pycom15g21480

Receptor homology region, transmembrane domain- and RING domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008527
Physical Location & Seq
Reverse (-)
15420679 .. 15422842
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 876 bp
ATGGGGCAAGCTGTAGTGGGATTCTCTGTGATTGCTTGCTTTTTCCTTCTCCAACTAAGCACTGCTAATGTTGTTCTCCAACCATCTGGAATTCTTGTCGCCGACCTTCCCGCCAAATTTGCTGGTGCTGTCAGTAGCTCTGAAATATGTGGAGTTTTGCTTGTGTCCGACCCTTTGCATGCCTGCTCGCCTCTACGCAATGGTCTTCAAGCAAGTGAAACCGATATAACGAGGTTTGCTTTAGTAGAAAGAGGTGAATGCCCTTTCGAGGATAAAGTGCAACACGCTCAAAATGCCGGATTTCGTGCTGTGATTGTTTATGATGATAGGAAAGAAAAATTGGTTCACATGACGATAAACAGCAAGAATGTTACAATTCGTTCTGTATTTGTTTCGAAAGCATCTGGTGAAATGCTAAAGGAGCATGCTCGTGGGGAAGAAGGGGAATGCTGCATCTTCCTATTGCCAAATGAAAAGGCCTGGTTGGTGTTTGCTGTATCGATCTCATTTCTAACCGTATTGGGTCTCCTTATAATTGCCTGCTGTGCTCAGAGAGACTGGCGCTGGCCCTATTCTCAAGGAAGAAACCAGCTTCCTAAAAGTGCGGATACCGAGATTGTTGATGCTCTCCCTTGCTTCACATTCAGTTCTGCTGGTTTGGATGAATCCCACATTGAAGAAACTTGCGCCATCTGCCTTGAGAATTACAAAGATTCCGAAATTCTTAAAGTTCTTCCCTGCCAGCATGAATTTCATTCAAGTTGTGTGGATTCATGGCTGAAAAAATGGGGAAGAAACTGCCCAGTGTGCAAGCATGATATACGAAAGAAAATTGCGACTGCCGAGATCAAGAGAAAATCCTGGTTATACAATTAG

Protein Analysis

292

Amino Acids

32.31

Weight (kDa)

