Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0007701

Physical Location & Seq

Genomic Coordinates Reverse (-)

8409293 .. 8419077

Gene Structure 9785 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2502 bp

ATGAAGCTGCTGCCGCCATTGCTGCTGCTTTTGCTGTTACCGTGTTGGGCAATCGCGCTATTGGCAACGGCTGCGGCGCGCAATGTGAGCTACGACAGCCGGTCTCTGATCATCGATGGCCAGCGGAAGCTCCTCATCTCTGCTGCCATTCACTACCCTCGCAGCGTCCCTGAGATGTGGCCGAAGCTAGTTCAAACGGCGAAAGAAGGAGGGGTGGATGTGATTGAGACGTATGTGTTTTGGAATGGCCATGAACCATCTCCAGGCAATGCTGGGATGCATTTGATTCTTCGAATTGGCCCATTTGTTGCAGCAGAATGGTACTTTGGAGGGATACCTGTTTGGTTGCATTATGTGCCTGGCACTGTGTTTCGGACTGAAAATAAGCCTTTTAAGTATCACATGCAGAAGTTCACAACATTCATAGTTGACCTAATGAAACAGGAGAAGTTTTTTGCATCGCAAGGGGGTCCTATTATCTTGGCCCAGGTAGAAAATGAATATGGATACTATGAAAAGGATTACGGAGAAGGGGGTAAACAATATGCTATGTGGGCTGCTAGTATGGCTGTTTCTCAGAATATAGGTGTCCCCTGGATAATGTGCCAACAATTTGATGCTCCAGAGTCTGTGATAAATACTTGTAACTCCTTCTACTGTGACCAGTTTACGCCTATCTATCAGAACAAACCTAAAATTTGGACAGAGAATTGGCCTGGCTGGTTTAAAACATTTGGGGGCTGGAATCCTCACCGGCCAGCTGAAGATATTGCTTTTTCTGTAGCTCGCTTTTTCCAAAAAGGCGGAAGCGTACACAATTACTATATGTATCATGGAGGAACAAATTTTGGTCGAACTTCAGGTGGACCATTCATTACCACAAGTTATGACTATGAGGCACCAATTGATGAATATGGATTACCTAGGCTTCCAAAATGGGGGCACCTTAAGCAACTTCACAGAGCCATCAAGTTATGTGAGCATATTATGCTCAACAGTCAGCCAACTAATGTATCACTGGGTCCTTCCCTAGAGGCTGATGTCTTCACCAACTCTTCTGGAGCATGTGCTGCCTTTATAGCAAACATGGATGACAAAAATGACAAGACAGTAGAATTTCGAAACATGTCATATCATCTTCCAGCATGGTCGGTTAGCATACTGCCAGACTGCAAGAATGTAGTGTTTAATACAGCAAAGGTTGGATCCCAGAGCTCTGTAGTTGAAATGTTACCTGAGAGTTTGCAGCTATCAGTGGGATCAGCAGATAAAAGCTTGAAAGATCTGAAATGGGATGTATTTGTGGAGAAAGCTGGGATTTGGGGTGAAGCGGACTTTGTAAAAAGTGGCTTGGTAGATCACATTAACACAACAAAATTTACGACAGACTATCTTTGGTATACAACAAGCATATTGGTCGGTGAAAATGAAGAGTTCTTAAAAAAAGGAAGCTCGCCAGTTCTTCTAATTGAATCAAAGGGTCATGCTGTCCATGCTTTTGTGAATCAAGAGCTCCAAGCTAGTGCAGCTGGAAATGGCACGCACTTCCCCTTCAAATTAAAAACTCCCATCTCTCTGAAGGAGGGGAAGAATGATATTGCTCTACTAAGCATGACAGTTGGTCTGCAGAACGCAGGATCATTTTATGAATGGGTAGGGGCAGGGCTAACTAGTGTCAAGATTCAGGGATTCAACAATGGGACAATAGATTTGTCAGCGTATAATTGGACCTACAAGATTGGATTGGAAGGGGAGCATCAAGGTCTTGACAAGGAAGAAGGTTTCAGTAATGTAAATTGGATTTCAGCCTCAGAACCTCCAAAGGAACAGCCTTTGACATGGTATAAGGTTATTGTGGACCCTCCACCGGGTGATGATCCCGTTGGTTTGGATATGATTCACATGGGAAAAGGTCTAGCTTGGTTAAATGGAGAAGAGATTGGAAGATACTGGCCTAGGAAGGGCCCTTTACATGGATGTGTTAAAGAATGCAATTACAGAGGCAAATTCGATCCAGACAAATGCAATACAGGGTGTGGAGAACCAACGCAAAGATGGTACCATGTTCCACGGTCTTGGTTCAAGCAATCTGGAAACGTACTGGTGATATTCGAGGAGAAAGGTGGAGATCCATCCAAAATTGAGTTCTCAAGGCGGAAAATCACCGGTGTGTGCGCTCTTGTAGCAGAGAATTACCCCTCTATTGACCTTGAATCTTGGAATGATGGAAGTGGAAGTAACAAAACCGTTGCAACCATCCATTTAGGTTGCCCCGAGGATACACATATTTCAAGTGTAAAATTTGCCAGCTTCGGGAATCCTACAGGCGCCTGTGGGTCCTATACACAGGGCAACTGCCATGACCCTAACTCCATTTCTGTGGTCGAAAAGGTCTGCTTAAATAAGAACCAATGCGACGTAGAATTAACAGAAGAAAATTTTAATAAGGGTTCGTGTCTGAGTGAACCAAAGAAGCTTGCAGTTCAAGTACAGTGCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0009505 GO:0009628 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944 GO:0080167

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

834

Amino Acids

92.69

Weight (kDa)

6.05

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 90	5.4e-19	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	90 - 322	2.3e-80	Glycosyl hydrolases family 35
GHD	PF17834	345 - 401	5.2e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	612 - 699	1.1e-20	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	755 - 832	6.5e-17	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63810
fragaria_vesca	FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130
malus_domestica	MD15G1136400.v1.1
prunus_persica	Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1
pyrus_communis	pycom15g12300
rosa_chinensis	RchiOBHm_Chr2g0122791
rosa_laevigata	RLG00000018715
rosa_multiflora	Rmu_sc0008528.1_g000013 Rmu_sc0037008.1_g000001
rosa_roxburghii	Rroxscaffold_2G00121170
rosa_rugosa	Rorug02G0242600 Rorug02G0242700 Rorug02G0242700
rosa_samantha	Rh2AG301300 Rh2BG308700 Rh2CG287400 Rh2DG325500
rosa_wichuraiana	Rw2G023890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2058
AccB1I	GGYRCC	4 cut(s)	898, 942, 2058, 2327
AccI	GTMKAC	1 cut(s)	1400
AccII	CGCG	2 cut(s)	56, 79
AciI	CCGC	6 cut(s)	14, 74, 124, 804, 1331, 2155
AclWI	GGATC	7 cut(s)	1200, 1213, 1267, 1645, 1871, 2006, 2123
AcoI	YGGCCR	4 cut(s)	118, 179, 247, 755
AcsI	RAATTY	7 cut(s)	696, 844, 1115, 1376, 2006, 2300, 2437
AcuI	CTGAAG	3 cut(s)	783, 843, 1598
AcyI	GRCGYC	1 cut(s)	2328
AdeI	CACNNNGTG	1 cut(s)	1871
AfaI	GTAC	5 cut(s)	323, 813, 2060, 2100, 2490
AfiI	CCNNNNNNNGG	7 cut(s)	263, 938, 1867, 1868, 1961, 1973, 2313
AflII	CTTAAG	1 cut(s)	947
