pycom15g21890

serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004990
Physical Location & Seq
Forward (+)
15921673 .. 15924888
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1479 bp
ATGACGCGACTTCCGATGAAGACGTCATTTTCGTTCTCATTCTCCTTCTTCTTCACGATGATGCTCTGGGCGCCGCTCTCTTATGGCTTTGTGAATCCCCGAATACTTCTTCAGCTGGAGAGCTCATCGGGAAGTGGCGGCGGCAAGTACTTGACGAAGGAGGAGCTTTGGTTCGATCAGACAATCGACCACTTCTCGCCGTACGACCGCCGCAAATTCCCGCAGCGATACTACGAATTTCTCGACTACTTTAGACCTTCCGACGGACCCATTTTCCTCAAAATTTGCGGAGAATCGTCGTGCGGCGGCATCCCCAACGATTATCTGAGTGTGTTGGCTAAGAAGTTTGGGGCAGCTATCGTTTCTCCCGAGCATCGATACTATGGAAAGAGCTCTCCTTTCAAATCTCATACGACACAGAATTTGAAGTACTTGTCATCCAAGCAAGCGCTTTTCGATTTGGCGGCATTTCGTGAATTTTATCAGAATTGGTTAAATGTAAAGCTGAATAGAACCAAGGGTGACAATCCGTGGTTCGTGTTTGGTGTTTCTTACTCGGGAGCTCTTAGTGCGTGGTTTCGTGTTAAGTTTCCGCATTTGACATGTGGAAGTCTTGCAAGTTCTGCAGTTGTGGAAGCGATTTATGATTTCACTGAATTTGACCAGCAGATTGGTGAGTCTGCAGGTCCACAATGTAAAGCTGCACTTCAAGAAACTACTCGCATCGTTGAACAAAGTCTTACATCTAATGGAAAAGCAATAAGGGCGTTGTTTGGTGCAAGCCAGCTTGATATTGATGGTGACTTCATGTATTTTCTGGCGGATGCTGCTGTTATAGCGTTTCAGTATGGAAATCCCGATAAATTATGCTCACCTCTTGTTCAAGCAAAGACGAATGGAGAGGATTTGGTGGAAGCGTATGCCAAATACGTTAAAGATTTTTACCTTGGCACTTTTGGCGTTGACGTTGATACATATAACCAGAAACACTTGAAGAACACTGCTGTTAGTGAGGGCAGTTCTGATCGGTTGTGGTGGTTCCAAGTTTGTACTGAAGTTGCATATTTTCAGGTGGCCCCTGCAAATGATAGCATCAGGTCTTCAAAAGTTGACACAAGATACCATGAAGACCTTTGCAAAAATGTTTTTGGAGACAGCATCTATCCTGACGTTGTTGCAACAAACTTATACTATGGAGGCACAAAAATTGCAGGTTCAAAAATAGTTTTTACAAATGGCTCACAGGATCCCTGGCGTCATGCATCCAAACAAACTTCATCTCCAGATATGCCTTCGTACGTCATATCTTGCCATAACTGTGGCCATGGAACTGATTTGAGAGGATGCCCTCAGTCTCCTTTAACCCTTGAAGGCAATTCCCAGAACTGCAGCAACCCTGATGCAGTCAACAAAGTAAGGCAACAAATTGTTGAACACATTGACTTGTGGCTGTCGGAATGCCAAGAAACTGATGTTTGA

Protein Analysis

493

Amino Acids

55.28

Weight (kDa)

5.96

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 60 - 447 7.2e-74 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 674, 1202
AccB1I GGYRCC 1 cut(s) 70
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 7
AclWI GGATC 2 cut(s) 1241, 1254
AcoI YGGCCR 1 cut(s) 1321
AcsI RAATTY 6 cut(s) 215, 236, 282, 421, 476, 656
AcuI CTGAAG 2 cut(s) 95, 1074
AcyI GRCGYC 3 cut(s) 23, 71, 1255
AfaI GTAC 5 cut(s) 149, 203, 431, 1051, 1298
AfeI AGCGCT 1 cut(s) 450
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 602
AjnI CCWGG 1 cut(s) 1250
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 9 cut(s) 115, 123, 166, 356, 393, 505, 563, 701, 787
