pycom15g25120

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004633
Physical Location & Seq
Forward (+)
19481696 .. 19482341
646 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGACAAAACCATTGTGCCTCAAATTGTGTCACTGGGGCCTTACCATCACGGCAAGAATGGACCGCCACAAATGGCGGTCGCTTCATCTAATGCCAAGGCGGACAAACCAGTTCATAGAGCTGTATCTTGATTCTATAAAAGAAGTTGAAGAAACGGCTCGAGCTTGCTATGAAGAGCCAGTTGCAATGGGGCTCAGCAGCAACGAGTTCGCAGAAATGATGCTGCTTGACGGTTGCTTTGTGCTCGAGCTGTTTCGAGGCACTGCCGAGGGATTGAAGGACATGATCATGCTTGAGAATCAGCTTCCGTTTTTCATTCTTTATCGGTTATTGGTGCTGCAGTATGGAGATCCTGAGCAGAAAGGACTTGTAGCTCAGCTAGACTTGGGTTTTTCGATCCATTGTAATCGCTTGGATACGCAACCACCTTCGATCCCGTCCGAACAAGGTGGCCCTCATTGTCTTGACGTGTTCAGGCGAAGTCTATTGCGCACGGGACTTCAGACTGCGCCGAGAGTTTGGATCAAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

178

Amino Acids

20.47

Weight (kDa)

