pycom15g22850

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008672
Physical Location & Seq
Forward (+)
16919580 .. 16921362
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGAAAGAGATAAATGAGAATTTTATGTCAAGTAGAAAAACTAATGAAAATGTGCAAAGTGTAAAGCCAGCAATGTTCTTGGACAAGGCATCTGAGGCCTGGACCAGATTCTCACTAAACTCAATGGAGTTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTGATTTCGGAGGATCATAATGATAATTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTACGATGCGGTTATTCTTGTCCACAGAGAAAAATTACCTTACCCCCAACATCTTCTCTTCCCATCCGAATTAAATCAAGGCGCAGGGGTACATGTGGCACGTGGGAATGCGAGCAAGGTTTTCCACCCATTTCTGTTGCCTGGAGACATGAAGGGAAACTCGGAGGCACTACGAAATAAGCGGTTGGTAGACTTTGATCCACTGAGGTGCTTTGTTGGGGATCTAGAGAAAGAGTACCCCAACACCTTCAAGCTGTGGTATGATTCTCTGGGTGGTGATGCTGTTGGCATAACGTGGGGGAGATATGGTTCAAAGAAGCGAGGGCGGGAAGGAGAAGCAGAGGAAGTAAAAGATCCAACTGATTTATTAAACGACACCGGTAAAGTGGGAACAGGGTTTGTGAGGGGGGTTTATCTTCCCAAAGCCCCAAGGCTCACGAGTTGGCTCTGTTTATACTCTAAAATGCTCAAAGCTTCACGGGCGACAACACTTTTCTCCGTGAGCTACTGCTGCTTTTCGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

257

Amino Acids

29.04

Weight (kDa)

