pycom15g14160

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014543
Physical Location & Seq
Reverse (-)
9644224 .. 9647458
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2901 bp
ATGGAGATTTTCCATTCCTCCTCCTACTTCAACCTTCTCCTCCCTCTTCTCTTCCTCTTCGCGGCGGTTTCCGCCGCCGATATCTACTCAGACACCCTCCTGAGCTTAAAATCCGAGCTCACAGAGGTCAATGGCAGCTTGAAAGATTGGATTCTCCCTTCTGCAGAAAACCCACCTGGCAAAATCCACGCATGTTCTTGGTCCGGCGTCAAATGCAACACCAACAACTCCGCCGTCACTGGCTTAGACCTCTCTATGAAGACTCTCGCCGGTGAAATTTCCGGCAACCAATTCGCCGTCTTCACGGACCTTGTCTCACTCAACCTCAGTTACAACTCGTTCTCCAGTCACCTCCCCGCCGGAATTTTCAACCTCACCAACCTCACAACATTAGACATCAGCAGAAACAACTTCTCCGGCCACTTCCCCGTCGGAGTCTCAGCCCTTCAAAACCTCATTGTTCTCGACGCATTCAGCAACAGCTTTTCCGGGTCGCTACCCGCCGAAATTTCAGAGCTTCAGAACCTCAAAGTCCTCAATTTAGCCGGAAGCTACTTCCGGGGACCGATCCCGGCGGAATACGGCGCTTTCCGCCGCCTTGAGTTTATTCATCTCGCCGGGAATTTGATCACCGGAAGCATTCCGCCGGAACTGGGGAAGCTCAAAACAGTGACGCACATGGAAATCGGCTACAATTATTACCACGGAAGCATTCCGTGGCAATTGGGGAGCATGACGGAACTCCAGTATCTCGACATTGCCTATGCAAATCTCTCCGGCGCCATCCCAAAATCCCTCGGGGATCTCAACAATCTCGAATCCCTCTTCCTCTTCAGAAACCAGCTTTCTGGGTCGATTCCGGCGGAGCTCAGCAACATCAGAGCTCTCCAAAGCTTGGATCTTTCCGACAATCTGATTTCTGGGTCGATCCCACAAACCTTTTCGGAGCTGAAAAACCTTAAACTCCTCAGCCTTTTTTACAACGAGATAGTCGGCGAAGTTCCCGACGGAATTGCAGAGCTTCCATCGCTGGAGACGCTGCTTTTGTGGAACAATTACTTCTCCGGCAACCTTCCCCGGAGCTTGGGGAGAAACTCGATGCTCGGATGGGTGGATGTCTCCACAAACAACCTCAGCGGCGGCGTTCCACCGGATATCTGTGCAAATGGCCAGCTTTTTAAGCTGATGCTGTTTTCGAACAACTTCACCGGCGGCCTCTCCACTTCTCTGTCAAACTGTTCGTCTCTGGTTCGTCTTCGACTCGAAGATAACATGCTTTCAGGTGAGATTCCATTGAAATTCAGCAGTCTGACTGATATCACATATGTTGATTTATCTGGGAACAATTTTAGTGGTGGGATTCCAATGGATATTTCAAAGGCTCAAAAGCTTGAGTACTTCAATGTGTCTAGAAATCCAGAATTAGGAGGCACAGTTCCTGCAGAAATGTGGGAATTGCCAAATCTTCACAATTTTTCCGCTTCCTCCTGTAGTTTGATTGGCAACGTTCCTTCATTCGAAAACTGCAGATCGATATCTGTTGTTGAATTGAGCAAGAACAGTTTGGAAGGAGCTGTTTCGGAAAGTATTTCTAAGTGCCAGGCTTTAGAGAGGATTGATTTAGGGAGCAATAATTTATCCGGTCGTATACCCGAGGAGCTTGCAAGTCTTCCTGCTCTTGGTGTTCTTGACCTGTCACACAATAGCTTCGATGGATCAATACCTAAAAAGTTTGGTTCTTCTTCGAGCTTGGTACTTCTAAATGTGTCTTACAATGATGTTTCCGGAATTATTCCATCTGGAAAGGTATTTAGAACAATGGAACAAAGTGCGTTTGTTGGGAATCCGAAACTTTGTGGAGAGCCTCTGAGATCATGTCCTGGTTCAGTTGCTATATTTGGAAGCAGAGGCGTCGGGAAGCTTTTATGGATTCTGCTACTGTGCGCTGTAGTAGCTATGTTTGTTACTCTATCGGTTATGGGGATATTTTATTTCCGTAGAGAAAGCAAAGGCCAATGGAAGATGATCTCGTTTGATGGACTTCCTCAATTCACAGCAAATGATGTGTTGAAGAGCTTCAGTTCCACAGAATCCACGGATGCATTACCGCCTTTGTCAGCTTCGGTAAGCAAAGCTGTTCTGCCGACCGGAATCACAGTTTCTGTGAAGAAGATTGAATGGGGAGCAAAGAGAATGAGAGTTATGTTGGAATTCGTAACACAAATAGGGAATGCAAGGCATAAGAATTTAATTCGATTGCTCGGAGTTTGCTACAACAATCAACTGGCTTATCTCTTGTACGATTACTTGCCCAATGGAAACTTGGCTGAAAAGATAAGTGTGAAGAGGGAATGGGCAGCCAAGTACAAAATTGTCATCGGTGTTGCAAGGGGATTGCACTTCCTTCACCACGAATGTTATCCTGCAATTGCACATGGAGATCTGAGGTCAAGTAATATAGTGTTTGACGAAAATATGGAACCTCGTTTGACAGAATTCGGGTTCAAGCATCTGCTCGAGTTGAACAAAGATTCGTTTGCAGCAGCAACTTCCACCTGGGATGCAGGTGATAACATCAGTGTCACAAAAGAGGAGCTATGCAGGGATGTATACAGCTTTGGGGAGATCACGCTGCAGATTCTAAGCAGCGGCAGGTTGACAAATTCTGGCGCAAGTATACATAGCAAGTCAAGAGAAGTTGTTCTGCGGGAAATCTACAATGAGAACGAAGCTGGTTCCAACATTTCAATGCAAGAGGAGATAAAACTGGTAGTTGAGGTTGCTATGCTTTGCACCAGGAGTAGGCCATCCGATCGACCATCCATGGAAAGTGCATGGAAGCTTTTATCCGAGTCGAAAATCCAGATGAAAAACAACCCAACTTTTGAAGCAGCAGGGTGA

Protein Analysis

967

Amino Acids

105.45

Weight (kDa)

5.66

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 164 - 280 4.5e-08 Leucine-rich repeat region
LRR_14 PF23598 284 - 352 1.6e-06 Leucine-rich repeat region
LRR_8 PF13855 295 - 353 1.8e-08 Leucine rich repeat
LRR_8 PF13855 511 - 569 7.6e-08 Leucine rich repeat
Pkinase PF00069 708 - 945 9.7e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 716 - 944 5.9e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51350
fragaria_vesca FvH4_1g01520
malus_domestica MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica Prupe.7G253000_v2.0.a1
pyrus_communis pycom02g01130 pycom15g14160
rosa_chinensis RchiOBHm_Chr2g0086441
rosa_laevigata RLG00000015759
rosa_multiflora Rmu_sc0005127.1_g000003
rosa_roxburghii Rroxscaffold_2G00154570
rosa_rugosa Rorug01G0467300
rosa_samantha Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana Rw2G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2555
Acc36I ACCTGC 2 cut(s) 2555, 2643
AccB1I GGYRCC 1 cut(s) 779
AccB7I CCANNNNNTGG 1 cut(s) 895
AccI GTMKAC 3 cut(s) 1648, 2610, 2677
AccII CGCG 1 cut(s) 62
AccIII TCCGGA 1 cut(s) 1784
AclI AACGTT 1 cut(s) 1506
AclWI GGATC 5 cut(s) 562, 810, 906, 922, 1723
AcoI YGGCCR 2 cut(s) 418, 1168
AcsI RAATTY 9 cut(s) 276, 363, 507, 622, 1298, 2210, 2245, 2495, 2662
AcuI CTGAAG 3 cut(s) 503, 817, 2062
AcyI GRCGYC 3 cut(s) 207, 780, 1911
AfaI GTAC 4 cut(s) 1397, 1755, 2300, 2366
AfiI CCNNNNNNNGG 7 cut(s) 61, 653, 895, 1084, 1424, 1679, 2802
