pycom15g24530

Cyclin-dependent kinase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009816
Physical Location & Seq
Forward (+)
18753920 .. 18754989
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGAGGAAGCAACAGCACGAAGATCTTGCAGAAGAGGAATTTACGACGATGGAGGTGACTCGAGCTGCCACGTCAGAGACGGCGGCGAGAAAGAGGAAGGCCGGCAGCGCGGACGGAGAATCAGTCGAATTGCCGTCGTCGTATGATGTGGCTCAGCACAAGAAGAGGCCGAGAAGACCCGTCGTCGTGGTTAGCAGCGCGCCGAAAATCAAGACGGAGGCAGAGAGCGTACGGACTTCCAACGACGACGTTTGGACGTCGGATCACGCGGAGAGCTCCTGCTGCTCGAGTAACGGCTCGAGCGAGCTGGAGGATGACAGTGACCAGGTCGAATCGTGGACGTATAATTCCAGCAGAGACGAAAGGAGAAAGATGACGGCGCCGACGAGCGAAGTTGGAGCAGAAGCAGATTCGACGGCGAGGCTTAAAGAGGAGTCTCAACGACGATCACCGGCGGTAGTGAACGCGTCGGAGCTCGAAGAATTCTTCGCTGCCGTGGAAAAGGAAAGCCAGCAAAAATTTAACGAGAAGTACAATTTTGATGTTTCTAAGGACGAACCGCTCGAAGGACGTTACGAGTGGGTTCGATTAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDI

Protein Analysis

200

Amino Acids

22.43

Weight (kDa)

4.95

Isoelectric Point (pI)

