pycom15g07600

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005988
Physical Location & Seq
Forward (+)
4695114 .. 4697999
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1482 bp
ATGGACATAATCCAATTGCCAATTACTATCATGGCTTCTAAGGGACCTGTCTGTCTCAGTTATGTATTTCTCAAGCCCGAAGAAGCAACTTTCATCGAGCTCATTCGCCTCCTCTTTTCCTCTGACATAGAAAAGAGAAACTTCATCACCTCCTCAGCAGTAAGAAAACTTGACTGCAAACGGAGGTTGTTAATCTTCTTCTCTACCGTGATTCAATGCATCCTCCTTCTCTTCAGAAAACGCATGGCTTGGCTTGGGGGTGTTCTGGAGAAGTGGCTAAATCTTGTATCAAGCAACAGTGGATTGCTCATGCTCATCCTAAACTGGCTAAAAGGAAAGGTGGTGATACCAGATACATCATCAACCAAATCCCTGCTTGGATTTCTTGACACACGAATTGAATTAGACGACAACATGAAACCTGACGACACAAGATATAAAGGGTCGCTGTCCATGATGGCAGCCAAATTGTCGTACGAGAATGAAGAATTCACAGAAAAAGTTGTCAGTGAATACTGGAAGATGAAACTCGTGGCGTTCTACAAATTTTGGAATGGTTACGAGAAACGAGAATCAACCCAAGCCTTCGTGTTCCAAGACACAACATCTGACCCCAACTTGGTTTGGGTCGCATTTAGAGGCACTGAACCATTCAGCGCCGACGATTGGAGGACAGATTTTGACATCTCATGGTGCGAGTTTGCCGGAATCGGCAAGACCCACGGAGGCTTCATGAAAGCTCTCGGCATGCAAACAGAGCAAAGCTGGCCCCTCGAACCCGAAACAGAAGATCATGAGTTCGCTTACTACAAAATCAGACAAATTCTGATGGATTTATTGCAAGAAAACAAGAATGCCAAATTTATATTGACAGGTCACAGCTTGGGAGGGGCACTGGCGGTGCTGTTTGCGTCGGTGCTGGCAATGCATGACTACGAGGAGAAATATGCATGGTTGATGGAGAAGCTGGAGGGGGTGTACACCTTTGGACAGCCGAGGGTGGGGGATGAGAAATTTGCGGGCTATGTGACGAAGAAGCTGAAAAATTATGGGGTGAGGTATATGAGGTATGTTTATTCCAATGACTTGGTGCCTAGGGTACCATTTGATGATACAACCCTATTGTTTAAGCACGTTGCTCCATGCCTTTATTTCAACAGCTTCTATAAAGGGAAGGAGTTGGAGGAGGCACCCAACAAGAACTACTTCAATTTGCTATTCTTCATACCGATGCGGTTAAATGCATTGTGGGAGCTGATTAGGAGTTTCATACTCCCATTAATAAAGGGTTCAGAATACAAGGAAAGCTGGCTTATGATAATGGCTAGGTTTTTTGGTTTAATATTTCCTGGATTGGTAGCTCATGCTCCTCAAGATTATGTCAATGTTACTCGATTGGGATCGATCCCGTCCCACATGCCACTCCCAAAAGCTTACCTCCAGAAAATAAATCTAAAGTTAATTCAAGTTATGTTAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

494

Amino Acids

57.15

Weight (kDa)