AflIII	ACRYGT	1 cut(s)	1125
AgeI	ACCGGT	1 cut(s)	2165
AhdI	GACNNNNNGTC	2 cut(s)	100, 1620
AhlI	ACTAGT	1 cut(s)	1670
AjnI	CCWGG	5 cut(s)	262, 358, 486, 593, 715
AjuI	GAANNNNNNNTTGG	4 cut(s)	309, 341, 2129, 2161
AleI	CACNNNNGTG	1 cut(s)	2168
Alw21I	GWGCWC	2 cut(s)	1217, 1515
Alw26I	GTCTC	2 cut(s)	108, 221
AlwI	GGATC	7 cut(s)	1200, 1213, 1267, 1645, 1871, 2006, 2123
AlwNI	CAGNNNCTG	1 cut(s)	629
Ama87I	CYCGRG	1 cut(s)	2273
AoxI	GGCC	9 cut(s)	118, 179, 247, 298, 483, 713, 755, 1952, 1963
ApaI	GGGCCC	1 cut(s)	1967
ApoI	RAATTY	7 cut(s)	696, 844, 1115, 1376, 2006, 2300, 2437
ArsI	GACNNNNNNTTYG	2 cut(s)	1693, 1725
AsiGI	ACCGGT	1 cut(s)	2165
Asp718I	GGTACC	1 cut(s)	2058
AspA2I	CCTAGG	2 cut(s)	923, 1955
AspLEI	GCGC	5 cut(s)	58, 79, 81, 2177, 2330
AsuC2I	CCSGG	1 cut(s)	1869
AsuHPI	GGTGA	7 cut(s)	743, 1039, 1337, 1433, 1883, 2116, 2155
AsuII	TTCGAA	2 cut(s)	292, 1120
AvaI	CYCGRG	1 cut(s)	2273
AvaII	GGWCC	6 cut(s)	470, 866, 1022, 1728, 1858, 2337
AvrII	CCTAGG	2 cut(s)	923, 1955
BaeGI	GKGCMC	2 cut(s)	945, 1967
BaeI	ACNNNNGTAYC	2 cut(s)	996, 1029
BalI	TGGCCA	2 cut(s)	120, 249
BamHI	GGATCC	1 cut(s)	1205
BanI	GGYRCC	4 cut(s)	898, 942, 2058, 2327
BanII	GRGCYC	3 cut(s)	1217, 1515, 1967
BbsI	GAAGAC	1 cut(s)	1036
Bbv12I	GWGCWC	2 cut(s)	1217, 1515
BccI	CCATC	8 cut(s)	110, 265, 974, 1577, 2049, 2140, 2219, 2264
BceAI	ACGGC	2 cut(s)	84, 213
BcgI	CGANNNNNNTGC	2 cut(s)	1399, 1433
BciT130I	CCWGG	5 cut(s)	264, 360, 488, 595, 717
BciVI	GTATCC	3 cut(s)	327, 500, 2272
BclI	TGATCA	1 cut(s)	108
BcnI	CCSGG	1 cut(s)	1869
BcoDI	GTCTC	2 cut(s)	108, 221
BcuI	ACTAGT	1 cut(s)	1670
BfaI	CTAG	8 cut(s)	188, 561, 924, 1031, 1521, 1671, 1916, 1956
BfmI	CTRYAG	4 cut(s)	780, 1218, 1625, 2322
BfoI	RGCGCY	1 cut(s)	2331
BfrI	CTTAAG	1 cut(s)	947
BfuI	GTATCC	3 cut(s)	327, 500, 2272
BglII	AGATCT	1 cut(s)	1282
BlnI	CCTAGG	2 cut(s)	923, 1955
Bme1390I	CCNGG	6 cut(s)	264, 360, 488, 595, 717, 1869
Bme18I	GGWCC	6 cut(s)	470, 866, 1022, 1728, 1858, 2337
BmeRI	GACNNNNNGTC	2 cut(s)	100, 1620
BmeT110I	CYCGRG	1 cut(s)	2273
BmrFI	CCNGG	6 cut(s)	264, 360, 488, 595, 717, 1869
BmrI	ACTGGG	1 cut(s)	1028
BmsI	GCATC	4 cut(s)	267, 467, 607, 1765
BmuI	ACTGGG	1 cut(s)	1028
BpiI	GAAGAC	1 cut(s)	1036
BpmI	CTGGAG	3 cut(s)	246, 606, 1080
Bpu14I	TTCGAA	2 cut(s)	292, 1120
BpuEI	CTTGAG	1 cut(s)	2134
