pycom15g18060
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008498
Physical Location & Seq
Forward (+)
12818949 .. 12821776
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGGGTCGCCGAAAGAACACCAACAACAGCAGCAGCAGCAACCAGGGCTCGGGGGCGGGCTTCGGCCACCCCGGCGAAGCCGTAAAAGACGTCCCCGAGAGCTCTAACCCCGACGAGTCCATGTGCGACCCCGAGCTCGACAAGGCCGTCGACGACTCCATGGGTGAGCCGGAGGTCTCATTCGTCGAAGCCGATGGCAAGACCAGCGCTGACTATTACTTCGACTCCTACTCTCACTTCGGTATTCATGAAGAAATGTTGAAAGATTTAGTGAGGACTAAGACATATCAGAATGTTATCTATCAGAATAAGTTTCTAATAAAGGACAAGATTGTTCTTGACGTGGGAGCTGGGACTGGAATTTTGTCCCTCTTTTGTGCAAAAGCGGGCGCAAAGCATGTTTATGCGGTTGAGTGCTCTGACATGGCTGACATGGCAAAAGAGATTGTTGAAACAAACGGATATTCTAATTTAATAACCGTTCTGAAGGGGAAGATTGAAGAAATTGAGCTTCCAGTCCCTAAAGTGGATATTATTATCTCAGAATGGATGGGATATTTTTTGTTGTTTGAGAATATGTTAAATACGGTCCTCTATGCTCGTGATAAATGGCTTGTAGATGATGGAATTCTACTACCAGACAAAACGTCTCTCTTTTTGACAGCTATTGAGGATGCAGAATACAAAGAGGACAAGATAGAATTTTGGAACAATGTTTATGGCTTTGACATGAGTTGCATCAAGAAGCAAGCCATGATGGAGCCCCTTGTGGACACCGTTGACCAAAACCAAATTGTTACAAACAGTCAGCTACTCAAGACAATGGATATATCTAAGATGACTCCAGGAGATGCGTCCTTCACAGCCCCTTTCAAGCTTGTTGCTGAGCGTGATGATTTCATTCATGCTCTTGTAGCCTACTTTGATGTGTCATTTACGAAGTGTCATAAATTGATGGGCTTTTCCACAGGGCCAAGATCACGGTCTACTCACTGGAAGCAAACAGTCCTGTACCTGGAAGATGTGCTAACAGTATGTCAAGGAGAGTCAATTGTCGGAAGCATGACTGTGGCGCAAAACAAAAAGAATCCCCGTGATATTGACATAGTGCTCAAGTATTCGTTAAATGGCCGACGATGCACAGTCTCAAGGGTTCAATGCTACAAAATGCGCTAA

Protein Analysis

392

Amino Acids

43.91

Weight (kDa)