AluI AGCT 9 cut(s) 115, 123, 166, 356, 393, 505, 563, 701, 787
Alw21I GWGCWC 3 cut(s) 125, 395, 565
Alw26I GTCTC 2 cut(s) 1146, 1359
AlwI GGATC 2 cut(s) 1241, 1254
Ama87I CYCGRG 2 cut(s) 368, 556
Aor51HI AGCGCT 1 cut(s) 450
AoxI GGCC 2 cut(s) 1074, 1321
ApeKI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
ApoI RAATTY 6 cut(s) 215, 236, 282, 421, 476, 656
ArsI GACNNNNNNTTYG 4 cut(s) 13, 45, 406, 438
AspLEI GCGC 2 cut(s) 73, 451
AspS9I GGNCC 3 cut(s) 266, 686, 1075
AsuHPI GGTGA 4 cut(s) 533, 686, 812, 864
AvaI CYCGRG 2 cut(s) 368, 556
AvaII GGWCC 2 cut(s) 266, 686
BalI TGGCCA 1 cut(s) 1323
BamHI GGATCC 1 cut(s) 1246
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 3 cut(s) 125, 395, 565
BbsI GAAGAC 3 cut(s) 26, 1092, 1134
Bbv12I GWGCWC 3 cut(s) 125, 395, 565
BbvI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BccI CCATC 1 cut(s) 791
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 1252
BcoDI GTCTC 2 cut(s) 1146, 1359
BfmI CTRYAG 3 cut(s) 624, 681, 1387
BfoI RGCGCY 2 cut(s) 74, 452
BfuAI ACCTGC 2 cut(s) 674, 1202
BmcAI AGTACT 2 cut(s) 149, 431
Bme1390I CCNGG 1 cut(s) 1252
Bme18I GGWCC 2 cut(s) 266, 686
BmeT110I CYCGRG 2 cut(s) 368, 556
BmgT120I GGNCC 3 cut(s) 266, 686, 1075
BmiI GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
BmrFI CCNGG 1 cut(s) 1252
BpiI GAAGAC 3 cut(s) 26, 1092, 1134
BpmI CTGGAG 2 cut(s) 137, 1266
Bsa29I ATCGAT 1 cut(s) 376
BsaHI GRCGYC 3 cut(s) 23, 71, 1255
BsaJI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 1264, 1294
Bsc4I CCNNNNNNNGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 1252
BseCI ATCGAT 1 cut(s) 376
BseDI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BseGI GGATG 5 cut(s) 309, 437, 829, 1262, 1349
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMII CTCAG 2 cut(s) 317, 1364
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 2 cut(s) 1076, 1323
BshNI GGYRCC 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 376
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 3 cut(s) 125, 395, 565
BsiHKCI CYCGRG 2 cut(s) 368, 556
BsiWI CGTACG 2 cut(s) 201, 1296
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 1146, 1359
BsmI GAATGC 1 cut(s) 1463
BsnI GGCC 2 cut(s) 1076, 1323
BsoBI CYCGRG 2 cut(s) 368, 556
Bsp1286I GDGCHC 3 cut(s) 125, 395, 565
Bsp143I GATC 3 cut(s) 175, 1024, 1246
Bsp19I CCATGG 1 cut(s) 1324
BspANI GGCC 2 cut(s) 1076, 1323
BspCNI CTCAG 2 cut(s) 318, 1363
BspDI ATCGAT 1 cut(s) 376
