pycom15g07570

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005988
Physical Location & Seq
Forward (+)
4661694 .. 4664865
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1539 bp
ATGAAAAAAACAACTATGAGACATCGACACAATGCGGCTCATTTCCTCGCTTCCAAGCCAATTACTGTCATGGCTTCTAAGGAACATGTCTGTCACAATTATGTTTTCCTCAAGCCAGAAGAAGCAAGCTTCATCGAGCTCATTCGCCTCCTGTTTTCCTCCGACGTAGAAAAGAGAAGCTTCATCAACTGCTCGGAAGAGGAAGGATGTAAACGACGAAAACTTGAACACCGATGGCTAATCTTCGTCTCTATTGTGGTGCAATGGATGTTCCTTTTCTTCAGAACGCCCATGGCTCGAACTGGGGGTACCGTCAACAAGTTTCTAAATCTTGTAGCATCCAATGACGGATTGTTCGCGCTCTTCAAAATGTGGCTTAAAGGAAAGGTGGCAAAGGCAGATTCGTTATCAACCAGATCAGTGCTGGGAAATCTTGATAGGCGAGTGGAATTGGACGACAACATCAAACCAGGCAATACAAGATACAAAGGAGCTCTGTCCATGATGGCGGCTAAAATGTCATACGAAAACCAAGTCCTCATTAAAACAATCGTCACGGATCACTGGAAGATGAAATTCGTAGGGTTTTACAACTTTTGGAATGGTTATCAGAGACGAGATTCAACGCAAGCCTTCCTGTTCCAAGACACAACATCTGACCCCAACTTGATTGTGGTTGCATTTAGAGGCACCAACCCCTTTGATGCAGACGCATGGAGGACGGATTTCGACATCTCATGGTGCGAGTTTGCAGGACTCGGCAAGACCCACAGCGGCTTCATGAAAGCTCTTGGCATGCAGCCAAACAGAAGCTGGCCCCTTGAACAAGAAACACAAGAAGTCAACGCCGCTGAACAACTTCCTCATCAGTTTGCTTACTACAAAATCAGACAAATTCTGATGGATTTAATGCAAGAAAACAAGAATGCCAAGTTCATACTGACAGGCCACAGCTTGGGCGGGGCATTGGCTGTGCTGTTTGCCGCGGTGCTTGCAATGCACGACTACGAGGAGAAATATGCATGGTTGATGGGGAGGTTGGAAGGGGTGTACACTTTTGGACAGCCAAGGGTGGGGGATGAGAACTTTGCGGTGTTTATGAGGGAGAAGCTGAAAAATTATGGTGTGAAGTATATGAGGTATGTTTATTCCAATGATATGGTTCCTAGGATACCTTATGACGATAAAACTCTCTTGTTTAAGCACTTTGGTCCAAGCCTATATTTCAGCAGCTGCTATAAGGGGAAGGTTTTGGAGGAGGAACCCAACAAGAACTACTTCAATGTGTTCTGGTTCATACCAAAGTTCCTAAATGCATTGTGGGAGCTGATTAGGAGCTTCATACTGCCGTTAATAAGGGGTGCGGAATACAAGGAAAGCTGGTTTATGAGGATGCTTAGGGTAGTTGGTTTAGTAATTCCTGGATTGGCAGCTCATGCTCCTCAAGATTATGTCAACATTACTCGATTGGGATCCCTTCCTTCCCTTGCTCACGTCCAAAAACGTATTCCTCTAGTAAATAATTCTAAAGTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

513

Amino Acids

59.38

Weight (kDa)

9.44

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 225 - 394 6.9e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 308, 689
AccB7I CCANNNNNTGG 1 cut(s) 955
AccII CGCG 2 cut(s) 359, 986
AciI CCGC 9 cut(s) 35, 509, 774, 849, 960, 984, 986, 1091, 1364
AclWI GGATC 3 cut(s) 567, 1467, 1480
AcsI RAATTY 2 cut(s) 575, 894
AcuI CTGAAG 1 cut(s) 265
AfaI GTAC 2 cut(s) 310, 1052
AfiI CCNNNNNNNGG 2 cut(s) 955, 1073
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 5 cut(s) 227, 367, 624, 824, 1282
AjiI CACGTC 1 cut(s) 1495
AjnI CCWGG 2 cut(s) 469, 1420
AjuI GAANNNNNNNTTGG 2 cut(s) 1491, 1523
Alw21I GWGCWC 2 cut(s) 141, 496
Alw26I GTCTC 3 cut(s) 13, 253, 607
