pycom15g08610

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013421
Physical Location & Seq
Reverse (-)
5468723 .. 5472940
4218 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2541 bp
ATGAGAGACATCAATTCAATACATGACTTTTTACCACAATATCCAATACTTTGGACTTGTTCATCTCTCCCTCTCTTCCCCTGTATCTTCCTCAATGGCTCACCTCTTCAGAGACCTCTCTTCACTGGCCCAATCCAAGAGAGGCATCACCGCCCCCACAACCACCACAGCCACCGCCTCACCCGCCAAACCACTCAGCATACCCACCAGATCCATTTCCGCAATGGGCACCGATCTCCCATCCCCGCTCGGCCAGCTCTCGGCCCAACTCAGCGACTCGGACCTCCGACTCACCGCCTACGATCGATCTTCGTCGCCGCCTGCCGTACATCCACCGGAAAGGCCTTGACTTTCACACCCTCCTCCGCTGACTCGCCGACCCAACACGCCAACTCCCCCAACGGCTCCCCCGCACTGCAGAGATCGCTCACTTCCACAGCTGCCAGCAAGATGAAGAAGGCATTGGGTCTCAAATCGCCGGGCTCCGGCTCCAAGAAGAGTCCCGGGTCAGCAGGGTCCGGGGCCGGGTCGGGTCCCGGAAAGCCCAGGCGGGCAATGACGGTGGGTGAGCTGATGAGGATCCAAATGGGTATTTCTGAGGCCACTGACTCCAGAGTCCGGAGAGCTCTGCTCAGGATTTCTGCTTCTCAGGTCGGAAGGCGAATTGAGTCAGTGGTGGTGCCACTGGAGCTGTTACAGCAGCTCAAGTCCTCTGATTTCACTGACCAGCAAGAATACGATGCATGGCAGAAAAGAACCCTCAAAATTCTTGAGGCCGGTCTCCTATTGCATCCCCACGTGCCACTTGACAAGTCGAATAATGCTGCACAGAGATTGCGTCAAATTATTAATGGGGCATTGGACAGGCCCTTTGAAACTGGCAGAAATAACGAGACTCTGCAGGTTCTTCGCAATGCTGTTACGGCTCTTGCTTCTAGGTCAGCTGATGGTCTCTATGATACATCACATTGGGCAGATGGGTTGCCATTGAATCTCCGGCTTTATGAGAGACTTCTAGAAGCCTGCTTTGATCGTCACCACGAAACATCTATAATAGAGGAAGTTGACGAGCTAATGGAGCATATTAAGAAGACCTGGTCAATCCTTGGAATGAACCAGATGCTCCATAACCTTTGCTTTACTTGGGTTTTATTTCATCGTTTTGTTGCAACTGGTCAAGTAGAACTGGACCTCTTGTATGCTGCTGATAGTCAGCTAGCTGAGGTTGCTAAAGACGCAAAGGCAACAAAGGATTCCGAGTACTGCAAGATCTTGAGTTCGACATTGACTTCAATATTGGGATGGGCAGAAAAGAGACTCCTTGCGTATCATGACACTTTTGATAGCAGTAACATTGATGCTATGCAGGCCATTGTTTCTCTGGGGGTAGTAGCAGCTAAGATTTTAGTTGAAGATATATCAAATGAGTACCGCAGGAGGAGGAAAAATGAAGTTGATGTTGCCCGTAGCAGGATTGACACCTACATCAGATCATCCTTGCGCACTGCTTTTGCTCAGAGAATGGAAAAGGCCGATTCAAGCAGGAGAGCATCAAGACACCAGCCAAATCCCCTTCCTGTTCTGGCCATCCTTGCAAAGGACGTTGGTGAGCTGGCAGTTAAGGAGAAAGAGGTGTTCAGTCCAATATTGAAGAGATGGCATCCTTTTGCTGCTGGGGTGGCTGTGGCAACCCTTCACGCTTGCTATGCGAATGAGATTAAACAATTCATATCGGGTATAGCGGAGTTGAACCCAGATGCTGTACAGGTTTTGAGAGCTGCTGATAAGCTGGAGAAAGATCTTGTGCTGATAGCAGTTTTAGACTCCGTAGATAGTGATGATGGTGGCAAGGCAATAATTCGAGAGATGCCTCCTTACGAGGCTGAAACTGCAATTGCGAATCTGGTAAAAGTGTGGATTAAGACAAGAGTGGACAGATTGAAAGAATGGATTGATAGGAATCTACGGCAAGAGGTATGGAACCCACAAGTAAACGAAGATGGATATGCCCCATCTGCTGTTGAAGTTTTGCGAATCCTTGATGAAACTCTGGAAGCATTCTTTCAGCTGCCAATACCAATGCATCCTGCATTGCTTCCTGACTTGATGACTGGCCTTGATAGATGTCTTCAGTATTACGTAACCAAGGCAAAATCTGGATGTGGATCTCGCAATACGTTTGTTCCCACTATGCCGGCATTGACCAGATGTACCATGGGATCAAAGTTCCAAGGCTTTGGCAAAAAGAAAGAAAAATCACCAGTTCCTCAAAAGAGGAACTCTCAGGTTGCAACACTCAATGGGGATAACTCCTTTGGGATACCTCAGATGTGTGCTCGTATAAATACATTGCAAAGAATCCGGAGTGAGTTGGAGGTTTTGGAGAAACGGATTATTACTCATCTCAGAAATTCAGAATCTGCTAATGTAGAAGACTTTTCGAATGGACTGGGGAAAAAGTTTGAACTCACGCCAGCAGCTTGTGTGGAAGCAATCCAACAGCTTTGTGAGGCAGTGGCTTATAAAATCGATCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

847

Amino Acids

94.33

Weight (kDa)

9.27

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 632 - 843 4e-74 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25800
fragaria_vesca FvH4_2g35590 FvH4_2g35590
malus_domestica MD08G1113600.v1.1 MD15G1093300.v1.1
prunus_persica Prupe.1G448100_v2.0.a1
pyrus_communis pycom08g09420 pycom15g08610
rosa_chinensis RchiOBHm_Chr6g0306221
rosa_laevigata RLG00000010812
rosa_multiflora Rmu_sc0003594.1_g000002
rosa_roxburghii Rroxscaffold_7G00161920
rosa_rugosa Rorug06G0348100
rosa_samantha Rh6AG460700 Rh6BG440700 Rh6CG474700 Rh6DG461000
rosa_wichuraiana Rw6G040130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2523
AasI GACNNNNNNGTC 1 cut(s) 614
Acc16I TGCGCA 1 cut(s) 1502
Acc36I ACCTGC 1 cut(s) 892
AccB1I GGYRCC 2 cut(s) 228, 679
AccBSI CCGCTC 1 cut(s) 248
AccIII TCCGGA 2 cut(s) 618, 2361
AclWI GGATC 5 cut(s) 205, 574, 587, 2173, 2227
AcoI YGGCCR 2 cut(s) 251, 1584
AcsI RAATTY 2 cut(s) 765, 2410
AcuI CTGAAG 2 cut(s) 92, 2114
AcvI CACGTG 1 cut(s) 799
AfaI GTAC 5 cut(s) 328, 1262, 1430, 1764, 2212
AfiI CCNNNNNNNGG 6 cut(s) 260, 485, 525, 618, 1470, 1597
AjnI CCWGG 2 cut(s) 545, 1094
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
Alw21I GWGCWC 2 cut(s) 628, 2338
Alw26I GTCTC 7 cut(s) 106, 473, 785, 887, 956, 1003, 1309
AlwI GGATC 5 cut(s) 205, 574, 587, 2173, 2227
AlwNI CAGNNNCTG 2 cut(s) 1760, 2420
Ama87I CYCGRG 1 cut(s) 503
Aor13HI TCCGGA 2 cut(s) 618, 2361
ApeKI GCWGC 9 cut(s) 440, 700, 824, 1202, 1394, 1670, 1778, 2068, 2477
ApoI RAATTY 2 cut(s) 765, 2410
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AseI ATTAAT 1 cut(s) 849
AspLEI GCGC 1 cut(s) 1503
AspS9I GGNCC 8 cut(s) 128, 263, 281, 516, 522, 533, 867, 1189
AsuC2I CCSGG 6 cut(s) 480, 504, 505, 520, 526, 537
AsuHPI GGTGA 8 cut(s) 93, 140, 172, 284, 578, 1028, 1619, 2250
AsuII TTCGAA 1 cut(s) 2441
AsuNHI GCTAGC 1 cut(s) 1216
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 4 cut(s) 281, 516, 533, 1189
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 1586
