cut(s) 1074
BanI GGYRCC 2 cut(s) 403, 757
BanII GRGCYC 1 cut(s) 794
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 75, 1046
BccI CCATC 4 cut(s) 246, 274, 478, 833
BceAI ACGGC 3 cut(s) 13, 205, 376
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 70, 104
BciT130I CCWGG 2 cut(s) 51, 530
BciVI GTATCC 2 cut(s) 425, 835
BclI TGATCA 1 cut(s) 292
BcnI CCSGG 5 cut(s) 68, 720, 721, 934, 995
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 2 cut(s) 60, 327
BfmI CTRYAG 1 cut(s) 874
BfuI GTATCC 2 cut(s) 425, 835
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 5 cut(s) 64, 204, 263, 398, 1060
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 5 cut(s) 65, 205, 264, 399, 1061
Bme1390I CCNGG 7 cut(s) 51, 68, 530, 720, 721, 934, 995
Bme18I GGWCC 2 cut(s) 247, 986
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 4 cut(s) 247, 389, 722, 986
BmiI GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
BmrFI CCNGG 7 cut(s) 51, 68, 530, 720, 721, 934, 995
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 2 cut(s) 553, 906
BmuI ACTGGG 1 cut(s) 790
BpiI GAAGAC 1 cut(s) 580
BpmI CTGGAG 2 cut(s) 89, 328
BpuEI CTTGAG 2 cut(s) 248, 1019
BpuMI CCSGG 5 cut(s) 68, 720, 721, 934, 995
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 49, 326, 718, 719, 1125
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 612
Bse1I ACTGG 2 cut(s) 106, 796
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 2 cut(s) 51, 530
BseDI CCNNGG 5 cut(s) 49, 326, 718, 719, 1125
BseLI CCNNNNNNNGG 2 cut(s) 82, 612
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 3 cut(s) 159, 528, 558
BseNI ACTGG 2 cut(s) 106, 796
BseXI GCAGC 2 cut(s) 75, 1046
BsgI GTGCAG 1 cut(s) 1045
Bsh1236I CGCG 2 cut(s) 116, 206
BshFI GGCC 5 cut(s) 86, 391, 724, 932, 1074
BshNI GGYRCC 2 cut(s) 403, 757
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 4 cut(s) 67, 720, 933, 995
BslI CCNNNNNNNGG 2 cut(s) 82, 612
BsmAI GTCTC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 5 cut(s) 86, 391, 724, 932, 1074
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 2 cut(s) 794, 903
Bsp143I GATC 1 cut(s) 292
Bsp19I CCATGG 1 cut(s) 1125
BspACI CCGC 6 cut(s) 204, 263, 398, 644, 882, 1013
BspANI GGCC 5 cut(s) 86, 391, 724, 932, 1074
BspCNI CTCAG 3 cut(s) 158, 529, 559
BspFNI CGCG 2 cut(s) 116, 206
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
BspQI GCTCTTC 1 cut(s) 1003
BspT107I GGYRCC 2 cut(s) 403, 757
BsrBI CCGCTC 2 cut(s) 646, 1013
BsrDI GCAATG 1 cut(s) 49
BsrI ACTGG 2 cut(s) 106, 796
BssECI CCNNGG 5 cut(s) 49, 326, 718, 719, 1125
BssMI GATC 1 cut(s) 292
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 326, 1125
Bst1107I GTATAC 1 cut(s) 160
Bst2UI CCWGG 2 cut(s) 51, 530
Bst4CI ACNGT 1 cut(s) 303
Bst6I CTCTTC 2 cut(s) 727, 1003
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 73, 402, 822
BstDEI CTNAG 4 cut(s) 145, 384, 537, 567
BstDSI CCRYGG 1 cut(s) 1125
BstFNI CGCG 2 cut(s) 116, 206
BstHHI GCGC 2 cut(s) 118, 786
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 6 cut(s) 10, 72, 97, 124, 397, 1121
BstNI CCWGG 2 cut(s) 51, 530
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 7 cut(s) 49, 66, 528, 718, 719, 932, 993
BstSFI CTRYAG 1 cut(s) 874
BstUI CGCG 2 cut(s) 116, 206