63.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDI PF02234 154 - 197 1.1e-16 Cyclin-dependent kinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 562
AccII CGCG 4 cut(s) 110, 200, 269, 467
AciI CCGC 5 cut(s) 83, 110, 269, 455, 560
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 3 cut(s) 38, 482, 518
AcyI GRCGYC 2 cut(s) 257, 380
AfaI GTAC 2 cut(s) 231, 533
AfiI CCNNNNNNNGG 1 cut(s) 566
AflIII ACRYGT 1 cut(s) 465
AhdI GACNNNNNGTC 1 cut(s) 182
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 4 cut(s) 65, 276, 307, 475
AluI AGCT 4 cut(s) 65, 276, 307, 475
Alw21I GWGCWC 2 cut(s) 278, 477
Alw26I GTCTC 3 cut(s) 71, 351, 441
AlwI GGATC 1 cut(s) 270
Ama87I CYCGRG 3 cut(s) 60, 286, 298
AoxI GGCC 2 cut(s) 99, 167
ApeKI GCWGC 5 cut(s) 65, 105, 195, 282, 491
ApoI RAATTY 3 cut(s) 38, 482, 518
AspLEI GCGC 4 cut(s) 110, 200, 202, 382
AsuHPI GGTGA 2 cut(s) 67, 441
AvaI CYCGRG 3 cut(s) 60, 286, 298
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 2 cut(s) 278, 477
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 2 cut(s) 278, 477
BbvI GCAGC 5 cut(s) 52, 117, 207, 269, 478
BccI CCATC 1 cut(s) 43
BceAI ACGGC 6 cut(s) 96, 118, 310, 393, 432, 479
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 3 cut(s) 71, 351, 441
BfoI RGCGCY 1 cut(s) 383
BglII AGATCT 1 cut(s) 22
BisI GCNGC 6 cut(s) 66, 84, 106, 196, 283, 492
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 6 cut(s) 67, 85, 107, 197, 284, 493
Bme1390I CCNGG 1 cut(s) 326
BmeRI GACNNNNNGTC 1 cut(s) 182
BmeT110I CYCGRG 3 cut(s) 60, 286, 298
BmgBI CACGTC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 257, 380
BsaJI CCNNGG 1 cut(s) 495
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 566
Bse118I RCCGGY 2 cut(s) 101, 451
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 566
BseMII CTCAG 1 cut(s) 167
BsePI GCGCGC 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 5 cut(s) 52, 117, 207, 269, 478
Bsh1236I CGCG 4 cut(s) 110, 200, 269, 467
BshFI GGCC 2 cut(s) 101, 169
BshNI GGYRCC 1 cut(s) 379
BsiHKAI GWGCWC 2 cut(s) 278, 477
BsiHKCI CYCGRG 3 cut(s) 60, 286, 298
BsiSI CCGG 2 cut(s) 102, 452
BsiWI CGTACG 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 566
BsmAI GTCTC 3 cut(s) 71, 351, 441
BsmBI CGTCTC 2 cut(s) 71, 351
BsnI GGCC 2 cut(s) 101, 169
BsoBI CYCGRG 3 cut(s) 60, 286, 298
Bsp1286I GDGCHC 2 cut(s) 278, 477
Bsp143I GATC 3 cut(s) 22, 262, 446
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 5 cut(s) 83, 110, 269, 455, 560
BspANI GGCC 2 cut(s) 101, 169
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 4 cut(s) 110, 200, 269, 467
BspLI GGNNCC 1 cut(s) 381
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 562
BsrFI RCCGGY 2 cut(s) 101, 451
BssAI RCCGGY 2 cut(s) 101, 451
BssECI CCNNGG 1 cut(s) 495
BssHII GCGCGC 1 cut(s) 198
BssMI GATC 3 cut(s) 22, 262, 446
BssNI GRCGYC 2 cut(s) 257, 380
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 2 cut(s) 27, 158
BstACI GRCGYC 2 cut(s) 257, 380
BstC8I GCNNGC 4 cut(s) 103, 200, 305, 512
BstDEI CTNAG 2 cut(s) 153, 549
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 4 cut(s) 110, 200, 269, 467
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 4 cut(s) 110, 200, 202, 382
BstKTI GATC 3 cut(s) 25, 265, 449
BstMAI GTCTC 3 cut(s) 71, 351, 441
BstMBI GATC 3 cut(s) 22, 262, 446
BstMWI GCNNNNNNNGC 2 cut(s) 107, 282
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 4 cut(s) 110, 200, 269, 467
BstV1I GCAGC 5 cut(s) 52, 117, 207, 269, 478
BstV2I GAAGAC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 2 cut(s) 101, 169
BtgI CCRYGG 1 cut(s) 495
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 4 cut(s) 103, 200, 305, 512
CfoI GCGC 4 cut(s) 110, 200, 202, 382
Cfr10I RCCGGY 2 cut(s) 101, 451
CseI GACGC 1 cut(s) 456
CsiI ACCWGGT 1 cut(s) 324
Csp6I GTAC 2 cut(s) 230, 532
CviAII CATG 1 cut(s) 597
CviQI GTAC 2 cut(s) 230, 532
DdeI CTNAG 2 cut(s) 153, 549
DinI GGCGCC 1 cut(s) 381
DpnI GATC 3 cut(s) 24, 264, 448
DpnII GATC 3 cut(s) 22, 262, 446
DriI GACNNNNNGTC 1 cut(s) 182
Eam1104I CTCTTC 2 cut(s) 27, 158
Eam1105I GACNNNNNGTC 1 cut(s) 182
EarI CTCTTC 2 cut(s) 27, 158
Ecl136II GAGCTC 2 cut(s) 276, 475
Eco24I GRGCYC 2 cut(s) 278, 477
Eco53kI GAGCTC 2 cut(s) 276, 475
Eco88I CYCGRG 3 cut(s) 60, 286, 298
EcoICRI GAGCTC 2 cut(s) 276, 475
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 278, 477
EgeI GGCGCC 1 cut(s) 381
EheI GGCGCC 1 cut(s) 381
Esp3I CGTCTC 2 cut(s) 71, 351
FaeI CATG 1 cut(s) 600