BamHI GGATCC 1 cut(s) 579
BanI GGYRCC 2 cut(s) 228, 679
BanII GRGCYC 2 cut(s) 485, 628
BbrPI CACGTG 1 cut(s) 799
BbsI GAAGAC 3 cut(s) 1097, 2120, 2439
Bbv12I GWGCWC 2 cut(s) 628, 2338
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 9 cut(s) 427, 712, 811, 1189, 1406, 1657, 1765, 2055, 2489
BccI CCATC 9 cut(s) 248, 941, 971, 1296, 1595, 1650, 1835, 1994, 2020
BceAI ACGGC 4 cut(s) 309, 418, 939, 1982
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 2 cut(s) 547, 1096
BciVI GTATCC 1 cut(s) 2313
BcnI CCSGG 6 cut(s) 480, 504, 505, 520, 526, 537
BcoDI GTCTC 7 cut(s) 106, 473, 785, 887, 956, 1003, 1309
BfaI CTAG 3 cut(s) 936, 1016, 1217
BfmI CTRYAG 2 cut(s) 416, 899
BfuAI ACCTGC 1 cut(s) 892
BfuI GTATCC 1 cut(s) 2313
BglII AGATCT 2 cut(s) 1269, 1798
BmcAI AGTACT 1 cut(s) 1262
Bme1390I CCNGG 8 cut(s) 480, 504, 505, 520, 526, 537, 547, 1096
Bme18I GGWCC 4 cut(s) 281, 516, 533, 1189
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 8 cut(s) 128, 263, 281, 516, 522, 533, 867, 1189
BmrFI CCNGG 8 cut(s) 480, 504, 505, 520, 526, 537, 547, 1096
BmrI ACTGGG 1 cut(s) 2459
BmtI GCTAGC 1 cut(s) 1220
BmuI ACTGGG 1 cut(s) 2459
BpiI GAAGAC 3 cut(s) 1097, 2120, 2439
BplI GAGNNNNNCTC 4 cut(s) 615, 647, 2266, 2298
BpmI CTGGAG 3 cut(s) 595, 707, 1811
Bpu10I CCTNAGC 2 cut(s) 632, 1221
Bpu14I TTCGAA 1 cut(s) 2441
BpuEI CTTGAG 3 cut(s) 689, 791, 1294
BpuMI CCSGG 6 cut(s) 480, 504, 505, 520, 526, 537
Bsa29I ATCGAT 2 cut(s) 305, 2529
BsaAI YACGTR 2 cut(s) 799, 2140
BsaBI GATNNNNATC 3 cut(s) 578, 1959, 2164
BsaI GGTCTC 4 cut(s) 106, 473, 785, 956
BsaJI CCNNGG 7 cut(s) 503, 519, 545, 1105, 2145, 2214, 2230
BsaWI WCCGGW 3 cut(s) 335, 618, 2361
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 6 cut(s) 260, 485, 525, 618, 1470, 1597
Bse118I RCCGGY 2 cut(s) 776, 2194
Bse1I ACTGG 8 cut(s) 130, 690, 883, 1177, 1191, 2116, 2261, 2454
Bse3DI GCAATG 5 cut(s) 229, 561, 919, 2090, 2348
Bse8I GATNNNNATC 3 cut(s) 578, 1959, 2164
BseAI TCCGGA 2 cut(s) 618, 2361
BseBI CCWGG 2 cut(s) 547, 1096
BseCI ATCGAT 2 cut(s) 305, 2529
BseDI CCNNGG 7 cut(s) 503, 519, 545, 1105, 2145, 2214, 2230
BseGI GGATG 9 cut(s) 240, 329, 790, 1307, 1493, 1587, 1660, 2083, 2165
BseJI GATNNNNATC 3 cut(s) 578, 1959, 2164
BseLI CCNNNNNNNGG 6 cut(s) 260, 485, 525, 618, 1470, 1597
BseMI GCAATG 5 cut(s) 229, 561, 919, 2090, 2348
BseNI ACTGG 8 cut(s) 130, 690, 883, 1177, 1191, 2116, 2261, 2454
