pycom15g14140

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004795
Physical Location & Seq
Reverse (-)
9625036 .. 9635325
10290 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4875 bp
ATGGACGGCGCTGGCGGAAGTTTTCCACCGTCTGATCAGGGCGGTTCGTCTACCGACCGGGTCAAGTCCGGTCATGGAGCTTCGAAGCCGGAGAAAGACCCGAGGAAGGACGCCAGAAAGTATCAATTGGACCTGTGCAAGAAAGCTTTGGAGGAGAACGTAATTGTGTATCTAGGGACTGGTTGTGGGAAAACCCGCATAGCGGTCATGCTTATATACGAGATGCGCCACTTGATCCGCAAACCCCAAAAGAGCGTATGCGTGTTTCTTGCACCCACAGTGGCTCTGGTTCAACAGCAAGCTAGGGTTATAGAAGATTCCCTTGGTTTCGATGTTGGGATTTATTGTGGAGGCTCTAACCAATTGAAGTATCACCAAGACTGGGAAAAAGAGATTGAACAATATGAGGTTTTTGTTATGATCCCTGAAGTACTGCTACGAAACTTATGTCACTGTTTCATTAAGATGGAGTCAATTTCTTTGTTGATATTTGATGAATGTCATCATGCTCAAGTCCAAAGCAACCATGCCTATGCAGAAATTATGAAATTGTTTTACAATGCTAATACCACAAAACATCCTCGTATATTTGGCATGACGGCATCTCCAGTTGTAGGGAAAGGTGCTTCGAGTCGGGAAAACTTATCAAAATGCATCAATAGTCTTGAAAGTATACTTGATGCTAAGGTTTATTCAGTTGATAAGAAAGAATTGGAATGTTTTGTCTCATCTCCTGTCATCACAGTGTATGATTATGGTCCAGTTATAAGCGACGCATCCAGCCCCTATATCACTTACTACACTAAACTTGAGCAGATGAAACACCAGTGTATAGCAGAACTTGGTAAGAAGACAAATGATCACCAAAGTCTAAGGAGTACAAAGAAACTGTTGAACAGAATGCATGAAAGTATACGGTTTTGCTTGGAAAATCTTGGCCTTTGGGGAGCATTAAAAGCTAGTCATGTTCTTTTGAGTGGCGATCACTTGGAAAGGAGTGAACTAATAGAGGAGGAAGGAAATAATGGTGATGATTCTCTATGTGTTAGATATCTGACTCAGGTTGATGGCATCCTTACTACTGACTGCTTAAAAGATTCCATTGCTGATGATCTATCTTCTATAGATATTTTAAAGGAGCCATTTTTCTCAAGAAAGCTCTTACGTCTTATTGGAGTTCTTTCATCCTTTAGGTTGCAACAAAATATGAAATGCATAATTTTTGTCAATAGAATTGTTACTGCGAGTTCCTTGTCTTACATACTACAACGCCTCAAGTTTCTAGCATCTTGGAAGTGTGATTTTCTTGTAGGAGTCAACTCTAGACTGATGAATATGTCAAGGAAGAAGATGAAAATCATTCTTGATAAGTTCCGATCTGGAGAGTTGAATCTATTGGTTGCAACTAAAGTTGGTGAAGAAGGACTAGATATTCAGACGTGCTGCCTTGTGATACGATTTGATCTTCCAGAAACTGTTGCTAGCTTTATACAATCACGAGGTCGTGCACGGATGCCTCAGTCTGAATATGCATTTTTGGTAAACAGTGGCAACCAGAAAGAGAGAGATTTAATAGAAACCTTCAGACAAGATGAAGCTCACATGAACATGGAAATTGCTTTCAGAACATCCAGTGAGACATTTGTTGGTTCGGCAGATAGGGTGTATAAAGTTGATTCTTCTGGTGCTTCCATCAGTTCTGGATATAGCATCTCATTGTTACACAAGTATTGTTCAAAGCTTCCACATGATGAGTACTTTGACCCCAATCCAAAATTCTTTTTTTTTGATGACTTGGGGGGAACTGTTTGTCAGATAATTTTACCTTCCAATGCGCCAATCCATCAAATTGTCGGAACACAACAATCTTCGATGGAAGATGCCAAAAAAGATGTTTGTCTAAAGGCCATTGAAGAATTGCATAAATTAGGTGCCTTAAATGACTTTCTCTTACCACAGCAAGACAATGCAAATGAGGTGGAGTTGGTGTCTTCTGATTCTGACAGTACCGAAGATGAGGATCCAAGAGGAGAACTACATGAAATGCTAGTTCCTGATGCACTAAAAGAGCCATGGAGTAGTTCGGAGGACCTTGTTTCCCTCAGCTCATACTATATAAAATTTAACCCTGTTCCTGAGGACAGGATCTATAGAAGCTTTGGTCTATTTGTCAAGGCTCCTCTCCCAGTTGAAGCCGAAACAATGGAACTTGATCTTCATCTCGCTCATAGTAGATCTGTGATTACAAAGTTGGTCCCTTCAGGATTTGCCAATTTTGGCAAAGATGAGATCGTGCTGGCACAAAATTTTCAAGAAATGTTCCTCAAGCTCGTCCTTGACCGAATGAAATTTGTTTCTGATTATGTTCCATTGGGAAGGCATGATAGATCAAGCTCATCAACCTTCTACCTGTTGCTTCCAGTAATGTTGGGTAACGATGATAAGATAGCAAGCATTGATTGGGGGACTATCAGAAAATGTTTATCATCCCCAGTGTTCAGGGCTCCAGGAGATGCTACAGAGAAAGTTCCTTCTTTGGATATTCGGCTTGCATCAGGTTACAAAAGCATAAGGGATATTCAGAATAGTTTAGTCTATGTCCCATATAAGAAATCATTTTACTTCATCACTGATGTTGTTCGAGAAAAGAATGGCTACAGCCAATATAAAAACTCGGGCACTTTAACCTATGTGGACCATTTAATTCAAAAATTTGGCATTCACCTTAAATACCCTGAGCAACAGCTTCTGCATGCCAAACCACTTTTCTCTTTACACAACTTGCTACACAACCGAAGGCAAGAAGATTCGGGACCGACACAACTAGAGGAGTATTTTATTAGCTTGCCACCTGAGCTTTGCGAGTTGAAGGTAATAGGATTTTCCAAAGATATTGGGAGTTCAATATCTTTGTTACCATCACTCATGCACCGGCTGGAAAACCTGCTTGTCGCCATCGAACTGAAGCATGTATTAGCTGCTTCATTCCCAGAGGGAGGTGCAGTTACTGCCAAGAGAGTTCTAGAGGCACTCACAACTGAGAAGTGCCAGGAGCGGCTTTCCCTTGAAAGGCTTGAAATACTTGGTGATGCTTTCCTTAAGTTTGCTGTTGGGCGACACTTCTTCCTTCGGCATGGTAGCCTTGATGAAGGGGGACTAACAAGGAAGCGTTCTAATGTGGTAAACAATTCCAATTTATTCAAGCTAGCCATGAGAAGCAACTTACAGGTATACATACGGGATCAACCATTTGAACCCTCTCAGTTCTTTGCTTTAGGCCGACCTTGCCCAAGAATTTGTAACGAGGAGACAGTAGGAGCTATTCATTCTCATGACTTATCCGGTGATGTAAATCACACAAGTGCTAGTGAGGTCAGATGCAGTAAGGGTCACCATTGGTTATATAAGAAAACCATTGCTGATGTTGTTGAAGCCCTTGTTGGAGCATTCGTAGTTGACAGTGGCTTTATAGCTGCAACTGCATTTCTTAAGTGGATTGGCATACAAGTAGACTTCGAACCATCACAAGTTACTGAAGCATGCATAGCAAGCTCTAGATACATCCCACTTTCTGCTTGCATGAACATTGCTGCCCTTGAAAATTTATTAGGGTATAAGTTTCTCCACAGAGGTCTGCTCTTACAGGCATTTGTACATCCTTCTTACAACAAGCATGGAGGAGGCTGCTATCAGAGATTGGAGTTTCTTGGGGATGCCGTCTTGGATTACTTGATCACCTCGTACCTGTATTCAGTTTATCCAAAGTTGAAGCCTGGTCAATTAACGGATTTGAGATCAGTGTCTGTGAATAATAAAGCCTTTGCTAATGTTGCAGTAGATCGGTCCTTCCACAAGTTTCTAATCAGTGATTCCTCTAGCCTTTCAGAGGCGATAGAAAGTTATGTGGGCTTCATTGAAACAGCTGCATCAGATAGAGGTCTGCTTGATGGGCCCAAATGTCCAAAGGCTCTGGGCGACTTGGTAGAGTCCTGTCTGGGCGCAATTCTTCTTGATACAGGGTTCAACTTGAATCGTGTTTGGGAGATAATGCTATCCTTTCTGAAACCAATTATGAGCTTTTCCAGCTTGCAACTTAGTCCTATTAGGGAACTACGTGAACTTTGCCAAGCTCATTCATGGGGTCTTGAGTTTGTACCAACAAAAATGGGTAAAAAGTATTCAGTTTGTGCAACAGTGAATGGGAATAATGTTTGTGTATCTGCTTCTACGACCAGCTTGAACAAAAAGGATGGAATAAGAATTTGTGCACAACTGATTTTCGACAAGTTGAAGGCTCAAGGCAACATACCTATGACTAACTCATTGGAGGAAGTCTTAAAGTCAAGCAGCGAGATGGAAGCCAAACTTATTGGATATGATGAAACACCAATAGATGTTGTGCTTCTTGATGCAAGTGGATTTGATAACTTAAATGTACAAGAAGTTTCCCGCCGAAATTTTAACTCCCAAGGGCATCCTATCAATCAAGAGAGAAACGGTCACTCTTCCTGCAGAAAACCTGTACGACAATCCCCATCTTCATCTGGAGCAGTCAAAATGCGGCCCAATAAGACCAGGGAAAATTCTAAGAATGATGCTAACTCGCAATCCGGAGGTACACACACGGCAACATCAAGATCTCGTTTGTATGAAGTTTGTGCCGCTAACTACTGGGAAACTCCCTTATTTGAGTGCTGCAATGAAGAAGGACCAAGCCACTTAAAATCGTTCACCTACAAGGCTGTAGTAAAGATAGAAGAAGATCCAGATACGTTGTTAGAGTGCTTCGGAGAACCTAATATGAAAAAGAAAGCAGCAGCAGAGAGTGCGGCAGAAGGGGCACTTTGGTACTTGAAAAACAGAGGTTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006351 GO:0006353 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009798 GO:0009888 GO:0009892 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010050 GO:0010073 GO:0010074 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010599 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048507 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065001 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097659 GO:0098542 GO:0098586 GO:0098727 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1625

Amino Acids

181.76

Weight (kDa)

6.47

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 38 - 203 2.2e-15 Type III restriction enzyme, res subunit
DEAD PF00270 40 - 203 3.2e-19 DEAD/DEAH box helicase
Helicase_C PF00271 394 - 503 1.2e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 570 - 655 3.7e-24 Dicer dimerisation domain
PAZ PF02170 858 - 978 8.7e-09 PAZ domain
Ribonucleas_3_3 PF14622 1022 - 1080 9.3e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1023 - 1155 6.4e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1211 - 1342 6.2e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1231 - 1340 2.4e-21 Ribonuclease III domain
DND1_DSRM PF14709 1545 - 1619 3.5e-14 double strand RNA binding domain from DEAD END PROTEIN 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 2952
AccB1I GGYRCC 1 cut(s) 1931
AccBSI CCGCTC 1 cut(s) 3055
AccI GTMKAC 5 cut(s) 50, 673, 913, 3231, 3510
AccIII TCCGGA 1 cut(s) 4610
AclWI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4757
AcuI CTGAAG 5 cut(s) 447, 1567, 2244, 2984, 3555
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 7 cut(s) 106, 202, 382, 614, 2996, 3069, 3886
AflII CTTAAG 2 cut(s) 3098, 3488
AjiI CACGTC 1 cut(s) 1440
AjnI CCWGG 4 cut(s) 2506, 3048, 3772, 4574
AjuI GAANNNNNNNTTGG 8 cut(s) 306, 338, 1760, 1792, 2750, 2782, 3423, 3455
AleI CACNNNNGTG 1 cut(s) 3360