BpuMI	CCSGG	1 cut(s)	1869
Bsa29I	ATCGAT	1 cut(s)	114
BsaHI	GRCGYC	1 cut(s)	2328
BsaI	GGTCTC	1 cut(s)	108
BsaJI	CCNNGG	6 cut(s)	486, 593, 923, 1955, 2069, 2274
BsaWI	WCCGGW	1 cut(s)	2165
BsaXI	ACNNNNNCTCC	2 cut(s)	2354, 2384
Bsc4I	CCNNNNNNNGG	7 cut(s)	263, 938, 1867, 1868, 1961, 1973, 2313
Bse118I	RCCGGY	3 cut(s)	99, 753, 2165
Bse1I	ACTGG	5 cut(s)	664, 1023, 1457, 1955, 2106
Bse3DI	GCAATG	3 cut(s)	17, 88, 274
BseBI	CCWGG	5 cut(s)	264, 360, 488, 595, 717
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	6 cut(s)	486, 593, 923, 1955, 2069, 2274
BseGI	GGATG	7 cut(s)	223, 282, 1096, 1300, 1982, 2132, 2256
BseLI	CCNNNNNNNGG	7 cut(s)	263, 938, 1867, 1868, 1961, 1973, 2313
BseMI	GCAATG	3 cut(s)	17, 88, 274
BseMII	CTCAG	5 cut(s)	162, 590, 1227, 1824, 2450
BseNI	ACTGG	5 cut(s)	664, 1023, 1457, 1955, 2106
BsePI	GCGCGC	1 cut(s)	77
BseRI	GAGGAG	2 cut(s)	122, 2129
BseSI	GKGCMC	2 cut(s)	945, 1967
BseYI	CCCAGC	2 cut(s)	272, 1313
BsgI	GTGCAG	1 cut(s)	1545
Bsh1236I	CGCG	2 cut(s)	56, 79
BshFI	GGCC	9 cut(s)	120, 181, 249, 300, 485, 715, 757, 1954, 1965
BshNI	GGYRCC	4 cut(s)	898, 942, 2058, 2327
BshTI	ACCGGT	1 cut(s)	2165
BshVI	ATCGAT	1 cut(s)	114
BsiHKAI	GWGCWC	2 cut(s)	1217, 1515
BsiHKCI	CYCGRG	1 cut(s)	2273
BsiSI	CCGG	4 cut(s)	100, 754, 1868, 2166
BslFI	GGGAC	3 cut(s)	152, 575, 1714
BslI	CCNNNNNNNGG	7 cut(s)	263, 938, 1867, 1868, 1961, 1973, 2313
BsmAI	GTCTC	2 cut(s)	108, 221
BsmBI	CGTCTC	1 cut(s)	221
BsmFI	GGGAC	3 cut(s)	152, 575, 1714
BsmI	GAATGC	1 cut(s)	1994
BsnI	GGCC	9 cut(s)	120, 181, 249, 300, 485, 715, 757, 1954, 1965
Bso31I	GGTCTC	1 cut(s)	108
BsoBI	CYCGRG	1 cut(s)	2273
Bsp119I	TTCGAA	2 cut(s)	292, 1120
Bsp120I	GGGCCC	1 cut(s)	1963
Bsp1286I	GDGCHC	4 cut(s)	945, 1217, 1515, 1967
Bsp143I	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
BspACI	CCGC	6 cut(s)	14, 74, 124, 804, 1331, 2155
BspANI	GGCC	9 cut(s)	120, 181, 249, 300, 485, 715, 757, 1954, 1965
BspCNI	CTCAG	5 cut(s)	163, 589, 1228, 1823, 2451
BspDI	ATCGAT	1 cut(s)	114
BspFNI	CGCG	2 cut(s)	56, 79
BspMAI	CTGCAG	1 cut(s)	1629
BspPI	GGATC	7 cut(s)	1200, 1213, 1267, 1645, 1871, 2006, 2123
BspT104I	TTCGAA	2 cut(s)	292, 1120
BspT107I	GGYRCC	4 cut(s)	898, 942, 2058, 2327
BspTI	CTTAAG	1 cut(s)	947
BspTNI	GGTCTC	1 cut(s)	108
BsrDI	GCAATG	3 cut(s)	17, 88, 274