8.98

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap_D6 PF17407 80 - 191 7.7e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 435
AciI CCGC 3 cut(s) 254, 427, 571
AclWI GGATC 4 cut(s) 201, 437, 474, 593
AcsI RAATTY 1 cut(s) 19
AcvI CACGTG 1 cut(s) 347
AfaI GTAC 2 cut(s) 336, 482
AflIII ACRYGT 1 cut(s) 337
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 3 cut(s) 241, 496, 558
AjnI CCWGG 2 cut(s) 98, 385
AleI CACNNNNGTG 1 cut(s) 451
AloI GAACNNNNNNTCC 2 cut(s) 538, 570
AluBI AGCT 3 cut(s) 499, 719, 750
AluI AGCT 3 cut(s) 499, 719, 750
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 4 cut(s) 201, 437, 474, 593
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 756
ApoI RAATTY 1 cut(s) 19
AsiGI ACCGGT 1 cut(s) 623
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 533
AsuII TTCGAA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 102
BauI CACGAG 1 cut(s) 682
BbrPI CACGTG 1 cut(s) 347
BbvI GCAGC 1 cut(s) 743
BccI CCATC 3 cut(s) 136, 207, 316
BciT130I CCWGG 2 cut(s) 100, 387
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 1 cut(s) 470
BisI GCNGC 1 cut(s) 757
BlsI GCNGC 1 cut(s) 758
Bme1390I CCNGG 2 cut(s) 100, 387
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 100, 387
BmsI GCATC 3 cut(s) 98, 241, 514
BpmI CTGGAG 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 674
BsaWI WCCGGW 1 cut(s) 623
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bse118I RCCGGY 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 100, 387
BseDI CCNNGG 1 cut(s) 674
BseGI GGATG 1 cut(s) 308
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 2 cut(s) 84, 440
BseXI GCAGC 1 cut(s) 743
BshFI GGCC 1 cut(s) 98
BshTI ACCGGT 1 cut(s) 623
BsiSI CCGG 1 cut(s) 624
BsmAI GTCTC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 229
Bsp143I GATC 4 cut(s) 193, 442, 466, 598
BspACI CCGC 3 cut(s) 254, 427, 571
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 85, 441
BspPI GGATC 4 cut(s) 201, 437, 474, 593
BspT104I TTCGAA 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 623
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 1 cut(s) 674
BssMI GATC 4 cut(s) 193, 442, 466, 598
BssSI CACGAG 1 cut(s) 682
BssT1I CCWWGG 1 cut(s) 674
Bst2BI CACGAG 1 cut(s) 682
Bst2UI CCWGG 2 cut(s) 100, 387
Bst6I CTCTTC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 69, 358
BstDEI CTNAG 2 cut(s) 93, 449
BstF5I GGATG 1 cut(s) 308
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 196, 445, 469, 601
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 4 cut(s) 193, 442, 466, 598
BstMWI GCNNNNNNNGC 3 cut(s) 95, 725, 756
BstNI CCWGG 2 cut(s) 100, 387
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 98, 385
BstV1I GCAGC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 466, 598
BstYI RGATCY 2 cut(s) 466, 598
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 166, 446
Cac8I GCNNGC 2 cut(s) 69, 358
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 102
Csp6I GTAC 2 cut(s) 335, 481
CspAI ACCGGT 1 cut(s) 623
CviAII CATG 3 cut(s) 338, 394, 768
CviJI RGCY 9 cut(s) 67, 98, 146, 499, 671, 679, 691, 719, 750
CviKI_1 RGCY 9 cut(s) 67, 98, 146, 499, 671, 679, 691, 719, 750
CviQI GTAC 2 cut(s) 335, 481
DdeI CTNAG 2 cut(s) 93, 449
DpnI GATC 4 cut(s) 195, 444, 468, 600
DpnII GATC 4 cut(s) 193, 442, 466, 598
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 674
Eco147I AGGCCT 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 98, 385
EcoT14I CCWWGG 1 cut(s) 674
ErhI CCWWGG 1 cut(s) 674
FaeI CATG 3 cut(s) 341, 397, 771
FatI CATG 3 cut(s) 337, 393, 767
FauI CCCGC 1 cut(s) 564
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 1 cut(s) 757
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 757
FspBI CTAG 1 cut(s) 470
GlaI GCGC 1 cut(s) 328
GluI GCNGC 1 cut(s) 757
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 624
HhaI GCGC 1 cut(s) 329
Hin1II CATG 3 cut(s) 341, 397, 771
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 2 cut(s) 108, 509
HpaII CCGG 1 cut(s) 624
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 3 cut(s) 174, 268, 436
Hpy188I TCNGA 4 cut(s) 94, 190, 313, 409
Hpy188III TCNNGA 2 cut(s) 470, 682
Hpy8I GTNNAC 3 cut(s) 174, 268, 436
HpyAV CCTTC 3 cut(s) 391, 502, 569
HpyCH4IV ACGT 2 cut(s) 346, 539
HpyCH4V TGCA 2 cut(s) 55, 164
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 725, 756
HpyF3I CTNAG 2 cut(s) 93, 449
HpySE526I ACGT 2 cut(s) 346, 539
Hsp92II CATG 3 cut(s) 341, 397, 771
HspAI GCGC 1 cut(s) 327
Kzo9I GATC 4 cut(s) 193, 442, 466, 598
Lsp1109I GCAGC 1 cut(s) 743
LweI GCATC 3 cut(s) 98, 241, 514
MaeI CTAG 1 cut(s) 470
MaeII ACGT 2 cut(s) 346, 539
MalI GATC 4 cut(s) 195, 444, 468, 600
MboI GATC 4 cut(s) 193, 442, 466, 598
MboII GAAGA 3 cut(s) 290, 295, 653
MflI RGATCY 2 cut(s) 466, 598
MluCI AATT 5 cut(s) 19, 205, 244, 278, 314
MmeI TCCRAC 1 cut(s) 626
MnlI CCTC 7 cut(s) 88, 184, 403, 444, 560, 580, 642
MseI TTAA 3 cut(s) 131, 317, 614
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 1 cut(s) 624
MspR9I CCNGG 2 cut(s) 100, 387
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 2 cut(s) 100, 387
MwoI GCNNNNNNNGC 3 cut(s) 95, 725, 756
NdeII GATC 4 cut(s) 193, 442, 466, 598
NlaIII CATG 3 cut(s) 341, 397, 771
NspI RCATGY 1 cut(s) 341
NspV TTCGAA 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 451
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 337
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 2 cut(s) 108, 509
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 1 cut(s) 758
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 347
PscI ACATGT 1 cut(s) 337
Psp6I CCWGG 2 cut(s) 98, 385
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 2 cut(s) 98, 385
PspPI GGNCC 1 cut(s) 102
PsuI RGATCY 2 cut(s) 466, 598
RsaI GTAC 2 cut(s) 336, 482
RsaNI GTAC 2 cut(s) 335, 481
RseI CAYNNNNRTG 1 cut(s) 451
SaqAI TTAA 3 cut(s) 131, 317, 614
SatI GCNGC 1 cut(s) 757
Sau3AI GATC 4 cut(s) 193, 442, 466, 598
Sau96I GGNCC 1 cut(s) 102
ScrFI CCNGG 2 cut(s) 100, 387
SfaNI GCATC 3 cut(s) 98, 241, 514
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 451
Sse9I AATT 5 cut(s) 19, 205, 244, 278, 314
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 3 cut(s) 254, 427, 571
SspMI CTAG 1 cut(s) 470
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 98, 385
StyI CCWWGG 1 cut(s) 674
TaiI ACGT 2 cut(s) 349, 542
TaqI TCGA 2 cut(s) 229, 764
TasI AATT 5 cut(s) 19, 205, 244, 278, 314
TfiI GAWTC 2 cut(s) 108, 509
Tru1I TTAA 3 cut(s) 131, 317, 614
Tru9I TTAA 3 cut(s) 131, 317, 614
TscAI CASTG 2 cut(s) 166, 453
TseI GCWGC 1 cut(s) 756
TspDTI ATGAA 3 cut(s) 17, 60, 410
TspGWI ACGGA 1 cut(s) 733
TspRI CASTG 2 cut(s) 166, 453
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 1 cut(s) 341
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.