6.45

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 78 - 139 1.2e-13 PA domain
zf-rbx1 PF12678 220 - 271 1.5e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 228 - 271 2.4e-12 Ring finger domain
zf-C3HC4 PF00097 229 - 270 7.3e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 229 - 270 3.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 229 - 256 5.8e-09 RING-like zinc finger
zf-RING_UBOX PF13445 229 - 268 8.5e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AciI CCGC 2 cut(s) 111, 605
AcsI RAATTY 4 cut(s) 90, 116, 720, 749
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 3 cut(s) 209, 677, 759
AjnI CCWGG 2 cut(s) 479, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 3 cut(s) 11, 138, 592
AluI AGCT 3 cut(s) 11, 138, 592
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 530, 549
AoxI GGCC 2 cut(s) 477, 566
ApeKI GCWGC 1 cut(s) 450
ApoI RAATTY 4 cut(s) 90, 116, 720, 749
AspLEI GCGC 2 cut(s) 564, 689
AspS9I GGNCC 1 cut(s) 567
AsuHPI GGTGA 2 cut(s) 266, 419
AsuII TTCGAA 1 cut(s) 395
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 1 cut(s) 437
BccI CCATC 2 cut(s) 91, 698
BciT130I CCWGG 2 cut(s) 481, 862
BciVI GTATCC 1 cut(s) 601
BcoDI GTCTC 2 cut(s) 530, 549
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 565
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 1 cut(s) 451
BlsI GCNGC 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 481, 862
BmgT120I GGNCC 1 cut(s) 567
BmrFI CCNGG 2 cut(s) 481, 862
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 3 cut(s) 410, 462, 613
BmuI ACTGGG 1 cut(s) 797
BpiI GAAGAC 1 cut(s) 197
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 561, 719
Bsa29I ATCGAT 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 530
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 563, 803
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 2 cut(s) 481, 862
BseCI ATCGAT 1 cut(s) 500
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 2 cut(s) 563, 803
BseXI GCAGC 1 cut(s) 437
BshFI GGCC 2 cut(s) 479, 568
BshVI ATCGAT 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 530, 549
BsmI GAATGC 2 cut(s) 263, 452
BsnI GGCC 2 cut(s) 479, 568
Bso31I GGTCTC 1 cut(s) 530
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 2 cut(s) 501, 846
BspACI CCGC 2 cut(s) 111, 605
BspANI GGCC 2 cut(s) 479, 568
BspCNI CTCAG 1 cut(s) 562
BspDI ATCGAT 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 2 cut(s) 563, 803
BssMI GATC 2 cut(s) 501, 846
BssSI CACGAG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 2 cut(s) 481, 862
Bst4CI ACNGT 1 cut(s) 517
BstBI TTCGAA 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 56, 549
BstF5I GGATG 1 cut(s) 667
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 2 cut(s) 564, 689
BstKTI GATC 2 cut(s) 504, 849
BstMAI GTCTC 2 cut(s) 530, 549
BstMBI GATC 2 cut(s) 501, 846
BstMWI GCNNNNNNNGC 6 cut(s) 119, 293, 421, 545, 693, 807
BstNI CCWGG 2 cut(s) 481, 862
BstNSI RCATGY 2 cut(s) 182, 428
BstSCI CCNGG 2 cut(s) 479, 860
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 437
BstV2I GAAGAC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 500
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 2 cut(s) 479, 568
BsuTUI ATCGAT 1 cut(s) 500
BtsCI GGATG 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 810
CfoI GCGC 2 cut(s) 564, 689
Cfr13I GGNCC 1 cut(s) 567
ClaI ATCGAT 1 cut(s) 500
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 6 cut(s) 179, 349, 425, 746, 774, 815
CviJI RGCY 6 cut(s) 11, 138, 479, 568, 592, 778
CviKI_1 RGCY 6 cut(s) 11, 138, 479, 568, 592, 778
DdeI CTNAG 2 cut(s) 56, 549
DpnI GATC 2 cut(s) 503, 848
DpnII GATC 2 cut(s) 501, 846
Eco147I AGGCCT 1 cut(s) 479
Eco31I GGTCTC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 479, 860
FaeI CATG 6 cut(s) 182, 352, 428, 749, 777, 818
FatI CATG 6 cut(s) 178, 348, 424, 745, 773, 814
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 451
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 1 cut(s) 451
GlaI GCGC 2 cut(s) 563, 688
GluI GCNGC 1 cut(s) 451
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 2 cut(s) 479, 568
HapII CCGG 1 cut(s) 297
HhaI GCGC 2 cut(s) 564, 689
Hin1II CATG 6 cut(s) 182, 352, 428, 749, 777, 818
Hin6I GCGC 2 cut(s) 562, 687
HinP1I GCGC 2 cut(s) 562, 687
HinfI GANTC 4 cut(s) 21, 665, 713, 770
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 266, 419
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 4 cut(s) 142, 169, 552, 718
Hpy188III TCNNGA 2 cut(s) 87, 850
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 3 cut(s) 56, 116, 434
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4V TGCA 4 cut(s) 178, 280, 453, 810
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 293, 421, 545, 693, 807
HpyF3I CTNAG 2 cut(s) 56, 549
Hsp92II CATG 6 cut(s) 182, 352, 428, 749, 777, 818
HspAI GCGC 2 cut(s) 562, 687
Kzo9I GATC 2 cut(s) 501, 846
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 3 cut(s) 410, 462, 613
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 503, 848
MboI GATC 2 cut(s) 501, 846
MboII GAAGA 7 cut(s) 197, 448, 449, 594, 689, 725, 804
MhlI GDGCHC 1 cut(s) 550
MmeI TCCRAC 3 cut(s) 76, 103, 192
MnlI CCTC 4 cut(s) 201, 225, 245, 262
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 429
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 2 cut(s) 481, 862
Mva1269I GAATGC 2 cut(s) 263, 452
MvaI CCWGG 2 cut(s) 481, 862
MwoI GCNNNNNNNGC 6 cut(s) 119, 293, 421, 545, 693, 807
NdeII GATC 2 cut(s) 501, 846
NlaIII CATG 6 cut(s) 182, 352, 428, 749, 777, 818
NmeAIII GCCGAG 1 cut(s) 868
NspI RCATGY 2 cut(s) 182, 428
NspV TTCGAA 1 cut(s) 395
PaeI GCATGC 2 cut(s) 182, 428
PceI AGGCCT 1 cut(s) 479
PctI GAATGC 2 cut(s) 263, 452
PfeI GAWTC 4 cut(s) 21, 665, 713, 770
PkrI GCNGC 1 cut(s) 452
PsiI TTATAA 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 479, 860
PspGI CCWGG 2 cut(s) 479, 860
PspPI GGNCC 1 cut(s) 567
RseI CAYNNNNRTG 1 cut(s) 429
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 1 cut(s) 451
Sau3AI GATC 2 cut(s) 501, 846
Sau96I GGNCC 1 cut(s) 567
ScrFI CCNGG 2 cut(s) 481, 862
SduI GDGCHC 1 cut(s) 550
SetI ASST 6 cut(s) 13, 108, 140, 236, 256, 594
SfaNI GCATC 3 cut(s) 410, 462, 613
SfcI CTRYAG 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 576, 698
SmoI CTYRAG 2 cut(s) 576, 698
SphI GCATGC 2 cut(s) 182, 428
SseBI AGGCCT 1 cut(s) 479
SsiI CCGC 2 cut(s) 111, 605
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 479, 860
TaaI ACNGT 1 cut(s) 517
TaqI TCGA 3 cut(s) 267, 395, 500
TfiI GAWTC 4 cut(s) 21, 665, 713, 770
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 2 cut(s) 67, 810
TseI GCWGC 1 cut(s) 450
TspDTI ATGAA 5 cut(s) 486, 678, 743, 762, 762
TspRI CASTG 2 cut(s) 67, 810
XapI RAATTY 4 cut(s) 90, 116, 720, 749
XceI RCATGY 2 cut(s) 182, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.