Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0011728

Physical Location & Seq

Genomic Coordinates Forward (+)

19476688 .. 19479209

Gene Structure 2522 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGGCATCAACAATGTCTCTCGCCGTACTCACCATCCTCTTCCTCTCCTTTACCCTCTCATACTCCTCCCCGATCGTCGACTCTCAGAGCGAGGCCGACGTCAGAGCCATCTACCAAGAGTGGCTCGTGAAGCACCAAAAGACGTACAACGGCATCGGGGAGGAGGAGAGGAGGTTCGAGATCTTCAAGGACAACTTGAAGTTCATCGACGAGCATAATGCACAGGACCGTCCGTACAAGGTCGGTCTGAACGCTTTTGCTGACCTGACGAACCAAGAGTACCGTGCCAAGTTTTTGGGCACCAGGAGTGACCCTAAGCGCAGGGTCATGAAAGCCAAGAAGCCGAGCCTGAGGTACGCTTTTCGCGCCGGTGAAACGTTGCCGGAATCTGTGGATTGGAGAGTCAAGGGTGCCGTCAATCCCATAAAAAATCAAGGAAGTTGCGGAAGTTGCTGGGCTTTCTCGACAGTGGCCGCGGTGGAGGGCATAAACCAAATAGTCACAGGAGAGTTGGTGTCTCTCTCAGAGCAAGAGCTTGTAGACTGCGACAGATCCTACAACGCCGGCTGCAATGGTGGTCTCATGGACTACGCTTTCGAGTTCATCATCCAAAACGGCGGTATGGACACTGAATCGGACTACCCTTACAAAGCATATGACCAAAAATGCAACGTTGCACTGGAGAACAATAAGGTTGTTAGCATTGATGGGTACGAAGATGTTCCGTCCTATGACGAGAACGCTTTGAAGAAAGCCGTTGCACACCAACCCGTTAGCGTGGCCATTGAAGCCGGCGGCAGGGCCCTACAACTCTACGAGTCGGGTGTTTTCACTGGTGAGTGCGGGTCAGCATTGGACCATGGTGTCGTTGCTGTTGGGTATGGCACCGAGAACGGTACAGACTATTGGTTGGTGAGGAACTCGTGGGGCACCAACTGGGGAGAGAGTGGATATTTTAAGATAGAGAGAAATGTTGCAAGCACATACACAGGCAAGTGTGGAATTGCAATGGAGGCTTCTTACCCTACAAAGAAGGACCAAAACCACCCATCAGGCTCTTTCTTTGCTGAGGAAGACATCAAGATGGTCACAAGTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

367

Amino Acids

40.53

Weight (kDa)

5.05

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	38 - 94	2.6e-19	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	127 - 343	2e-86	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19400 AT3G19400
fragaria_vesca	FvH4_1g16530
malus_domestica	MD02G1177700.v1.1 MD15G1287500.v1.1
prunus_persica	Prupe.6G230400_v2.0.a1 Prupe.6G230400_v2.0.a1
pyrus_communis	pycom02g14370 pycom15g25110
rosa_chinensis	RchiOBHm_Chr2g0106431
rosa_laevigata	RLG00000017497
rosa_multiflora	Rmu_sc0004015.1_g000003
rosa_roxburghii	Rroxscaffold_2G00137190
rosa_rugosa	Rorug02G0135300
rosa_samantha	Rh2AG186600 Rh2BG196900 Rh2CG191600 Rh2DG192700
