pycom15g12390

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003198
Physical Location & Seq
Forward (+)
8471496 .. 8475038
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGGGCAAGGAAAGCTTATCAGAAGCTAGCATTGTAGCCTGTGAGGATAGCTCACCTGGACATAGTGATGATATTGATACTCAAGCATGCCATAGTCTTGACAACGAGGCCGGCTTGCTAACTTGTCGCGTGTGCCATTGTGCTGAATCGGACAAAAGGGGAGATGCAGCGTTAGGGTTTCTGGGTATTGTTCCTCCTTTACAAGAAGTATGTATAAGCAACGGGGAAGTAAAACCTCATAGCACGGTAGTCCCTAAATGTGATATATCTGCCCAGAAAAATGTTGGAAAAGAATCTGGTTTTTTGGAGTTTATAAGCCCTGATGGAGAGGTTTTCATATGTAGTGCTGATTTAGAGACTGGTTCATGTCACCATGAAGACATGTTAGTTGAGCTTGGTTGCTCTTGCAAAAATGACCTTGCTCTGGTACACTACGCTTGTGCACTCAAATGGTTTGTCAACCATGGATCCACTGTTTGTGAAATCTGCGGACGTGTTGCAAAAAATATCAGACCTTCAGACTTCAATAAGGTTATGGTCTCCTTAAAGGAGTATGAAACATTGAGGGAAAGAACTGCTAGGGGGGAAGCCGATGCCACACAGGTCAATACATGTACAGGTATAGATCCTGATGCTGTAGCTGCTATCAGAAGGCAACGACTGAGTGAAATTTCACTGTGGTTTACTCCATATGGTAATAGCAGTAGTAACAATAATACAGATAGCAATTCTGTTGCAATTTCACAAGTTGTACCGGAACAACCTTTGAATCCTGTCATTGTTCCTGCTGAAAATCCTGCAACCAAATGGGCTGTGGAAGGTACTGGAATTCTGCTTGCTACAGGGCTTCTTACTGTTACTCTTGCATGGCTTATAGCTCCTCGTGTTGGGAAGAAAACTGCCAGAAGCGGTCTTCATATTCTCCTTGGAGGAATTTGTGCTTTGACAGTTGTGGTTTTCTTTCGCTTTTTTGTGCTCACCAGGATCAAGTATGGACCTGCACGCTACTGGGCAATATTATTCGTTTTCTGGTTTCTAGTTTTTGGTATCTGGGCGTCACGGACACATGGTTCCCATACAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

362

Amino Acids

39.13

Weight (kDa)