FaiI YATR 3 cut(s) 144, 345, 598
FatI CATG 1 cut(s) 596
Fnu4HI GCNGC 6 cut(s) 66, 84, 106, 196, 283, 492
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 2 cut(s) 278, 477
Fsp4HI GCNGC 6 cut(s) 66, 84, 106, 196, 283, 492
GlaI GCGC 4 cut(s) 109, 199, 201, 381
GluI GCNGC 6 cut(s) 66, 84, 106, 196, 283, 492
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 2 cut(s) 101, 169
HapII CCGG 2 cut(s) 102, 452
HgaI GACGC 1 cut(s) 456
HhaI GCGC 4 cut(s) 110, 200, 202, 382
Hin1I GRCGYC 2 cut(s) 257, 380
Hin1II CATG 1 cut(s) 600
Hin6I GCGC 4 cut(s) 108, 198, 200, 380
HinP1I GCGC 4 cut(s) 108, 198, 200, 380
HinfI GANTC 5 cut(s) 58, 119, 332, 410, 434
HpaII CCGG 2 cut(s) 102, 452
HphI GGTGA 2 cut(s) 67, 441
Hpy166II GTNNAC 2 cut(s) 339, 463
Hpy188I TCNGA 3 cut(s) 76, 262, 472
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 2 cut(s) 339, 463
HpyAV CCTTC 2 cut(s) 91, 560
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 5 cut(s) 71, 249, 257, 341, 571
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 282
HpyF3I CTNAG 2 cut(s) 153, 549
HpySE526I ACGT 5 cut(s) 71, 249, 257, 341, 571
Hsp92I GRCGYC 2 cut(s) 257, 380
Hsp92II CATG 1 cut(s) 600
HspAI GCGC 4 cut(s) 108, 198, 200, 380
KasI GGCGCC 1 cut(s) 379
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 3 cut(s) 22, 262, 446
LmnI GCTCC 3 cut(s) 281, 398, 472
LpnPI CCDG 8 cut(s) 115, 292, 293, 311, 338, 364, 465, 524
Lsp1109I GCAGC 5 cut(s) 52, 117, 207, 269, 478
MabI ACCWGGT 1 cut(s) 324
MaeII ACGT 5 cut(s) 71, 249, 257, 341, 571
MaeIII GTNAC 4 cut(s) 55, 290, 320, 572
MalI GATC 3 cut(s) 24, 264, 448
MbiI CCGCTC 1 cut(s) 562
MboI GATC 3 cut(s) 22, 262, 446
MboII GAAGA 6 cut(s) 32, 44, 175, 186, 478, 491
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 2 cut(s) 278, 477
MluCI AATT 6 cut(s) 38, 128, 346, 482, 518, 535
MluI ACGCGT 1 cut(s) 465
Mly113I GGCGCC 1 cut(s) 380
MlyI GAGTC 2 cut(s) 52, 443
MmeI TCCRAC 4 cut(s) 240, 264, 376, 450
MnlI CCTC 8 cut(s) 28, 46, 87, 159, 211, 304, 414, 424
MroNI GCCGGC 1 cut(s) 101
MseI TTAA 3 cut(s) 426, 522, 590
MspI CCGG 2 cut(s) 102, 452
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 4 cut(s) 110, 200, 269, 467
MwoI GCNNNNNNNGC 2 cut(s) 107, 282
NaeI GCCGGC 1 cut(s) 103
NarI GGCGCC 1 cut(s) 380
NdeII GATC 3 cut(s) 22, 262, 446
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 600
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 55, 320
PaeR7I CTCGAG 3 cut(s) 60, 286, 298
PauI GCGCGC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 3 cut(s) 300, 383, 561
PdiI GCCGGC 1 cut(s) 103
PfeI GAWTC 3 cut(s) 119, 332, 410
Pfl23II CGTACG 1 cut(s) 229
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 6 cut(s) 67, 85, 107, 197, 284, 493
PleI GAGTC 2 cut(s) 52, 442
PluTI GGCGCC 1 cut(s) 383
PpsI GAGTC 2 cut(s) 52, 442
Psp124BI GAGCTC 2 cut(s) 278, 477
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspLI CGTACG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 381
PspXI VCTCGAGB 3 cut(s) 60, 286, 298
PsuI RGATCY 1 cut(s) 22
PsyI GACNNNGTC 1 cut(s) 326
PteI GCGCGC 1 cut(s) 198
RsaI GTAC 2 cut(s) 231, 533
RsaNI GTAC 2 cut(s) 230, 532
SacI GAGCTC 2 cut(s) 278, 477
SaqAI TTAA 3 cut(s) 426, 522, 590
SatI GCNGC 6 cut(s) 66, 84, 106, 196, 283, 492
Sau3AI GATC 3 cut(s) 22, 262, 446
SchI GAGTC 2 cut(s) 52, 443
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 278, 477
SexAI ACCWGGT 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 381
Sfr274I CTCGAG 3 cut(s) 60, 286, 298
SgrAI CRCCGGYG 1 cut(s) 451
SlaI CTCGAG 3 cut(s) 60, 286, 298
SmlI CTYRAG 3 cut(s) 60, 286, 298
SmoI CTYRAG 3 cut(s) 60, 286, 298
Sse9I AATT 6 cut(s) 38, 128, 346, 482, 518, 535
SsiI CCGC 5 cut(s) 83, 110, 269, 455, 560
SspDI GGCGCC 1 cut(s) 379
SstI GAGCTC 2 cut(s) 278, 477
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 5 cut(s) 74, 252, 260, 344, 574
TaqI TCGA 9 cut(s) 61, 126, 287, 299, 330, 413, 477, 564, 586
TasI AATT 6 cut(s) 38, 128, 346, 482, 518, 535
TatI WGTACW 1 cut(s) 531
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 119, 332, 410
Tru1I TTAA 3 cut(s) 426, 522, 590
Tru9I TTAA 3 cut(s) 426, 522, 590
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 2 cut(s) 55, 320
TseI GCWGC 5 cut(s) 65, 105, 195, 282, 491
Tsp45I GTSAC 2 cut(s) 55, 320
TspGWI ACGGA 3 cut(s) 129, 230, 247
TspRI CASTG 1 cut(s) 325
Tth111I GACNNNGTC 1 cut(s) 326
XapI RAATTY 3 cut(s) 38, 482, 518
XhoI CTCGAG 3 cut(s) 60, 286, 298
ZraI GACGTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.