rosa_wichuraiana	Rw2G014540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	863
AatII	GACGTC	1 cut(s)	102
AccB1I	GGYRCC	4 cut(s)	299, 410, 884, 929
AccI	GTMKAC	2 cut(s)	78, 540
AccII	CGCG	2 cut(s)	366, 476
AciI	CCGC	6 cut(s)	444, 474, 476, 618, 795, 843
AclI	AACGTT	2 cut(s)	377, 672
AclWI	GGATC	1 cut(s)	546
AcoI	YGGCCR	2 cut(s)	471, 780
AcyI	GRCGYC	1 cut(s)	99
AfaI	GTAC	7 cut(s)	27, 146, 236, 281, 356, 713, 898
AfiI	CCNNNNNNNGG	1 cut(s)	798
AgsI	TTSAA	4 cut(s)	187, 199, 748, 788
AjnI	CCWGG	1 cut(s)	302
AloI	GAACNNNNNNTCC	2 cut(s)	158, 190
AluBI	AGCT	1 cut(s)	535
AluI	AGCT	1 cut(s)	535
Alw26I	GTCTC	3 cut(s)	21, 522, 584
AlwI	GGATC	1 cut(s)	546
AoxI	GGCC	4 cut(s)	93, 471, 780, 801
ApaI	GGGCCC	1 cut(s)	805
ApeKI	GCWGC	1 cut(s)	567
ArsI	GACNNNNNNTTYG	2 cut(s)	578, 610
Asp700I	GAANNNNTTC	1 cut(s)	720
AspLEI	GCGC	2 cut(s)	321, 368
AspS9I	GGNCC	5 cut(s)	226, 801, 802, 856, 1036
AsuHPI	GGTGA	4 cut(s)	22, 383, 848, 925
AvaII	GGWCC	3 cut(s)	226, 856, 1036
AxyI	CCTNAGG	1 cut(s)	350
BaeGI	GKGCMC	3 cut(s)	302, 805, 932
BalI	TGGCCA	1 cut(s)	782
BanI	GGYRCC	4 cut(s)	299, 410, 884, 929
BanII	GRGCYC	1 cut(s)	805
BauI	CACGAG	2 cut(s)	125, 922
BbsI	GAAGAC	1 cut(s)	1080
BbvCI	CCTCAGC	1 cut(s)	1068
BbvI	GCAGC	1 cut(s)	554
BccI	CCATC	5 cut(s)	41, 116, 701, 1057, 1078
BceAI	ACGGC	5 cut(s)	8, 166, 398, 631, 740
BcgI	CGANNNNNNTGC	2 cut(s)	200, 234
BciT130I	CCWGG	1 cut(s)	304
BcoDI	GTCTC	3 cut(s)	21, 522, 584
BglII	AGATCT	1 cut(s)	180
BisI	GCNGC	3 cut(s)	474, 568, 796
BlsI	GCNGC	3 cut(s)	475, 569, 797
Bme1390I	CCNGG	1 cut(s)	304
Bme18I	GGWCC	3 cut(s)	226, 856, 1036
BmgT120I	GGNCC	5 cut(s)	226, 801, 802, 856, 1036
BmiI	GGNNCC	5 cut(s)	301, 412, 803, 886, 931
BmrFI	CCNGG	1 cut(s)	304
BmrI	ACTGGG	1 cut(s)	946
BmsI	GCATC	2 cut(s)	14, 162
BmuI	ACTGGG	1 cut(s)	946
BpiI	GAAGAC	1 cut(s)	1080
BpmI	CTGGAG	1 cut(s)	701
Bpu10I	CCTNAGC	2 cut(s)	315, 1068
BsaHI	GRCGYC	1 cut(s)	99
BsaI	GGTCTC	1 cut(s)	584
BsaJI	CCNNGG	2 cut(s)	474, 859
BsaXI	ACNNNNNCTCC	4 cut(s)	158, 188, 498, 528
Bsc4I	CCNNNNNNNGG	1 cut(s)	798