8.5

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 210 - 370 3.7e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 51
Acc65I GGTACC 1 cut(s) 1099
AccB1I GGYRCC 3 cut(s) 1090, 1099, 1189
AciI CCGC 3 cut(s) 899, 1019, 1234
AclWI GGATC 2 cut(s) 1399, 1408
AcsI RAATTY 5 cut(s) 488, 545, 822, 860, 1013
AcuI CTGAAG 1 cut(s) 217
AfaI GTAC 3 cut(s) 476, 980, 1101
AfiI CCNNNNNNNGG 3 cut(s) 619, 666, 1001
AgsI TTSAA 5 cut(s) 215, 401, 1156, 1210, 1466
AjnI CCWGG 1 cut(s) 1348
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 2 cut(s) 1399, 1408
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 5 cut(s) 488, 545, 822, 860, 1013
AseI ATTAAT 1 cut(s) 1280
Asp700I GAANNNNTTC 1 cut(s) 1205
Asp718I GGTACC 1 cut(s) 1099
AspA2I CCTAGG 1 cut(s) 1094
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 44, 768
AsuHPI GGTGA 3 cut(s) 139, 355, 1066
AvaII GGWCC 1 cut(s) 44
AvrII CCTAGG 1 cut(s) 1094
BaeGI GKGCMC 1 cut(s) 895
BanI GGYRCC 3 cut(s) 1090, 1099, 1189
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 473
BccI CCATC 3 cut(s) 451, 823, 952
BciT130I CCWGG 1 cut(s) 1350
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 3 cut(s) 1095, 1326, 1480
BfoI RGCGCY 1 cut(s) 660
BisI GCNGC 1 cut(s) 462
BlnI CCTAGG 1 cut(s) 1094
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 1350
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 44, 768
BmiI GGNNCC 5 cut(s) 45, 770, 1092, 1101, 1191
BmrFI CCNGG 1 cut(s) 1350
BmsI GCATC 2 cut(s) 228, 1221
BpmI CTGGAG 3 cut(s) 287, 989, 1424
Bpu10I CCTNAGC 1 cut(s) 154
BpuEI CTTGAG 2 cut(s) 56, 1356
Bsa29I ATCGAT 1 cut(s) 1403
BsaBI GATNNNNATC 1 cut(s) 1399
BsaJI CCNNGG 3 cut(s) 721, 995, 1094
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 3 cut(s) 619, 666, 1001
Bse1I ACTGG 3 cut(s) 329, 521, 900
Bse3DI GCAATG 1 cut(s) 930
Bse8I GATNNNNATC 1 cut(s) 1399
BseBI CCWGG 1 cut(s) 1350
BseCI ATCGAT 1 cut(s) 1403
BseDI CCNNGG 3 cut(s) 721, 995, 1094
BseGI GGATG 3 cut(s) 219, 315, 1012
BseJI GATNNNNATC 1 cut(s) 1399
BseLI CCNNNNNNNGG 3 cut(s) 619, 666, 1001
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 2 cut(s) 70, 168
BseNI ACTGG 3 cut(s) 329, 521, 900
BseRI GAGGAG 5 cut(s) 101, 142, 953, 1199, 1359
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 1 cut(s) 473
BshFI GGCC 1 cut(s) 769
BshNI GGYRCC 3 cut(s) 1090, 1099, 1189
BshVI ATCGAT 1 cut(s) 1403
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 705
BsiWI CGTACG 1 cut(s) 474
BslFI GGGAC 2 cut(s) 57, 1396
BslI CCNNNNNNNGG 3 cut(s) 619, 666, 1001
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 57, 1396
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 1 cut(s) 769
Bsp1286I GDGCHC 2 cut(s) 102, 895
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 3 cut(s) 790, 1400, 1404
BspACI CCGC 3 cut(s) 899, 1019, 1234
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 2 cut(s) 69, 167
BspDI ATCGAT 1 cut(s) 1403
BspHI TCATGA 2 cut(s) 732, 793
BspLI GGNNCC 5 cut(s) 45, 770, 1092, 1101, 1191
BspPI GGATC 2 cut(s) 1399, 1408
BspT107I GGYRCC 3 cut(s) 1090, 1099, 1189
