pycom15g23380

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011209
Physical Location & Seq
Forward (+)
17428058 .. 17430487
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGCGTTCAAGAAACCCCTAGCCGAGCGCCTGTTCAACATCTCTAAGGCGTCGACCCAAACCCTCAGAAACTGCCGGATTTCGTCGTCCGCGGTCCACACCCGACCCGCTCCCAAGCCCCGCGAGACTAGAATCGCCCCCGACCCTGGGGACAATGCCATTTTCCGGAGGTTCCTCCACAAAAGATCGTCGGCGGGGAATTTCCAGAAGCAGGAGATCTGGTCTTTGCCGATGGGGGCAAACCTGATGGAGAAGCTCAAAGTCGTGGCCATTGGCCGGGACCGGATCCGACTGGACGGTTTGGCCCCGCCGGAACCCGAGTCGAAGAGCTCGCCGATGGAGAAATCGGGATTGACGACGGAGGAAACTAAGAAACTGCTGAGGGTGGGCCAGCTGGAAGCCGTGAAATCGAAGCTTCGAGAAGTCCAGAAAGGCTGGGTATCGTATCCGGAGTTTGTTGGGATCTGCCGGGAAGGTTGCGCGGATCCGGATATGGGTCTTGGGTTTGCGAAATCGTTGGACGAAAACGGTTCCGTGATCGTATTGGGAAATTCCGTTTGCTTGAGGCCTGAACAGATAGCCAAAGCAATCCAGGAACTAATTCCTATTCCAGGAACAACAACCAACTCCAATGATCCCAGGATTAGGGAATTGGAAGACATGGAGAGTCACAAGTTTGAGATAGACCGAAAGGCGGAGTCCCTTGTTCGAAAGGAGCTTTTGTGCGGTCTTGGCTATTTGGTTGTCTCCACAGCCGGATTCATGAGGCTCACATTCTGGGAACTCTCATGGGACGTCATGGAACCCATTTGCTTCTTCGTTACTTCAATGTACTTCATAGCCGGATACGCCTTCTTTCTCAGGACCCGGAAAGAGCCCTCTTTCGAAGGGTTTCGCCAGATTCGATTTGAGGCTAAGCAGAAGCGCCTCATGAAGCTTGAGAACTTCGATGTCGGAAGGTATGACGAGCTTCGTAAAGCATGTTGTCCCTACTCGTCGGCTTCATCAGAAATGACCCTGTCAACTGCTTTTGATGGGCCAAAGAGGATGCAATTTGGTTCAGTACATAGTTAA

Protein Analysis

358

Amino Acids

40.37

Weight (kDa)