AjnI CCWGG 5 cut(s) 175, 1599, 1879, 2555, 2795
AjuI GAANNNNNNNTTGG 2 cut(s) 1357, 1389
AloI GAACNNNNNNTCC 2 cut(s) 1419, 1451
Alw21I GWGCWC 3 cut(s) 120, 870, 886
Alw26I GTCTC 5 cut(s) 319, 442, 1028, 1123, 1248
AlwI GGATC 5 cut(s) 562, 810, 906, 922, 1723
AlwNI CAGNNNCTG 2 cut(s) 1439, 2162
Ama87I CYCGRG 3 cut(s) 797, 1652, 2516
Aor13HI TCCGGA 1 cut(s) 1784
AoxI GGCC 5 cut(s) 418, 1168, 1213, 2011, 2804
ApeKI GCWGC 8 cut(s) 135, 1039, 2357, 2540, 2543, 2632, 2646, 2891
ApoI RAATTY 9 cut(s) 276, 363, 507, 622, 1298, 2210, 2245, 2495, 2662
ArsI GACNNNNNNTTYG 4 cut(s) 1027, 1059, 1226, 1258
Asp700I GAANNNNTTC 3 cut(s) 1202, 2075, 2700
AspLEI GCGC 4 cut(s) 587, 782, 1946, 2672
AspS9I GGNCC 3 cut(s) 201, 307, 563
AsuC2I CCSGG 5 cut(s) 490, 560, 572, 619, 1078
AsuHPI GGTGA 8 cut(s) 284, 341, 367, 622, 1198, 1295, 2399, 2579
AsuII TTCGAA 2 cut(s) 1196, 1518
AvaI CYCGRG 3 cut(s) 797, 1652, 2516
AvaII GGWCC 3 cut(s) 201, 307, 563
BaeI ACNNNNGTAYC 2 cut(s) 731, 764
BalI TGGCCA 1 cut(s) 1170
BanI GGYRCC 1 cut(s) 779
BanII GRGCYC 3 cut(s) 120, 870, 886
BbsI GAAGAC 4 cut(s) 266, 292, 1247, 1661
Bbv12I GWGCWC 3 cut(s) 120, 870, 886
BbvCI CCTCAGC 2 cut(s) 968, 1133
BbvI GCAGC 7 cut(s) 147, 1026, 2369, 2552, 2555, 2619, 2658
BccI CCATC 8 cut(s) 791, 1033, 1101, 1706, 1804, 2030, 2815, 2827
BceAI ACGGC 3 cut(s) 218, 281, 598
BcgI CGANNNNNNTGC 8 cut(s) 274, 308, 995, 1029, 1643, 1677, 1894, 1928
BciT130I CCWGG 5 cut(s) 177, 1601, 1881, 2557, 2797
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 5 cut(s) 490, 560, 572, 619, 1078
BcoDI GTCTC 5 cut(s) 319, 442, 1028, 1123, 1248
BfaI CTAG 1 cut(s) 1410
BfmI CTRYAG 6 cut(s) 162, 1440, 1489, 1525, 1947, 2633
BfoI RGCGCY 2 cut(s) 588, 783
BfuAI ACCTGC 2 cut(s) 2555, 2643
BglII AGATCT 1 cut(s) 2440
BlpI GCTNAGC 1 cut(s) 869
BmcAI AGTACT 1 cut(s) 1397
Bme18I GGWCC 3 cut(s) 201, 307, 563
BmeT110I CYCGRG 3 cut(s) 797, 1652, 2516
BmgT120I GGNCC 3 cut(s) 201, 307, 563
BmiI GGNNCC 4 cut(s) 564, 781, 2481, 2737
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 5 cut(s) 1089, 1176, 2089, 2518, 2551
BmuI ACTGGG 1 cut(s) 662
BpiI GAAGAC 4 cut(s) 266, 292, 1247, 1661
BpmI CTGGAG 3 cut(s) 328, 728, 1052
Bpu10I CCTNAGC 3 cut(s) 101, 968, 1133
Bpu1102I GCTNAGC 1 cut(s) 869
Bpu14I TTCGAA 2 cut(s) 1196, 1518
BpuEI CTTGAG 2 cut(s) 620, 1412