BsrDI GCAATG 1 cut(s) 930
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 3 cut(s) 329, 521, 900
BssECI CCNNGG 3 cut(s) 721, 995, 1094
BssMI GATC 3 cut(s) 790, 1400, 1404
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 1094
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 1350
Bst4CI ACNGT 2 cut(s) 208, 299
Bst6I CTCTTC 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 5 cut(s) 749, 767, 921, 1021, 1310
BstDEI CTNAG 3 cut(s) 39, 56, 154
BstDSI CCRYGG 1 cut(s) 721
BstF5I GGATG 3 cut(s) 219, 315, 1012
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 3 cut(s) 793, 1403, 1407
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 3 cut(s) 790, 1400, 1404
BstMWI GCNNNNNNNGC 3 cut(s) 757, 766, 925
BstNI CCWGG 1 cut(s) 1350
BstNSI RCATGY 2 cut(s) 751, 1420
BstSCI CCNGG 1 cut(s) 1348
BstSLI GKGCMC 1 cut(s) 895
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 1087
Bsu15I ATCGAT 1 cut(s) 1403
BsuRI GGCC 1 cut(s) 769
BsuTUI ATCGAT 1 cut(s) 1403
BtgI CCRYGG 1 cut(s) 721
BtsCI GGATG 3 cut(s) 219, 315, 1012
BtsIMutI CAGTG 4 cut(s) 304, 514, 642, 893
Cac8I GCNNGC 5 cut(s) 749, 767, 921, 1021, 1310
CciI TCATGA 2 cut(s) 732, 793
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 44, 768
ClaI ATCGAT 1 cut(s) 1403
CseI GACGC 1 cut(s) 900
Csp6I GTAC 3 cut(s) 475, 979, 1100
CviQI GTAC 3 cut(s) 475, 979, 1100
DdeI CTNAG 3 cut(s) 39, 56, 154
DpnI GATC 3 cut(s) 792, 1402, 1406
DpnII GATC 3 cut(s) 790, 1400, 1404
DrdI GACNNNNNNGTC 1 cut(s) 51
DseDI GACNNNNNNGTC 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 1094
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 1348
EcoT14I CCWWGG 1 cut(s) 1094
EcoT22I ATGCAT 4 cut(s) 221, 930, 952, 1246
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 1094
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 1190, 1222
FaqI GGGAC 2 cut(s) 57, 1396
FauI CCCGC 1 cut(s) 1012
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 3 cut(s) 206, 302, 1019
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 3 cut(s) 1095, 1326, 1480
GlaI GCGC 1 cut(s) 658
GluI GCNGC 1 cut(s) 462
GsuI CTGGAG 3 cut(s) 287, 989, 1424
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 1 cut(s) 769
HapII CCGG 1 cut(s) 705
HgaI GACGC 1 cut(s) 900
HhaI GCGC 1 cut(s) 659
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 1431
HinfI GANTC 3 cut(s) 211, 572, 708
HpaII CCGG 1 cut(s) 705
HphI GGTGA 3 cut(s) 139, 355, 1066
Hpy166II GTNNAC 2 cut(s) 979, 981
Hpy188I TCNGA 6 cut(s) 124, 236, 610, 818, 828, 1294
Hpy188III TCNNGA 6 cut(s) 266, 386, 733, 794, 1373, 1441
Hpy8I GTNNAC 2 cut(s) 979, 981
Hpy99I CGWCG 2 cut(s) 665, 916
HpyAV CCTTC 3 cut(s) 236, 595, 1168
HpyCH4III ACNGT 2 cut(s) 208, 299
HpyCH4IV ACGT 1 cut(s) 1134
HpyCH4V TGCA 7 cut(s) 177, 219, 751, 841, 928, 950, 1244
HpyF10VI GCNNNNNNNGC 3 cut(s) 757, 766, 925
HpyF3I CTNAG 3 cut(s) 39, 56, 154
HpySE526I ACGT 1 cut(s) 1134
HspAI GCGC 1 cut(s) 657
KpnI GGTACC 1 cut(s) 1103
Kzo9I GATC 3 cut(s) 790, 1400, 1404
LmnI GCTCC 3 cut(s) 1144, 1252, 1372
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 228, 1221