Alw21I GWGCWC 2 cut(s) 1510, 4270
Alw26I GTCTC 3 cut(s) 730, 1631, 3302
Alw44I GTGCAC 2 cut(s) 1506, 4266
AlwI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4757
AlwNI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
Ama87I CYCGRG 2 cut(s) 100, 2674
Aor13HI TCCGGA 1 cut(s) 4610
AoxI GGCC 5 cut(s) 937, 1905, 3277, 3950, 4562
ApaI GGGCCC 1 cut(s) 3954
ApaLI GTGCAC 2 cut(s) 1506, 4266
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AspLEI GCGC 4 cut(s) 11, 228, 1837, 4001
AsuC2I CCSGG 1 cut(s) 59
AsuII TTCGAA 2 cut(s) 83, 3516
AsuNHI GCTAGC 2 cut(s) 1481, 3205
AvaI CYCGRG 2 cut(s) 100, 2674
AvaII GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4709
AxyI CCTNAGG 1 cut(s) 2136
BaeGI GKGCMC 5 cut(s) 1510, 2681, 3954, 4270, 4843
BamHI GGATCC 1 cut(s) 2020
BanI GGYRCC 1 cut(s) 1931
BanII GRGCYC 2 cut(s) 2506, 3954
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
BauI CACGAG 1 cut(s) 1497
BbsI GAAGAC 2 cut(s) 857, 1983
Bbv12I GWGCWC 2 cut(s) 1510, 4270
BbvCI CCTCAGC 1 cut(s) 2102
BceAI ACGGC 4 cut(s) 22, 615, 3701, 4641
BciT130I CCWGG 4 cut(s) 2508, 3050, 3774, 4576
BclI TGATCA 3 cut(s) 34, 859, 3732
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 3 cut(s) 730, 1631, 3302
BfmI CTRYAG 6 cut(s) 1122, 2149, 2517, 2656, 4510, 4743
BfoI RGCGCY 1 cut(s) 12
BfrI CTTAAG 2 cut(s) 3098, 3488
BfuAI ACCTGC 1 cut(s) 2952
BglII AGATCT 2 cut(s) 2234, 4637
BmcAI AGTACT 2 cut(s) 432, 1757
Bme1390I CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4576
Bme18I GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4709
BmeT110I CYCGRG 2 cut(s) 100, 2674
BmgBI CACGTC 1 cut(s) 1440
BmiI GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3952
BmrFI CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4576
BmrI ACTGGG 4 cut(s) 391, 2180, 2486, 4681
BmtI GCTAGC 2 cut(s) 1485, 3209
BmuI ACTGGG 4 cut(s) 391, 2180, 2486, 4681
BpiI GAAGAC 2 cut(s) 857, 1983
BplI GAGNNNNNCTC 2 cut(s) 3621, 3653
BpmI CTGGAG 4 cut(s) 591, 1401, 2490, 4566
Bpu10I CCTNAGC 4 cut(s) 684, 2102, 2736, 2853
Bpu14I TTCGAA 2 cut(s) 83, 3516
BpuEI CTTGAG 7 cut(s) 495, 830, 1135, 1259, 2309, 4166, 4281
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 4115
BsaBI GATNNNNATC 4 cut(s) 1355, 2019, 2217, 3351
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 5 cut(s) 101, 322, 2072, 4468, 4575
BsaWI WCCGGW 3 cut(s) 68, 3341, 4610
