pycom15g12750

Deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006626
Physical Location & Seq
Forward (+)
8737402 .. 8740000
2599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGATGAAATTATTTGACGCGCACTGTCACCTGCAAGACCCAAGAATCTTTGACAAGGCGCCCCAATTGATTGCAGCAACCCTCGATTCTGGCGTCGTTCGTTTTGCCGTCAATGGAGTCTCTGAGAAAGATTGGCACTTGGTCAAGCAGCTTGGGGAGTCCTATCCTTGCGTAATTCCATGCTTCGGTCTCCATCCCTGGTTTGTTGCAGAGAGGACACCAAATTGGCTTAACACTTTAAAACAATTCTTCGAGTCTACGCCCTCTGCAGCAGTTGGAGAGATAGGCTTGGACAAGGGTTCGCATGGGAAGAAGGTCGATTTCGCGGATCAGGTTAATGTATTCAGGCAACAACTTGAACTTGCAAAAGAGTTAAAAAGGCCAGCATCCATCCATTGTGTCCATGCTTTTGGTGACCTTCTTCAGATAATGAAGTACATAGATGCTGCCGTTTTCTCTATTATGTTAATCGTAGCTTGGCCTTTTCCTGCTGGCGTGATTCTTCATTCTTTCCTGGGTTCTGCTGAAATGGTTCCTGAATTTGCCAGTCTTGGTGCTTACTTTTCGTTCTCTGGATTTCTCATGTCCATGAAAGTAAACAAGGCAAAGAGGATGCTGAAGATGGTACCTTCTGAAAGAATATTACTGGAGACAGATGCACCTGATGCATTACCAAAGTCTGAGTTAGGTTCTCTGCATCTGGTTGAAGGAGATGCTTCCTTCCCTAAAGAGCTTCAGTCGCAAGAAATTTCCTCAGCTCCAGTGGCTAGTGCAAGCGATGCTGTAACACTTCCAAAAGTAGTACTAAATCACCCAGCAAATATTCACAATGTACTCATCTATGTCGCATCTTTGCTAGAGATGACCACAGAAGAACTTGCTGAAATAAGTTACCATAATGCAGTGCGCTTATTCTCTTACGATGTAGGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

312

Amino Acids

34.31

Weight (kDa)