BpuMI CCSGG 5 cut(s) 490, 560, 572, 619, 1078
Bsa29I ATCGAT 1 cut(s) 1532
BsaBI GATNNNNATC 1 cut(s) 1534
BsaHI GRCGYC 3 cut(s) 207, 780, 1911
BsaJI CCNNGG 9 cut(s) 559, 703, 716, 796, 1076, 1653, 2094, 2556, 2823
BsaWI WCCGGW 5 cut(s) 632, 1150, 1640, 1784, 2147
BsaXI ACNNNNNCTCC 6 cut(s) 326, 356, 398, 428, 1419, 1449
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 653, 895, 1084, 1424, 1679, 2802
Bse118I RCCGGY 2 cut(s) 269, 1208
Bse1I ACTGG 6 cut(s) 244, 345, 657, 745, 2289, 2772
Bse3DI GCAATG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 1534
BseAI TCCGGA 1 cut(s) 1784
BseBI CCWGG 5 cut(s) 177, 1601, 1881, 2557, 2797
BseCI ATCGAT 1 cut(s) 1532
BseDI CCNNGG 9 cut(s) 559, 703, 716, 796, 1076, 1653, 2094, 2556, 2823
BseGI GGATG 8 cut(s) 783, 1112, 1120, 2104, 2566, 2611, 2807, 2819
BseJI GATNNNNATC 1 cut(s) 1534
BseLI CCNNNNNNNGG 7 cut(s) 61, 653, 895, 1084, 1424, 1679, 2802
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 9 cut(s) 92, 102, 340, 453, 883, 982, 1147, 1859, 2435
BseNI ACTGG 6 cut(s) 244, 345, 657, 745, 2289, 2772
BseRI GAGGAG 6 cut(s) 10, 29, 956, 1670, 2606, 2771
BseXI GCAGC 7 cut(s) 147, 1026, 2369, 2552, 2555, 2619, 2658
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 3 cut(s) 1645, 2148, 2815
BshFI GGCC 5 cut(s) 420, 1170, 1215, 2013, 2806
BshNI GGYRCC 1 cut(s) 779
BshVI ATCGAT 1 cut(s) 1532
BsiEI CGRYCG 3 cut(s) 1645, 2148, 2815
BsiHKAI GWGCWC 3 cut(s) 120, 870, 886
BsiHKCI CYCGRG 3 cut(s) 797, 1652, 2516
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 7 cut(s) 61, 653, 895, 1084, 1424, 1679, 2802
BsmAI GTCTC 5 cut(s) 319, 442, 1028, 1123, 1248
BsmBI CGTCTC 2 cut(s) 1028, 1248
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 4 cut(s) 470, 639, 711, 2236
BsnI GGCC 5 cut(s) 420, 1170, 1215, 2013, 2806
BsoBI CYCGRG 3 cut(s) 797, 1652, 2516
Bsp119I TTCGAA 2 cut(s) 1196, 1518
Bsp1286I GDGCHC 3 cut(s) 120, 870, 886
Bsp13I TCCGGA 1 cut(s) 1784
Bsp1720I GCTNAGC 1 cut(s) 869
Bsp19I CCATGG 1 cut(s) 2823
BspANI GGCC 5 cut(s) 420, 1170, 1215, 2013, 2806
BspCNI CTCAG 9 cut(s) 93, 101, 339, 452, 882, 981, 1146, 1860, 2436
BspDI ATCGAT 1 cut(s) 1532
BspEI TCCGGA 1 cut(s) 1784
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 4 cut(s) 564, 781, 2481, 2737
BspMAI CTGCAG 4 cut(s) 166, 1444, 1529, 2637