AlwI GGATC 3 cut(s) 567, 1467, 1480
AlwNI CAGNNNCTG 2 cut(s) 813, 1233
AoxI GGCC 2 cut(s) 815, 946
ApeKI GCWGC 4 cut(s) 799, 1230, 1233, 1430
ApoI RAATTY 2 cut(s) 575, 894
ArsI GACNNNNNNTTYG 4 cut(s) 338, 370, 519, 551
Asp700I GAANNNNTTC 2 cut(s) 858, 1277
Asp718I GGTACC 1 cut(s) 308
AspA2I CCTAGG 1 cut(s) 1166
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 2 cut(s) 816, 1211
AvaII GGWCC 1 cut(s) 1211
AvrII CCTAGG 1 cut(s) 1166
BamHI GGATCC 1 cut(s) 1472
BanI GGYRCC 2 cut(s) 308, 689
BanII GRGCYC 2 cut(s) 141, 496
Bbv12I GWGCWC 2 cut(s) 141, 496
BbvI GCAGC 4 cut(s) 811, 1220, 1242, 1442
BccI CCATC 4 cut(s) 228, 499, 895, 1024
BceAI ACGGC 1 cut(s) 1333
BciT130I CCWGG 2 cut(s) 471, 1422
BciVI GTATCC 1 cut(s) 1164
BcoDI GTCTC 3 cut(s) 13, 253, 607
BfaI CTAG 2 cut(s) 1167, 1514
BfuI GTATCC 1 cut(s) 1164
BisI GCNGC 9 cut(s) 36, 510, 775, 800, 849, 984, 1231, 1234, 1431
BlnI CCTAGG 1 cut(s) 1166
BlsI GCNGC 9 cut(s) 37, 511, 776, 801, 850, 985, 1232, 1235, 1432
Bme1390I CCNGG 2 cut(s) 471, 1422
Bme18I GGWCC 1 cut(s) 1211
BmgBI CACGTC 1 cut(s) 1495
BmgT120I GGNCC 2 cut(s) 816, 1211
BmiI GGNNCC 6 cut(s) 310, 691, 818, 1164, 1263, 1474
BmrFI CCNGG 2 cut(s) 471, 1422
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 347, 694, 1383
BmuI ACTGGG 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 1397
BpuEI CTTGAG 2 cut(s) 95, 1428
BsaBI GATNNNNATC 1 cut(s) 1471
BsaJI CCNNGG 4 cut(s) 291, 984, 1067, 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 955, 1073
Bse1I ACTGG 2 cut(s) 307, 569
Bse3DI GCAATG 2 cut(s) 269, 1002
Bse8I GATNNNNATC 1 cut(s) 1471
BseBI CCWGG 2 cut(s) 471, 1422
BseDI CCNNGG 4 cut(s) 291, 984, 1067, 1166
BseGI GGATG 5 cut(s) 212, 273, 338, 1084, 1398
BseJI GATNNNNATC 1 cut(s) 1471
BseLI CCNNNNNNNGG 2 cut(s) 955, 1073
BseMI GCAATG 2 cut(s) 269, 1002
BseNI ACTGG 2 cut(s) 307, 569
BseRI GAGGAG 3 cut(s) 1025, 1271, 1431
BseXI GCAGC 4 cut(s) 811, 1220, 1242, 1442
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 2 cut(s) 359, 986
BshFI GGCC 2 cut(s) 817, 948
BshNI GGYRCC 2 cut(s) 308, 689
BsiHKAI GWGCWC 2 cut(s) 141, 496
BslI CCNNNNNNNGG 2 cut(s) 955, 1073
BsmAI GTCTC 3 cut(s) 13, 253, 607
BsmBI CGTCTC 2 cut(s) 253, 607
BsmI GAATGC 1 cut(s) 931
BsnI GGCC 2 cut(s) 817, 948
Bsp1286I GDGCHC 2 cut(s) 141, 496
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 3 cut(s) 416, 559, 1472
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 9 cut(s) 35, 509, 774, 849, 960, 984, 986, 1091, 1364
BspANI GGCC 2 cut(s) 817, 948