Bse118I	RCCGGY	3 cut(s)	368, 563, 791
Bse1I	ACTGG	3 cut(s)	684, 838, 941
Bse21I	CCTNAGG	1 cut(s)	350
Bse3DI	GCAATG	2 cut(s)	577, 1014
BseBI	CCWGG	1 cut(s)	304
BseDI	CCNNGG	2 cut(s)	474, 859
BseGI	GGATG	2 cut(s)	33, 606
BseLI	CCNNNNNNNGG	1 cut(s)	798
BseMI	GCAATG	2 cut(s)	577, 1014
BseMII	CTCAG	4 cut(s)	98, 341, 537, 1059
BseNI	ACTGG	3 cut(s)	684, 838, 941
BseRI	GAGGAG	4 cut(s)	55, 176, 179, 184
BseSI	GKGCMC	3 cut(s)	302, 805, 932
BseXI	GCAGC	1 cut(s)	554
BseYI	CCCAGC	1 cut(s)	453
Bsh1236I	CGCG	2 cut(s)	366, 476
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	4 cut(s)	95, 473, 782, 803
BshNI	GGYRCC	4 cut(s)	299, 410, 884, 929
BsiEI	CGRYCG	1 cut(s)	75
BsiSI	CCGG	4 cut(s)	369, 383, 564, 792
BslI	CCNNNNNNNGG	1 cut(s)	798
BsmAI	GTCTC	3 cut(s)	21, 522, 584
BsnI	GGCC	4 cut(s)	95, 473, 782, 803
Bso31I	GGTCTC	1 cut(s)	584
Bsp120I	GGGCCC	1 cut(s)	801
Bsp1286I	GDGCHC	3 cut(s)	302, 805, 932
Bsp143I	GATC	3 cut(s)	72, 180, 551
Bsp19I	CCATGG	1 cut(s)	859
BspACI	CCGC	6 cut(s)	444, 474, 476, 618, 795, 843
BspANI	GGCC	4 cut(s)	95, 473, 782, 803
BspCNI	CTCAG	4 cut(s)	97, 342, 536, 1060
BspFNI	CGCG	2 cut(s)	366, 476
BspHI	TCATGA	1 cut(s)	327
BspLI	GGNNCC	5 cut(s)	301, 412, 803, 886, 931
BspPI	GGATC	1 cut(s)	546
BspT107I	GGYRCC	4 cut(s)	299, 410, 884, 929
BspTNI	GGTCTC	1 cut(s)	584
BsrDI	GCAATG	2 cut(s)	577, 1014
BsrFI	RCCGGY	3 cut(s)	368, 563, 791
BsrI	ACTGG	3 cut(s)	684, 838, 941
BssAI	RCCGGY	3 cut(s)	368, 563, 791
BssECI	CCNNGG	2 cut(s)	474, 859
BssMI	GATC	3 cut(s)	72, 180, 551
BssNI	GRCGYC	1 cut(s)	99
BssSI	CACGAG	2 cut(s)	125, 922
BssT1I	CCWWGG	1 cut(s)	859
Bst2BI	CACGAG	2 cut(s)	125, 922
Bst2UI	CCWGG	1 cut(s)	304
Bst4CI	ACNGT	4 cut(s)	230, 284, 469, 896
Bst6I	CTCTTC	1 cut(s)	44
BstACI	GRCGYC	1 cut(s)	99
BstC8I	GCNNGC	3 cut(s)	565, 793, 979
BstDEI	CTNAG	5 cut(s)	84, 315, 350, 523, 1068
BstDSI	CCRYGG	2 cut(s)	474, 859
BstF5I	GGATG	2 cut(s)	33, 606
BstFNI	CGCG	2 cut(s)	366, 476
BstHHI	GCGC	2 cut(s)	321, 368
BstKTI	GATC	3 cut(s)	75, 183, 554
BstMAI	GTCTC	3 cut(s)	21, 522, 584