BsrFI	RCCGGY	3 cut(s)	99, 753, 2165
BsrI	ACTGG	5 cut(s)	664, 1023, 1457, 1955, 2106
BssAI	RCCGGY	3 cut(s)	99, 753, 2165
BssECI	CCNNGG	6 cut(s)	486, 593, 923, 1955, 2069, 2274
BssHII	GCGCGC	1 cut(s)	77
BssMI	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
BssNAI	GTATAC	1 cut(s)	1401
BssNI	GRCGYC	1 cut(s)	2328
BssT1I	CCWWGG	2 cut(s)	923, 1955
Bst1107I	GTATAC	1 cut(s)	1401
Bst2UI	CCWGG	5 cut(s)	264, 360, 488, 595, 717
Bst4CI	ACNGT	9 cut(s)	42, 367, 659, 998, 1111, 1618, 2073, 2248, 2493
Bst6I	CTCTTC	3 cut(s)	1060, 1425, 1929
BstACI	GRCGYC	1 cut(s)	2328
BstAFI	CTTAAG	1 cut(s)	947
BstAPI	GCANNNNNTGC	4 cut(s)	71, 355, 988, 1070
BstBI	TTCGAA	2 cut(s)	292, 1120
BstC8I	GCNNGC	8 cut(s)	79, 122, 759, 787, 1454, 1541, 2308, 2478
BstDEI	CTNAG	6 cut(s)	171, 576, 1236, 1607, 1810, 2459
BstDSI	CCRYGG	1 cut(s)	2069
BstF5I	GGATG	7 cut(s)	223, 282, 1096, 1300, 1982, 2132, 2256
BstFNI	CGCG	2 cut(s)	56, 79
BstH2I	RGCGCY	1 cut(s)	2331
BstHHI	GCGC	5 cut(s)	58, 79, 81, 2177, 2330
BstKTI	GATC	9 cut(s)	111, 1208, 1262, 1285, 1360, 1640, 1879, 2014, 2131
BstMAI	GTCTC	2 cut(s)	108, 221
BstMBI	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
BstNI	CCWGG	5 cut(s)	264, 360, 488, 595, 717
BstNSI	RCATGY	3 cut(s)	406, 1068, 1129
BstSCI	CCNGG	6 cut(s)	262, 358, 486, 593, 715, 1867
BstSFI	CTRYAG	4 cut(s)	780, 1218, 1625, 2322
BstSLI	GKGCMC	2 cut(s)	945, 1967
BstUI	CGCG	2 cut(s)	56, 79
BstV2I	GAAGAC	1 cut(s)	1036
BstX2I	RGATCY	3 cut(s)	1205, 1282, 2128
BstXI	CCANNNNNNTGG	2 cut(s)	2076, 2380
BstYI	RGATCY	3 cut(s)	1205, 1282, 2128
BstZ17I	GTATAC	1 cut(s)	1401
Bsu15I	ATCGAT	1 cut(s)	114
BsuI	GTATCC	3 cut(s)	327, 500, 2272
BsuRI	GGCC	9 cut(s)	120, 181, 249, 300, 485, 715, 757, 1954, 1965
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	1 cut(s)	2069
BtgZI	GCGATG	1 cut(s)	444
BtsCI	GGATG	7 cut(s)	223, 282, 1096, 1300, 1982, 2132, 2256
BtsIMutI	CAGTG	4 cut(s)	363, 1016, 1260, 2498
Cac8I	GCNNGC	8 cut(s)	79, 122, 759, 787, 1454, 1541, 2308, 2478
CaiI	CAGNNNCTG	1 cut(s)	629
CfoI	GCGC	5 cut(s)	58, 79, 81, 2177, 2330
Cfr10I	RCCGGY	3 cut(s)	99, 753, 2165
ClaI	ATCGAT	1 cut(s)	114
CseI	GACGC	1 cut(s)	154
Csp6I	GTAC	5 cut(s)	322, 812, 2059, 2099, 2489
CspAI	ACCGGT	1 cut(s)	2165
CviQI	GTAC	5 cut(s)	322, 812, 2059, 2099, 2489