BspFNI CGCG 2 cut(s) 359, 986
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 6 cut(s) 310, 691, 818, 1164, 1263, 1474
BspPI GGATC 3 cut(s) 567, 1467, 1480
BspQI GCTCTTC 1 cut(s) 368
BspT107I GGYRCC 2 cut(s) 308, 689
BsrDI GCAATG 2 cut(s) 269, 1002
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 307, 569
BssECI CCNNGG 4 cut(s) 291, 984, 1067, 1166
BssMI GATC 3 cut(s) 416, 559, 1472
BssT1I CCWWGG 3 cut(s) 291, 1067, 1166
Bst2UI CCWGG 2 cut(s) 471, 1422
Bst4CI ACNGT 2 cut(s) 67, 313
Bst6I CTCTTC 2 cut(s) 192, 368
BstAPI GCANNNNNTGC 1 cut(s) 1436
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 5 cut(s) 127, 630, 797, 815, 993
BstDEI CTNAG 2 cut(s) 78, 1397
BstDSI CCRYGG 2 cut(s) 291, 984
BstF5I GGATG 5 cut(s) 212, 273, 338, 1084, 1398
BstFNI CGCG 2 cut(s) 359, 986
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 419, 562, 1475
BstMAI GTCTC 3 cut(s) 13, 253, 607
BstMBI GATC 3 cut(s) 416, 559, 1472
BstMWI GCNNNNNNNGC 3 cut(s) 992, 997, 1436
BstNI CCWGG 2 cut(s) 471, 1422
BstNSI RCATGY 2 cut(s) 89, 799
BstSCI CCNGG 2 cut(s) 469, 1420
BstUI CGCG 2 cut(s) 359, 986
BstV1I GCAGC 4 cut(s) 811, 1220, 1242, 1442
BstX2I RGATCY 1 cut(s) 1472
BstXI CCANNNNNNTGG 1 cut(s) 1159
BstYI RGATCY 1 cut(s) 1472
BsuI GTATCC 1 cut(s) 1164
BsuRI GGCC 2 cut(s) 817, 948
BtgI CCRYGG 2 cut(s) 291, 984
BtrI CACGTC 1 cut(s) 1495
BtsCI GGATG 5 cut(s) 212, 273, 338, 1084, 1398
BtsIMutI CAGTG 2 cut(s) 426, 562
Cac8I GCNNGC 5 cut(s) 127, 630, 797, 815, 993
CaiI CAGNNNCTG 2 cut(s) 813, 1233
CciI TCATGA 1 cut(s) 780
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 2 cut(s) 816, 1211
Cfr42I CCGCGG 1 cut(s) 987
CseI GACGC 1 cut(s) 719
Csp6I GTAC 2 cut(s) 309, 1051
CviQI GTAC 2 cut(s) 309, 1051
DdeI CTNAG 2 cut(s) 78, 1397
DpnI GATC 3 cut(s) 418, 561, 1474
DpnII GATC 3 cut(s) 416, 559, 1472
Eam1104I CTCTTC 2 cut(s) 192, 368
EarI CTCTTC 2 cut(s) 192, 368
Ecl136II GAGCTC 2 cut(s) 139, 494
Eco130I CCWWGG 3 cut(s) 291, 1067, 1166
Eco24I GRGCYC 2 cut(s) 141, 496
Eco47I GGWCC 1 cut(s) 1211
Eco53kI GAGCTC 2 cut(s) 139, 494
Eco57I CTGAAG 1 cut(s) 265
EcoICRI GAGCTC 2 cut(s) 139, 494
EcoRII CCWGG 2 cut(s) 469, 1420
EcoT14I CCWWGG 3 cut(s) 291, 1067, 1166
EcoT22I ATGCAT 2 cut(s) 1024, 1318
EcoT38I GRGCYC 2 cut(s) 141, 496
ErhI CCWWGG 3 cut(s) 291, 1067, 1166
Esp3I CGTCTC 2 cut(s) 253, 607
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 1262, 1294
FauI CCCGC 1 cut(s) 953
Fnu4HI GCNGC 9 cut(s) 36, 510, 775, 800, 849, 984, 1231, 1234, 1431