5.78

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 127 - 163 7.8e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 1006
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 489, 909
AclWI GGATC 4 cut(s) 462, 475, 620, 992
AcsI RAATTY 3 cut(s) 669, 828, 933
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 1055
AfaI GTAC 4 cut(s) 429, 616, 753, 823
AfiI CCNNNNNNNGG 2 cut(s) 424, 887
AflIII ACRYGT 3 cut(s) 381, 493, 611
AgsI TTSAA 2 cut(s) 526, 769
AjiI CACGTC 1 cut(s) 494
AjnI CCWGG 2 cut(s) 55, 980
AloI GAACNNNNNNTCC 2 cut(s) 1054, 1086
AluBI AGCT 6 cut(s) 15, 26, 51, 394, 641, 878
AluI AGCT 6 cut(s) 15, 26, 51, 394, 641, 878
Alw21I GWGCWC 2 cut(s) 445, 978
Alw26I GTCTC 2 cut(s) 350, 544
Alw44I GTGCAC 1 cut(s) 441
AlwI GGATC 4 cut(s) 462, 475, 620, 992
AoxI GGCC 1 cut(s) 108
ApaLI GTGCAC 1 cut(s) 441
ApeKI GCWGC 2 cut(s) 167, 641
ApoI RAATTY 3 cut(s) 669, 828, 933
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AspS9I GGNCC 1 cut(s) 995
AsuHPI GGTGA 3 cut(s) 45, 362, 970
AsuNHI GCTAGC 1 cut(s) 26
AvaII GGWCC 1 cut(s) 995
BaeGI GKGCMC 1 cut(s) 445
BamHI GGATCC 1 cut(s) 467
BauI CACGAG 1 cut(s) 882
BbsI GAAGAC 2 cut(s) 384, 905
Bbv12I GWGCWC 2 cut(s) 445, 978
BbvI GCAGC 2 cut(s) 179, 628
BccI CCATC 1 cut(s) 317
BciT130I CCWGG 2 cut(s) 57, 982
BcoDI GTCTC 2 cut(s) 350, 544
BfaI CTAG 3 cut(s) 27, 579, 1037
BfmI CTRYAG 2 cut(s) 636, 840
BfuAI ACCTGC 1 cut(s) 1006
BisI GCNGC 2 cut(s) 168, 642
BlsI GCNGC 2 cut(s) 169, 643
Bme1390I CCNGG 2 cut(s) 57, 982
Bme18I GGWCC 1 cut(s) 995
BmgBI CACGTC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 995
BmiI GGNNCC 2 cut(s) 469, 1072
BmrFI CCNGG 2 cut(s) 57, 982
BmrI ACTGGG 1 cut(s) 1018
BmsI GCATC 3 cut(s) 154, 583, 622
BmtI GCTAGC 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 1018
BpiI GAAGAC 2 cut(s) 384, 905
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpuEI CTTGAG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 1055
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 463, 925
BsaWI WCCGGW 1 cut(s) 754
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 887
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 364, 829, 1013
BseBI CCWGG 2 cut(s) 57, 982
BseDI CCNNGG 2 cut(s) 463, 925
BseLI CCNNNNNNNGG 2 cut(s) 424, 887
BseMII CTCAG 1 cut(s) 653
BseNI ACTGG 3 cut(s) 364, 829, 1013
BseRI GAGGAG 1 cut(s) 870
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 2 cut(s) 179, 628
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 2 cut(s) 445, 978
BsiSI CCGG 2 cut(s) 111, 755
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 2 cut(s) 424, 887
BsmAI GTCTC 2 cut(s) 350, 544
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 110
Bso31I GGTCTC 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 445, 978
Bsp1407I TGTACA 1 cut(s) 614
Bsp143I GATC 3 cut(s) 467, 625, 984
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 2 cut(s) 489, 909
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 654
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 469, 1072
