pycom15g21220

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007936
Physical Location & Seq
Reverse (-)
15227823 .. 15229716
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGGCCTTTAAAAATTGGTTTTGCCTACATTTGACAGAGGCTTCTCCAACCCATTTCGTTCCCTTACCAGACTTACGCTGCTGCGGCAGCAACTACCTAGTTATGGAAGGAATTGACTCGCCCGATATGTCGACGGTATCGCGTCATCGTTTCGGGGACGACTCGGAAGAACCGGCCTTCTCCGTCTCCATCATCGAGAATATGAAGGAAGAGTACGGGCTCTTCGTGTGGCCCTGCAGCTTAGCCCTGGCGGAGTATGTTTGGCAACAGAGATTGCGCTTTTCGGGAGCTAGCGTAGTCGAGCTTGGCGCTGGAACTTCCTTGCCCGGTCTGGTTGCCGCCAGAGTTGGTGCTGACGTCACTCTCACTGATGATTCGAGTAGATTGGAGGTGCTGGACAACATGAGAAGAGTGCTCGACCTTAATAAACTTGAGTGCAATGTAATGGGATTGACATGGGGAGCTTGGGATGCATCAACGTTCAGCCTACACCCCAAATTTATTCTCGGGGCTGATGTATTGTATGATGCTAGTGCCTTTGATGACCTCTTTGCCACCGTGACATTTCTGCTCCGAAATTCTCCAGGGTCAGTCTTCATAACAACATACCATAATCGAAGCGGGCATCATCTTATTGAATTCTTGATGGTCAAATGGGGATTGAAGTGCTTGAAGCTTCTTGATGCCTTTTCATTTATGCCATCCTACAAGGCATCCAGGCTAAGTGGCAACCTTCAACTGGCAGAGATCGTTCTGGATAGCGAACAGGCTGAGACAACGGTTTTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

264

Amino Acids

29.31

Weight (kDa)

