pycom15g07590

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005988
Physical Location & Seq
Forward (+)
4676331 .. 4679271
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1470 bp
ATGGCTTCTAAGGAACATGTCTGTCACAATTATGTTTTCCTCAAGCCAGAAGAAGCAAGCTTCATCGAGCTCATTCGCCTCCTGTTTTCCTCCGACGTAGAAAAGAGAAGCTTCATCAACTGCTCGGAAGAGGAAGGACGTAAACGACGAAAACTTGAACGCCGATGGCTAATCTTCGTCTCTATTGTGGTGCAATGGATGTTCCTTTTCTTCAGAACGCCCATGGCTCGAACTGGGGCTACCGTCAACAAGTTTCTAAATCTTGTAGCATCCAATGGCGGATTGTTCGCGCTCTTCAAAATGTGGCTTAAAGGAAAGGTGGCAAAGGCAGATTCGTTAACAACCAGATCACTGCTGGGAAATCTTGATAGGCGAGTGGAATTGGACGACAACATCAAACCAGGCAATACAAGATACAAAGGAGCTCTGTCCATGATGGCGGCTAAAATGTCATACGAAAACCAAGTCCTCATTAAAACAATCGTCACGGATCACTGGAAGATGAAATTCGTAGAGTTTTACAACTTTTGGAATGGTTATCAGAGACGAGATTCAACGCAAGCCTTCCTGTTCCAAGACACAACATCTGACCCCAACTTGATTGTGGTTGCATTTAGAGGCACCAACCCCTTTGATGCAGACGCATGGAGGACGGATTTCGACATCTCATGGTGCGAGTTTGCAGGACTCGGCAAGACCCACAGCGGCTTCATGAAAGCTCTTGGCATGCAACCAAACAGAAGCTGGCCCCTTGAACAAGAAACACAAGAAGTCAACGCCGCTGAACAACTTCCTCATCAGTTTGCTTACTACAAAATCAGACAAATTCTGATGGATTTAATGCAAGAAAACAAGAATGCCAAGTTCATACTGACAGGCCACAGCTTGGGCGGGGCATTGGCTGTGCTGTTTGCCGCGGTGCTTGCAATGCACGACTACGAGGAGAAATATGCATGGTTGATGGGGAGGTTGGAAGGGGTGTACACTTTTGGACAGCCAAGGGTGGGGGATGAGAACTTTGGGGTGTTTATGAGGGAGAAGCTGAAAAATTATGGTGTGAAGTATATGAGGTATGTTTATTCCAATGATATGGTTCCTAGGATACCTTATGACGATAAAACTCTCTTGTTTAAGCACTTTGGTCCAAGCCTATATTTCAGCAGCTGCTATAAGGGGAAGGTTTTGGAGGAGGAACCCAACAAGAACTACTTCAATGTGTTCTGGTTCATACCAAAGTGCCTAAATGCATTGTGGGAGCTGATTAGGAGCTTCATACTGCCGTTAATAAGGGGTGCGGAATACAAGGAAAGCTGGTTTATGAGGATGCTTAGGGTAGTTGGTTTAGTAATTCCTGGATTGGCAGCTCATGCTCCTCAAGATTATGTCAACATTACTCGATTGGGATCCCTTCCTTCCCTTGCTCACGTCCAAAAACGTATTCCTCTAGTAAATAATTCTAATGTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

490

Amino Acids

56.78

Weight (kDa)

9.29

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 202 - 371 2.9e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 620
AccB7I CCANNNNNTGG 1 cut(s) 886
AccII CGCG 2 cut(s) 290, 917
AciI CCGC 8 cut(s) 279, 440, 705, 780, 891, 915, 917, 1295
AclWI GGATC 3 cut(s) 498, 1398, 1411
AcsI RAATTY 2 cut(s) 506, 825
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 1 cut(s) 983
AfiI CCNNNNNNNGG 2 cut(s) 886, 1004
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 5 cut(s) 158, 298, 555, 755, 1213
AjiI CACGTC 1 cut(s) 1426
AjnI CCWGG 2 cut(s) 400, 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 1422, 1454
Alw21I GWGCWC 2 cut(s) 72, 427
Alw26I GTCTC 2 cut(s) 184, 538
AlwI GGATC 3 cut(s) 498, 1398, 1411
AlwNI CAGNNNCTG 2 cut(s) 744, 1164
AoxI GGCC 2 cut(s) 746, 877