BspMI ACCTGC 2 cut(s) 2555, 2643
BspPI GGATC 5 cut(s) 562, 810, 906, 922, 1723
BspQI GCTCTTC 1 cut(s) 2066
BspT104I TTCGAA 2 cut(s) 1196, 1518
BspT107I GGYRCC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 756
BsrFI RCCGGY 2 cut(s) 269, 1208
BsrI ACTGG 6 cut(s) 244, 345, 657, 745, 2289, 2772
BssAI RCCGGY 2 cut(s) 269, 1208
BssECI CCNNGG 9 cut(s) 559, 703, 716, 796, 1076, 1653, 2094, 2556, 2823
BssNAI GTATAC 3 cut(s) 1649, 2611, 2678
BssNI GRCGYC 3 cut(s) 207, 780, 1911
BssT1I CCWWGG 1 cut(s) 2823
Bst1107I GTATAC 3 cut(s) 1649, 2611, 2678
Bst2UI CCWGG 5 cut(s) 177, 1601, 1881, 2557, 2797
Bst4CI ACNGT 6 cut(s) 670, 1238, 1435, 1562, 1941, 2158
Bst6I CTCTTC 7 cut(s) 51, 56, 62, 830, 836, 2066, 2339
BstACI GRCGYC 3 cut(s) 207, 780, 1911
BstBI TTCGAA 2 cut(s) 1196, 1518
BstC8I GCNNGC 2 cut(s) 1172, 1662
BstDSI CCRYGG 4 cut(s) 703, 716, 2094, 2823
BstF5I GGATG 8 cut(s) 783, 1112, 1120, 2104, 2566, 2611, 2807, 2819
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 2 cut(s) 588, 783
BstHHI GCGC 4 cut(s) 587, 782, 1946, 2672
BstMAI GTCTC 5 cut(s) 319, 442, 1028, 1123, 1248
BstMCI CGRYCG 3 cut(s) 1645, 2148, 2815
BstMWI GCNNNNNNNGC 7 cut(s) 71, 213, 591, 1027, 1036, 1180, 1952
BstNI CCWGG 5 cut(s) 177, 1601, 1881, 2557, 2797
BstNSI RCATGY 2 cut(s) 195, 1276
BstSFI CTRYAG 6 cut(s) 162, 1440, 1489, 1525, 1947, 2633
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 7 cut(s) 147, 1026, 2369, 2552, 2555, 2619, 2658
BstV2I GAAGAC 4 cut(s) 266, 292, 1247, 1661
BstX2I RGATCY 3 cut(s) 802, 898, 2440
BstXI CCANNNNNNTGG 1 cut(s) 848
BstYI RGATCY 3 cut(s) 802, 898, 2440
BstZ17I GTATAC 3 cut(s) 1649, 2611, 2678
Bsu15I ATCGAT 1 cut(s) 1532
BsuRI GGCC 5 cut(s) 420, 1170, 1215, 2013, 2806
BsuTUI ATCGAT 1 cut(s) 1532
BtgI CCRYGG 4 cut(s) 703, 716, 2094, 2823
BtgZI GCGATG 1 cut(s) 1011
BtsCI GGATG 8 cut(s) 783, 1112, 1120, 2104, 2566, 2611, 2807, 2819
BtsIMutI CAGTG 3 cut(s) 237, 675, 2584
BveI ACCTGC 2 cut(s) 2555, 2643
Cac8I GCNNGC 2 cut(s) 1172, 1662
CaiI CAGNNNCTG 2 cut(s) 1439, 2162
CfoI GCGC 4 cut(s) 587, 782, 1946, 2672
Cfr10I RCCGGY 2 cut(s) 269, 1208
Cfr13I GGNCC 3 cut(s) 201, 307, 563
ClaI ATCGAT 1 cut(s) 1532
CseI GACGC 5 cut(s) 196, 476, 682, 1045, 1900
Csp6I GTAC 4 cut(s) 1396, 1754, 2299, 2365
CspCI CAANNNNNGTGG 2 cut(s) 1333, 1368