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
BspMAI CTGCAG 3 cut(s) 628, 685, 1391
BspMI ACCTGC 2 cut(s) 674, 1202
BspPI GGATC 2 cut(s) 1241, 1254
BspT107I GGYRCC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 76
BssECI CCNNGG 5 cut(s) 516, 530, 946, 1250, 1324
BssMI GATC 3 cut(s) 175, 1024, 1246
BssNI GRCGYC 3 cut(s) 23, 71, 1255
BssT1I CCWWGG 3 cut(s) 516, 946, 1324
Bst2UI CCWGG 1 cut(s) 1252
Bst4CI ACNGT 1 cut(s) 1319
BstACI GRCGYC 3 cut(s) 23, 71, 1255
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 3 cut(s) 447, 781, 785
BstDEI CTNAG 4 cut(s) 326, 339, 566, 1350
BstDSI CCRYGG 2 cut(s) 530, 1324
BstF5I GGATG 5 cut(s) 309, 437, 829, 1262, 1349
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 2 cut(s) 74, 452
BstHHI GCGC 2 cut(s) 73, 451
BstKTI GATC 3 cut(s) 178, 1027, 1249
BstMAI GTCTC 2 cut(s) 1146, 1359
BstMBI GATC 3 cut(s) 175, 1024, 1246
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 7 cut(s) 70, 569, 623, 764, 827, 836, 957
BstNI CCWGG 1 cut(s) 1252
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 1 cut(s) 1250
BstSFI CTRYAG 3 cut(s) 624, 681, 1387
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
BstV2I GAAGAC 3 cut(s) 26, 1092, 1134
BstX2I RGATCY 1 cut(s) 1246
BstXI CCANNNNNNTGG 2 cut(s) 671, 1319
BstYI RGATCY 1 cut(s) 1246
Bsu15I ATCGAT 1 cut(s) 376
BsuRI GGCC 2 cut(s) 1076, 1323
BsuTUI ATCGAT 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 530, 1324
BtsCI GGATG 5 cut(s) 309, 437, 829, 1262, 1349
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 2 cut(s) 651, 999
BveI ACCTGC 2 cut(s) 674, 1202
Cac8I GCNNGC 3 cut(s) 447, 781, 785
CfoI GCGC 2 cut(s) 73, 451
Cfr13I GGNCC 3 cut(s) 266, 686, 1075
ClaI ATCGAT 1 cut(s) 376
CseI GACGC 2 cut(s) 13, 1244
Csp6I GTAC 5 cut(s) 148, 202, 430, 1050, 1297
CviAII CATG 5 cut(s) 603, 808, 1124, 1259, 1325
CviQI GTAC 5 cut(s) 148, 202, 430, 1050, 1297
DdeI CTNAG 4 cut(s) 326, 339, 566, 1350
DinI GGCGCC 1 cut(s) 72
DpnI GATC 3 cut(s) 177, 1026, 1248
DpnII GATC 3 cut(s) 175, 1024, 1246
EaeI YGGCCR 1 cut(s) 1321
EciI GGCGGA 1 cut(s) 836
Ecl136II GAGCTC 3 cut(s) 123, 393, 563
Eco130I CCWWGG 3 cut(s) 516, 946, 1324
Eco24I GRGCYC 3 cut(s) 125, 395, 565
Eco47I GGWCC 2 cut(s) 266, 686
Eco47III AGCGCT 1 cut(s) 450
Eco53kI GAGCTC 3 cut(s) 123, 393, 563
Eco57I CTGAAG 2 cut(s) 95, 1074
Eco88I CYCGRG 2 cut(s) 368, 556
EcoICRI GAGCTC 3 cut(s) 123, 393, 563
EcoRII CCWGG 1 cut(s) 1250