MaeI CTAG 3 cut(s) 1095, 1326, 1480
MaeII ACGT 1 cut(s) 1134
MaeIII GTNAC 4 cut(s) 557, 875, 1027, 1387
MalI GATC 3 cut(s) 792, 1402, 1406
MboI GATC 3 cut(s) 790, 1400, 1404
MboII GAAGA 9 cut(s) 92, 187, 190, 223, 497, 532, 800, 1045, 1213
MfeI CAATTG 1 cut(s) 14
MhlI GDGCHC 2 cut(s) 102, 895
MmeI TCCRAC 1 cut(s) 1161
Mph1103I ATGCAT 4 cut(s) 221, 930, 952, 1246
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 6 cut(s) 191, 1128, 1238, 1280, 1340, 1460
MslI CAYNNNNRTG 1 cut(s) 1229
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 1 cut(s) 1350
MunI CAATTG 1 cut(s) 14
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 1 cut(s) 1350
MwoI GCNNNNNNNGC 3 cut(s) 757, 766, 925
NdeII GATC 3 cut(s) 790, 1400, 1404
NlaIV GGNNCC 5 cut(s) 45, 770, 1092, 1101, 1191
NmeAIII GCCGAG 2 cut(s) 723, 1020
NmuCI GTSAC 2 cut(s) 875, 1027
NsiI ATGCAT 4 cut(s) 221, 930, 952, 1246
NspI RCATGY 2 cut(s) 751, 1420
PaeI GCATGC 1 cut(s) 751
PagI TCATGA 2 cut(s) 732, 793
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 3 cut(s) 211, 572, 708
Pfl23II CGTACG 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 1348
PkrI GCNGC 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 44
PshBI ATTAAT 1 cut(s) 1280
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 1348
PspGI CCWGG 1 cut(s) 1348
PspLI CGTACG 1 cut(s) 474
PspN4I GGNNCC 5 cut(s) 45, 770, 1092, 1101, 1191
PspPI GGNCC 2 cut(s) 44, 768
PspPPI RGGWCCY 1 cut(s) 44
RsaI GTAC 3 cut(s) 476, 980, 1101
RsaNI GTAC 3 cut(s) 475, 979, 1100
RseI CAYNNNNRTG 1 cut(s) 1229
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 6 cut(s) 191, 1128, 1238, 1280, 1340, 1460
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 3 cut(s) 790, 1400, 1404
Sau96I GGNCC 2 cut(s) 44, 768
ScrFI CCNGG 1 cut(s) 1350
SduI GDGCHC 2 cut(s) 102, 895
SfaNI GCATC 2 cut(s) 228, 1221
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 1229
SmlI CTYRAG 2 cut(s) 71, 1371
SmoI CTYRAG 2 cut(s) 71, 1371
SphI GCATGC 1 cut(s) 751
SsiI CCGC 3 cut(s) 899, 1019, 1234
SspI AATATT 1 cut(s) 1344
SspMI CTAG 3 cut(s) 1095, 1326, 1480
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 1348
StyI CCWWGG 1 cut(s) 1094
TaaI ACNGT 2 cut(s) 208, 299
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 4 cut(s) 96, 774, 1393, 1403
TatI WGTACW 1 cut(s) 978
TfiI GAWTC 3 cut(s) 211, 572, 708
Tru1I TTAA 6 cut(s) 191, 1128, 1238, 1280, 1340, 1460
Tru9I TTAA 6 cut(s) 191, 1128, 1238, 1280, 1340, 1460
TscAI CASTG 4 cut(s) 304, 514, 649, 900
TseFI GTSAC 2 cut(s) 875, 1027
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 2 cut(s) 875, 1027
TspDTI ATGAA 9 cut(s) 82, 133, 431, 498, 539, 721, 749, 1213, 1258
TspGWI ACGGA 2 cut(s) 196, 738
TspRI CASTG 4 cut(s) 304, 514, 649, 900
VpaK11BI GGWCC 1 cut(s) 44
VspI ATTAAT 1 cut(s) 1280
XapI RAATTY 5 cut(s) 488, 545, 822, 860, 1013
XceI RCATGY 2 cut(s) 751, 1420
XmaJI CCTAGG 1 cut(s) 1094
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 3 cut(s) 1095, 1326, 1480
Zsp2I ATGCAT 4 cut(s) 221, 930, 952, 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.