6.95

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 18 - 165 3.8e-32 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
AciI CCGC 3 cut(s) 64, 76, 100
AclWI GGATC 4 cut(s) 344, 391, 427, 530
AcuI CTGAAG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 2 cut(s) 149, 277
AjiI CACGTC 1 cut(s) 469
AluBI AGCT 6 cut(s) 121, 164, 250, 304, 374, 379
AluI AGCT 6 cut(s) 121, 164, 250, 304, 374, 379
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 4 cut(s) 344, 391, 427, 530
Ama87I CYCGRG 2 cut(s) 159, 245
AoxI GGCC 2 cut(s) 37, 451
ApeKI GCWGC 3 cut(s) 198, 223, 337
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AspLEI GCGC 2 cut(s) 492, 511
AspS9I GGNCC 3 cut(s) 37, 61, 452
AvaI CYCGRG 2 cut(s) 159, 245
AvaII GGWCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 210, 210, 324
BccI CCATC 1 cut(s) 53
BceAI ACGGC 2 cut(s) 66, 171
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 1 cut(s) 285
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 338
BfuI GTATCC 1 cut(s) 409
BisI GCNGC 3 cut(s) 199, 224, 338
BlpI GCTNAGC 2 cut(s) 194, 375
BlsI GCNGC 3 cut(s) 200, 225, 339
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 159, 245
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 37, 61, 452
BmiI GGNNCC 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 43
BmsI GCATC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 2 cut(s) 194, 375
BpuEI CTTGAG 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 95, 267
Bse1I ACTGG 3 cut(s) 38, 109, 179
Bse3DI GCAATG 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 95, 267
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 3 cut(s) 208, 345, 389
BseNI ACTGG 3 cut(s) 38, 109, 179
BseXI GCAGC 3 cut(s) 210, 210, 324
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 2 cut(s) 39, 453
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 2 cut(s) 159, 245
BslFI GGGAC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 2 cut(s) 39, 453
BsoBI CYCGRG 2 cut(s) 159, 245
Bsp1286I GDGCHC 2 cut(s) 195, 246
Bsp143I GATC 5 cut(s) 285, 349, 396, 432, 522
Bsp1720I GCTNAGC 2 cut(s) 194, 375
BspACI CCGC 3 cut(s) 64, 76, 100
BspANI GGCC 2 cut(s) 39, 453
BspCNI CTCAG 3 cut(s) 207, 346, 388
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 342
BspPI GGATC 4 cut(s) 344, 391, 427, 530
BspQI GCTCTTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 3 cut(s) 38, 109, 179
BssECI CCNNGG 2 cut(s) 95, 267
BssMI GATC 5 cut(s) 285, 349, 396, 432, 522
BssT1I CCWWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 3 cut(s) 194, 354, 375
BstHHI GCGC 2 cut(s) 492, 511
BstKTI GATC 5 cut(s) 288, 352, 399, 435, 525
BstMBI GATC 5 cut(s) 285, 349, 396, 432, 522
BstMCI CGRYCG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 210, 210, 324
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 2 cut(s) 39, 453
BtrI CACGTC 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 31, 261
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 492, 511
Cfr13I GGNCC 3 cut(s) 37, 61, 452
CviAII CATG 2 cut(s) 283, 289
DdeI CTNAG 3 cut(s) 194, 354, 375
DpnI GATC 5 cut(s) 287, 351, 398, 434, 524
DpnII GATC 5 cut(s) 285, 349, 396, 432, 522
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 2 cut(s) 159, 245
EcoO109I RGGNCCY 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 2 cut(s) 286, 292
FaiI YATR 6 cut(s) 116, 137, 171, 284, 290, 345
FaqI GGGAC 1 cut(s) 510
FatI CATG 2 cut(s) 282, 288
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 3 cut(s) 199, 224, 338
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 199, 224, 338
FspBI CTAG 1 cut(s) 380
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 2 cut(s) 491, 510
GluI GCNGC 3 cut(s) 199, 224, 338
HaeIII GGCC 2 cut(s) 39, 453
HhaI GCGC 2 cut(s) 492, 511
Hin1II CATG 2 cut(s) 286, 292
Hin6I GCGC 2 cut(s) 490, 509
HinP1I GCGC 2 cut(s) 490, 509
HinfI GANTC 2 cut(s) 131, 298
Hpy188I TCNGA 2 cut(s) 442, 504
Hpy188III TCNNGA 4 cut(s) 128, 353, 464, 526
HpyAV CCTTC 2 cut(s) 271, 438
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 2 cut(s) 185, 340
HpyF3I CTNAG 3 cut(s) 194, 354, 375
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 2 cut(s) 286, 292
HspAI GCGC 2 cut(s) 490, 509
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 5 cut(s) 285, 349, 396, 432, 522
LguI GCTCTTC 1 cut(s) 168
LpnPI CCDG 5 cut(s) 19, 122, 192, 366, 460
Lsp1109I GCAGC 3 cut(s) 210, 210, 324
LweI GCATC 1 cut(s) 210
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 29
MalI GATC 5 cut(s) 287, 351, 398, 434, 524
MboI GATC 5 cut(s) 285, 349, 396, 432, 522
MboII GAAGA 2 cut(s) 161, 185
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 2 cut(s) 195, 246
MluCI AATT 1 cut(s) 23
MnlI CCTC 4 cut(s) 29, 251, 262, 465
MslI CAYNNNNRTG 1 cut(s) 287
NdeII GATC 5 cut(s) 285, 349, 396, 432, 522
NlaIII CATG 2 cut(s) 286, 292
NlaIV GGNNCC 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 29
NsbI TGCGCA 1 cut(s) 491
PaeR7I CTCGAG 2 cut(s) 159, 245
PciSI GCTCTTC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 131, 298
PkrI GCNGC 3 cut(s) 200, 225, 339
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 3 cut(s) 37, 61, 452
PspXI VCTCGAGB 2 cut(s) 159, 245
PstI CTGCAG 1 cut(s) 342
PsuI RGATCY 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 168
SatI GCNGC 3 cut(s) 199, 224, 338
Sau3AI GATC 5 cut(s) 285, 349, 396, 432, 522
Sau96I GGNCC 3 cut(s) 37, 61, 452
SduI GDGCHC 2 cut(s) 195, 246
SetI ASST 9 cut(s) 123, 166, 252, 306, 376, 381, 430, 451, 471
SfaNI GCATC 1 cut(s) 210
SfcI CTRYAG 1 cut(s) 338
Sfr274I CTCGAG 2 cut(s) 159, 245
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 2 cut(s) 159, 245
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 3 cut(s) 159, 245, 293
SmoI CTYRAG 3 cut(s) 159, 245, 293
Sse9I AATT 1 cut(s) 23
SsiI CCGC 3 cut(s) 64, 76, 100
SspMI CTAG 1 cut(s) 380
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 1 cut(s) 471
TaqI TCGA 5 cut(s) 160, 246, 256, 395, 431
TasI AATT 1 cut(s) 23
TfiI GAWTC 2 cut(s) 131, 298
TscAI CASTG 2 cut(s) 38, 268
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 3 cut(s) 198, 223, 337
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 4 cut(s) 74, 103, 186, 304
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 2 cut(s) 38, 268
VpaK11BI GGWCC 1 cut(s) 61
XhoI CTCGAG 2 cut(s) 159, 245
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.