BsaXI ACNNNNNCTCC 8 cut(s) 589, 619, 1973, 2003, 2988, 3018, 3435, 3465
Bsc4I CCNNNNNNNGG 7 cut(s) 106, 202, 382, 614, 2996, 3069, 3886
Bse118I RCCGGY 1 cut(s) 2931
Bse21I CCTNAGG 1 cut(s) 2136
Bse3DI GCAATG 4 cut(s) 1101, 3414, 3585, 4705
Bse8I GATNNNNATC 4 cut(s) 1355, 2019, 2217, 3351
BseAI TCCGGA 1 cut(s) 4610
BseBI CCWGG 4 cut(s) 2508, 3050, 3774, 4576
BseDI CCNNGG 5 cut(s) 101, 322, 2072, 4468, 4575
BseJI GATNNNNATC 4 cut(s) 1355, 2019, 2217, 3351
BseLI CCNNNNNNNGG 7 cut(s) 106, 202, 382, 614, 2996, 3069, 3886
BseMI GCAATG 4 cut(s) 1101, 3414, 3585, 4705
BseMII CTCAG 8 cut(s) 1073, 1532, 2116, 2127, 2727, 2844, 3030, 3275
BseRI GAGGAG 7 cut(s) 167, 1025, 2043, 2169, 2843, 3320, 3693
BseSI GKGCMC 5 cut(s) 1510, 2681, 3954, 4270, 4843
BsgI GTGCAG 1 cut(s) 3021
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 5 cut(s) 939, 1907, 3279, 3952, 4564
BshNI GGYRCC 1 cut(s) 1931
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 1510, 4270
BsiHKCI CYCGRG 2 cut(s) 100, 2674
BsiSI CCGG 6 cut(s) 58, 69, 89, 2932, 3342, 4611
BslFI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
BslI CCNNNNNNNGG 7 cut(s) 106, 202, 382, 614, 2996, 3069, 3886
BsmAI GTCTC 3 cut(s) 730, 1631, 3302
BsmFI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
BsmI GAATGC 3 cut(s) 906, 2718, 3446
BsnI GGCC 5 cut(s) 939, 1907, 3279, 3952, 4564
BsoBI CYCGRG 2 cut(s) 100, 2674
Bsp119I TTCGAA 2 cut(s) 83, 3516
Bsp120I GGGCCC 1 cut(s) 3950
Bsp1286I GDGCHC 6 cut(s) 1510, 2506, 2681, 3954, 4270, 4843
Bsp13I TCCGGA 1 cut(s) 4610
Bsp1407I TGTACA 2 cut(s) 3652, 4435
Bsp19I CCATGG 1 cut(s) 2072
BspANI GGCC 5 cut(s) 939, 1907, 3279, 3952, 4564
BspCNI CTCAG 8 cut(s) 1072, 1531, 2115, 2128, 2728, 2845, 3031, 3274
BspEI TCCGGA 1 cut(s) 4610
BspHI TCATGA 1 cut(s) 3331
BspLI GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3952
BspMAI CTGCAG 1 cut(s) 4514
BspMI ACCTGC 1 cut(s) 2952
BspOI GCTAGC 2 cut(s) 1485, 3209
BspPI GGATC 7 cut(s) 229, 415, 2015, 2028, 2153, 3249, 4757
BspT104I TTCGAA 2 cut(s) 83, 3516
BspT107I GGYRCC 1 cut(s) 1931
BspTI CTTAAG 2 cut(s) 3098, 3488
BsrBI CCGCTC 1 cut(s) 3055
BsrDI GCAATG 4 cut(s) 1101, 3414, 3585, 4705
BsrFI RCCGGY 1 cut(s) 2931
BsrGI TGTACA 2 cut(s) 3652, 4435
BssAI RCCGGY 1 cut(s) 2931
BssECI CCNNGG 5 cut(s) 101, 322, 2072, 4468, 4575
BssNAI GTATAC 3 cut(s) 674, 914, 3232