ApeKI GCWGC 3 cut(s) 1161, 1164, 1361
ApoI RAATTY 2 cut(s) 506, 825
ArsI GACNNNNNNTTYG 2 cut(s) 450, 482
Asp700I GAANNNNTTC 2 cut(s) 789, 1208
AspA2I CCTAGG 1 cut(s) 1097
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 747, 1142
AvaII GGWCC 1 cut(s) 1142
AvrII CCTAGG 1 cut(s) 1097
BamHI GGATCC 1 cut(s) 1403
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 2 cut(s) 72, 427
Bbv12I GWGCWC 2 cut(s) 72, 427
BbvI GCAGC 3 cut(s) 1151, 1173, 1373
BccI CCATC 4 cut(s) 159, 430, 826, 955
BceAI ACGGC 1 cut(s) 1264
BciT130I CCWGG 2 cut(s) 402, 1353
BciVI GTATCC 1 cut(s) 1095
BcoDI GTCTC 2 cut(s) 184, 538
BfaI CTAG 2 cut(s) 1098, 1445
BfuI GTATCC 1 cut(s) 1095
BisI GCNGC 7 cut(s) 441, 706, 780, 915, 1162, 1165, 1362
BlnI CCTAGG 1 cut(s) 1097
BlsI GCNGC 7 cut(s) 442, 707, 781, 916, 1163, 1166, 1363
Bme1390I CCNGG 2 cut(s) 402, 1353
Bme18I GGWCC 1 cut(s) 1142
BmgBI CACGTC 1 cut(s) 1426
BmgT120I GGNCC 2 cut(s) 747, 1142
BmiI GGNNCC 5 cut(s) 622, 749, 1095, 1194, 1405
BmrFI CCNGG 2 cut(s) 402, 1353
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 3 cut(s) 278, 625, 1314
BmuI ACTGGG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 1328
BpuEI CTTGAG 2 cut(s) 26, 1359
BsaBI GATNNNNATC 1 cut(s) 1402
BsaJI CCNNGG 4 cut(s) 222, 915, 998, 1097
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 1004
Bse1I ACTGG 2 cut(s) 238, 500
Bse3DI GCAATG 2 cut(s) 200, 933
Bse8I GATNNNNATC 1 cut(s) 1402
BseBI CCWGG 2 cut(s) 402, 1353
BseDI CCNNGG 4 cut(s) 222, 915, 998, 1097
BseGI GGATG 4 cut(s) 204, 269, 1015, 1329
BseJI GATNNNNATC 1 cut(s) 1402
BseLI CCNNNNNNNGG 2 cut(s) 886, 1004
BseMI GCAATG 2 cut(s) 200, 933
BseNI ACTGG 2 cut(s) 238, 500
BseRI GAGGAG 3 cut(s) 956, 1202, 1362
BseXI GCAGC 3 cut(s) 1151, 1173, 1373
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 2 cut(s) 290, 917
BshFI GGCC 2 cut(s) 748, 879
BshNI GGYRCC 1 cut(s) 620
BsiHKAI GWGCWC 2 cut(s) 72, 427
BslI CCNNNNNNNGG 2 cut(s) 886, 1004
BsmAI GTCTC 2 cut(s) 184, 538
BsmBI CGTCTC 2 cut(s) 184, 538
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 2 cut(s) 748, 879
Bsp1286I GDGCHC 2 cut(s) 72, 427
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 3 cut(s) 347, 490, 1403
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 8 cut(s) 279, 440, 705, 780, 891, 915, 917, 1295
BspANI GGCC 2 cut(s) 748, 879
BspFNI CGCG 2 cut(s) 290, 917
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 5 cut(s) 622, 749, 1095, 1194, 1405
BspPI GGATC 3 cut(s) 498, 1398, 1411
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 620
BsrDI GCAATG 2 cut(s) 200, 933