FokI GGATG 5 cut(s) 219, 280, 325, 1091, 1405
FriOI GRGCYC 2 cut(s) 141, 496
Fsp4HI GCNGC 9 cut(s) 36, 510, 775, 800, 849, 984, 1231, 1234, 1431
FspBI CTAG 2 cut(s) 1167, 1514
GlaI GCGC 1 cut(s) 360
GluI GCNGC 9 cut(s) 36, 510, 775, 800, 849, 984, 1231, 1234, 1431
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 2 cut(s) 817, 948
HgaI GACGC 1 cut(s) 719
HhaI GCGC 1 cut(s) 361
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 3 cut(s) 316, 844, 1456
HindII GTYRAC 3 cut(s) 316, 844, 1456
HindIII AAGCTT 2 cut(s) 127, 178
HinfI GANTC 3 cut(s) 401, 620, 756
Hpy166II GTNNAC 6 cut(s) 212, 316, 844, 1051, 1053, 1456
Hpy188I TCNGA 7 cut(s) 163, 196, 284, 612, 658, 890, 900
Hpy188III TCNNGA 3 cut(s) 434, 781, 1445
Hpy8I GTNNAC 6 cut(s) 212, 316, 844, 1051, 1053, 1456
Hpy99I CGWCG 2 cut(s) 167, 219
HpyAV CCTTC 6 cut(s) 197, 643, 1037, 1240, 1487, 1491
HpyCH4III ACNGT 2 cut(s) 67, 313
HpyCH4IV ACGT 3 cut(s) 165, 1494, 1504
HpyF10VI GCNNNNNNNGC 3 cut(s) 992, 997, 1436
HpyF3I CTNAG 2 cut(s) 78, 1397
HpySE526I ACGT 3 cut(s) 165, 1494, 1504
HspAI GCGC 1 cut(s) 359
KpnI GGTACC 1 cut(s) 312
KspI CCGCGG 1 cut(s) 987
Kzo9I GATC 3 cut(s) 416, 559, 1472
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 4 cut(s) 491, 1324, 1335, 1444
Lsp1109I GCAGC 4 cut(s) 811, 1220, 1242, 1442
LweI GCATC 3 cut(s) 347, 694, 1383
MaeI CTAG 2 cut(s) 1167, 1514
MaeII ACGT 3 cut(s) 165, 1494, 1504
MaeIII GTNAC 2 cut(s) 92, 553
MalI GATC 3 cut(s) 418, 561, 1474
MboI GATC 3 cut(s) 416, 559, 1472
MboII GAAGA 6 cut(s) 131, 209, 235, 271, 355, 580
MflI RGATCY 1 cut(s) 1472
MhlI GDGCHC 2 cut(s) 141, 496
MluCI AATT 8 cut(s) 60, 97, 449, 575, 894, 1117, 1416, 1522
MlyI GAGTC 1 cut(s) 750
MmeI TCCRAC 2 cut(s) 186, 1020
Mph1103I ATGCAT 2 cut(s) 1024, 1318
MroXI GAANNNNTTC 2 cut(s) 858, 1277
MseI TTAA 6 cut(s) 378, 543, 908, 1200, 1352, 1537
MslI CAYNNNNRTG 1 cut(s) 99
MspA1I CMGCKG 4 cut(s) 774, 851, 986, 1233
MspR9I CCNGG 2 cut(s) 471, 1422
Mva1269I GAATGC 1 cut(s) 931
MvaI CCWGG 2 cut(s) 471, 1422
MvnI CGCG 2 cut(s) 359, 986
MwoI GCNNNNNNNGC 3 cut(s) 992, 997, 1436
NcoI CCATGG 1 cut(s) 291
NdeII GATC 3 cut(s) 416, 559, 1472
NlaIV GGNNCC 6 cut(s) 310, 691, 818, 1164, 1263, 1474
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 2 cut(s) 92, 553
NsiI ATGCAT 2 cut(s) 1024, 1318
NspI RCATGY 2 cut(s) 89, 799
PaeI GCATGC 1 cut(s) 799
PagI TCATGA 1 cut(s) 780
PciI ACATGT 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 368
PctI GAATGC 1 cut(s) 931
PdmI GAANNNNTTC 2 cut(s) 858, 1277