BspMI ACCTGC 1 cut(s) 1006
BspOI GCTAGC 1 cut(s) 30
BspPI GGATC 4 cut(s) 462, 475, 620, 992
BspTNI GGTCTC 1 cut(s) 544
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 614
BsrI ACTGG 3 cut(s) 364, 829, 1013
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 463, 925
BssMI GATC 3 cut(s) 467, 625, 984
BssNI GRCGYC 1 cut(s) 1055
BssSI CACGAG 1 cut(s) 882
BssT1I CCWWGG 2 cut(s) 463, 925
Bst2BI CACGAG 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 57, 982
Bst4CI ACNGT 5 cut(s) 247, 475, 678, 856, 949
BstACI GRCGYC 1 cut(s) 1055
BstAUI TGTACA 1 cut(s) 614
BstC8I GCNNGC 6 cut(s) 28, 88, 112, 116, 837, 1003
BstDEI CTNAG 1 cut(s) 662
BstDSI CCRYGG 1 cut(s) 463
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 3 cut(s) 470, 628, 987
BstMAI GTCTC 2 cut(s) 350, 544
BstMBI GATC 3 cut(s) 467, 625, 984
BstMWI GCNNNNNNNGC 2 cut(s) 12, 641
BstNI CCWGG 2 cut(s) 57, 982
BstNSI RCATGY 3 cut(s) 90, 385, 615
BstSCI CCNGG 2 cut(s) 55, 980
BstSFI CTRYAG 2 cut(s) 636, 840
BstSLI GKGCMC 1 cut(s) 445
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 179, 628
BstV2I GAAGAC 2 cut(s) 384, 905
BstX2I RGATCY 2 cut(s) 467, 625
BstYI RGATCY 2 cut(s) 467, 625
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 463
BtrI CACGTC 1 cut(s) 494
BtsIMutI CAGTG 2 cut(s) 471, 674
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 6 cut(s) 28, 88, 112, 116, 837, 1003
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 995
CseI GACGC 1 cut(s) 1044
Csp6I GTAC 4 cut(s) 428, 615, 752, 822
CviAII CATG 8 cut(s) 87, 366, 374, 382, 464, 612, 867, 1067
CviQI GTAC 4 cut(s) 428, 615, 752, 822
DdeI CTNAG 1 cut(s) 662
DpnI GATC 3 cut(s) 469, 627, 986
DpnII GATC 3 cut(s) 467, 625, 984
Eco130I CCWWGG 2 cut(s) 463, 925
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 995
Eco57I CTGAAG 1 cut(s) 501
EcoRI GAATTC 1 cut(s) 828
EcoRII CCWGG 2 cut(s) 55, 980
EcoT14I CCWWGG 2 cut(s) 463, 925
ErhI CCWWGG 2 cut(s) 463, 925
FaeI CATG 8 cut(s) 90, 369, 377, 385, 467, 615, 870, 1070
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 1 cut(s) 236
FatI CATG 8 cut(s) 86, 365, 373, 381, 463, 611, 866, 1066
FauNDI CATATG 2 cut(s) 338, 691
Fnu4HI GCNGC 2 cut(s) 168, 642
Fsp4HI GCNGC 2 cut(s) 168, 642
FspBI CTAG 3 cut(s) 27, 579, 1037
GluI GCNGC 2 cut(s) 168, 642
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 111, 755
HgaI GACGC 1 cut(s) 1044
Hin1I GRCGYC 1 cut(s) 1055
Hin1II CATG 8 cut(s) 90, 369, 377, 385, 467, 615, 870, 1070
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 3 cut(s) 146, 293, 769
HpaII CCGG 2 cut(s) 111, 755
HphI GGTGA 3 cut(s) 45, 362, 970
Hpy166II GTNNAC 4 cut(s) 430, 443, 460, 684
Hpy188I TCNGA 5 cut(s) 22, 151, 512, 520, 650
Hpy188III TCNNGA 2 cut(s) 98, 629
Hpy8I GTNNAC 4 cut(s) 430, 443, 460, 684
HpyAV CCTTC 3 cut(s) 525, 645, 812
HpyCH4III ACNGT 5 cut(s) 247, 475, 678, 856, 949
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 8 cut(s) 167, 408, 443, 500, 737, 800, 866, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 641
HpyF3I CTNAG 1 cut(s) 662