DdeI	CTNAG	6 cut(s)	171, 576, 1236, 1607, 1810, 2459
DinI	GGCGCC	1 cut(s)	2329
DpnI	GATC	9 cut(s)	110, 1207, 1261, 1284, 1359, 1639, 1878, 2013, 2130
DpnII	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
DraI	TTTAAA	1 cut(s)	727
DraIII	CACNNNGTG	1 cut(s)	1871
DriI	GACNNNNNGTC	2 cut(s)	100, 1620
EaeI	YGGCCR	4 cut(s)	118, 179, 247, 755
Eam1104I	CTCTTC	3 cut(s)	1060, 1425, 1929
Eam1105I	GACNNNNNGTC	2 cut(s)	100, 1620
EarI	CTCTTC	3 cut(s)	1060, 1425, 1929
EciI	GGCGGA	2 cut(s)	819, 2170
Ecl136II	GAGCTC	2 cut(s)	1215, 1513
Eco130I	CCWWGG	2 cut(s)	923, 1955
Eco24I	GRGCYC	3 cut(s)	1217, 1515, 1967
Eco31I	GGTCTC	1 cut(s)	108
Eco47I	GGWCC	6 cut(s)	470, 866, 1022, 1728, 1858, 2337
Eco53kI	GAGCTC	2 cut(s)	1215, 1513
Eco57I	CTGAAG	3 cut(s)	783, 843, 1598
Eco88I	CYCGRG	1 cut(s)	2273
EcoICRI	GAGCTC	2 cut(s)	1215, 1513
EcoO109I	RGGNCCY	5 cut(s)	470, 1022, 1963, 1964, 2337
EcoRII	CCWGG	5 cut(s)	262, 358, 486, 593, 715
EcoT14I	CCWWGG	2 cut(s)	923, 1955
EcoT22I	ATGCAT	1 cut(s)	282
EcoT38I	GRGCYC	3 cut(s)	1217, 1515, 1967
EgeI	GGCGCC	1 cut(s)	2329
EheI	GGCGCC	1 cut(s)	2329
ErhI	CCWWGG	2 cut(s)	923, 1955
Esp3I	CGTCTC	1 cut(s)	221
FalI	AAGNNNNNCTT	2 cut(s)	2381, 2413
FaqI	GGGAC	3 cut(s)	152, 575, 1714
FbaI	TGATCA	1 cut(s)	108
FblI	GTMKAC	1 cut(s)	1400
FokI	GGATG	7 cut(s)	230, 289, 1103, 1307, 1989, 2119, 2243
FriOI	GRGCYC	3 cut(s)	1217, 1515, 1967
FspBI	CTAG	8 cut(s)	188, 561, 924, 1031, 1521, 1671, 1916, 1956
GlaI	GCGC	5 cut(s)	57, 78, 80, 2176, 2329
GsaI	CCCAGC	2 cut(s)	276, 1317
GsuI	CTGGAG	3 cut(s)	246, 606, 1080
HaeII	RGCGCY	1 cut(s)	2331
HaeIII	GGCC	9 cut(s)	120, 181, 249, 300, 485, 715, 757, 1954, 1965
HapII	CCGG	4 cut(s)	100, 754, 1868, 2166
HgaI	GACGC	1 cut(s)	154
HhaI	GCGC	5 cut(s)	58, 79, 81, 2177, 2330
Hin1I	GRCGYC	1 cut(s)	2328
Hin6I	GCGC	5 cut(s)	56, 77, 79, 2175, 2328
HinP1I	GCGC	5 cut(s)	56, 77, 79, 2175, 2328
HincII	GTYRAC	1 cut(s)	430
HindII	GTYRAC	1 cut(s)	430
HindIII	AAGCTT	2 cut(s)	1273, 2474
HpaII	CCGG	4 cut(s)	100, 754, 1868, 2166
HphI	GGTGA	7 cut(s)	743, 1039, 1337, 1433, 1883, 2116, 2155
Hpy166II	GTNNAC	9 cut(s)	414, 430, 539, 669, 814, 866, 1401, 1858, 2465
Hpy188I	TCNGA	8 cut(s)	108, 375, 579, 684, 1287, 1578, 1813, 2460
Hpy188III	TCNNGA	8 cut(s)	623, 1059, 1508, 1678, 1766, 2015, 2091, 2314