BstV1I GCAGC 2 cut(s) 75, 1046
BstV2I GAAGAC 1 cut(s) 580
BstZ17I GTATAC 1 cut(s) 160
BsuI GTATCC 2 cut(s) 425, 835
BsuRI GGCC 5 cut(s) 86, 391, 724, 932, 1074
BtgI CCRYGG 1 cut(s) 1125
BtsIMutI CAGTG 2 cut(s) 664, 803
Cac8I GCNNGC 3 cut(s) 73, 402, 822
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 118, 786
Cfr13I GGNCC 4 cut(s) 247, 389, 722, 986
Cfr9I CCCGGG 1 cut(s) 719
CseI GACGC 2 cut(s) 178, 747
Csp6I GTAC 1 cut(s) 691
CviAII CATG 7 cut(s) 193, 286, 636, 728, 754, 885, 1126
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 4 cut(s) 145, 384, 537, 567
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
DraI TTTAAA 1 cut(s) 865
DraIII CACNNNGTG 1 cut(s) 983
EaeI YGGCCR 1 cut(s) 1072
Eam1104I CTCTTC 2 cut(s) 727, 1003
EarI CTCTTC 2 cut(s) 727, 1003
Eco130I CCWWGG 2 cut(s) 326, 1125
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 794
Eco47I GGWCC 2 cut(s) 247, 986
Eco57I CTGAAG 2 cut(s) 446, 783
Eco88I CYCGRG 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 937
EcoRII CCWGG 2 cut(s) 49, 528
EcoT14I CCWWGG 2 cut(s) 326, 1125
EcoT22I ATGCAT 1 cut(s) 641
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 326, 1125
FaeI CATG 7 cut(s) 196, 289, 639, 731, 757, 888, 1129
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FatI CATG 7 cut(s) 192, 285, 635, 727, 753, 884, 1125
FauI CCCGC 1 cut(s) 889
FbaI TGATCA 1 cut(s) 292
FblI GTMKAC 3 cut(s) 159, 453, 877
Fnu4HI GCNGC 5 cut(s) 64, 204, 263, 398, 1060
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 5 cut(s) 64, 204, 263, 398, 1060
FspBI CTAG 2 cut(s) 60, 327
GlaI GCGC 2 cut(s) 117, 785
GluI GCNGC 5 cut(s) 64, 204, 263, 398, 1060
GsuI CTGGAG 2 cut(s) 89, 328
HaeIII GGCC 5 cut(s) 86, 391, 724, 932, 1074
HapII CCGG 4 cut(s) 67, 720, 933, 995
HgaI GACGC 2 cut(s) 178, 747
HhaI GCGC 2 cut(s) 118, 786
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 7 cut(s) 196, 289, 639, 731, 757, 888, 1129
Hin6I GCGC 2 cut(s) 116, 784
HinP1I GCGC 2 cut(s) 116, 784
HincII GTYRAC 2 cut(s) 811, 1055
HindII GTYRAC 2 cut(s) 811, 1055
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 180, 576, 632
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 4 cut(s) 67, 720, 933, 995
HphI GGTGA 2 cut(s) 659, 983
Hpy166II GTNNAC 8 cut(s) 160, 454, 667, 769, 811, 878, 986, 1055
Hpy188I TCNGA 4 cut(s) 426, 631, 778, 1119
Hpy188III TCNNGA 5 cut(s) 193, 296, 307, 379, 1036
Hpy8I GTNNAC 8 cut(s) 160, 454, 667, 769, 811, 878, 986, 1055
HpyAV CCTTC 5 cut(s) 32, 76, 421, 504, 1098
HpyCH4III ACNGT 1 cut(s) 303
HpyCH4IV ACGT 2 cut(s) 456, 891
HpyCH4V TGCA 7 cut(s) 4, 13, 368, 639, 707, 1062, 1141
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 72, 97, 124, 397, 1121
HpyF3I CTNAG 4 cut(s) 145, 384, 537, 567
HpySE526I ACGT 2 cut(s) 456, 891
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 7 cut(s) 196, 289, 639, 731, 757, 888, 1129
HspAI GCGC 2 cut(s) 116, 784
Ksp22I TGATCA 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 1 cut(s) 292
LguI GCTCTTC 1 cut(s) 1003
LmnI GCTCC 2 cut(s) 797, 1120
Lsp1109I GCAGC 2 cut(s) 75, 1046
LweI GCATC 2 cut(s) 553, 906
MaeI CTAG 2 cut(s) 60, 327
MaeII ACGT 2 cut(s) 456, 891
MaeIII GTNAC 3 cut(s) 55, 887, 975
MalI GATC 1 cut(s) 294
MbiI CCGCTC 2 cut(s) 646, 1013