9.25

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 164 - 324 8.8e-50 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 798
AccBSI CCGCTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 53
AccII CGCG 3 cut(s) 92, 123, 482
AccIII TCCGGA 3 cut(s) 165, 448, 487
AciI CCGC 9 cut(s) 90, 92, 108, 121, 194, 308, 482, 695, 726
AclWI GGATC 6 cut(s) 280, 293, 470, 479, 492, 629
AcoI YGGCCR 2 cut(s) 267, 274
AcsI RAATTY 2 cut(s) 199, 550
AcyI GRCGYC 2 cut(s) 50, 795
AfaI GTAC 2 cut(s) 833, 1065
AfiI CCNNNNNNNGG 9 cut(s) 146, 147, 165, 211, 276, 494, 611, 645, 694
AgsI TTSAA 3 cut(s) 10, 37, 828
AjnI CCWGG 4 cut(s) 145, 591, 610, 638
AluBI AGCT 7 cut(s) 256, 330, 394, 415, 718, 937, 970
AluI AGCT 7 cut(s) 256, 330, 394, 415, 718, 937, 970
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 2 cut(s) 119, 751
AlwI GGATC 6 cut(s) 280, 293, 470, 479, 492, 629
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 3 cut(s) 165, 448, 487
AoxI GGCC 6 cut(s) 267, 274, 303, 388, 566, 1037
ApoI RAATTY 2 cut(s) 199, 550
Asp700I GAANNNNTTC 2 cut(s) 600, 891
AspLEI GCGC 3 cut(s) 30, 482, 927
AspS9I GGNCC 6 cut(s) 94, 280, 304, 388, 864, 1037
AsuC2I CCSGG 3 cut(s) 278, 470, 868
AsuII TTCGAA 2 cut(s) 709, 885
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 3 cut(s) 94, 280, 864
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 2 cut(s) 285, 484
BanII GRGCYC 2 cut(s) 332, 879
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 380
BccI CCATC 4 cut(s) 226, 241, 331, 1028
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 4 cut(s) 147, 593, 612, 640
BciVI GTATCC 2 cut(s) 456, 839
BcnI CCSGG 3 cut(s) 278, 470, 868
BcoDI GTCTC 2 cut(s) 119, 751
BfaI CTAG 2 cut(s) 20, 129
BfoI RGCGCY 2 cut(s) 31, 928
BfuI GTATCC 2 cut(s) 456, 839
BglII AGATCT 1 cut(s) 216
BlpI GCTNAGC 1 cut(s) 915
Bme1390I CCNGG 7 cut(s) 147, 278, 470, 593, 612, 640, 868
Bme18I GGWCC 3 cut(s) 94, 280, 864
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 6 cut(s) 94, 280, 304, 388, 864, 1037
BmiI GGNNCC 9 cut(s) 173, 281, 287, 306, 315, 486, 532, 804, 866
BmrFI CCNGG 7 cut(s) 147, 278, 470, 593, 612, 640, 868
BmsI GCATC 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 663
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 2 cut(s) 709, 885
BpuEI CTTGAG 2 cut(s) 583, 959
BpuMI CCSGG 3 cut(s) 278, 470, 868
BsaHI GRCGYC 2 cut(s) 50, 795
BsaJI CCNNGG 4 cut(s) 90, 145, 146, 638
BsaWI WCCGGW 4 cut(s) 165, 282, 448, 487
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 707, 737
Bsc4I CCNNNNNNNGG 9 cut(s) 146, 147, 165, 211, 276, 494, 611, 645, 694
Bse1I ACTGG 1 cut(s) 297
BseAI TCCGGA 3 cut(s) 165, 448, 487
BseBI CCWGG 4 cut(s) 147, 593, 612, 640
BseDI CCNNGG 4 cut(s) 90, 145, 146, 638
BseGI GGATG 1 cut(s) 1053
BseLI CCNNNNNNNGG 9 cut(s) 146, 147, 165, 211, 276, 494, 611, 645, 694
BseMII CTCAG 3 cut(s) 79, 371, 874
BseNI ACTGG 1 cut(s) 297
BseYI CCCAGC 1 cut(s) 435
Bsh1236I CGCG 3 cut(s) 92, 123, 482
BshFI GGCC 6 cut(s) 269, 276, 305, 390, 568, 1039
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 317
BslFI GGGAC 5 cut(s) 164, 293, 685, 806, 972
BslI CCNNNNNNNGG 9 cut(s) 146, 147, 165, 211, 276, 494, 611, 645, 694
BsmAI GTCTC 2 cut(s) 119, 751
BsmFI GGGAC 5 cut(s) 164, 293, 685, 806, 972
BsnI GGCC 6 cut(s) 269, 276, 305, 390, 568, 1039
BsoBI CYCGRG 1 cut(s) 317
Bsp119I TTCGAA 2 cut(s) 709, 885
Bsp1286I GDGCHC 2 cut(s) 332, 879
Bsp13I TCCGGA 3 cut(s) 165, 448, 487
Bsp143I GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 9 cut(s) 90, 92, 108, 121, 194, 308, 482, 695, 726
BspANI GGCC 6 cut(s) 269, 276, 305, 390, 568, 1039
BspCNI CTCAG 3 cut(s) 78, 372, 873
BspEI TCCGGA 3 cut(s) 165, 448, 487
BspFNI CGCG 3 cut(s) 92, 123, 482
BspHI TCATGA 2 cut(s) 762, 930
BspLI GGNNCC 9 cut(s) 173, 281, 287, 306, 315, 486, 532, 804, 866