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 1497
BssT1I CCWWGG 3 cut(s) 322, 2072, 4468
Bst1107I GTATAC 3 cut(s) 674, 914, 3232
Bst2BI CACGAG 1 cut(s) 1497
Bst2UI CCWGG 4 cut(s) 2508, 3050, 3774, 4576
Bst6I CTCTTC 1 cut(s) 4510
BstACI GRCGYC 1 cut(s) 111
BstAFI CTTAAG 2 cut(s) 3098, 3488
BstAPI GCANNNNNTGC 2 cut(s) 3008, 4826
BstAUI TGTACA 2 cut(s) 3652, 4435
BstBAI YACGTR 1 cut(s) 4115
BstBI TTCGAA 2 cut(s) 83, 3516
BstDSI CCRYGG 1 cut(s) 2072
BstEII GGTNACC 1 cut(s) 3389
BstENI CCTNNNNNAGG 1 cut(s) 3884
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 4 cut(s) 11, 228, 1837, 4001
BstMAI GTCTC 3 cut(s) 730, 1631, 3302
BstMCI CGRYCG 1 cut(s) 58
BstNI CCWGG 4 cut(s) 2508, 3050, 3774, 4576
BstNSI RCATGY 3 cut(s) 2756, 2972, 3543
BstPI GGTNACC 1 cut(s) 3389
BstSCI CCNGG 5 cut(s) 57, 2506, 3048, 3772, 4574
BstSFI CTRYAG 6 cut(s) 1122, 2149, 2517, 2656, 4510, 4743
BstSLI GKGCMC 5 cut(s) 1510, 2681, 3954, 4270, 4843
BstV2I GAAGAC 2 cut(s) 857, 1983
BstX2I RGATCY 5 cut(s) 2020, 2145, 2234, 4637, 4762
BstYI RGATCY 5 cut(s) 2020, 2145, 2234, 4637, 4762
BstZ17I GTATAC 3 cut(s) 674, 914, 3232
Bsu36I CCTNAGG 1 cut(s) 2136
BsuRI GGCC 5 cut(s) 939, 1907, 3279, 3952, 4564
BtgI CCRYGG 1 cut(s) 2072
BtrI CACGTC 1 cut(s) 1440
BveI ACCTGC 1 cut(s) 2952
CaiI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
CciI TCATGA 1 cut(s) 3331
CfoI GCGC 4 cut(s) 11, 228, 1837, 4001
Cfr10I RCCGGY 1 cut(s) 2931
CseI GACGC 2 cut(s) 119, 782
CspCI CAANNNNNGTGG 2 cut(s) 1959, 1994
DraI TTTAAA 1 cut(s) 1134
Eam1104I CTCTTC 1 cut(s) 4510
EarI CTCTTC 1 cut(s) 4510
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 322, 2072, 4468
Eco24I GRGCYC 2 cut(s) 2506, 3954
Eco32I GATATC 1 cut(s) 1052
Eco47I GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4709
Eco57I CTGAAG 5 cut(s) 447, 1567, 2244, 2984, 3555
Eco81I CCTNAGG 1 cut(s) 2136
Eco88I CYCGRG 2 cut(s) 100, 2674
Eco91I GGTNACC 1 cut(s) 3389
EcoNI CCTNNNNNAGG 1 cut(s) 3884
EcoO109I RGGNCCY 1 cut(s) 2089
EcoO65I GGTNACC 1 cut(s) 3389
EcoRII CCWGG 4 cut(s) 2506, 3048, 3772, 4574
EcoRV GATATC 1 cut(s) 1052
EcoT14I CCWWGG 3 cut(s) 322, 2072, 4468
EcoT22I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
EcoT38I GRGCYC 2 cut(s) 2506, 3954
ErhI CCWWGG 3 cut(s) 322, 2072, 4468
FalI AAGNNNNNCTT 6 cut(s) 306, 338, 4350, 4382, 4827, 4859
FaqI GGGAC 6 cut(s) 190, 2240, 2479, 2585, 2826, 3168