CviAII CATG 8 cut(s) 192, 679, 733, 1273, 1875, 2435, 2824, 2835
CviQI GTAC 4 cut(s) 1396, 1754, 2299, 2365
DinI GGCGCC 1 cut(s) 781
EaeI YGGCCR 2 cut(s) 418, 1168
Eam1104I CTCTTC 7 cut(s) 51, 56, 62, 830, 836, 2066, 2339
EarI CTCTTC 7 cut(s) 51, 56, 62, 830, 836, 2066, 2339
EciI GGCGGA 6 cut(s) 61, 220, 581, 590, 633, 878
Ecl136II GAGCTC 3 cut(s) 118, 868, 884
Eco130I CCWWGG 1 cut(s) 2823
Eco24I GRGCYC 3 cut(s) 120, 870, 886
Eco32I GATATC 4 cut(s) 82, 1156, 1318, 1536
Eco47I GGWCC 3 cut(s) 201, 307, 563
Eco53kI GAGCTC 3 cut(s) 118, 868, 884
Eco57I CTGAAG 3 cut(s) 503, 817, 2062
Eco88I CYCGRG 3 cut(s) 797, 1652, 2516
EcoICRI GAGCTC 3 cut(s) 118, 868, 884
EcoRI GAATTC 2 cut(s) 2210, 2495
EcoRII CCWGG 5 cut(s) 175, 1599, 1879, 2555, 2795
EcoRV GATATC 4 cut(s) 82, 1156, 1318, 1536
EcoT14I CCWWGG 1 cut(s) 2823
EcoT22I ATGCAT 1 cut(s) 2104
EcoT38I GRGCYC 3 cut(s) 120, 870, 886
EgeI GGCGCC 1 cut(s) 781
EheI GGCGCC 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 2823
Esp3I CGTCTC 2 cut(s) 1028, 1248
FaeI CATG 8 cut(s) 195, 682, 736, 1276, 1878, 2438, 2827, 2838
FaqI GGGAC 1 cut(s) 576
FatI CATG 8 cut(s) 191, 678, 732, 1272, 1874, 2434, 2823, 2834
FauI CCCGC 3 cut(s) 364, 508, 2700
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 3 cut(s) 1648, 2610, 2677
FokI GGATG 8 cut(s) 770, 1119, 1127, 2111, 2573, 2618, 2794, 2806
FriOI GRGCYC 3 cut(s) 120, 870, 886
FspBI CTAG 1 cut(s) 1410
GlaI GCGC 4 cut(s) 586, 781, 1945, 2671
GsuI CTGGAG 3 cut(s) 328, 728, 1052
HaeII RGCGCY 2 cut(s) 588, 783
HaeIII GGCC 5 cut(s) 420, 1170, 1215, 2013, 2806
HgaI GACGC 5 cut(s) 196, 476, 682, 1045, 1900
HhaI GCGC 4 cut(s) 587, 782, 1946, 2672
Hin1I GRCGYC 3 cut(s) 207, 780, 1911
Hin1II CATG 8 cut(s) 195, 682, 736, 1276, 1878, 2438, 2827, 2838
Hin6I GCGC 4 cut(s) 585, 780, 1944, 2670
HinP1I GCGC 4 cut(s) 585, 780, 1944, 2670
HincII GTYRAC 1 cut(s) 2658
HindII GTYRAC 1 cut(s) 2658
HindIII AAGCTT 4 cut(s) 892, 1388, 1919, 2840
HphI GGTGA 8 cut(s) 284, 341, 367, 622, 1198, 1295, 2399, 2579
Hpy166II GTNNAC 4 cut(s) 1649, 2611, 2658, 2678
Hpy8I GTNNAC 4 cut(s) 1649, 2611, 2658, 2678
Hpy99I CGWCG 4 cut(s) 434, 470, 1010, 1916
HpyAV CCTTC 7 cut(s) 44, 168, 455, 1082, 1521, 1562, 2414
HpyCH4III ACNGT 6 cut(s) 670, 1238, 1435, 1562, 1941, 2158
HpyCH4IV ACGT 1 cut(s) 1506