BspPI GGATC 6 cut(s) 280, 293, 470, 479, 492, 629
BspQI GCTCTTC 1 cut(s) 320
BspT104I TTCGAA 2 cut(s) 709, 885
BsrBI CCGCTC 1 cut(s) 110
BsrI ACTGG 1 cut(s) 297
BssECI CCNNGG 4 cut(s) 90, 145, 146, 638
BssMI GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
BssNI GRCGYC 2 cut(s) 50, 795
Bst2UI CCWGG 4 cut(s) 147, 593, 612, 640
Bst4CI ACNGT 2 cut(s) 299, 530
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 2 cut(s) 50, 795
BstBI TTCGAA 2 cut(s) 709, 885
BstC8I GCNNGC 2 cut(s) 332, 392
BstDEI CTNAG 6 cut(s) 45, 65, 369, 380, 860, 915
BstDSI CCRYGG 1 cut(s) 90
BstENI CCTNNNNNAGG 1 cut(s) 609
BstF5I GGATG 1 cut(s) 1053
BstFNI CGCG 3 cut(s) 92, 123, 482
BstH2I RGCGCY 2 cut(s) 31, 928
BstHHI GCGC 3 cut(s) 30, 482, 927
BstKTI GATC 7 cut(s) 188, 219, 288, 465, 487, 540, 637
BstMAI GTCTC 2 cut(s) 119, 751
BstMBI GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
BstMWI GCNNNNNNNGC 2 cut(s) 732, 848
BstNI CCWGG 4 cut(s) 147, 593, 612, 640
BstNSI RCATGY 1 cut(s) 984
BstSCI CCNGG 7 cut(s) 145, 276, 468, 591, 610, 638, 866
BstUI CGCG 3 cut(s) 92, 123, 482
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 4 cut(s) 216, 285, 462, 484
BstYI RGATCY 4 cut(s) 216, 285, 462, 484
BsuI GTATCC 2 cut(s) 456, 839
BsuRI GGCC 6 cut(s) 269, 276, 305, 390, 568, 1039
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 1053
Cac8I GCNNGC 2 cut(s) 332, 392
CaiI CAGNNNCTG 1 cut(s) 72
CciI TCATGA 2 cut(s) 762, 930
CfoI GCGC 3 cut(s) 30, 482, 927
Cfr13I GGNCC 6 cut(s) 94, 280, 304, 388, 864, 1037
Cfr42I CCGCGG 1 cut(s) 93
CseI GACGC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 832, 1064
CviAII CATG 6 cut(s) 661, 763, 789, 799, 931, 981
CviQI GTAC 2 cut(s) 832, 1064
DdeI CTNAG 6 cut(s) 45, 65, 369, 380, 860, 915
DpnI GATC 7 cut(s) 187, 218, 287, 464, 486, 539, 636
DpnII GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
EaeI YGGCCR 2 cut(s) 267, 274
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
EciI GGCGGA 1 cut(s) 710
Ecl136II GAGCTC 1 cut(s) 330
Eco147I AGGCCT 1 cut(s) 568
Eco24I GRGCYC 2 cut(s) 332, 879
Eco47I GGWCC 3 cut(s) 94, 280, 864
Eco53kI GAGCTC 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 330
EcoNI CCTNNNNNAGG 1 cut(s) 609
EcoO109I RGGNCCY 1 cut(s) 864
EcoRII CCWGG 4 cut(s) 145, 591, 610, 638
EcoT38I GRGCYC 2 cut(s) 332, 879
FaeI CATG 6 cut(s) 664, 766, 792, 802, 934, 984
FaqI GGGAC 5 cut(s) 164, 293, 685, 806, 972
FatI CATG 6 cut(s) 660, 762, 788, 798, 930, 980
FauI CCCGC 4 cut(s) 115, 128, 187, 315
FblI GTMKAC 1 cut(s) 53
FokI GGATG 1 cut(s) 1060
FriOI GRGCYC 2 cut(s) 332, 879
FspBI CTAG 2 cut(s) 20, 129
GlaI GCGC 3 cut(s) 29, 481, 926
GsaI CCCAGC 1 cut(s) 439
HaeII RGCGCY 2 cut(s) 31, 928
HaeIII GGCC 6 cut(s) 269, 276, 305, 390, 568, 1039
HgaI GACGC 1 cut(s) 39
HhaI GCGC 3 cut(s) 30, 482, 927
Hin1I GRCGYC 2 cut(s) 50, 795
Hin1II CATG 6 cut(s) 664, 766, 792, 802, 934, 984
Hin6I GCGC 3 cut(s) 28, 480, 925
HinP1I GCGC 3 cut(s) 28, 480, 925
HincII GTYRAC 2 cut(s) 54, 1023
HindII GTYRAC 2 cut(s) 54, 1023
HindIII AAGCTT 2 cut(s) 413, 935
HinfI GANTC 6 cut(s) 132, 320, 667, 698, 759, 901
Hpy166II GTNNAC 3 cut(s) 54, 97, 1023
Hpy188I TCNGA 4 cut(s) 68, 290, 956, 1009
Hpy8I GTNNAC 3 cut(s) 54, 97, 1023
Hpy99I CGWCG 5 cut(s) 55, 88, 193, 361, 1000
HpyAV CCTTC 4 cut(s) 467, 862, 881, 951
HpyCH4III ACNGT 2 cut(s) 299, 530
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 1 cut(s) 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 732, 848
HpyF3I CTNAG 6 cut(s) 45, 65, 369, 380, 860, 915
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 2 cut(s) 50, 795
Hsp92II CATG 6 cut(s) 664, 766, 792, 802, 934, 984
HspAI GCGC 3 cut(s) 28, 480, 925
Kpn2I TCCGGA 3 cut(s) 165, 448, 487
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 2 cut(s) 115, 715
LweI GCATC 1 cut(s) 1038
