pycom15g12730

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012878
Physical Location & Seq
Reverse (-)
8723301 .. 8726829
3529 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGAGAAGATGAACCAAGCGTTTGAGAAGGTGAAGATGCTGGTGGGTATGGAGGTGGACGAAGAAGACCAGGCTTCTGCTGCTCTACAGCAGGATTCCAACTTCATGGATGACTTCAATCGCAACTGCACATTGTCCACCAAACAGAGGCTGTACGGTTTCTCCATATGCTTGGCTGCCGGAATCACTTGTACGCTCTTGTCGATGCTTGTTTTCTTCCACCCAATCAAGTTTGCAATCACATTCACACTTGGAAATCTGCTTTCGCTTGGGAGCACAGCATTCCTCATCGGTCCTAAACGGCAAGTGTCAATGATGCTTGACCCTGTCCGAATTTATGCGACAGCGGTATACCTTGCAAGTATAGTAATTGCCTTGTTTTGTGCCCTTTATGTTCATAACAAGTTATTGACACTTTTGGCAATACTTTTAGAGTTTGGTGCTTTGATCTGGTACAGCTTGAGCTACATCCCTTTTGCAAGATCCATGGTGTCGAAGGTCATGGTATCGTGTTTTGACACTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016192 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

177

Amino Acids

19.72

Weight (kDa)

6.07

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 51 - 158 1.6e-32 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AciI CCGC 1 cut(s) 347
AclWI GGATC 1 cut(s) 477
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 3 cut(s) 155, 193, 455
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 1 cut(s) 118
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 2 cut(s) 459, 465
AluI AGCT 2 cut(s) 459, 465
Alw21I GWGCWC 1 cut(s) 278
AlwI GGATC 1 cut(s) 477
AlwNI CAGNNNCTG 1 cut(s) 151
ApeKI GCWGC 2 cut(s) 80, 176
ApoI RAATTY 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 67, 163
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 86
BisI GCNGC 2 cut(s) 81, 177
BlsI GCNGC 2 cut(s) 82, 178
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 3 cut(s) 27, 195, 306
BpiI GAAGAC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 147
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 2 cut(s) 115, 468
BseLI CCNNNNNNNGG 1 cut(s) 147
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 2 cut(s) 67, 163
BsgI GTGCAG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 180
BslI CCNNNNNNNGG 1 cut(s) 147
BsmI GAATGC 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 278, 388
Bsp143I GATC 2 cut(s) 447, 482
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 477
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 2 cut(s) 447, 482
BssNAI GTATAC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 486
Bst1107I GTATAC 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 158
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 115, 468
BstKTI GATC 2 cut(s) 450, 485
BstMBI GATC 2 cut(s) 447, 482
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 1 cut(s) 86
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 67, 163
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 482
BstXI CCANNNNNNTGG 2 cut(s) 106, 172
BstYI RGATCY 1 cut(s) 482
BstZ17I GTATAC 1 cut(s) 352
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 115, 468
BtsIMutI CAGTG 1 cut(s) 519
CaiI CAGNNNCTG 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 3 cut(s) 154, 192, 454
CviAII CATG 3 cut(s) 106, 487, 502
CviJI RGCY 5 cut(s) 74, 151, 176, 459, 465
CviKI_1 RGCY 5 cut(s) 74, 151, 176, 459, 465
CviQI GTAC 3 cut(s) 154, 192, 454
DpnI GATC 2 cut(s) 449, 484
DpnII GATC 2 cut(s) 447, 482
Eco130I CCWWGG 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 3 cut(s) 109, 490, 505
FatI CATG 3 cut(s) 105, 486, 501
FauNDI CATATG 1 cut(s) 167
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 2 cut(s) 81, 177
FokI GGATG 2 cut(s) 122, 455
Fsp4HI GCNGC 2 cut(s) 81, 177
GluI GCNGC 2 cut(s) 81, 177
HapII CCGG 1 cut(s) 180
Hin1II CATG 3 cut(s) 109, 490, 505
HinfI GANTC 2 cut(s) 95, 183
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 3 cut(s) 58, 138, 352
Hpy188I TCNGA 1 cut(s) 332
Hpy8I GTNNAC 3 cut(s) 58, 138, 352
HpyAV CCTTC 2 cut(s) 22, 490
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 4 cut(s) 129, 236, 359, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
Hsp92II CATG 3 cut(s) 109, 490, 505
Kzo9I GATC 2 cut(s) 447, 482
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 7 cut(s) 26, 56, 77, 83, 193, 339, 436
Lsp1109I GCAGC 2 cut(s) 67, 163
LweI GCATC 3 cut(s) 27, 195, 306
MalI GATC 2 cut(s) 449, 484
MboI GATC 2 cut(s) 447, 482
MboII GAAGA 5 cut(s) 19, 46, 74, 77, 208
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 278, 388
MluCI AATT 2 cut(s) 333, 369
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 3 cut(s) 46, 141, 296
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 80
NcoI CCATGG 1 cut(s) 486
NdeI CATATG 1 cut(s) 167
NdeII GATC 2 cut(s) 447, 482
NlaIII CATG 3 cut(s) 109, 490, 505
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 95, 183
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 82, 178
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 482
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 3 cut(s) 155, 193, 455
RsaNI GTAC 3 cut(s) 154, 192, 454
SatI GCNGC 2 cut(s) 81, 177
Sau3AI GATC 2 cut(s) 447, 482
Sau96I GGNCC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 2 cut(s) 278, 388
SetI ASST 6 cut(s) 33, 57, 357, 461, 467, 501
SfaNI GCATC 3 cut(s) 27, 195, 306
SfcI CTRYAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 2 cut(s) 333, 369
SsiI CCGC 1 cut(s) 347
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 203, 494
TaqII GACCGA 1 cut(s) 281
TasI AATT 2 cut(s) 333, 369
TfiI GAWTC 2 cut(s) 95, 183
TscAI CASTG 1 cut(s) 526
TseI GCWGC 2 cut(s) 80, 176
TspDTI ATGAA 3 cut(s) 26, 94, 386
TspRI CASTG 1 cut(s) 526
Tth111I GACNNNGTC 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.