EcoT14I CCWWGG 3 cut(s) 516, 946, 1324
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 3 cut(s) 125, 395, 565
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 516, 946, 1324
FaeI CATG 5 cut(s) 606, 811, 1127, 1262, 1328
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FatI CATG 5 cut(s) 602, 807, 1123, 1258, 1324
FauI CCCGC 1 cut(s) 228
FokI GGATG 5 cut(s) 296, 424, 836, 1249, 1356
FriOI GRGCYC 3 cut(s) 125, 395, 565
GlaI GCGC 2 cut(s) 72, 450
GsuI CTGGAG 2 cut(s) 137, 1266
HaeII RGCGCY 2 cut(s) 74, 452
HaeIII GGCC 2 cut(s) 1076, 1323
HgaI GACGC 2 cut(s) 13, 1244
HhaI GCGC 2 cut(s) 73, 451
Hin1I GRCGYC 3 cut(s) 23, 71, 1255
Hin1II CATG 5 cut(s) 606, 811, 1127, 1262, 1328
Hin6I GCGC 2 cut(s) 71, 449
HinP1I GCGC 2 cut(s) 71, 449
HincII GTYRAC 3 cut(s) 964, 1111, 1408
HindII GTYRAC 3 cut(s) 964, 1111, 1408
HinfI GANTC 3 cut(s) 94, 293, 677
HphI GGTGA 4 cut(s) 533, 686, 812, 864
Hpy166II GTNNAC 4 cut(s) 689, 964, 1111, 1408
Hpy188I TCNGA 7 cut(s) 15, 180, 262, 327, 486, 1024, 1456
Hpy8I GTNNAC 4 cut(s) 689, 964, 1111, 1408
Hpy99I CGWCG 2 cut(s) 266, 301
HpyAV CCTTC 5 cut(s) 55, 151, 267, 1302, 1364
HpyCH4III ACNGT 1 cut(s) 1319
HpyCH4IV ACGT 5 cut(s) 23, 930, 966, 1170, 1299
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 569, 623, 764, 827, 836, 957
HpyF3I CTNAG 4 cut(s) 326, 339, 566, 1350
HpySE526I ACGT 5 cut(s) 23, 930, 966, 1170, 1299
Hsp92I GRCGYC 3 cut(s) 23, 71, 1255
Hsp92II CATG 5 cut(s) 606, 811, 1127, 1262, 1328
HspAI GCGC 2 cut(s) 71, 449
KasI GGCGCC 1 cut(s) 70
Kzo9I GATC 3 cut(s) 175, 1024, 1246
LmnI GCTCC 2 cut(s) 163, 560
Lsp1109I GCAGC 5 cut(s) 235, 365, 688, 814, 1401
MaeII ACGT 5 cut(s) 23, 930, 966, 1170, 1299
MaeIII GTNAC 2 cut(s) 521, 800
MalI GATC 3 cut(s) 177, 1026, 1248
MbiI CCGCTC 1 cut(s) 76
MboI GATC 3 cut(s) 175, 1024, 1246
MboII GAAGA 7 cut(s) 31, 40, 43, 101, 1006, 1092, 1139
MflI RGATCY 1 cut(s) 1246
MhlI GDGCHC 3 cut(s) 125, 395, 565
MlsI TGGCCA 1 cut(s) 1323
MluNI TGGCCA 1 cut(s) 1323
Mly113I GGCGCC 1 cut(s) 71
MlyI GAGTC 1 cut(s) 686
MmeI TCCRAC 2 cut(s) 285, 1434
MnlI CCTC 8 cut(s) 154, 287, 885, 895, 1006, 1190, 1334, 1359
Mox20I TGGCCA 1 cut(s) 1323
Mph1103I ATGCAT 1 cut(s) 1264
MscI TGGCCA 1 cut(s) 1323
MseI TTAA 4 cut(s) 494, 585, 933, 1361
MslI CAYNNNNRTG 2 cut(s) 59, 1317
Msp20I TGGCCA 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 1252
Mva1269I GAATGC 1 cut(s) 1463
MvaI CCWGG 1 cut(s) 1252