MaeI CTAG 2 cut(s) 20, 129
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 2 cut(s) 668, 820
MalI GATC 7 cut(s) 187, 218, 287, 464, 486, 539, 636
MbiI CCGCTC 1 cut(s) 110
MboI GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
MboII GAAGA 3 cut(s) 337, 668, 808
MflI RGATCY 4 cut(s) 216, 285, 462, 484
MhlI GDGCHC 2 cut(s) 332, 879
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 5 cut(s) 199, 550, 600, 650, 1052
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 3 cut(s) 329, 676, 707
MmeI TCCRAC 3 cut(s) 313, 498, 934
Mox20I TGGCCA 1 cut(s) 269
MroI TCCGGA 3 cut(s) 165, 448, 487
MroXI GAANNNNTTC 2 cut(s) 600, 891
MscI TGGCCA 1 cut(s) 269
MseI TTAA 1 cut(s) 1072
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 2 cut(s) 92, 394
MspR9I CCNGG 7 cut(s) 147, 278, 470, 593, 612, 640, 868
MvaI CCWGG 4 cut(s) 147, 593, 612, 640
MvnI CGCG 3 cut(s) 92, 123, 482
MwoI GCNNNNNNNGC 2 cut(s) 732, 848
NciI CCSGG 3 cut(s) 278, 470, 868
NdeII GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
NlaIII CATG 6 cut(s) 664, 766, 792, 802, 934, 984
NlaIV GGNNCC 9 cut(s) 173, 281, 287, 306, 315, 486, 532, 804, 866
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 668
NspI RCATGY 1 cut(s) 984
NspV TTCGAA 2 cut(s) 709, 885
PagI TCATGA 2 cut(s) 762, 930
PasI CCCWGGG 1 cut(s) 146
PceI AGGCCT 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 2 cut(s) 353, 901
PdmI GAANNNNTTC 2 cut(s) 600, 891
PfeI GAWTC 3 cut(s) 132, 759, 901
PflFI GACNNNGTC 1 cut(s) 1018
PfoI TCCNGGA 2 cut(s) 591, 610
PleI GAGTC 3 cut(s) 328, 675, 706
PpsI GAGTC 3 cut(s) 328, 675, 706
PpuMI RGGWCCY 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 864
Psp6I CCWGG 4 cut(s) 145, 591, 610, 638
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 4 cut(s) 145, 591, 610, 638
PspN4I GGNNCC 9 cut(s) 173, 281, 287, 306, 315, 486, 532, 804, 866
PspPI GGNCC 6 cut(s) 94, 280, 304, 388, 864, 1037
PspPPI RGGWCCY 1 cut(s) 864
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 4 cut(s) 216, 285, 462, 484
PsyI GACNNNGTC 1 cut(s) 1018
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 2 cut(s) 833, 1065
RsaNI GTAC 2 cut(s) 832, 1064
SacI GAGCTC 1 cut(s) 332
SacII CCGCGG 1 cut(s) 93
SalI GTCGAC 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 1072
Sau3AI GATC 7 cut(s) 185, 216, 285, 462, 484, 537, 634
Sau96I GGNCC 6 cut(s) 94, 280, 304, 388, 864, 1037
SchI GAGTC 3 cut(s) 329, 676, 707
ScrFI CCNGG 7 cut(s) 147, 278, 470, 593, 612, 640, 868
SduI GDGCHC 2 cut(s) 332, 879
SfaNI GCATC 1 cut(s) 1038
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 2 cut(s) 709, 885
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 3 cut(s) 94, 280, 864
SmlI CTYRAG 2 cut(s) 562, 938
SmoI CTYRAG 2 cut(s) 562, 938
Sse9I AATT 5 cut(s) 199, 550, 600, 650, 1052
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 9 cut(s) 90, 92, 108, 121, 194, 308, 482, 695, 726
SspMI CTAG 2 cut(s) 20, 129
SstI GAGCTC 1 cut(s) 332
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 7 cut(s) 145, 276, 468, 591, 610, 638, 866
TaaI ACNGT 2 cut(s) 299, 530
TaiI ACGT 1 cut(s) 798
TaqI TCGA 8 cut(s) 53, 323, 410, 418, 709, 885, 904, 948
TaqII GACCGA 1 cut(s) 702
TasI AATT 5 cut(s) 199, 550, 600, 650, 1052
TatI WGTACW 2 cut(s) 831, 1063
TfiI GAWTC 3 cut(s) 132, 759, 901
Tru1I TTAA 1 cut(s) 1072
Tru9I TTAA 1 cut(s) 1072
TseFI GTSAC 1 cut(s) 668
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 4 cut(s) 751, 826, 947, 993
TspGWI ACGGA 3 cut(s) 374, 523, 544
Tth111I GACNNNGTC 1 cut(s) 1018
VpaK11BI GGWCC 3 cut(s) 94, 280, 864
XagI CCTNNNNNAGG 1 cut(s) 609
XapI RAATTY 2 cut(s) 199, 550
XceI RCATGY 1 cut(s) 984
XmiI GTMKAC 1 cut(s) 53
XmnI GAANNNNTTC 2 cut(s) 600, 891
XspI CTAG 2 cut(s) 20, 129
ZraI GACGTC 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.