HpySE526I ACGT 1 cut(s) 493
Hsp92I GRCGYC 1 cut(s) 1055
Hsp92II CATG 8 cut(s) 90, 369, 377, 385, 467, 615, 870, 1070
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 3 cut(s) 467, 625, 984
LmnI GCTCC 1 cut(s) 883
Lsp1109I GCAGC 2 cut(s) 179, 628
LweI GCATC 3 cut(s) 154, 583, 622
MaeI CTAG 3 cut(s) 27, 579, 1037
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 4 cut(s) 368, 707, 856, 1056
MalI GATC 3 cut(s) 469, 627, 986
MboI GATC 3 cut(s) 467, 625, 984
MboII GAAGA 3 cut(s) 389, 904, 905
MflI RGATCY 2 cut(s) 467, 625
MhlI GDGCHC 2 cut(s) 445, 978
MluCI AATT 5 cut(s) 669, 727, 738, 828, 933
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 8 cut(s) 37, 100, 204, 246, 322, 558, 891, 923
MroNI GCCGGC 1 cut(s) 110
MseI TTAA 1 cut(s) 545
MslI CAYNNNNRTG 2 cut(s) 66, 448
MspI CCGG 2 cut(s) 111, 755
MspR9I CCNGG 2 cut(s) 57, 982
MvaI CCWGG 2 cut(s) 57, 982
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 12, 641
NaeI GCCGGC 1 cut(s) 112
NcoI CCATGG 1 cut(s) 463
NdeI CATATG 2 cut(s) 338, 691
NdeII GATC 3 cut(s) 467, 625, 984
NgoMIV GCCGGC 1 cut(s) 110
NheI GCTAGC 1 cut(s) 26
NlaIII CATG 8 cut(s) 90, 369, 377, 385, 467, 615, 870, 1070
NlaIV GGNNCC 2 cut(s) 469, 1072
NmuCI GTSAC 2 cut(s) 368, 1056
NspI RCATGY 3 cut(s) 90, 385, 615
PaeI GCATGC 1 cut(s) 90
PciI ACATGT 2 cut(s) 381, 611
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 146, 293, 769
PkrI GCNGC 2 cut(s) 169, 643
PscI ACATGT 2 cut(s) 381, 611
PsiI TTATAA 1 cut(s) 314
Psp6I CCWGG 2 cut(s) 55, 980
PspGI CCWGG 2 cut(s) 55, 980
PspN4I GGNNCC 2 cut(s) 469, 1072
PspPI GGNCC 1 cut(s) 995
PsuI RGATCY 2 cut(s) 467, 625
RsaI GTAC 4 cut(s) 429, 616, 753, 823
RsaNI GTAC 4 cut(s) 428, 615, 752, 822
RseI CAYNNNNRTG 2 cut(s) 66, 448
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 2 cut(s) 168, 642
Sau3AI GATC 3 cut(s) 467, 625, 984
Sau96I GGNCC 1 cut(s) 995
ScrFI CCNGG 2 cut(s) 57, 982
SduI GDGCHC 2 cut(s) 445, 978
SfaNI GCATC 3 cut(s) 154, 583, 622
SfcI CTRYAG 2 cut(s) 636, 840
SinI GGWCC 1 cut(s) 995
SmiMI CAYNNNNRTG 2 cut(s) 66, 448
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
SphI GCATGC 1 cut(s) 90
Sse9I AATT 5 cut(s) 669, 727, 738, 828, 933
SsiI CCGC 2 cut(s) 489, 909
SspI AATATT 1 cut(s) 1017
SspMI CTAG 3 cut(s) 27, 579, 1037
StyD4I CCNGG 2 cut(s) 55, 980
StyI CCWWGG 2 cut(s) 463, 925
TaaI ACNGT 5 cut(s) 247, 475, 678, 856, 949
TaiI ACGT 1 cut(s) 496
TasI AATT 5 cut(s) 669, 727, 738, 828, 933
TatI WGTACW 1 cut(s) 614
TfiI GAWTC 3 cut(s) 146, 293, 769
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 2 cut(s) 478, 681
TseFI GTSAC 2 cut(s) 368, 1056
TseI GCWGC 2 cut(s) 167, 641
Tsp45I GTSAC 2 cut(s) 368, 1056
TspDTI ATGAA 5 cut(s) 325, 354, 390, 570, 905
TspGWI ACGGA 1 cut(s) 1075
TspRI CASTG 2 cut(s) 478, 681
VneI GTGCAC 1 cut(s) 441
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 3 cut(s) 669, 828, 933
XceI RCATGY 3 cut(s) 90, 385, 615
XcmI CCANNNNNNNNNTGG 2 cut(s) 281, 811
XspI CTAG 3 cut(s) 27, 579, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.