FauI CCCGC 2 cut(s) 203, 4457
FbaI TGATCA 3 cut(s) 34, 859, 3732
FblI GTMKAC 5 cut(s) 50, 673, 913, 3231, 3510
FriOI GRGCYC 2 cut(s) 2506, 3954
GlaI GCGC 4 cut(s) 10, 227, 1836, 4000
GsuI CTGGAG 4 cut(s) 591, 1401, 2490, 4566
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 5 cut(s) 939, 1907, 3279, 3952, 4564
HapII CCGG 6 cut(s) 58, 69, 89, 2932, 3342, 4611
HgaI GACGC 2 cut(s) 119, 782
HhaI GCGC 4 cut(s) 11, 228, 1837, 4001
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 4 cut(s) 9, 226, 1835, 3999
HinP1I GCGC 4 cut(s) 9, 226, 1835, 3999
HincII GTYRAC 2 cut(s) 1318, 3457
HindII GTYRAC 2 cut(s) 1318, 3457
HindIII AAGCTT 3 cut(s) 144, 1739, 2155
HpaII CCGG 6 cut(s) 58, 69, 89, 2932, 3342, 4611
Hpy99I CGWCG 1 cut(s) 776
HpyCH4IV ACGT 5 cut(s) 159, 1165, 1439, 4114, 4772
HpySE526I ACGT 5 cut(s) 159, 1165, 1439, 4114, 4772
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 4 cut(s) 9, 226, 1835, 3999
Kpn2I TCCGGA 1 cut(s) 4610
Ksp22I TGATCA 3 cut(s) 34, 859, 3732
LmnI GCTCC 9 cut(s) 77, 947, 1138, 2182, 2509, 3052, 3317, 3443, 4547
MaeII ACGT 5 cut(s) 159, 1165, 1439, 4114, 4772
MbiI CCGCTC 1 cut(s) 3055
MfeI CAATTG 2 cut(s) 125, 362
MflI RGATCY 5 cut(s) 2020, 2145, 2234, 4637, 4762
MhlI GDGCHC 6 cut(s) 1510, 2506, 2681, 3954, 4270, 4843
MlyI GAGTC 5 cut(s) 479, 640, 1051, 1323, 3995
MmeI TCCRAC 2 cut(s) 1834, 3421
Mph1103I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
MroI TCCGGA 1 cut(s) 4610
MslI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4310
MspA1I CMGCKG 1 cut(s) 3923
MspCI CTTAAG 2 cut(s) 3098, 3488
MspI CCGG 6 cut(s) 58, 69, 89, 2932, 3342, 4611
MspR9I CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4576
MunI CAATTG 2 cut(s) 125, 362
Mva1269I GAATGC 3 cut(s) 906, 2718, 3446
MvaI CCWGG 4 cut(s) 2508, 3050, 3774, 4576
NciI CCSGG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 2072
NheI GCTAGC 2 cut(s) 1481, 3205
NlaIV GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3952
NmuCI GTSAC 3 cut(s) 449, 3389, 4499
NsiI ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
NspI RCATGY 3 cut(s) 2756, 2972, 3543
NspV TTCGAA 2 cut(s) 83, 3516
OliI CACNNNNGTG 1 cut(s) 3360
PaeI GCATGC 2 cut(s) 2756, 3543
PagI TCATGA 1 cut(s) 3331
PctI GAATGC 3 cut(s) 906, 2718, 3446
PfeI GAWTC 9 cut(s) 317, 1034, 1097, 1390, 1676, 1997, 2807, 3869, 4030