Hpy8I	GTNNAC	9 cut(s)	414, 430, 539, 669, 814, 866, 1401, 1858, 2465
Hpy99I	CGWCG	1 cut(s)	2420
HpyAV	CCTTC	9 cut(s)	200, 524, 661, 1035, 1561, 1573, 1742, 1772, 1954
HpyCH4III	ACNGT	9 cut(s)	42, 367, 659, 998, 1111, 1618, 2073, 2248, 2493
HpyCH4IV	ACGT	3 cut(s)	230, 2097, 2418
HpyF3I	CTNAG	6 cut(s)	171, 576, 1236, 1607, 1810, 2459
HpySE526I	ACGT	3 cut(s)	230, 2097, 2418
Hsp92I	GRCGYC	1 cut(s)	2328
HspAI	GCGC	5 cut(s)	56, 77, 79, 2175, 2328
KasI	GGCGCC	1 cut(s)	2327
KpnI	GGTACC	1 cut(s)	2062
Ksp22I	TGATCA	1 cut(s)	108
Kzo9I	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
LmnI	GCTCC	5 cut(s)	135, 625, 1061, 1518, 1753
LweI	GCATC	4 cut(s)	267, 467, 607, 1765
MaeI	CTAG	8 cut(s)	188, 561, 924, 1031, 1521, 1671, 1916, 1956
MaeII	ACGT	3 cut(s)	230, 2097, 2418
MaeIII	GTNAC	5 cut(s)	36, 644, 659, 1230, 2237
MalI	GATC	9 cut(s)	110, 1207, 1261, 1284, 1359, 1639, 1878, 2013, 2130
MboI	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
MfeI	CAATTG	1 cut(s)	903
MflI	RGATCY	3 cut(s)	1205, 1282, 2128
MhlI	GDGCHC	4 cut(s)	945, 1217, 1515, 1967
MlsI	TGGCCA	2 cut(s)	120, 249
MluNI	TGGCCA	2 cut(s)	120, 249
Mly113I	GGCGCC	1 cut(s)	2328
MlyI	GAGTC	1 cut(s)	635
MmeI	TCCRAC	1 cut(s)	1183
Mox20I	TGGCCA	2 cut(s)	120, 249
Mph1103I	ATGCAT	1 cut(s)	282
MscI	TGGCCA	2 cut(s)	120, 249
MslI	CAYNNNNRTG	3 cut(s)	1977, 2168, 2378
Msp20I	TGGCCA	2 cut(s)	120, 249
MspA1I	CMGCKG	3 cut(s)	124, 761, 1529
MspCI	CTTAAG	1 cut(s)	947
MspI	CCGG	4 cut(s)	100, 754, 1868, 2166
MspR9I	CCNGG	6 cut(s)	264, 360, 488, 595, 717, 1869
MunI	CAATTG	1 cut(s)	903
Mva1269I	GAATGC	1 cut(s)	1994
MvaI	CCWGG	5 cut(s)	264, 360, 488, 595, 717
MvnI	CGCG	2 cut(s)	56, 79
NarI	GGCGCC	1 cut(s)	2328
NciI	CCSGG	1 cut(s)	1869
NdeII	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
NmuCI	GTSAC	1 cut(s)	659
NsiI	ATGCAT	1 cut(s)	282
NspI	RCATGY	3 cut(s)	406, 1068, 1129
NspV	TTCGAA	2 cut(s)	292, 1120
OliI	CACNNNNGTG	1 cut(s)	2168
PauI	GCGCGC	1 cut(s)	77
PciI	ACATGT	1 cut(s)	1125
PctI	GAATGC	1 cut(s)	1994
PfeI	GAWTC	9 cut(s)	286, 745, 1472, 1504, 1681, 1689, 1897, 2213, 2317
PinAI	ACCGGT	1 cut(s)	2165
PleI	GAGTC	1 cut(s)	634
PluTI	GGCGCC	1 cut(s)	2331
PpsI	GAGTC	1 cut(s)	634
PpuMI	RGGWCCY	3 cut(s)	470, 1022, 2337
PscI	ACATGT	1 cut(s)	1125
Psp124BI	GAGCTC	2 cut(s)	1217, 1515