BseRI GAGGAG 2 cut(s) 352, 1453
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 9 cut(s) 427, 712, 811, 1189, 1406, 1657, 1765, 2055, 2489
BseYI CCCAGC 1 cut(s) 1673
BsgI GTGCAG 1 cut(s) 810
Bsh1285I CGRYCG 1 cut(s) 305
BshNI GGYRCC 2 cut(s) 228, 679
BshVI ATCGAT 2 cut(s) 305, 2529
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 628, 2338
BsiHKCI CYCGRG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 486, 519
BslI CCNNNNNNNGG 6 cut(s) 260, 485, 525, 618, 1470, 1597
BsmAI GTCTC 7 cut(s) 106, 473, 785, 887, 956, 1003, 1309
BsmFI GGGAC 2 cut(s) 486, 519
BsmI GAATGC 1 cut(s) 2057
Bso31I GGTCTC 4 cut(s) 106, 473, 785, 956
BsoBI CYCGRG 1 cut(s) 503
Bsp119I TTCGAA 1 cut(s) 2441
Bsp1286I GDGCHC 4 cut(s) 231, 485, 628, 2338
Bsp13I TCCGGA 2 cut(s) 618, 2361
Bsp1407I TGTACA 1 cut(s) 1762
Bsp19I CCATGG 1 cut(s) 2214
BspDI ATCGAT 2 cut(s) 305, 2529
BspEI TCCGGA 2 cut(s) 618, 2361
BspHI TCATGA 1 cut(s) 1330
BspMAI CTGCAG 2 cut(s) 420, 903
BspMI ACCTGC 1 cut(s) 892
BspOI GCTAGC 1 cut(s) 1220
BspPI GGATC 5 cut(s) 205, 574, 587, 2173, 2227
BspT104I TTCGAA 1 cut(s) 2441
BspT107I GGYRCC 2 cut(s) 228, 679
BspTNI GGTCTC 4 cut(s) 106, 473, 785, 956
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 5 cut(s) 229, 561, 919, 2090, 2348
BsrFI RCCGGY 2 cut(s) 776, 2194
BsrGI TGTACA 1 cut(s) 1762
BsrI ACTGG 8 cut(s) 130, 690, 883, 1177, 1191, 2116, 2261, 2454
BssAI RCCGGY 2 cut(s) 776, 2194
BssECI CCNNGG 7 cut(s) 503, 519, 545, 1105, 2145, 2214, 2230
BssT1I CCWWGG 4 cut(s) 1105, 2145, 2214, 2230
Bst2UI CCWGG 2 cut(s) 547, 1096
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 5 cut(s) 80, 111, 125, 491, 1646
BstAUI TGTACA 1 cut(s) 1762
BstBAI YACGTR 2 cut(s) 799, 2140
BstBI TTCGAA 1 cut(s) 2441
BstDSI CCRYGG 1 cut(s) 2214
BstENI CCTNNNNNAGG 1 cut(s) 1595
BstF5I GGATG 9 cut(s) 240, 329, 790, 1307, 1493, 1587, 1660, 2083, 2165
BstHHI GCGC 1 cut(s) 1503
BstMAI GTCTC 7 cut(s) 106, 473, 785, 887, 956, 1003, 1309
BstMCI CGRYCG 1 cut(s) 305
BstNI CCWGG 2 cut(s) 547, 1096
BstSCI CCNGG 8 cut(s) 478, 502, 503, 518, 524, 535, 545, 1094
BstSFI CTRYAG 2 cut(s) 416, 899
BstSLI GKGCMC 1 cut(s) 231
BstSNI TACGTA 1 cut(s) 2140
BstV1I GCAGC 9 cut(s) 427, 712, 811, 1189, 1406, 1657, 1765, 2055, 2489
BstV2I GAAGAC 3 cut(s) 1097, 2120, 2439
BstX2I RGATCY 5 cut(s) 210, 579, 1269, 1798, 2165
BstXI CCANNNNNNTGG 2 cut(s) 51, 2237
BstYI RGATCY 5 cut(s) 210, 579, 1269, 1798, 2165