PflFI GACNNNGTC 1 cut(s) 59
PfoI TCCNGGA 1 cut(s) 2506
PleI GAGTC 5 cut(s) 478, 639, 1051, 1322, 3994
PpsI GAGTC 5 cut(s) 478, 639, 1051, 1322, 3994
Ppu21I YACGTR 1 cut(s) 4115
PpuMI RGGWCCY 1 cut(s) 2089
PsiI TTATAA 1 cut(s) 767
Psp5II RGGWCCY 1 cut(s) 2089
Psp6I CCWGG 4 cut(s) 2506, 3048, 3772, 4574
PspEI GGTNACC 1 cut(s) 3389
PspGI CCWGG 4 cut(s) 2506, 3048, 3772, 4574
PspN4I GGNNCC 8 cut(s) 1140, 1933, 2022, 2178, 2256, 2505, 2814, 3952
PspOMI GGGCCC 1 cut(s) 3950
PspPPI RGGWCCY 1 cut(s) 2089
PstI CTGCAG 1 cut(s) 4514
PstNI CAGNNNCTG 4 cut(s) 1475, 2749, 3008, 3803
PsuI RGATCY 5 cut(s) 2020, 2145, 2234, 4637, 4762
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 3923
RseI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4310
ScaI AGTACT 2 cut(s) 432, 1757
SchI GAGTC 5 cut(s) 479, 640, 1051, 1323, 3995
ScrFI CCNGG 5 cut(s) 59, 2508, 3050, 3774, 4576
SduI GDGCHC 6 cut(s) 1510, 2506, 2681, 3954, 4270, 4843
SfcI CTRYAG 6 cut(s) 1122, 2149, 2517, 2656, 4510, 4743
SfuI TTCGAA 2 cut(s) 83, 3516
SinI GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4709
SmiMI CAYNNNNRTG 7 cut(s) 464, 531, 743, 1607, 3330, 3360, 4310
SmlI CTYRAG 9 cut(s) 510, 809, 1150, 1274, 2324, 3098, 3488, 4145, 4296
SmoI CTYRAG 9 cut(s) 510, 809, 1150, 1274, 2324, 3098, 3488, 4145, 4296
SphI GCATGC 2 cut(s) 2756, 3543
StyD4I CCNGG 5 cut(s) 57, 2506, 3048, 3772, 4574
StyI CCWWGG 3 cut(s) 322, 2072, 4468
TaiI ACGT 5 cut(s) 162, 1168, 1442, 4117, 4775
TaqI TCGA 8 cut(s) 83, 330, 629, 1871, 2641, 2958, 3516, 4281
TaqII GACCGA 3 cut(s) 2355, 2830, 3831
TatI WGTACW 5 cut(s) 430, 878, 1755, 3652, 4435
TauI GCSGC 4 cut(s) 3058, 4564, 4664, 4832
TfiI GAWTC 9 cut(s) 317, 1034, 1097, 1390, 1676, 1997, 2807, 3869, 4030
TseFI GTSAC 3 cut(s) 449, 3389, 4499
Tsp45I GTSAC 3 cut(s) 449, 3389, 4499
TspGWI ACGGA 2 cut(s) 1525, 3800
Tth111I GACNNNGTC 1 cut(s) 59
Vha464I CTTAAG 2 cut(s) 3098, 3488
VneI GTGCAC 2 cut(s) 1506, 4266
VpaK11BI GGWCC 8 cut(s) 130, 758, 2089, 2254, 2695, 2813, 3843, 4709
XagI CCTNNNNNAGG 1 cut(s) 3884
XbaI TCTAGA 3 cut(s) 1322, 3022, 3554
XceI RCATGY 3 cut(s) 2756, 2972, 3543
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XmiI GTMKAC 5 cut(s) 50, 673, 913, 3231, 3510
ZrmI AGTACT 2 cut(s) 432, 1757
Zsp2I ATGCAT 5 cut(s) 656, 906, 1217, 1534, 3545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.