BstMBI	GATC	3 cut(s)	72, 180, 551
BstMCI	CGRYCG	1 cut(s)	75
BstMWI	GCNNNNNNNGC	5 cut(s)	130, 365, 450, 788, 1013
BstNI	CCWGG	1 cut(s)	304
BstSCI	CCNGG	1 cut(s)	302
BstSLI	GKGCMC	3 cut(s)	302, 805, 932
BstUI	CGCG	2 cut(s)	366, 476
BstV1I	GCAGC	1 cut(s)	554
BstV2I	GAAGAC	1 cut(s)	1080
BstX2I	RGATCY	2 cut(s)	180, 551
BstXI	CCANNNNNNTGG	1 cut(s)	295
BstYI	RGATCY	2 cut(s)	180, 551
Bsu36I	CCTNAGG	1 cut(s)	350
BsuRI	GGCC	4 cut(s)	95, 473, 782, 803
BtgI	CCRYGG	2 cut(s)	474, 859
BtsCI	GGATG	2 cut(s)	33, 606
BtsIMutI	CAGTG	4 cut(s)	474, 627, 677, 831
Cac8I	GCNNGC	3 cut(s)	565, 793, 979
CciI	TCATGA	1 cut(s)	327
CfoI	GCGC	2 cut(s)	321, 368
Cfr10I	RCCGGY	3 cut(s)	368, 563, 791
Cfr13I	GGNCC	5 cut(s)	226, 801, 802, 856, 1036
Cfr42I	CCGCGG	1 cut(s)	477
Csp6I	GTAC	7 cut(s)	26, 145, 235, 280, 355, 712, 897
CviAII	CATG	4 cut(s)	328, 583, 860, 1098
CviQI	GTAC	7 cut(s)	26, 145, 235, 280, 355, 712, 897
DdeI	CTNAG	5 cut(s)	84, 315, 350, 523, 1068
DpnI	GATC	3 cut(s)	74, 182, 553
DpnII	GATC	3 cut(s)	72, 180, 551
DrdI	GACNNNNNNGTC	1 cut(s)	863
DseDI	GACNNNNNNGTC	1 cut(s)	863
EaeI	YGGCCR	2 cut(s)	471, 780
Eam1104I	CTCTTC	1 cut(s)	44
EarI	CTCTTC	1 cut(s)	44
Eco130I	CCWWGG	1 cut(s)	859
Eco24I	GRGCYC	1 cut(s)	805
Eco31I	GGTCTC	1 cut(s)	584
Eco47I	GGWCC	3 cut(s)	226, 856, 1036
Eco81I	CCTNAGG	1 cut(s)	350
EcoO109I	RGGNCCY	2 cut(s)	801, 802
EcoRII	CCWGG	1 cut(s)	302
EcoT14I	CCWWGG	1 cut(s)	859
EcoT38I	GRGCYC	1 cut(s)	805
ErhI	CCWWGG	1 cut(s)	859
FaeI	CATG	4 cut(s)	331, 586, 863, 1101
FalI	AAGNNNNNCTT	2 cut(s)	179, 211
FatI	CATG	4 cut(s)	327, 582, 859, 1097
FauI	CCCGC	1 cut(s)	836
FauNDI	CATATG	1 cut(s)	655
FblI	GTMKAC	2 cut(s)	78, 540
Fnu4HI	GCNGC	3 cut(s)	474, 568, 796
FokI	GGATG	2 cut(s)	20, 593
FriOI	GRGCYC	1 cut(s)	805
Fsp4HI	GCNGC	3 cut(s)	474, 568, 796
GlaI	GCGC	2 cut(s)	320, 367
GluI	GCNGC	3 cut(s)	474, 568, 796
GsaI	CCCAGC	1 cut(s)	457
GsuI	CTGGAG	1 cut(s)	701
HaeIII	GGCC	4 cut(s)	95, 473, 782, 803
HapII	CCGG	4 cut(s)	369, 383, 564, 792
HhaI	GCGC	2 cut(s)	321, 368
Hin1I	GRCGYC	1 cut(s)	99
Hin1II	CATG	4 cut(s)	331, 586, 863, 1101