Bsu15I ATCGAT 2 cut(s) 305, 2529
BsuI GTATCC 1 cut(s) 2313
BsuTUI ATCGAT 2 cut(s) 305, 2529
BtgI CCRYGG 1 cut(s) 2214
BtsCI GGATG 9 cut(s) 240, 329, 790, 1307, 1493, 1587, 1660, 2083, 2165
BtsI GCAGTG 3 cut(s) 413, 1503, 2520
BtsIMutI CAGTG 8 cut(s) 123, 413, 603, 678, 683, 720, 1503, 2520
BveI ACCTGC 1 cut(s) 892
CaiI CAGNNNCTG 2 cut(s) 1760, 2420
CciI TCATGA 1 cut(s) 1330
CfoI GCGC 1 cut(s) 1503
Cfr10I RCCGGY 2 cut(s) 776, 2194
Cfr13I GGNCC 8 cut(s) 128, 263, 281, 516, 522, 533, 867, 1189
Cfr9I CCCGGG 1 cut(s) 503
ClaI ATCGAT 2 cut(s) 305, 2529
CseI GACGC 2 cut(s) 827, 1244
CsiI ACCWGGT 1 cut(s) 1094
Csp6I GTAC 5 cut(s) 327, 1261, 1429, 1763, 2211
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviAII CATG 5 cut(s) 23, 744, 1331, 2215, 2538
CviQI GTAC 5 cut(s) 327, 1261, 1429, 1763, 2211
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 2 cut(s) 251, 1584
Eam1104I CTCTTC 5 cut(s) 80, 111, 125, 491, 1646
EarI CTCTTC 5 cut(s) 80, 111, 125, 491, 1646
Ecl136II GAGCTC 1 cut(s) 626
Eco105I TACGTA 1 cut(s) 2140
Eco130I CCWWGG 4 cut(s) 1105, 2145, 2214, 2230
Eco147I AGGCCT 1 cut(s) 344
Eco24I GRGCYC 2 cut(s) 485, 628
Eco31I GGTCTC 4 cut(s) 106, 473, 785, 956
Eco47I GGWCC 4 cut(s) 281, 516, 533, 1189
Eco53kI GAGCTC 1 cut(s) 626
Eco57I CTGAAG 2 cut(s) 92, 2114
Eco72I CACGTG 1 cut(s) 799
Eco88I CYCGRG 1 cut(s) 503
EcoICRI GAGCTC 1 cut(s) 626
EcoNI CCTNNNNNAGG 1 cut(s) 1595
EcoO109I RGGNCCY 2 cut(s) 533, 867
EcoRII CCWGG 2 cut(s) 545, 1094
EcoT14I CCWWGG 4 cut(s) 1105, 2145, 2214, 2230
EcoT22I ATGCAT 2 cut(s) 745, 2085
EcoT38I GRGCYC 2 cut(s) 485, 628
ErhI CCWWGG 4 cut(s) 1105, 2145, 2214, 2230
FaeI CATG 5 cut(s) 26, 747, 1334, 2218, 2541
FaqI GGGAC 2 cut(s) 486, 519
FatI CATG 5 cut(s) 22, 743, 1330, 2214, 2537
FauI CCCGC 4 cut(s) 191, 253, 418, 543
FokI GGATG 9 cut(s) 227, 316, 777, 1314, 1480, 1574, 1647, 2070, 2172
FriOI GRGCYC 2 cut(s) 485, 628
FspBI CTAG 3 cut(s) 936, 1016, 1217
FspI TGCGCA 1 cut(s) 1502
GlaI GCGC 1 cut(s) 1502
GsaI CCCAGC 1 cut(s) 1677
GsuI CTGGAG 3 cut(s) 595, 707, 1811
HgaI GACGC 2 cut(s) 827, 1244
HhaI GCGC 1 cut(s) 1503
Hin1II CATG 5 cut(s) 26, 747, 1334, 2218, 2541
Hin6I GCGC 1 cut(s) 1501
HinP1I GCGC 1 cut(s) 1501
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HphI GGTGA 8 cut(s) 93, 140, 172, 284, 578, 1028, 1619, 2250
Hpy166II GTNNAC 3 cut(s) 1066, 1933, 1993
Hpy8I GTNNAC 3 cut(s) 1066, 1933, 1993