5.03

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 62 - 207 1.1e-19 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
AccI GTMKAC 1 cut(s) 131
AccII CGCG 1 cut(s) 142
AciI CCGC 4 cut(s) 84, 251, 339, 621
AclI AACGTT 1 cut(s) 479
AcsI RAATTY 3 cut(s) 497, 577, 638
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 103, 739
AgsI TTSAA 4 cut(s) 638, 664, 673, 737
AjnI CCWGG 3 cut(s) 246, 583, 716
AluBI AGCT 5 cut(s) 240, 290, 304, 464, 676
AluI AGCT 5 cut(s) 240, 290, 304, 464, 676
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 190, 767
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 3 cut(s) 3, 174, 230
ApeKI GCWGC 4 cut(s) 78, 81, 87, 237
ApoI RAATTY 3 cut(s) 497, 577, 638
ArsI GACNNNNNNTTYG 1 cut(s) 766
AspLEI GCGC 2 cut(s) 279, 311
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 327
AsuNHI GCTAGC 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 506
BanII GRGCYC 1 cut(s) 222
BarI GAAGNNNNNNTAC 4 cut(s) 197, 206, 229, 238
BbsI GAAGAC 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 4 cut(s) 65, 68, 99, 249
BccI CCATC 3 cut(s) 197, 640, 709
BciT130I CCWGG 3 cut(s) 248, 585, 718
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 2 cut(s) 190, 767
BfaI CTAG 3 cut(s) 98, 291, 531
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 6 cut(s) 79, 82, 85, 88, 238, 339
BlpI GCTNAGC 1 cut(s) 241
BlsI GCNGC 6 cut(s) 80, 83, 86, 89, 239, 340
Bme1390I CCNGG 4 cut(s) 248, 327, 585, 718
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 231
BmrFI CCNGG 4 cut(s) 248, 327, 585, 718
BmsI GCATC 6 cut(s) 460, 482, 517, 634, 673, 722
BmtI GCTAGC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 586
BpmI CTGGAG 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 246, 584
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 739
Bse118I RCCGGY 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 744
Bse3DI GCAATG 1 cut(s) 445
BseBI CCWGG 3 cut(s) 248, 585, 718
BseDI CCNNGG 2 cut(s) 246, 584
BseGI GGATG 3 cut(s) 475, 701, 713
BseLI CCNNNNNNNGG 2 cut(s) 103, 739
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 1 cut(s) 744
BseXI GCAGC 4 cut(s) 65, 68, 99, 249
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 3 cut(s) 5, 176, 232
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 2 cut(s) 173, 327
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 103, 739
BsmAI GTCTC 2 cut(s) 190, 767
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 3 cut(s) 5, 176, 232
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 222, 417
Bsp143I GATC 1 cut(s) 747
Bsp1720I GCTNAGC 1 cut(s) 241
BspACI CCGC 4 cut(s) 84, 251, 339, 621
BspANI GGCC 3 cut(s) 5, 176, 232
BspCNI CTCAG 1 cut(s) 763
BspFNI CGCG 1 cut(s) 142
BspMAI CTGCAG 1 cut(s) 239
BspOI GCTAGC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 172
BsrI ACTGG 1 cut(s) 744
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 246, 584
BssMI GATC 1 cut(s) 747
BssNI GRCGYC 1 cut(s) 357
Bst2UI CCWGG 3 cut(s) 248, 585, 718
Bst4CI ACNGT 3 cut(s) 136, 559, 781
Bst6I CTCTTC 3 cut(s) 204, 227, 403
BstACI GRCGYC 1 cut(s) 357
BstC8I GCNNGC 2 cut(s) 292, 623
BstDEI CTNAG 3 cut(s) 241, 722, 771
BstF5I GGATG 3 cut(s) 475, 701, 713
BstFNI CGCG 1 cut(s) 142
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 279, 311
BstKTI GATC 1 cut(s) 750
BstMAI GTCTC 2 cut(s) 190, 767
BstMBI GATC 1 cut(s) 747
BstMWI GCNNNNNNNGC 3 cut(s) 84, 87, 470
BstNI CCWGG 3 cut(s) 248, 585, 718
BstSCI CCNGG 4 cut(s) 246, 325, 583, 716
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 4 cut(s) 65, 68, 99, 249
BstV2I GAAGAC 1 cut(s) 586
BsuRI GGCC 3 cut(s) 5, 176, 232
BtsCI GGATG 3 cut(s) 475, 701, 713
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 292, 623
CfoI GCGC 2 cut(s) 279, 311
Cfr10I RCCGGY 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 403, 456
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 3 cut(s) 241, 722, 771
DpnI GATC 1 cut(s) 749
DpnII GATC 1 cut(s) 747
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 3 cut(s) 204, 227, 403
EarI CTCTTC 3 cut(s) 204, 227, 403