5.06

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 106 - 182 3.6e-09 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 112 - 185 3.7e-08 Methyltransferase domain
Methyltransf_11 PF08241 113 - 197 9.2e-07 Methyltransferase domain
PRMT_C PF22528 214 - 377 6.5e-59 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AatII GACGTC 1 cut(s) 93
AccI GTMKAC 2 cut(s) 150, 986
AciI CCGC 3 cut(s) 56, 386, 407
AcoI YGGCCR 2 cut(s) 64, 1129
AcsI RAATTY 3 cut(s) 360, 627, 701
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 1013
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 3 cut(s) 49, 526, 1015
AgsI TTSAA 5 cut(s) 262, 452, 500, 874, 1157
AhdI GACNNNNNGTC 1 cut(s) 646
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 3 cut(s) 42, 844, 1014
AluBI AGCT 7 cut(s) 102, 136, 350, 511, 665, 811, 877
AluI AGCT 7 cut(s) 102, 136, 350, 511, 665, 811, 877
Alw21I GWGCWC 4 cut(s) 104, 138, 419, 1113
Alw26I GTCTC 3 cut(s) 181, 654, 1150
Ama87I CYCGRG 3 cut(s) 49, 95, 131
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 4 cut(s) 64, 144, 971, 1129
ApeKI GCWGC 3 cut(s) 30, 33, 36
ApoI RAATTY 3 cut(s) 360, 627, 701
ArsI GACNNNNNNTTYG 4 cut(s) 203, 235, 346, 378
AspLEI GCGC 4 cut(s) 209, 392, 1075, 1173
AspS9I GGNCC 2 cut(s) 589, 971
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 176
AvaI CYCGRG 3 cut(s) 49, 95, 131
AvaII GGWCC 1 cut(s) 589
BanII GRGCYC 4 cut(s) 50, 104, 138, 765
BauI CACGAG 1 cut(s) 600
Bbv12I GWGCWC 4 cut(s) 104, 138, 419, 1113
BbvI GCAGC 3 cut(s) 42, 45, 48
BccI CCATC 5 cut(s) 188, 544, 617, 751, 949
BceAI ACGGC 2 cut(s) 65, 131
BciT130I CCWGG 3 cut(s) 44, 846, 1016
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 3 cut(s) 181, 654, 1150
BfoI RGCGCY 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 3 cut(s) 31, 34, 37
BlpI GCTNAGC 1 cut(s) 885
BlsI GCNGC 3 cut(s) 32, 35, 38
Bme1390I CCNGG 4 cut(s) 44, 72, 846, 1016
Bme18I GGWCC 1 cut(s) 589
BmeRI GACNNNNNGTC 1 cut(s) 646
BmeT110I CYCGRG 3 cut(s) 49, 95, 131
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 589, 971
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 4 cut(s) 44, 72, 846, 1016
BmsI GCATC 4 cut(s) 664, 747, 841, 1127
BpmI CTGGAG 1 cut(s) 828
Bpu1102I GCTNAGC 1 cut(s) 885
BpuEI CTTGAG 3 cut(s) 800, 1097, 1132
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 3 cut(s) 43, 70, 159
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 526, 1015
Bse1I ACTGG 3 cut(s) 361, 515, 998
BseBI CCWGG 3 cut(s) 44, 846, 1016
BseDI CCNNGG 3 cut(s) 43, 70, 159
BseGI GGATG 2 cut(s) 555, 679
BseLI CCNNNNNNNGG 3 cut(s) 49, 526, 1015
BseMII CTCAG 2 cut(s) 555, 876
BseNI ACTGG 3 cut(s) 361, 515, 998
BseXI GCAGC 3 cut(s) 42, 45, 48
BseYI CCCAGC 1 cut(s) 350
BshFI GGCC 4 cut(s) 66, 146, 973, 1131
BsiHKAI GWGCWC 4 cut(s) 104, 138, 419, 1113
BsiHKCI CYCGRG 3 cut(s) 49, 95, 131
BsiSI CCGG 2 cut(s) 72, 170
BslFI GGGAC 4 cut(s) 77, 352, 367, 503
BslI CCNNNNNNNGG 3 cut(s) 49, 526, 1015
BsmAI GTCTC 3 cut(s) 181, 654, 1150
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 4 cut(s) 77, 352, 367, 503
BsnI GGCC 4 cut(s) 66, 146, 973, 1131
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 3 cut(s) 49, 95, 131
Bsp1286I GDGCHC 6 cut(s) 50, 104, 138, 419, 765, 1113
Bsp143I GATC 1 cut(s) 977
Bsp1720I GCTNAGC 1 cut(s) 885