5.83

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 5 - 306 2.1e-60 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 39
Acc65I GGTACC 1 cut(s) 625
AccB1I GGYRCC 2 cut(s) 58, 625
AccI GTMKAC 1 cut(s) 257
AccII CGCG 2 cut(s) 20, 326
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 2 cut(s) 539, 747
AcuI CTGAAG 3 cut(s) 407, 638, 719
AcyI GRCGYC 2 cut(s) 59, 93
AfaI GTAC 4 cut(s) 437, 627, 804, 834
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 2 cut(s) 359, 707
AjnI CCWGG 2 cut(s) 197, 513
AluBI AGCT 5 cut(s) 151, 476, 733, 758, 933
AluI AGCT 5 cut(s) 151, 476, 733, 758, 933
Alw26I GTCTC 3 cut(s) 124, 194, 644
AlwI GGATC 1 cut(s) 336
AoxI GGCC 2 cut(s) 380, 479
ApeKI GCWGC 4 cut(s) 74, 148, 269, 446
ApoI RAATTY 2 cut(s) 539, 747
Asp700I GAANNNNTTC 1 cut(s) 531
Asp718I GGTACC 1 cut(s) 625
AspLEI GCGC 3 cut(s) 22, 61, 909
AsuHPI GGTGA 3 cut(s) 20, 425, 803
BanI GGYRCC 2 cut(s) 58, 625
BbvCI CCTCAGC 1 cut(s) 754
BbvI GCAGC 4 cut(s) 86, 160, 281, 433
BccI CCATC 3 cut(s) 201, 398, 616
BceAI ACGGC 2 cut(s) 92, 434
BciT130I CCWGG 2 cut(s) 199, 515
BcoDI GTCTC 3 cut(s) 124, 194, 644
BfaI CTAG 2 cut(s) 768, 857
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 39
BisI GCNGC 4 cut(s) 75, 149, 270, 447
BlsI GCNGC 4 cut(s) 76, 150, 271, 448
BmcAI AGTACT 1 cut(s) 804
Bme1390I CCNGG 2 cut(s) 199, 515
BmiI GGNNCC 3 cut(s) 60, 534, 627
BmrFI CCNGG 2 cut(s) 199, 515
BmsI GCATC 9 cut(s) 395, 433, 603, 646, 655, 703, 706, 769, 857
BpmI CTGGAG 2 cut(s) 668, 744
Bpu10I CCTNAGC 1 cut(s) 754
BsaHI GRCGYC 2 cut(s) 59, 93
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 197, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 546, 651, 761
BseBI CCWGG 2 cut(s) 199, 515
BseDI CCNNGG 2 cut(s) 197, 514
BseGI GGATG 4 cut(s) 193, 386, 390, 618
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 3 cut(s) 114, 672, 768
BseNI ACTGG 3 cut(s) 546, 651, 761
BseXI GCAGC 4 cut(s) 86, 160, 281, 433
BseYI CCCAGC 1 cut(s) 814
Bsh1236I CGCG 2 cut(s) 20, 326
BshFI GGCC 2 cut(s) 382, 481
BshNI GGYRCC 2 cut(s) 58, 625
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 3 cut(s) 124, 194, 644
BsnI GGCC 2 cut(s) 382, 481
Bso31I GGTCTC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 1 cut(s) 326
BspANI GGCC 2 cut(s) 382, 481
BspCNI CTCAG 3 cut(s) 115, 673, 767
BspFNI CGCG 2 cut(s) 20, 326
BspLI GGNNCC 3 cut(s) 60, 534, 627
BspMAI CTGCAG 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 2 cut(s) 58, 625
BspTNI GGTCTC 1 cut(s) 194
BsrI ACTGG 3 cut(s) 546, 651, 761
BssECI CCNNGG 2 cut(s) 197, 514
BssMI GATC 1 cut(s) 328
BssNI GRCGYC 2 cut(s) 59, 93
Bst2UI CCWGG 2 cut(s) 199, 515
Bst4CI ACNGT 1 cut(s) 26
BstACI GRCGYC 2 cut(s) 59, 93
BstAPI GCANNNNNTGC 2 cut(s) 665, 779
BstC8I GCNNGC 3 cut(s) 384, 493, 775
BstDEI CTNAG 3 cut(s) 123, 681, 754
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 4 cut(s) 193, 386, 390, 618
BstFNI CGCG 2 cut(s) 20, 326
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 3 cut(s) 22, 61, 909
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 3 cut(s) 124, 194, 644
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 665, 739, 764, 779
BstNI CCWGG 2 cut(s) 199, 515
BstPI GGTNACC 1 cut(s) 413
BstSCI CCNGG 2 cut(s) 197, 513
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 2 cut(s) 20, 326
BstV1I GCAGC 4 cut(s) 86, 160, 281, 433
BstXI CCANNNNNNTGG 1 cut(s) 410
BsuRI GGCC 2 cut(s) 382, 481
BtgZI GCGATG 1 cut(s) 792
BtsCI GGATG 4 cut(s) 193, 386, 390, 618
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 3 cut(s) 22, 768, 909
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 384, 493, 775
CfoI GCGC 3 cut(s) 22, 61, 909
CseI GACGC 2 cut(s) 26, 82
Csp6I GTAC 4 cut(s) 436, 626, 803, 833
CviAII CATG 5 cut(s) 180, 305, 404, 583, 589
CviQI GTAC 4 cut(s) 436, 626, 803, 833
DdeI CTNAG 3 cut(s) 123, 681, 754
DinI GGCGCC 1 cut(s) 60
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DraI TTTAAA 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 194
Eco57I CTGAAG 3 cut(s) 407, 638, 719
Eco91I GGTNACC 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 197, 513
EcoT22I ATGCAT 1 cut(s) 670
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