EciI GGCGGA 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 506
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 246, 583, 716
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 2 cut(s) 406, 459
FaqI GGGAC 1 cut(s) 170
FatI CATG 2 cut(s) 402, 455
FauI CCCGC 1 cut(s) 614
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 6 cut(s) 79, 82, 85, 88, 238, 339
FokI GGATG 3 cut(s) 482, 688, 700
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 6 cut(s) 79, 82, 85, 88, 238, 339
FspBI CTAG 3 cut(s) 98, 291, 531
GlaI GCGC 2 cut(s) 278, 310
GluI GCNGC 6 cut(s) 79, 82, 85, 88, 238, 339
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 3 cut(s) 5, 176, 232
HapII CCGG 2 cut(s) 173, 327
HgaI GACGC 1 cut(s) 131
HhaI GCGC 2 cut(s) 279, 311
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 2 cut(s) 406, 459
Hin6I GCGC 2 cut(s) 277, 309
HinP1I GCGC 2 cut(s) 277, 309
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 3 cut(s) 116, 161, 374
HpaII CCGG 2 cut(s) 173, 327
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 2 cut(s) 166, 575
Hpy188III TCNNGA 5 cut(s) 196, 285, 643, 680, 755
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 4 cut(s) 101, 187, 199, 743
HpyCH4III ACNGT 3 cut(s) 136, 559, 781
HpyCH4IV ACGT 2 cut(s) 357, 479
HpyCH4V TGCA 4 cut(s) 237, 438, 473, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 87, 470
HpyF3I CTNAG 3 cut(s) 241, 722, 771
HpySE526I ACGT 2 cut(s) 357, 479
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 2 cut(s) 406, 459
HspAI GCGC 2 cut(s) 277, 309
Kzo9I GATC 1 cut(s) 747
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 3 cut(s) 287, 461, 576
Lsp1109I GCAGC 4 cut(s) 65, 68, 99, 249
LweI GCATC 6 cut(s) 460, 482, 517, 634, 673, 722
MaeI CTAG 3 cut(s) 98, 291, 531
MaeII ACGT 2 cut(s) 357, 479
MaeIII GTNAC 2 cut(s) 358, 559
MalI GATC 1 cut(s) 749
MboI GATC 1 cut(s) 747
MboII GAAGA 5 cut(s) 179, 214, 221, 420, 586
MhlI GDGCHC 2 cut(s) 222, 417
MluCI AATT 5 cut(s) 13, 111, 497, 577, 638
MlyI GAGTC 2 cut(s) 110, 155
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 3 cut(s) 31, 382, 557
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 2 cut(s) 9, 423
MspI CCGG 2 cut(s) 173, 327
MspR9I CCNGG 4 cut(s) 248, 327, 585, 718
MvaI CCWGG 3 cut(s) 248, 585, 718
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 3 cut(s) 84, 87, 470
NciI CCSGG 1 cut(s) 327
NdeII GATC 1 cut(s) 747
NheI GCTAGC 1 cut(s) 290
NlaIII CATG 2 cut(s) 406, 459
NmuCI GTSAC 2 cut(s) 358, 559
NsiI ATGCAT 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 6 cut(s) 80, 83, 86, 89, 239, 340
PleI GAGTC 2 cut(s) 110, 155
PpsI GAGTC 2 cut(s) 110, 155
Psp1406I AACGTT 1 cut(s) 479
Psp6I CCWGG 3 cut(s) 246, 583, 716
PspGI CCWGG 3 cut(s) 246, 583, 716
PspPI GGNCC 1 cut(s) 231
PstI CTGCAG 1 cut(s) 239
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SalI GTCGAC 1 cut(s) 130
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 9, 423
SatI GCNGC 6 cut(s) 79, 82, 85, 88, 238, 339
Sau3AI GATC 1 cut(s) 747
Sau96I GGNCC 1 cut(s) 231
SchI GAGTC 2 cut(s) 110, 155
ScrFI CCNGG 4 cut(s) 248, 327, 585, 718
SduI GDGCHC 2 cut(s) 222, 417
SfaNI GCATC 6 cut(s) 460, 482, 517, 634, 673, 722
SfcI CTRYAG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 5 cut(s) 13, 111, 497, 577, 638
SsiI CCGC 4 cut(s) 84, 251, 339, 621
SspMI CTAG 3 cut(s) 98, 291, 531
StyD4I CCNGG 4 cut(s) 246, 325, 583, 716
TaaI ACNGT 3 cut(s) 136, 559, 781
TaiI ACGT 2 cut(s) 360, 482
TaqI TCGA 6 cut(s) 131, 195, 300, 377, 417, 616
TasI AATT 5 cut(s) 13, 111, 497, 577, 638
TauI GCSGC 2 cut(s) 87, 341
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 2 cut(s) 9, 423
Tru9I TTAA 2 cut(s) 9, 423
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 2 cut(s) 358, 559
TseI GCWGC 4 cut(s) 78, 81, 87, 237
Tsp45I GTSAC 2 cut(s) 358, 559
TspDTI ATGAA 3 cut(s) 218, 586, 681
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 1 cut(s) 373
XapI RAATTY 3 cut(s) 497, 577, 638
XmiI GTMKAC 1 cut(s) 131
XspI CTAG 3 cut(s) 98, 291, 531
ZraI GACGTC 1 cut(s) 358
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.