Psp5II	RGGWCCY	3 cut(s)	470, 1022, 2337
Psp6I	CCWGG	5 cut(s)	262, 358, 486, 593, 715
PspFI	CCCAGC	2 cut(s)	272, 1313
PspGI	CCWGG	5 cut(s)	262, 358, 486, 593, 715
PspOMI	GGGCCC	1 cut(s)	1963
PspPPI	RGGWCCY	3 cut(s)	470, 1022, 2337
PstI	CTGCAG	1 cut(s)	1629
PstNI	CAGNNNCTG	1 cut(s)	629
PsuI	RGATCY	3 cut(s)	1205, 1282, 2128
PteI	GCGCGC	1 cut(s)	77
PvuII	CAGCTG	2 cut(s)	761, 1529
RsaI	GTAC	5 cut(s)	323, 813, 2060, 2100, 2490
RsaNI	GTAC	5 cut(s)	322, 812, 2059, 2099, 2489
RseI	CAYNNNNRTG	3 cut(s)	1977, 2168, 2378
SacI	GAGCTC	2 cut(s)	1217, 1515
Sau3AI	GATC	9 cut(s)	108, 1205, 1259, 1282, 1357, 1637, 1876, 2011, 2128
SchI	GAGTC	1 cut(s)	635
ScrFI	CCNGG	6 cut(s)	264, 360, 488, 595, 717, 1869
SduI	GDGCHC	4 cut(s)	945, 1217, 1515, 1967
SfaNI	GCATC	4 cut(s)	267, 467, 607, 1765
SfcI	CTRYAG	4 cut(s)	780, 1218, 1625, 2322
SfoI	GGCGCC	1 cut(s)	2329
SfuI	TTCGAA	2 cut(s)	292, 1120
SgrAI	CRCCGGYG	1 cut(s)	2165
SinI	GGWCC	6 cut(s)	470, 866, 1022, 1728, 1858, 2337
SmiMI	CAYNNNNRTG	3 cut(s)	1977, 2168, 2378
SmlI	CTYRAG	2 cut(s)	947, 2149
SmoI	CTYRAG	2 cut(s)	947, 2149
SpeI	ACTAGT	1 cut(s)	1670
SsiI	CCGC	6 cut(s)	14, 74, 124, 804, 1331, 2155
SspDI	GGCGCC	1 cut(s)	2327
SspMI	CTAG	8 cut(s)	188, 561, 924, 1031, 1521, 1671, 1916, 1956
SstI	GAGCTC	2 cut(s)	1217, 1515
StyD4I	CCNGG	6 cut(s)	262, 358, 486, 593, 715, 1867
StyI	CCWWGG	2 cut(s)	923, 1955
TaaI	ACNGT	9 cut(s)	42, 367, 659, 998, 1111, 1618, 2073, 2248, 2493
TaiI	ACGT	3 cut(s)	233, 2100, 2421
TaqI	TCGA	7 cut(s)	114, 292, 853, 1120, 2010, 2112, 2385
TatI	WGTACW	1 cut(s)	2488
TauI	GCSGC	2 cut(s)	16, 77
TfiI	GAWTC	9 cut(s)	286, 745, 1472, 1504, 1681, 1689, 1897, 2213, 2317
TscAI	CASTG	4 cut(s)	370, 1023, 1260, 2498
TseFI	GTSAC	1 cut(s)	659
Tsp45I	GTSAC	1 cut(s)	659
TspGWI	ACGGA	1 cut(s)	540
TspRI	CASTG	4 cut(s)	370, 1023, 1260, 2498
Vha464I	CTTAAG	1 cut(s)	947
VpaK11BI	GGWCC	6 cut(s)	470, 866, 1022, 1728, 1858, 2337
XapI	RAATTY	7 cut(s)	696, 844, 1115, 1376, 2006, 2300, 2437
XceI	RCATGY	3 cut(s)	406, 1068, 1129
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2052
XmaJI	CCTAGG	2 cut(s)	923, 1955
XmiI	GTMKAC	1 cut(s)	1400
XspI	CTAG	8 cut(s)	188, 561, 924, 1031, 1521, 1671, 1916, 1956
Zsp2I	ATGCAT	1 cut(s)	282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.