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 2 cut(s) 238, 500
BssECI CCNNGG 4 cut(s) 222, 915, 998, 1097
BssMI GATC 3 cut(s) 347, 490, 1403
BssT1I CCWWGG 3 cut(s) 222, 998, 1097
Bst2UI CCWGG 2 cut(s) 402, 1353
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 2 cut(s) 123, 299
BstAPI GCANNNNNTGC 1 cut(s) 1367
BstAUI TGTACA 1 cut(s) 981
BstC8I GCNNGC 5 cut(s) 58, 561, 728, 746, 924
BstDEI CTNAG 2 cut(s) 9, 1328
BstDSI CCRYGG 2 cut(s) 222, 915
BstF5I GGATG 4 cut(s) 204, 269, 1015, 1329
BstFNI CGCG 2 cut(s) 290, 917
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 3 cut(s) 350, 493, 1406
BstMAI GTCTC 2 cut(s) 184, 538
BstMBI GATC 3 cut(s) 347, 490, 1403
BstMWI GCNNNNNNNGC 3 cut(s) 923, 928, 1367
BstNI CCWGG 2 cut(s) 402, 1353
BstNSI RCATGY 2 cut(s) 20, 730
BstSCI CCNGG 2 cut(s) 400, 1351
BstUI CGCG 2 cut(s) 290, 917
BstV1I GCAGC 3 cut(s) 1151, 1173, 1373
BstX2I RGATCY 1 cut(s) 1403
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1403
BsuI GTATCC 1 cut(s) 1095
BsuRI GGCC 2 cut(s) 748, 879
BtgI CCRYGG 2 cut(s) 222, 915
BtrI CACGTC 1 cut(s) 1426
BtsCI GGATG 4 cut(s) 204, 269, 1015, 1329
BtsI GCAGTG 1 cut(s) 350
BtsIMutI CAGTG 2 cut(s) 350, 493
Cac8I GCNNGC 5 cut(s) 58, 561, 728, 746, 924
CaiI CAGNNNCTG 2 cut(s) 744, 1164
CciI TCATGA 1 cut(s) 711
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 747, 1142
Cfr42I CCGCGG 1 cut(s) 918
CseI GACGC 1 cut(s) 650
Csp6I GTAC 1 cut(s) 982
CviAII CATG 9 cut(s) 17, 223, 433, 645, 669, 712, 727, 954, 1367
CviQI GTAC 1 cut(s) 982
DdeI CTNAG 2 cut(s) 9, 1328
DpnI GATC 3 cut(s) 349, 492, 1405
DpnII GATC 3 cut(s) 347, 490, 1403
Eam1104I CTCTTC 2 cut(s) 123, 299
EarI CTCTTC 2 cut(s) 123, 299
EciI GGCGGA 1 cut(s) 294
Ecl136II GAGCTC 2 cut(s) 70, 425
Eco130I CCWWGG 3 cut(s) 222, 998, 1097
Eco24I GRGCYC 2 cut(s) 72, 427
Eco47I GGWCC 1 cut(s) 1142
Eco53kI GAGCTC 2 cut(s) 70, 425
Eco57I CTGAAG 1 cut(s) 196
EcoICRI GAGCTC 2 cut(s) 70, 425
EcoRII CCWGG 2 cut(s) 400, 1351
EcoT14I CCWWGG 3 cut(s) 222, 998, 1097
EcoT22I ATGCAT 2 cut(s) 955, 1249
EcoT38I GRGCYC 2 cut(s) 72, 427
ErhI CCWWGG 3 cut(s) 222, 998, 1097
Esp3I CGTCTC 2 cut(s) 184, 538
FaeI CATG 9 cut(s) 20, 226, 436, 648, 672, 715, 730, 957, 1370
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1193, 1225
FatI CATG 9 cut(s) 16, 222, 432, 644, 668, 711, 726, 953, 1366
FauI CCCGC 1 cut(s) 884
Fnu4HI GCNGC 7 cut(s) 441, 706, 780, 915, 1162, 1165, 1362
FokI GGATG 4 cut(s) 211, 256, 1022, 1336
FriOI GRGCYC 2 cut(s) 72, 427
Fsp4HI GCNGC 7 cut(s) 441, 706, 780, 915, 1162, 1165, 1362
FspBI CTAG 2 cut(s) 1098, 1445