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 213, 591, 1027, 1036, 1180, 1952
HpySE526I ACGT 1 cut(s) 1506
Hsp92I GRCGYC 3 cut(s) 207, 780, 1911
Hsp92II CATG 8 cut(s) 195, 682, 736, 1276, 1878, 2438, 2827, 2838
HspAI GCGC 4 cut(s) 585, 780, 1944, 2670
KasI GGCGCC 1 cut(s) 779
Kpn2I TCCGGA 1 cut(s) 1784
Ksp22I TGATCA 1 cut(s) 627
LguI GCTCTTC 1 cut(s) 2066
LmnI GCTCC 9 cut(s) 729, 865, 946, 1080, 1571, 1626, 1657, 2183, 2593
Lsp1109I GCAGC 7 cut(s) 147, 1026, 2369, 2552, 2555, 2619, 2658
LweI GCATC 5 cut(s) 1089, 1176, 2089, 2518, 2551
MaeI CTAG 1 cut(s) 1410
MaeII ACGT 1 cut(s) 1506
MaeIII GTNAC 8 cut(s) 235, 329, 347, 670, 1695, 1963, 2215, 2581
MfeI CAATTG 2 cut(s) 722, 2427
MflI RGATCY 3 cut(s) 802, 898, 2440
MhlI GDGCHC 3 cut(s) 120, 870, 886
MlsI TGGCCA 1 cut(s) 1170
MluNI TGGCCA 1 cut(s) 1170
Mly113I GGCGCC 1 cut(s) 780
MlyI GAGTC 4 cut(s) 256, 444, 1254, 2861
MmeI TCCRAC 4 cut(s) 412, 930, 2187, 2763
Mox20I TGGCCA 1 cut(s) 1170
Mph1103I ATGCAT 1 cut(s) 2104
MroI TCCGGA 1 cut(s) 1784
MroXI GAANNNNTTC 3 cut(s) 1202, 2075, 2700
MscI TGGCCA 1 cut(s) 1170
MseI TTAA 4 cut(s) 107, 960, 1179, 2249
MslI CAYNNNNRTG 1 cut(s) 2747
Msp20I TGGCCA 1 cut(s) 1170
MspA1I CMGCKG 2 cut(s) 1137, 2649
MunI CAATTG 2 cut(s) 722, 2427
Mva1269I GAATGC 4 cut(s) 470, 639, 711, 2236
MvaI CCWGG 5 cut(s) 177, 1601, 1881, 2557, 2797
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 7 cut(s) 71, 213, 591, 1027, 1036, 1180, 1952
NarI GGCGCC 1 cut(s) 780
NciI CCSGG 5 cut(s) 490, 560, 572, 619, 1078
NcoI CCATGG 1 cut(s) 2823
NdeI CATATG 1 cut(s) 1324
NlaIII CATG 8 cut(s) 195, 682, 736, 1276, 1878, 2438, 2827, 2838
NlaIV GGNNCC 4 cut(s) 564, 781, 2481, 2737
NmuCI GTSAC 5 cut(s) 235, 347, 670, 1695, 2581
NsiI ATGCAT 1 cut(s) 2104
NspI RCATGY 2 cut(s) 195, 1276
NspV TTCGAA 2 cut(s) 1196, 1518
PaeR7I CTCGAG 1 cut(s) 2516
PaqCI CACCTGC 1 cut(s) 2555
PciSI GCTCTTC 1 cut(s) 2066
PctI GAATGC 4 cut(s) 470, 639, 711, 2236
PdmI GAANNNNTTC 3 cut(s) 1202, 2075, 2700
PflFI GACNNNGTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 895
Ple19I CGATCG 1 cut(s) 2815
PleI GAGTC 4 cut(s) 256, 443, 1254, 2860
PluTI GGCGCC 1 cut(s) 783
PpsI GAGTC 4 cut(s) 256, 443, 1254, 2860
Psp124BI GAGCTC 3 cut(s) 120, 870, 886
Psp1406I AACGTT 1 cut(s) 1506
Psp6I CCWGG 5 cut(s) 175, 1599, 1879, 2555, 2795
PspGI CCWGG 5 cut(s) 175, 1599, 1879, 2555, 2795