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 4 cut(s) 451, 651, 1583, 1703
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 4 cut(s) 798, 1602, 2139, 2177
HpySE526I ACGT 4 cut(s) 798, 1602, 2139, 2177
Hsp92II CATG 5 cut(s) 26, 747, 1334, 2218, 2541
HspAI GCGC 1 cut(s) 1501
KflI GGGWCCC 1 cut(s) 533
Kpn2I TCCGGA 2 cut(s) 618, 2361
KroI GCCGGC 1 cut(s) 2194
KroNI GCCGGC 1 cut(s) 2196
LmnI GCTCC 6 cut(s) 410, 488, 494, 688, 1078, 1128
Lsp1109I GCAGC 9 cut(s) 427, 712, 811, 1189, 1406, 1657, 1765, 2055, 2489
MabI ACCWGGT 1 cut(s) 1094
MaeI CTAG 3 cut(s) 936, 1016, 1217
MaeII ACGT 4 cut(s) 798, 1602, 2139, 2177
MaeIII GTNAC 5 cut(s) 693, 919, 1034, 1349, 2140
MbiI CCGCTC 1 cut(s) 248
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 5 cut(s) 210, 579, 1269, 1798, 2165
MhlI GDGCHC 4 cut(s) 231, 485, 628, 2338
MlsI TGGCCA 1 cut(s) 1586
MluCI AATT 8 cut(s) 13, 663, 765, 843, 1724, 1857, 1893, 2410
MluNI TGGCCA 1 cut(s) 1586
MmeI TCCRAC 4 cut(s) 311, 634, 2352, 2521
Mox20I TGGCCA 1 cut(s) 1586
Mph1103I ATGCAT 2 cut(s) 745, 2085
MroI TCCGGA 2 cut(s) 618, 2361
MroNI GCCGGC 1 cut(s) 2194
MscI TGGCCA 1 cut(s) 1586
MseI TTAA 5 cut(s) 849, 1086, 1620, 1719, 1920
Msp20I TGGCCA 1 cut(s) 1586
MspA1I CMGCKG 4 cut(s) 368, 440, 944, 2068
MspR9I CCNGG 8 cut(s) 480, 504, 505, 520, 526, 537, 547, 1096
MunI CAATTG 1 cut(s) 1893
Mva1269I GAATGC 1 cut(s) 2057
MvaI CCWGG 2 cut(s) 547, 1096
NaeI GCCGGC 1 cut(s) 2196
NciI CCSGG 6 cut(s) 480, 504, 505, 520, 526, 537
NcoI CCATGG 1 cut(s) 2214
NgoMIV GCCGGC 1 cut(s) 2194
NheI GCTAGC 1 cut(s) 1216
NlaIII CATG 5 cut(s) 26, 747, 1334, 2218, 2541
NmeAIII GCCGAG 2 cut(s) 229, 240
NmuCI GTSAC 1 cut(s) 1034
NsbI TGCGCA 1 cut(s) 1502
NsiI ATGCAT 2 cut(s) 745, 2085
NspV TTCGAA 1 cut(s) 2441
PagI TCATGA 1 cut(s) 1330
PceI AGGCCT 1 cut(s) 344
PctI GAATGC 1 cut(s) 2057
PdiI GCCGGC 1 cut(s) 2196
PfeI GAWTC 8 cut(s) 991, 1253, 1535, 1900, 1960, 2034, 2358, 2417
PflFI GACNNNGTC 1 cut(s) 1096
PfoI TCCNGGA 1 cut(s) 535
Ple19I CGATCG 1 cut(s) 305
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
Ppu21I YACGTR 2 cut(s) 799, 2140
PpuMI RGGWCCY 1 cut(s) 533
PshBI ATTAAT 1 cut(s) 849
PsiI TTATAA 1 cut(s) 2523
Psp124BI GAGCTC 1 cut(s) 628
Psp5II RGGWCCY 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 545, 1094
PspCI CACGTG 1 cut(s) 799
PspFI CCCAGC 1 cut(s) 1673
PspGI CCWGG 2 cut(s) 545, 1094
PspPI GGNCC 8 cut(s) 128, 263, 281, 516, 522, 533, 867, 1189