GlaI GCGC 1 cut(s) 291
GluI GCNGC 7 cut(s) 441, 706, 780, 915, 1162, 1165, 1362
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 2 cut(s) 748, 879
HgaI GACGC 1 cut(s) 650
HhaI GCGC 1 cut(s) 292
Hin1II CATG 9 cut(s) 20, 226, 436, 648, 672, 715, 730, 957, 1370
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 4 cut(s) 247, 339, 775, 1387
HindII GTYRAC 4 cut(s) 247, 339, 775, 1387
HindIII AAGCTT 2 cut(s) 58, 109
HinfI GANTC 3 cut(s) 332, 551, 687
HpaI GTTAAC 1 cut(s) 339
Hpy166II GTNNAC 7 cut(s) 143, 247, 339, 775, 982, 984, 1387
Hpy188I TCNGA 7 cut(s) 94, 127, 215, 543, 589, 821, 831
Hpy188III TCNNGA 3 cut(s) 365, 712, 1376
Hpy8I GTNNAC 7 cut(s) 143, 247, 339, 775, 982, 984, 1387
Hpy99I CGWCG 2 cut(s) 98, 150
HpyAV CCTTC 6 cut(s) 128, 574, 968, 1171, 1418, 1422
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 4 cut(s) 96, 139, 1425, 1435
HpyF10VI GCNNNNNNNGC 3 cut(s) 923, 928, 1367
HpyF3I CTNAG 2 cut(s) 9, 1328
HpySE526I ACGT 4 cut(s) 96, 139, 1425, 1435
Hsp92II CATG 9 cut(s) 20, 226, 436, 648, 672, 715, 730, 957, 1370
HspAI GCGC 1 cut(s) 290
KspAI GTTAAC 1 cut(s) 339
KspI CCGCGG 1 cut(s) 918
Kzo9I GATC 3 cut(s) 347, 490, 1403
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 4 cut(s) 422, 1255, 1266, 1375
Lsp1109I GCAGC 3 cut(s) 1151, 1173, 1373
LweI GCATC 3 cut(s) 278, 625, 1314
MaeI CTAG 2 cut(s) 1098, 1445
MaeII ACGT 4 cut(s) 96, 139, 1425, 1435
MaeIII GTNAC 2 cut(s) 23, 484
MalI GATC 3 cut(s) 349, 492, 1405
MboI GATC 3 cut(s) 347, 490, 1403
MboII GAAGA 6 cut(s) 62, 140, 166, 202, 286, 511
MflI RGATCY 1 cut(s) 1403
MhlI GDGCHC 2 cut(s) 72, 427
MluCI AATT 7 cut(s) 28, 380, 506, 825, 1048, 1347, 1453
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 2 cut(s) 117, 951
Mph1103I ATGCAT 2 cut(s) 955, 1249
MroXI GAANNNNTTC 2 cut(s) 789, 1208
MseI TTAA 7 cut(s) 309, 338, 474, 839, 1131, 1283, 1468
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 4 cut(s) 705, 782, 917, 1164
MspR9I CCNGG 2 cut(s) 402, 1353
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 2 cut(s) 402, 1353
MvnI CGCG 2 cut(s) 290, 917
MwoI GCNNNNNNNGC 3 cut(s) 923, 928, 1367
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 347, 490, 1403
NlaIII CATG 9 cut(s) 20, 226, 436, 648, 672, 715, 730, 957, 1370
NlaIV GGNNCC 5 cut(s) 622, 749, 1095, 1194, 1405
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 2 cut(s) 23, 484
NsiI ATGCAT 2 cut(s) 955, 1249
NspI RCATGY 2 cut(s) 20, 730
PaeI GCATGC 1 cut(s) 730
PagI TCATGA 1 cut(s) 711
PciI ACATGT 1 cut(s) 16
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 145