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 7 cut(s) 70, 569, 623, 764, 827, 836, 957
NarI GGCGCC 1 cut(s) 71
NcoI CCATGG 1 cut(s) 1324
NdeII GATC 3 cut(s) 175, 1024, 1246
NlaIII CATG 5 cut(s) 606, 811, 1127, 1262, 1328
NlaIV GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
NmuCI GTSAC 2 cut(s) 521, 800
NsiI ATGCAT 1 cut(s) 1264
NspI RCATGY 1 cut(s) 606
PciI ACATGT 1 cut(s) 602
PcsI WCGNNNNNNNCGW 4 cut(s) 11, 29, 240, 366
PctI GAATGC 1 cut(s) 1463
PfeI GAWTC 2 cut(s) 94, 293
Pfl23II CGTACG 2 cut(s) 201, 1296
PleI GAGTC 1 cut(s) 685
PluTI GGCGCC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 685
PscI ACATGT 1 cut(s) 602
Psp124BI GAGCTC 3 cut(s) 125, 395, 565
Psp6I CCWGG 1 cut(s) 1250
PspGI CCWGG 1 cut(s) 1250
PspLI CGTACG 2 cut(s) 201, 1296
PspN4I GGNNCC 5 cut(s) 72, 268, 1040, 1077, 1248
PspPI GGNCC 3 cut(s) 266, 686, 1075
PstI CTGCAG 3 cut(s) 628, 685, 1391
PsuI RGATCY 1 cut(s) 1246
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 5 cut(s) 149, 203, 431, 1051, 1298
RsaNI GTAC 5 cut(s) 148, 202, 430, 1050, 1297
RseI CAYNNNNRTG 2 cut(s) 59, 1317
SacI GAGCTC 3 cut(s) 125, 395, 565
SaqAI TTAA 4 cut(s) 494, 585, 933, 1361
Sau3AI GATC 3 cut(s) 175, 1024, 1246
Sau96I GGNCC 3 cut(s) 266, 686, 1075
ScaI AGTACT 2 cut(s) 149, 431
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 1 cut(s) 1252
SduI GDGCHC 3 cut(s) 125, 395, 565
SfcI CTRYAG 3 cut(s) 624, 681, 1387
SfoI GGCGCC 1 cut(s) 72
SinI GGWCC 2 cut(s) 266, 686
SmiMI CAYNNNNRTG 2 cut(s) 59, 1317
SspDI GGCGCC 1 cut(s) 70
SstI GAGCTC 3 cut(s) 125, 395, 565
StyD4I CCNGG 1 cut(s) 1250
StyI CCWWGG 3 cut(s) 516, 946, 1324
TaaI ACNGT 1 cut(s) 1319
TaiI ACGT 5 cut(s) 26, 933, 969, 1173, 1302
TaqI TCGA 5 cut(s) 174, 186, 243, 376, 456
TatI WGTACW 3 cut(s) 147, 429, 1049
TauI GCSGC 7 cut(s) 76, 141, 144, 213, 306, 309, 467
TfiI GAWTC 2 cut(s) 94, 293
Tru1I TTAA 4 cut(s) 494, 585, 933, 1361
Tru9I TTAA 4 cut(s) 494, 585, 933, 1361
TscAI CASTG 2 cut(s) 658, 1006
TseFI GTSAC 2 cut(s) 521, 800
TseI GCWGC 5 cut(s) 223, 353, 701, 827, 1389
Tsp45I GTSAC 2 cut(s) 521, 800
TspDTI ATGAA 4 cut(s) 32, 796, 1140, 1266
TspGWI ACGGA 2 cut(s) 279, 519
TspRI CASTG 2 cut(s) 658, 1006
VpaK11BI GGWCC 2 cut(s) 266, 686
XapI RAATTY 6 cut(s) 215, 236, 282, 421, 476, 656
XceI RCATGY 1 cut(s) 606
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 2 cut(s) 149, 431
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.