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 3 cut(s) 56, 386, 407
BspANI GGCC 4 cut(s) 66, 146, 973, 1131
BspCNI CTCAG 2 cut(s) 554, 877
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 762
BspTNI GGTCTC 1 cut(s) 181
BsrI ACTGG 3 cut(s) 361, 515, 998
BssECI CCNNGG 3 cut(s) 43, 70, 159
BssMI GATC 1 cut(s) 977
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 3 cut(s) 44, 846, 1016
Bst4CI ACNGT 9 cut(s) 481, 589, 778, 806, 984, 1006, 1033, 1069, 1144
BstACI GRCGYC 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 58, 388, 750
BstDEI CTNAG 4 cut(s) 279, 541, 834, 885
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 555, 679
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 4 cut(s) 209, 392, 1075, 1173
BstKTI GATC 1 cut(s) 980
BstMAI GTCTC 3 cut(s) 181, 654, 1150
BstMBI GATC 1 cut(s) 977
BstMWI GCNNNNNNNGC 7 cut(s) 36, 45, 72, 204, 434, 914, 1137
BstNI CCWGG 3 cut(s) 44, 846, 1016
BstNSI RCATGY 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 42, 70, 844, 1014
BstV1I GCAGC 3 cut(s) 42, 45, 48
BsuRI GGCC 4 cut(s) 66, 146, 973, 1131
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 2 cut(s) 555, 679
BtsIMutI CAGTG 1 cut(s) 991
Cac8I GCNNGC 3 cut(s) 58, 388, 750
CciI TCATGA 1 cut(s) 247
CfoI GCGC 4 cut(s) 209, 392, 1075, 1173
Cfr13I GGNCC 2 cut(s) 589, 971
CseI GACGC 1 cut(s) 843
Csp6I GTAC 1 cut(s) 1012
CviQI GTAC 1 cut(s) 1012
DdeI CTNAG 4 cut(s) 279, 541, 834, 885
DpnI GATC 1 cut(s) 979
DpnII GATC 1 cut(s) 977
DrdI GACNNNNNNGTC 1 cut(s) 146
DriI GACNNNNNGTC 1 cut(s) 646
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 2 cut(s) 64, 1129
Eam1105I GACNNNNNGTC 1 cut(s) 646
Ecl136II GAGCTC 2 cut(s) 102, 136
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 4 cut(s) 50, 104, 138, 765
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 589
Eco47III AGCGCT 1 cut(s) 208
Eco53kI GAGCTC 2 cut(s) 102, 136
Eco57I CTGAAG 1 cut(s) 506
Eco88I CYCGRG 3 cut(s) 49, 95, 131
EcoICRI GAGCTC 2 cut(s) 102, 136
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 3 cut(s) 42, 844, 1014
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 4 cut(s) 50, 104, 138, 765
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 654
FaqI GGGAC 4 cut(s) 77, 352, 367, 503
FauI CCCGC 2 cut(s) 49, 379
FblI GTMKAC 2 cut(s) 150, 986
Fnu4HI GCNGC 3 cut(s) 31, 34, 37
FokI GGATG 2 cut(s) 562, 686
FriOI GRGCYC 4 cut(s) 50, 104, 138, 765
Fsp4HI GCNGC 3 cut(s) 31, 34, 37
GlaI GCGC 4 cut(s) 208, 391, 1074, 1172
GluI GCNGC 3 cut(s) 31, 34, 37
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 828
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 4 cut(s) 66, 146, 973, 1131
HapII CCGG 2 cut(s) 72, 170
HgaI GACGC 1 cut(s) 843
HhaI GCGC 4 cut(s) 209, 392, 1075, 1173
Hin1I GRCGYC 1 cut(s) 90
Hin6I GCGC 4 cut(s) 207, 390, 1073, 1171
HinP1I GCGC 4 cut(s) 207, 390, 1073, 1171
HincII GTYRAC 2 cut(s) 151, 781
HindII GTYRAC 2 cut(s) 151, 781
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 6 cut(s) 116, 155, 224, 841, 1046, 1087
HpaII CCGG 2 cut(s) 72, 170
HphI GGTGA 1 cut(s) 176
Hpy166II GTNNAC 4 cut(s) 151, 772, 781, 987
Hpy188I TCNGA 6 cut(s) 291, 306, 421, 486, 544, 1058
Hpy188III TCNNGA 5 cut(s) 248, 338, 602, 742, 817
Hpy8I GTNNAC 4 cut(s) 151, 772, 781, 987