PctI GAATGC 1 cut(s) 862
PdmI GAANNNNTTC 2 cut(s) 789, 1208
PfeI GAWTC 2 cut(s) 332, 551
PflMI CCANNNNNTGG 1 cut(s) 886
PfoI TCCNGGA 1 cut(s) 1351
PkrI GCNGC 7 cut(s) 442, 707, 781, 916, 1163, 1166, 1363
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
PscI ACATGT 1 cut(s) 16
Psp124BI GAGCTC 2 cut(s) 72, 427
Psp6I CCWGG 2 cut(s) 400, 1351
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 2 cut(s) 400, 1351
PspN4I GGNNCC 5 cut(s) 622, 749, 1095, 1194, 1405
PspPI GGNCC 2 cut(s) 747, 1142
PsrI GAACNNNNNNTAC 2 cut(s) 223, 255
PstNI CAGNNNCTG 2 cut(s) 744, 1164
PsuI RGATCY 1 cut(s) 1403
PvuII CAGCTG 1 cut(s) 1164
RsaI GTAC 1 cut(s) 983
RsaNI GTAC 1 cut(s) 982
RseI CAYNNNNRTG 1 cut(s) 30
SacI GAGCTC 2 cut(s) 72, 427
SacII CCGCGG 1 cut(s) 918
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 7 cut(s) 309, 338, 474, 839, 1131, 1283, 1468
SatI GCNGC 7 cut(s) 441, 706, 780, 915, 1162, 1165, 1362
Sau3AI GATC 3 cut(s) 347, 490, 1403
Sau96I GGNCC 2 cut(s) 747, 1142
SchI GAGTC 1 cut(s) 681
ScrFI CCNGG 2 cut(s) 402, 1353
SduI GDGCHC 2 cut(s) 72, 427
SfaNI GCATC 3 cut(s) 278, 625, 1314
Sfr303I CCGCGG 1 cut(s) 918
SgrBI CCGCGG 1 cut(s) 918
SinI GGWCC 1 cut(s) 1142
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 2 cut(s) 41, 1374
SmoI CTYRAG 2 cut(s) 41, 1374
SphI GCATGC 1 cut(s) 730
Sse9I AATT 7 cut(s) 28, 380, 506, 825, 1048, 1347, 1453
SsiI CCGC 8 cut(s) 279, 440, 705, 780, 891, 915, 917, 1295
SspMI CTAG 2 cut(s) 1098, 1445
SstI GAGCTC 2 cut(s) 72, 427
StyD4I CCNGG 2 cut(s) 400, 1351
StyI CCWWGG 3 cut(s) 222, 998, 1097
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 4 cut(s) 99, 142, 1428, 1438
TaqI TCGA 5 cut(s) 66, 229, 660, 1396, 1464
TasI AATT 7 cut(s) 28, 380, 506, 825, 1048, 1347, 1453
TatI WGTACW 1 cut(s) 981
TauI GCSGC 4 cut(s) 443, 708, 782, 917
TfiI GAWTC 2 cut(s) 332, 551
Tru1I TTAA 7 cut(s) 309, 338, 474, 839, 1131, 1283, 1468
Tru9I TTAA 7 cut(s) 309, 338, 474, 839, 1131, 1283, 1468
TscAI CASTG 2 cut(s) 357, 500
TseFI GTSAC 2 cut(s) 23, 484
TseI GCWGC 3 cut(s) 1161, 1164, 1361
Tsp45I GTSAC 2 cut(s) 23, 484
TspDTI ATGAA 8 cut(s) 52, 103, 518, 700, 728, 856, 1216, 1261
TspGWI ACGGA 2 cut(s) 503, 668
TspRI CASTG 2 cut(s) 357, 500
Van91I CCANNNNNTGG 1 cut(s) 886
VpaK11BI GGWCC 1 cut(s) 1142
XapI RAATTY 2 cut(s) 506, 825
XceI RCATGY 2 cut(s) 20, 730
XcmI CCANNNNNNNNNTGG 3 cut(s) 352, 601, 741
XmaJI CCTAGG 1 cut(s) 1097
XmnI GAANNNNTTC 2 cut(s) 789, 1208
XspI CTAG 2 cut(s) 1098, 1445
Zsp2I ATGCAT 2 cut(s) 955, 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.