Hin6I	GCGC	2 cut(s)	319, 366
HinP1I	GCGC	2 cut(s)	319, 366
HincII	GTYRAC	1 cut(s)	79
HindII	GTYRAC	1 cut(s)	79
HinfI	GANTC	5 cut(s)	80, 386, 402, 632, 818
HpaII	CCGG	4 cut(s)	369, 383, 564, 792
HphI	GGTGA	4 cut(s)	22, 383, 848, 925
Hpy166II	GTNNAC	2 cut(s)	79, 541
Hpy188I	TCNGA	5 cut(s)	87, 104, 249, 526, 637
Hpy188III	TCNNGA	5 cut(s)	127, 178, 328, 463, 1081
Hpy8I	GTNNAC	2 cut(s)	79, 541
Hpy99I	CGWCG	3 cut(s)	80, 101, 212
HpyAV	CCTTC	1 cut(s)	1027
HpyCH4III	ACNGT	4 cut(s)	230, 284, 469, 896
HpyCH4IV	ACGT	4 cut(s)	99, 143, 377, 672
HpyCH4V	TGCA	8 cut(s)	221, 570, 669, 677, 761, 977, 1007, 1097
HpyF10VI	GCNNNNNNNGC	5 cut(s)	130, 365, 450, 788, 1013
HpyF3I	CTNAG	5 cut(s)	84, 315, 350, 523, 1068
HpySE526I	ACGT	4 cut(s)	99, 143, 377, 672
Hsp92I	GRCGYC	1 cut(s)	99
Hsp92II	CATG	4 cut(s)	331, 586, 863, 1101
HspAI	GCGC	2 cut(s)	319, 366
KroI	GCCGGC	2 cut(s)	563, 791
KroNI	GCCGGC	2 cut(s)	565, 793
KspI	CCGCGG	1 cut(s)	477
Kzo9I	GATC	3 cut(s)	72, 180, 551
Lsp1109I	GCAGC	1 cut(s)	554
LweI	GCATC	2 cut(s)	14, 162
MaeII	ACGT	4 cut(s)	99, 143, 377, 672
MaeIII	GTNAC	3 cut(s)	308, 499, 1087
MalI	GATC	3 cut(s)	74, 182, 553
MboI	GATC	3 cut(s)	72, 180, 551
MboII	GAAGA	5 cut(s)	31, 175, 728, 760, 1085
MflI	RGATCY	2 cut(s)	180, 551
MhlI	GDGCHC	3 cut(s)	302, 805, 932
MlsI	TGGCCA	1 cut(s)	782
MluCI	AATT	1 cut(s)	1002
MluNI	TGGCCA	1 cut(s)	782
MlyI	GAGTC	3 cut(s)	74, 411, 827
Mox20I	TGGCCA	1 cut(s)	782
MroNI	GCCGGC	2 cut(s)	563, 791
MroXI	GAANNNNTTC	1 cut(s)	720
MscI	TGGCCA	1 cut(s)	782
MseI	TTAA	1 cut(s)	957
MslI	CAYNNNNRTG	1 cut(s)	1082
Msp20I	TGGCCA	1 cut(s)	782
MspA1I	CMGCKG	1 cut(s)	476
MspI	CCGG	4 cut(s)	369, 383, 564, 792
MspR9I	CCNGG	1 cut(s)	304
MvaI	CCWGG	1 cut(s)	304
MvnI	CGCG	2 cut(s)	366, 476
MwoI	GCNNNNNNNGC	5 cut(s)	130, 365, 450, 788, 1013
NaeI	GCCGGC	2 cut(s)	565, 793
NcoI	CCATGG	1 cut(s)	859
NdeI	CATATG	1 cut(s)	655
NdeII	GATC	3 cut(s)	72, 180, 551
NgoMIV	GCCGGC	2 cut(s)	563, 791
NlaIII	CATG	4 cut(s)	331, 586, 863, 1101
NlaIV	GGNNCC	5 cut(s)	301, 412, 803, 886, 931
NmeAIII	GCCGAG	1 cut(s)	369