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 558, 585, 614, 744, 1020
MhlI GDGCHC 2 cut(s) 794, 903
MlsI TGGCCA 1 cut(s) 1074
MluCI AATT 8 cut(s) 7, 93, 165, 652, 860, 937, 1028, 1041
MluNI TGGCCA 1 cut(s) 1074
MlyI GAGTC 1 cut(s) 570
Mox20I TGGCCA 1 cut(s) 1074
Mph1103I ATGCAT 1 cut(s) 641
MscI TGGCCA 1 cut(s) 1074
MseI TTAA 5 cut(s) 258, 276, 489, 810, 864
Msp20I TGGCCA 1 cut(s) 1074
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 4 cut(s) 67, 720, 933, 995
MspR9I CCNGG 7 cut(s) 51, 68, 530, 720, 721, 934, 995
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 2 cut(s) 51, 530
MvnI CGCG 2 cut(s) 116, 206
MwoI GCNNNNNNNGC 6 cut(s) 10, 72, 97, 124, 397, 1121
NciI CCSGG 5 cut(s) 68, 720, 721, 934, 995
NcoI CCATGG 1 cut(s) 1125
NdeII GATC 1 cut(s) 292
NlaIII CATG 7 cut(s) 196, 289, 639, 731, 757, 888, 1129
NlaIV GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
NmuCI GTSAC 1 cut(s) 975
NsiI ATGCAT 1 cut(s) 641
NspI RCATGY 1 cut(s) 888
PagI TCATGA 1 cut(s) 192
PceI AGGCCT 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 1003
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 2 cut(s) 180, 632
PflMI CCANNNNNTGG 1 cut(s) 612
PkrI GCNGC 5 cut(s) 65, 205, 264, 399, 1061
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 49, 528
PspGI CCWGG 2 cut(s) 49, 528
PspN4I GGNNCC 6 cut(s) 234, 390, 405, 759, 793, 1131
PspPI GGNCC 4 cut(s) 247, 389, 722, 986
PstNI CAGNNNCTG 1 cut(s) 301
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
SapI GCTCTTC 1 cut(s) 1003
SaqAI TTAA 5 cut(s) 258, 276, 489, 810, 864
SatI GCNGC 5 cut(s) 64, 204, 263, 398, 1060
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 4 cut(s) 247, 389, 722, 986
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 7 cut(s) 51, 68, 530, 720, 721, 934, 995
SduI GDGCHC 2 cut(s) 794, 903
SfaNI GCATC 2 cut(s) 553, 906
SfcI CTRYAG 1 cut(s) 874
SinI GGWCC 2 cut(s) 247, 986
SmaI CCCGGG 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 227, 1034
SmoI CTYRAG 2 cut(s) 227, 1034
Sse9I AATT 8 cut(s) 7, 93, 165, 652, 860, 937, 1028, 1041
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 6 cut(s) 204, 263, 398, 644, 882, 1013
SspI AATATT 1 cut(s) 851
SspMI CTAG 2 cut(s) 60, 327
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 7 cut(s) 49, 66, 528, 718, 719, 932, 993
StyI CCWWGG 2 cut(s) 326, 1125
TaaI ACNGT 1 cut(s) 303
TaiI ACGT 2 cut(s) 459, 894
TaqI TCGA 3 cut(s) 17, 597, 837
TaqII GACCGA 1 cut(s) 264
TasI AATT 8 cut(s) 7, 93, 165, 652, 860, 937, 1028, 1041
TatI WGTACW 1 cut(s) 690
TauI GCSGC 3 cut(s) 206, 265, 400
TfiI GAWTC 2 cut(s) 180, 632
Tru1I TTAA 5 cut(s) 258, 276, 489, 810, 864
Tru9I TTAA 5 cut(s) 258, 276, 489, 810, 864
TscAI CASTG 2 cut(s) 664, 803
TseFI GTSAC 1 cut(s) 975
TseI GCWGC 2 cut(s) 63, 1059
Tsp45I GTSAC 1 cut(s) 975
TspDTI ATGAA 5 cut(s) 302, 449, 607, 624, 695
TspGWI ACGGA 1 cut(s) 1037
TspMI CCCGGG 1 cut(s) 719
TspRI CASTG 2 cut(s) 664, 803
Van91I CCANNNNNTGG 1 cut(s) 612
VpaK11BI GGWCC 2 cut(s) 247, 986
XapI RAATTY 5 cut(s) 7, 860, 937, 1028, 1041
XceI RCATGY 1 cut(s) 888
XmaI CCCGGG 1 cut(s) 719
XmaJI CCTAGG 1 cut(s) 326
XmiI GTMKAC 3 cut(s) 159, 453, 877
XspI CTAG 2 cut(s) 60, 327
Zsp2I ATGCAT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.