PspPPI RGGWCCY 1 cut(s) 533
PstI CTGCAG 2 cut(s) 420, 903
PstNI CAGNNNCTG 2 cut(s) 1760, 2420
PsuI RGATCY 5 cut(s) 210, 579, 1269, 1798, 2165
PsyI GACNNNGTC 1 cut(s) 1096
PvuI CGATCG 1 cut(s) 305
PvuII CAGCTG 3 cut(s) 440, 944, 2068
RsaI GTAC 5 cut(s) 328, 1262, 1430, 1764, 2212
RsaNI GTAC 5 cut(s) 327, 1261, 1429, 1763, 2211
SacI GAGCTC 1 cut(s) 628
SaqAI TTAA 5 cut(s) 849, 1086, 1620, 1719, 1920
Sau96I GGNCC 8 cut(s) 128, 263, 281, 516, 522, 533, 867, 1189
ScaI AGTACT 1 cut(s) 1262
ScrFI CCNGG 8 cut(s) 480, 504, 505, 520, 526, 537, 547, 1096
SduI GDGCHC 4 cut(s) 231, 485, 628, 2338
SexAI ACCWGGT 1 cut(s) 1094
SfcI CTRYAG 2 cut(s) 416, 899
SfuI TTCGAA 1 cut(s) 2441
SinI GGWCC 4 cut(s) 281, 516, 533, 1189
SmaI CCCGGG 1 cut(s) 505
SmlI CTYRAG 3 cut(s) 704, 770, 1273
SmoI CTYRAG 3 cut(s) 704, 770, 1273
SnaBI TACGTA 1 cut(s) 2140
Sse9I AATT 8 cut(s) 13, 663, 765, 843, 1724, 1857, 1893, 2410
SseBI AGGCCT 1 cut(s) 344
SspI AATATT 2 cut(s) 1296, 1647
SspMI CTAG 3 cut(s) 936, 1016, 1217
SstI GAGCTC 1 cut(s) 628
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 8 cut(s) 478, 502, 503, 518, 524, 535, 545, 1094
StyI CCWWGG 4 cut(s) 1105, 2145, 2214, 2230
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 4 cut(s) 801, 1605, 2142, 2180
TaqI TCGA 6 cut(s) 305, 815, 1280, 1861, 2441, 2529
TasI AATT 8 cut(s) 13, 663, 765, 843, 1724, 1857, 1893, 2410
TatI WGTACW 2 cut(s) 1260, 1762
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 8 cut(s) 991, 1253, 1535, 1900, 1960, 2034, 2358, 2417
Tru1I TTAA 5 cut(s) 849, 1086, 1620, 1719, 1920
Tru9I TTAA 5 cut(s) 849, 1086, 1620, 1719, 1920
TscAI CASTG 8 cut(s) 130, 420, 610, 678, 690, 727, 1510, 2520
TseFI GTSAC 1 cut(s) 1034
TseI GCWGC 9 cut(s) 440, 700, 824, 1202, 1394, 1670, 1778, 2068, 2477
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 7 cut(s) 51, 467, 1127, 1145, 1464, 1717, 2058
TspGWI ACGGA 2 cut(s) 1816, 2404
TspMI CCCGGG 1 cut(s) 503
TspRI CASTG 8 cut(s) 130, 420, 610, 678, 690, 727, 1510, 2520
Tth111I GACNNNGTC 1 cut(s) 1096
VpaK11BI GGWCC 4 cut(s) 281, 516, 533, 1189
VspI ATTAAT 1 cut(s) 849
XagI CCTNNNNNAGG 1 cut(s) 1595
XapI RAATTY 2 cut(s) 765, 2410
XbaI TCTAGA 1 cut(s) 1015
XcmI CCANNNNNNNNNTGG 3 cut(s) 221, 1378, 2212
XmaI CCCGGG 1 cut(s) 503
XspI CTAG 3 cut(s) 936, 1016, 1217
ZrmI AGTACT 1 cut(s) 1262
Zsp2I ATGCAT 2 cut(s) 745, 2085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.