NmuCI	GTSAC	3 cut(s)	308, 499, 1087
PagI	TCATGA	1 cut(s)	327
PdiI	GCCGGC	2 cut(s)	565, 793
PdmI	GAANNNNTTC	1 cut(s)	720
PfeI	GAWTC	2 cut(s)	386, 632
PkrI	GCNGC	3 cut(s)	475, 569, 797
Ple19I	CGATCG	1 cut(s)	75
PleI	GAGTC	3 cut(s)	74, 410, 826
PpsI	GAGTC	3 cut(s)	74, 410, 826
Psp1406I	AACGTT	2 cut(s)	377, 672
Psp6I	CCWGG	1 cut(s)	302
PspFI	CCCAGC	1 cut(s)	453
PspGI	CCWGG	1 cut(s)	302
PspN4I	GGNNCC	5 cut(s)	301, 412, 803, 886, 931
PspOMI	GGGCCC	1 cut(s)	801
PspPI	GGNCC	5 cut(s)	226, 801, 802, 856, 1036
PsrI	GAACNNNNNNTAC	2 cut(s)	263, 295
PsuI	RGATCY	2 cut(s)	180, 551
PvuI	CGATCG	1 cut(s)	75
RsaI	GTAC	7 cut(s)	27, 146, 236, 281, 356, 713, 898
RsaNI	GTAC	7 cut(s)	26, 145, 235, 280, 355, 712, 897
RseI	CAYNNNNRTG	1 cut(s)	1082
SacII	CCGCGG	1 cut(s)	477
SalI	GTCGAC	1 cut(s)	77
SaqAI	TTAA	1 cut(s)	957
SatI	GCNGC	3 cut(s)	474, 568, 796
Sau3AI	GATC	3 cut(s)	72, 180, 551
Sau96I	GGNCC	5 cut(s)	226, 801, 802, 856, 1036
SchI	GAGTC	3 cut(s)	74, 411, 827
ScrFI	CCNGG	1 cut(s)	304
SduI	GDGCHC	3 cut(s)	302, 805, 932
SfaNI	GCATC	2 cut(s)	14, 162
Sfr303I	CCGCGG	1 cut(s)	477
SgrAI	CRCCGGYG	1 cut(s)	368
SgrBI	CCGCGG	1 cut(s)	477
SinI	GGWCC	3 cut(s)	226, 856, 1036
SmiMI	CAYNNNNRTG	1 cut(s)	1082
Sse9I	AATT	1 cut(s)	1002
SsiI	CCGC	6 cut(s)	444, 474, 476, 618, 795, 843
StyD4I	CCNGG	1 cut(s)	302
StyI	CCWWGG	1 cut(s)	859
TaaI	ACNGT	4 cut(s)	230, 284, 469, 896
TaiI	ACGT	4 cut(s)	102, 146, 380, 675
TaqI	TCGA	5 cut(s)	78, 177, 207, 464, 597
TaqII	GACCGA	1 cut(s)	233
TasI	AATT	1 cut(s)	1002
TauI	GCSGC	2 cut(s)	476, 798
TfiI	GAWTC	2 cut(s)	386, 632
Tru1I	TTAA	1 cut(s)	957
Tru9I	TTAA	1 cut(s)	957
TscAI	CASTG	4 cut(s)	474, 634, 684, 838
TseFI	GTSAC	3 cut(s)	308, 499, 1087
TseI	GCWGC	1 cut(s)	567
Tsp45I	GTSAC	3 cut(s)	308, 499, 1087
TspDTI	ATGAA	3 cut(s)	193, 344, 592
TspGWI	ACGGA	2 cut(s)	222, 714
TspRI	CASTG	4 cut(s)	474, 634, 684, 838
VpaK11BI	GGWCC	3 cut(s)	226, 856, 1036
XmiI	GTMKAC	2 cut(s)	78, 540
XmnI	GAANNNNTTC	1 cut(s)	720
ZraI	GACGTC	1 cut(s)	100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.