Hpy99I CGWCG 5 cut(s) 116, 152, 155, 188, 1137
HpyAV CCTTC 2 cut(s) 481, 868
HpyCH4III ACNGT 9 cut(s) 481, 589, 778, 806, 984, 1006, 1033, 1069, 1144
HpyCH4IV ACGT 3 cut(s) 90, 342, 647
HpyCH4V TGCA 4 cut(s) 380, 677, 738, 1140
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 45, 72, 204, 434, 914, 1137
HpyF3I CTNAG 4 cut(s) 279, 541, 834, 885
HpySE526I ACGT 3 cut(s) 90, 342, 647
Hsp92I GRCGYC 1 cut(s) 90
HspAI GCGC 4 cut(s) 207, 390, 1073, 1171
Kzo9I GATC 1 cut(s) 977
LmnI GCTCC 2 cut(s) 347, 760
Lsp1109I GCAGC 3 cut(s) 42, 45, 48
LweI GCATC 4 cut(s) 664, 747, 841, 1127
MaeII ACGT 3 cut(s) 90, 342, 647
MaeIII GTNAC 1 cut(s) 796
MalI GATC 1 cut(s) 979
MboI GATC 1 cut(s) 977
MboII GAAGA 4 cut(s) 263, 505, 512, 1031
MfeI CAATTG 1 cut(s) 1050
MhlI GDGCHC 6 cut(s) 50, 104, 138, 419, 765, 1113
MluCI AATT 8 cut(s) 360, 469, 504, 627, 701, 792, 950, 1050
MlyI GAGTC 5 cut(s) 125, 149, 218, 835, 1055
MmeI TCCRAC 1 cut(s) 1036
MnlI CCTC 6 cut(s) 166, 267, 380, 602, 664, 682
MseI TTAA 3 cut(s) 473, 581, 1124
MslI CAYNNNNRTG 2 cut(s) 402, 1067
MspI CCGG 2 cut(s) 72, 170
MspR9I CCNGG 4 cut(s) 44, 72, 846, 1016
MunI CAATTG 1 cut(s) 1050
MvaI CCWGG 3 cut(s) 44, 846, 1016
MwoI GCNNNNNNNGC 7 cut(s) 36, 45, 72, 204, 434, 914, 1137
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 1 cut(s) 159
NdeII GATC 1 cut(s) 977
NlaIV GGNNCC 1 cut(s) 762
NspI RCATGY 1 cut(s) 401
PagI TCATGA 1 cut(s) 247
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 189
PfeI GAWTC 1 cut(s) 1087
PfoI TCCNGGA 1 cut(s) 844
PkrI GCNGC 3 cut(s) 32, 35, 38
PleI GAGTC 5 cut(s) 124, 149, 218, 835, 1054
PpsI GAGTC 5 cut(s) 124, 149, 218, 835, 1054
Psp124BI GAGCTC 2 cut(s) 104, 138
Psp6I CCWGG 3 cut(s) 42, 844, 1014
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 3 cut(s) 42, 844, 1014
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 589, 971
RsaI GTAC 1 cut(s) 1013
RsaNI GTAC 1 cut(s) 1012
RseI CAYNNNNRTG 2 cut(s) 402, 1067
SacI GAGCTC 2 cut(s) 104, 138
SalI GTCGAC 1 cut(s) 149
SaqAI TTAA 3 cut(s) 473, 581, 1124
SatI GCNGC 3 cut(s) 31, 34, 37
Sau3AI GATC 1 cut(s) 977
Sau96I GGNCC 2 cut(s) 589, 971
SchI GAGTC 5 cut(s) 125, 149, 218, 835, 1055
ScrFI CCNGG 4 cut(s) 44, 72, 846, 1016
SduI GDGCHC 6 cut(s) 50, 104, 138, 419, 765, 1113
SfaNI GCATC 4 cut(s) 664, 747, 841, 1127
SgrDI CGTCGACG 1 cut(s) 149
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 402, 1067
SmlI CTYRAG 3 cut(s) 815, 1112, 1147
SmoI CTYRAG 3 cut(s) 815, 1112, 1147
Sse9I AATT 8 cut(s) 360, 469, 504, 627, 701, 792, 950, 1050
SsiI CCGC 3 cut(s) 56, 386, 407
SstI GAGCTC 2 cut(s) 104, 138
StyD4I CCNGG 4 cut(s) 42, 70, 844, 1014
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 9 cut(s) 481, 589, 778, 806, 984, 1006, 1033, 1069, 1144
TaiI ACGT 3 cut(s) 93, 345, 650
TaqI TCGA 4 cut(s) 138, 150, 186, 222
TasI AATT 8 cut(s) 360, 469, 504, 627, 701, 792, 950, 1050
TfiI GAWTC 1 cut(s) 1087
Tru1I TTAA 3 cut(s) 473, 581, 1124
Tru9I TTAA 3 cut(s) 473, 581, 1124
TscAI CASTG 1 cut(s) 998
TseI GCWGC 3 cut(s) 30, 33, 36
TspDTI ATGAA 4 cut(s) 236, 264, 889, 893
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 1 cut(s) 998
VpaK11BI GGWCC 1 cut(s) 589
XapI RAATTY 3 cut(s) 360, 627, 701
XceI RCATGY 1 cut(s) 401
XmiI GTMKAC 2 cut(s) 150, 986
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.