PspN4I GGNNCC 4 cut(s) 564, 781, 2481, 2737
PspPI GGNCC 3 cut(s) 201, 307, 563
PspXI VCTCGAGB 1 cut(s) 2516
PstI CTGCAG 4 cut(s) 166, 1444, 1529, 2637
PstNI CAGNNNCTG 2 cut(s) 1439, 2162
PsuI RGATCY 3 cut(s) 802, 898, 2440
PsyI GACNNNGTC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 2815
RsaI GTAC 4 cut(s) 1397, 1755, 2300, 2366
RsaNI GTAC 4 cut(s) 1396, 1754, 2299, 2365
RseI CAYNNNNRTG 1 cut(s) 2747
SacI GAGCTC 3 cut(s) 120, 870, 886
SapI GCTCTTC 1 cut(s) 2066
SaqAI TTAA 4 cut(s) 107, 960, 1179, 2249
Sau96I GGNCC 3 cut(s) 201, 307, 563
ScaI AGTACT 1 cut(s) 1397
SchI GAGTC 4 cut(s) 256, 444, 1254, 2861
SduI GDGCHC 3 cut(s) 120, 870, 886
SfaNI GCATC 5 cut(s) 1089, 1176, 2089, 2518, 2551
SfcI CTRYAG 6 cut(s) 162, 1440, 1489, 1525, 1947, 2633
SfoI GGCGCC 1 cut(s) 781
Sfr274I CTCGAG 1 cut(s) 2516
SfuI TTCGAA 2 cut(s) 1196, 1518
SgrAI CRCCGGYG 2 cut(s) 269, 1208
SinI GGWCC 3 cut(s) 201, 307, 563
SlaI CTCGAG 1 cut(s) 2516
SmiMI CAYNNNNRTG 1 cut(s) 2747
SmlI CTYRAG 3 cut(s) 599, 1391, 2516
SmoI CTYRAG 3 cut(s) 599, 1391, 2516
SspDI GGCGCC 1 cut(s) 779
SspMI CTAG 1 cut(s) 1410
SstI GAGCTC 3 cut(s) 120, 870, 886
StyI CCWWGG 1 cut(s) 2823
TaaI ACNGT 6 cut(s) 670, 1238, 1435, 1562, 1941, 2158
TaiI ACGT 1 cut(s) 1509
TaqII GACCGA 1 cut(s) 580
TatI WGTACW 2 cut(s) 1395, 2364
TauI GCSGC 7 cut(s) 65, 77, 597, 1140, 1143, 1215, 2652
Tru1I TTAA 4 cut(s) 107, 960, 1179, 2249
Tru9I TTAA 4 cut(s) 107, 960, 1179, 2249
TscAI CASTG 3 cut(s) 244, 675, 2584
TseFI GTSAC 5 cut(s) 235, 347, 670, 1695, 2581
TseI GCWGC 8 cut(s) 135, 1039, 2357, 2540, 2543, 2632, 2646, 2891
Tsp45I GTSAC 5 cut(s) 235, 347, 670, 1695, 2581
TspDTI ATGAA 4 cut(s) 272, 599, 1503, 2882
TspGWI ACGGA 7 cut(s) 320, 705, 720, 752, 1023, 1985, 2111
TspRI CASTG 3 cut(s) 244, 675, 2584
Tth111I GACNNNGTC 1 cut(s) 311
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 3 cut(s) 201, 307, 563
XapI RAATTY 9 cut(s) 276, 363, 507, 622, 1298, 2210, 2245, 2495, 2662
XbaI TCTAGA 1 cut(s) 1409
XceI RCATGY 2 cut(s) 195, 1276
XcmI CCANNNNNNNNNTGG 1 cut(s) 2320
XhoI CTCGAG 1 cut(s) 2516
XmiI GTMKAC 3 cut(s) 1648, 2610, 2677
XmnI GAANNNNTTC 3 cut(s) 1202, 2075, 2700
XspI CTAG 1 cut(s) 1410
ZrmI AGTACT 1 cut(s) 1397
Zsp2I ATGCAT 1 cut(s) 2104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.