pycom15g18220

ubiE/COQ5 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007355
Physical Location & Seq
Reverse (-)
12916417 .. 12917982
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGCTGGACATGTGTAGCAGTTGGGTCAGTCACTTTCCACTGGGATACAAGCAAGAGCGAATAGTGGGAATGGGGATGAATGAAGAAGAGCTAAAGCGCAATCCGGTCTTGACAGAGTATGTTGTGCAAGACTTGAATGTGAACCCCAAACTTCCCTTTGAAGACAATTCTTTTGATGTGATTACTAATGTGGTTAGCGTTGATTACATCACAAAGCCTCTTGACGTTTTCAAGGAAATGTGCCGGATTCTTAAGCCAGGTGGACTTGCTATAATGAGCTTTTCCAACCGTTGTTTCTGGACTAAAGCAATCTCAATATGGACATCAACCGGTGATGCTGATCATGGTTTGATTGTGGGGGCATATTTCCACTACGCTGGAGGATTTGAACCACCTCAGGCGGTGGATATATCCCCGAACCCTGGACGCTCAGATCCTATGTACATTGTATACTCTAGAAAATTATCTACGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

159

Amino Acids

17.8

Weight (kDa)

5.3

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 28 - 91 7.4e-12 Methyltransferase domain
Methyltransf_25 PF13649 30 - 88 5.1e-07 Methyltransferase domain
Methyltransf_31 PF13847 42 - 95 2.4e-06 Methyltransferase domain
Ubie_methyltran PF01209 48 - 96 1.6e-06 ubiE/COQ5 methyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 450
AciI CCGC 1 cut(s) 401
AclWI GGATC 1 cut(s) 428
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 422
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 9
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 4 cut(s) 136, 161, 232, 389
AjnI CCWGG 2 cut(s) 256, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 2 cut(s) 91, 279
AluI AGCT 2 cut(s) 91, 279
AlwI GGATC 1 cut(s) 428
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AsiGI ACCGGT 1 cut(s) 329
AspLEI GCGC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 344
AxyI CCTNAGG 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 168
BciT130I CCWGG 2 cut(s) 258, 423
BciVI GTATCC 1 cut(s) 38
BclI TGATCA 1 cut(s) 340
BfaI CTAG 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 251
BfuI GTATCC 1 cut(s) 38
Bme1390I CCNGG 2 cut(s) 258, 423
BmrFI CCNGG 2 cut(s) 258, 423
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 325
BmuI ACTGGG 1 cut(s) 50
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 421
BsaWI WCCGGW 2 cut(s) 103, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 45
Bse21I CCTNAGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 2 cut(s) 258, 423
BseDI CCNNGG 1 cut(s) 421
BseGI GGATG 1 cut(s) 81
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 422
BseMII CTCAG 2 cut(s) 410, 444
BseNI ACTGG 1 cut(s) 45
BshTI ACCGGT 1 cut(s) 329
BsiSI CCGG 3 cut(s) 104, 244, 330
BslI CCNNNNNNNGG 1 cut(s) 422
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 2 cut(s) 340, 433
BspACI CCGC 1 cut(s) 401
BspCNI CTCAG 2 cut(s) 409, 443
BspPI GGATC 1 cut(s) 428
BspQI GCTCTTC 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 329
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 421
BssMI GATC 2 cut(s) 340, 433
BssNAI GTATAC 1 cut(s) 451
Bst1107I GTATAC 1 cut(s) 451
Bst2UI CCWGG 2 cut(s) 258, 423
Bst4CI ACNGT 2 cut(s) 290, 472
Bst6I CTCTTC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 441
BstDEI CTNAG 2 cut(s) 396, 430
BstF5I GGATG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 343, 436
BstMBI GATC 2 cut(s) 340, 433
BstNI CCWGG 2 cut(s) 258, 423
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 2 cut(s) 256, 421
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 377
BstYI RGATCY 1 cut(s) 433
BstZ17I GTATAC 1 cut(s) 451
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 1 cut(s) 329
CseI GACGC 1 cut(s) 435
Csp6I GTAC 1 cut(s) 442
CspAI ACCGGT 1 cut(s) 329
CviAII CATG 2 cut(s) 10, 344
CviJI RGCY 4 cut(s) 91, 217, 256, 279
CviKI_1 RGCY 4 cut(s) 91, 217, 256, 279
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 396, 430
DpnI GATC 2 cut(s) 342, 435
DpnII GATC 2 cut(s) 340, 433
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco81I CCTNAGG 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 256, 421
FaeI CATG 2 cut(s) 13, 347
FatI CATG 2 cut(s) 9, 343
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 450
FokI GGATG 1 cut(s) 88
FspBI CTAG 1 cut(s) 456
GlaI GCGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 399
HapII CCGG 3 cut(s) 104, 244, 330
HgaI GACGC 1 cut(s) 435
HhaI GCGC 1 cut(s) 99
Hin1II CATG 2 cut(s) 13, 347
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 1 cut(s) 247
HpaII CCGG 3 cut(s) 104, 244, 330
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 142, 263, 451
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 4 cut(s) 109, 221, 298, 456
Hpy8I GTNNAC 3 cut(s) 142, 263, 451
HpyCH4III ACNGT 2 cut(s) 290, 472
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 127
HpyF3I CTNAG 2 cut(s) 396, 430
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 2 cut(s) 13, 347
HspAI GCGC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 2 cut(s) 340, 433
LguI GCTCTTC 1 cut(s) 81
LweI GCATC 1 cut(s) 325
MaeI CTAG 1 cut(s) 456
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 342, 435
MboI GATC 2 cut(s) 340, 433
MboII GAAGA 3 cut(s) 95, 98, 173
MflI RGATCY 1 cut(s) 433
MluCI AATT 2 cut(s) 166, 461
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 3 cut(s) 228, 374, 405
MseI TTAA 1 cut(s) 252
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 3 cut(s) 104, 244, 330
MspR9I CCNGG 2 cut(s) 258, 423
MvaI CCWGG 2 cut(s) 258, 423
NdeII GATC 2 cut(s) 340, 433
NlaIII CATG 2 cut(s) 13, 347
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 13
PciI ACATGT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 81
PfeI GAWTC 1 cut(s) 247
PinAI ACCGGT 1 cut(s) 329
PscI ACATGT 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 256, 421
PspGI CCWGG 2 cut(s) 256, 421
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 252
Sau3AI GATC 2 cut(s) 340, 433
ScrFI CCNGG 2 cut(s) 258, 423
SetI ASST 5 cut(s) 93, 228, 262, 281, 397
SfaNI GCATC 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 166, 461
SsiI CCGC 1 cut(s) 401
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 256, 421
TaaI ACNGT 2 cut(s) 290, 472
TaiI ACGT 1 cut(s) 228
TasI AATT 2 cut(s) 166, 461
TatI WGTACW 1 cut(s) 441
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 2 cut(s) 92, 96
TspRI CASTG 1 cut(s) 45
Vha464I CTTAAG 1 cut(s) 251
XbaI TCTAGA 1 cut(s) 455
XceI RCATGY 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.