FaeI CATG 5 cut(s) 183, 308, 407, 586, 592
FaiI YATR 9 cut(s) 181, 306, 405, 440, 464, 584, 590, 843, 897
FatI CATG 5 cut(s) 179, 304, 403, 582, 588
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 4 cut(s) 75, 149, 270, 447
FokI GGATG 4 cut(s) 180, 373, 377, 625
Fsp4HI GCNGC 4 cut(s) 75, 149, 270, 447
FspBI CTAG 2 cut(s) 768, 857
GlaI GCGC 3 cut(s) 21, 60, 908
GluI GCNGC 4 cut(s) 75, 149, 270, 447
GsaI CCCAGC 1 cut(s) 818
GsuI CTGGAG 2 cut(s) 668, 744
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 2 cut(s) 382, 481
HgaI GACGC 2 cut(s) 26, 82
HhaI GCGC 3 cut(s) 22, 61, 909
Hin1I GRCGYC 2 cut(s) 59, 93
Hin1II CATG 5 cut(s) 183, 308, 407, 586, 592
Hin6I GCGC 3 cut(s) 20, 59, 907
HinP1I GCGC 3 cut(s) 20, 59, 907
HinfI GANTC 6 cut(s) 45, 86, 117, 158, 254, 499
HphI GGTGA 3 cut(s) 20, 425, 803
Hpy166II GTNNAC 2 cut(s) 258, 598
Hpy188I TCNGA 4 cut(s) 124, 426, 634, 682
Hpy188III TCNNGA 2 cut(s) 536, 573
Hpy8I GTNNAC 2 cut(s) 258, 598
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 5 cut(s) 307, 428, 639, 701, 730
HpyCH4III ACNGT 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 4 cut(s) 665, 739, 764, 779
HpyF3I CTNAG 3 cut(s) 123, 681, 754
Hsp92I GRCGYC 2 cut(s) 59, 93
Hsp92II CATG 5 cut(s) 183, 308, 407, 586, 592
HspAI GCGC 3 cut(s) 20, 59, 907
KasI GGCGCC 1 cut(s) 58
KpnI GGTACC 1 cut(s) 629
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 763
Lsp1109I GCAGC 4 cut(s) 86, 160, 281, 433
LweI GCATC 9 cut(s) 395, 433, 603, 646, 655, 703, 706, 769, 857
MaeI CTAG 2 cut(s) 768, 857
MaeIII GTNAC 4 cut(s) 26, 413, 784, 890
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 6 cut(s) 241, 322, 413, 494, 631, 884
MfeI CAATTG 1 cut(s) 65
MluCI AATT 7 cut(s) 8, 65, 174, 223, 245, 539, 747
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 3 cut(s) 126, 167, 263
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 5 cut(s) 92, 207, 274, 603, 763
Mph1103I ATGCAT 1 cut(s) 670
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 5 cut(s) 231, 239, 336, 374, 467
MslI CAYNNNNRTG 1 cut(s) 587
MspR9I CCNGG 2 cut(s) 199, 515
MunI CAATTG 1 cut(s) 65
MvaI CCWGG 2 cut(s) 199, 515
MvnI CGCG 2 cut(s) 20, 326
MwoI GCNNNNNNNGC 4 cut(s) 665, 739, 764, 779
NarI GGCGCC 1 cut(s) 59
NdeII GATC 1 cut(s) 328
NlaIII CATG 5 cut(s) 183, 308, 407, 586, 592
NlaIV GGNNCC 3 cut(s) 60, 534, 627
NmuCI GTSAC 2 cut(s) 26, 413
NsiI ATGCAT 1 cut(s) 670
PaqCI CACCTGC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 45, 86, 499
PkrI GCNGC 4 cut(s) 76, 150, 271, 448
PleI GAGTC 3 cut(s) 125, 166, 262
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 3 cut(s) 125, 166, 262
Psp6I CCWGG 2 cut(s) 197, 513
PspEI GGTNACC 1 cut(s) 413
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 2 cut(s) 197, 513
PspN4I GGNNCC 3 cut(s) 60, 534, 627
PstI CTGCAG 1 cut(s) 271
RsaI GTAC 4 cut(s) 437, 627, 804, 834
RsaNI GTAC 4 cut(s) 436, 626, 803, 833
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 5 cut(s) 231, 239, 336, 374, 467
SatI GCNGC 4 cut(s) 75, 149, 270, 447
Sau3AI GATC 1 cut(s) 328
ScaI AGTACT 1 cut(s) 804
SchI GAGTC 3 cut(s) 126, 167, 263
ScrFI CCNGG 2 cut(s) 199, 515
SfaNI GCATC 9 cut(s) 395, 433, 603, 646, 655, 703, 706, 769, 857
SfcI CTRYAG 1 cut(s) 267
SfoI GGCGCC 1 cut(s) 60
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 7 cut(s) 8, 65, 174, 223, 245, 539, 747
SsiI CCGC 1 cut(s) 326
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 2 cut(s) 642, 823
SspMI CTAG 2 cut(s) 768, 857
StyD4I CCNGG 2 cut(s) 197, 513
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 3 cut(s) 84, 252, 318
TaqII GACCGA 1 cut(s) 176
TasI AATT 7 cut(s) 8, 65, 174, 223, 245, 539, 747
TatI WGTACW 3 cut(s) 435, 802, 832
TfiI GAWTC 3 cut(s) 45, 86, 499
Tru1I TTAA 5 cut(s) 231, 239, 336, 374, 467
Tru9I TTAA 5 cut(s) 231, 239, 336, 374, 467
TscAI CASTG 3 cut(s) 29, 768, 909
TseFI GTSAC 2 cut(s) 26, 413
TseI GCWGC 4 cut(s) 74, 148, 269, 446
Tsp45I GTSAC 2 cut(s) 26, 413
TspDTI ATGAA 4 cut(s) 20, 446, 494, 605
TspRI CASTG 3 cut(s) 29, 768, 909
XapI RAATTY 2 cut(s) 539, 747
XmiI GTMKAC 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 2 cut(s) 768, 857
ZrmI AGTACT 1 cut(s) 804
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.