PfeI GAWTC 2 cut(s) 401, 620
PflMI CCANNNNNTGG 1 cut(s) 955
PfoI TCCNGGA 1 cut(s) 1420
PkrI GCNGC 9 cut(s) 37, 511, 776, 801, 850, 985, 1232, 1235, 1432
PleI GAGTC 1 cut(s) 750
PpsI GAGTC 1 cut(s) 750
PscI ACATGT 1 cut(s) 85
Psp124BI GAGCTC 2 cut(s) 141, 496
Psp6I CCWGG 2 cut(s) 469, 1420
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 469, 1420
PspN4I GGNNCC 6 cut(s) 310, 691, 818, 1164, 1263, 1474
PspPI GGNCC 2 cut(s) 816, 1211
PsrI GAACNNNNNNTAC 2 cut(s) 292, 324
PstNI CAGNNNCTG 2 cut(s) 813, 1233
PsuI RGATCY 1 cut(s) 1472
PvuII CAGCTG 1 cut(s) 1233
RsaI GTAC 2 cut(s) 310, 1052
RsaNI GTAC 2 cut(s) 309, 1051
RseI CAYNNNNRTG 1 cut(s) 99
SacI GAGCTC 2 cut(s) 141, 496
SacII CCGCGG 1 cut(s) 987
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 6 cut(s) 378, 543, 908, 1200, 1352, 1537
SatI GCNGC 9 cut(s) 36, 510, 775, 800, 849, 984, 1231, 1234, 1431
Sau3AI GATC 3 cut(s) 416, 559, 1472
Sau96I GGNCC 2 cut(s) 816, 1211
SchI GAGTC 1 cut(s) 750
ScrFI CCNGG 2 cut(s) 471, 1422
SduI GDGCHC 2 cut(s) 141, 496
SfaNI GCATC 3 cut(s) 347, 694, 1383
Sfr303I CCGCGG 1 cut(s) 987
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 1 cut(s) 1211
SmiMI CAYNNNNRTG 1 cut(s) 99
SmlI CTYRAG 2 cut(s) 110, 1443
SmoI CTYRAG 2 cut(s) 110, 1443
SphI GCATGC 1 cut(s) 799
Sse9I AATT 8 cut(s) 60, 97, 449, 575, 894, 1117, 1416, 1522
SsiI CCGC 9 cut(s) 35, 509, 774, 849, 960, 984, 986, 1091, 1364
SspMI CTAG 2 cut(s) 1167, 1514
SstI GAGCTC 2 cut(s) 141, 496
StyD4I CCNGG 2 cut(s) 469, 1420
StyI CCWWGG 3 cut(s) 291, 1067, 1166
TaaI ACNGT 2 cut(s) 67, 313
TaiI ACGT 3 cut(s) 168, 1497, 1507
TaqI TCGA 6 cut(s) 25, 135, 298, 729, 1465, 1533
TasI AATT 8 cut(s) 60, 97, 449, 575, 894, 1117, 1416, 1522
TatI WGTACW 1 cut(s) 1050
TauI GCSGC 5 cut(s) 38, 512, 777, 851, 986
TfiI GAWTC 2 cut(s) 401, 620
Tru1I TTAA 6 cut(s) 378, 543, 908, 1200, 1352, 1537
Tru9I TTAA 6 cut(s) 378, 543, 908, 1200, 1352, 1537
TscAI CASTG 2 cut(s) 426, 569
TseFI GTSAC 2 cut(s) 92, 553
TseI GCWGC 4 cut(s) 799, 1230, 1233, 1430
Tsp45I GTSAC 2 cut(s) 92, 553
TspDTI ATGAA 9 cut(s) 17, 121, 172, 587, 769, 797, 925, 1285, 1330
TspGWI ACGGA 3 cut(s) 363, 572, 737
TspRI CASTG 2 cut(s) 426, 569
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 1211
XapI RAATTY 2 cut(s) 575, 894
XceI RCATGY 2 cut(s) 89, 799
XcmI CCANNNNNNNNNTGG 3 cut(s) 421, 670, 810
XmaJI CCTAGG 1 cut(s) 1166
XmnI GAANNNNTTC 2 cut(s) 858, 1277
XspI CTAG 2 cut(s) 1167, 1514
Zsp2I ATGCAT 2 cut(s) 1024, 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.