pycom15g25860

Belongs to the formin-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005682
Physical Location & Seq
Forward (+)
20525184 .. 20527030
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1470 bp
ATGGCTCCAACTAAGGAAGAAGAAATTAAACTTCGAGACTACAAGGGTGACGTCTCAAAATTAGGGACTGCTGAACGCTTTCTGAAAGCTGTGCTCGATATCCCATTTGCCTTTAAGAGAGTTGAGGCAATGCTATACAGAGCTAATTTTGATACAGAAGTGAAGTACCTGAGAAAGTCCTTTCAAACGTTGGAGGAAGCAAGTGATGAACTAAAGAGCAGTCGATTATTCCTCAAACTCCTTGAAGCTGTCCTCAAGACAGGGAACCGAATGAATGTTGGGACTAATCGTGGTGATGCTAAATCTTTCAAACTTGATACACTTTTAAAACTAGTTGACGTAAAGGCAACAGATGGAAAGACCACATTGCTCCATTTTGTGGTCCAAGAGATCATAAAATCCGAAGGTGAAGTTGTTGATTCTACAAATGAGAATGCTAAAAACATGCCTGTCAATACCAAGGAAGATGGTTTCAGAAAGCAAGGATTGCAAATGGTTGCAGGGCTGGGCAGAGACCTCAGCAACGTCAAGAAAGCAGCAGGAATGGACTCAGATGTCCTAAGTAGCTACGTGTCGAAGCTTGAAATGGGGCTTCAGAAGGTCAAACTGGTTTTGCAGTATGAGAAGCCCGATATGCAAGGGAAATTCTTCAACTCGATGAAGGGATTTCTAAAAGAGGCCGAGGATGAAATTCTTAGGGTCAAGGCTGATGAAAGAAAGGCTTTAATTCTGGTGAAAGAGGTTACCGAATATTTTCATGGGGATACAACAAAGGAGGAAGCCCATCCTTTCAGAATCTTCATGATCGTGAGAGATTTTCTAAATGTACTGGACCAAGTTTGCAAGGAAGTGGGGATGCTGCAGGATAGAACACTGGTGGGCTCTGCTAGATCATTCCGCATAACATCAACCGCTCCTCTACCAGTTCTCAGCAGGTATAATGTGAGACAAGATAACAGTTCGGATGATGAAAGCTTGTCACCAAAGAAACGCAGGAGGGAGGGCACTCTTGATACTCTTTCCAGTATAAAGAAAATCCCAATTCAGACGGAGAGATTCCCCAGTCAGTTTATCAGGGTAGGATTCTTCATCTTGATCCAGAGTCCCACACATTTTGAAGGGAAAAGCAGAATGTTTTGGTGGTGGTGTCTGCATTTATTTCACAAATTAAAATATAGTTCAATAATAATTAGTAGCTATGTTTATTCTTCAGTATTGTCTTGTTTTTATTGTTTGATGAAAATAATTGAGGGGGAGGAGGAGACATATTGTCAATGTCTTCATATTTGTGGTCAAACTTTAAGGACAAACCAAGCTCCACTTCTTAACACTAACCACTTTAAGGAAAGCAAACAAAGACTACATCCTTCTAAAGATCTAATCACCTTTATGAAAAAGAAAAATAATTCTAGAAATAATGAAACTGTTTGTTGTTACACTGAAGGGAATCGAGCTCTGTATGTATCTTAA

Protein Analysis

490

Amino Acids

56.15

Weight (kDa)

9.15

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 1 - 277 1.2e-78 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AatII GACGTC 1 cut(s) 54
Acc36I ACCTGC 1 cut(s) 924
AccB7I CCANNNNNTGG 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 914
AciI CCGC 2 cut(s) 898, 912
AclI AACGTT 1 cut(s) 188
AclWI GGATC 1 cut(s) 1090
AcsI RAATTY 2 cut(s) 644, 690
AcuI CTGAAG 3 cut(s) 578, 1194, 1461
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 167, 828
AfiI CCNNNNNNNGG 2 cut(s) 379, 1342
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 7 cut(s) 185, 245, 310, 584, 652, 1118, 1182
AhdI GACNNNNNGTC 1 cut(s) 1269
AhlI ACTAGT 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 1305, 1337
AluBI AGCT 9 cut(s) 89, 143, 248, 567, 580, 975, 1197, 1316, 1454
AluI AGCT 9 cut(s) 89, 143, 248, 567, 580, 975, 1197, 1316, 1454
Alw21I GWGCWC 2 cut(s) 96, 1456
Alw26I GTCTC 5 cut(s) 30, 58, 507, 940, 1256
AlwI GGATC 1 cut(s) 1090
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 2 cut(s) 536, 859
ApoI RAATTY 2 cut(s) 644, 690
Asp700I GAANNNNTTC 3 cut(s) 78, 647, 753
AspS9I GGNCC 2 cut(s) 382, 832
AsuHPI GGTGA 6 cut(s) 59, 305, 419, 745, 972, 1375
AvaII GGWCC 2 cut(s) 382, 832
BaeGI GKGCMC 1 cut(s) 1007
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BanII GRGCYC 2 cut(s) 884, 1456
BbsI GAAGAC 1 cut(s) 1271
Bbv12I GWGCWC 2 cut(s) 96, 1456
BbvCI CCTCAGC 1 cut(s) 518
BbvI GCAGC 2 cut(s) 548, 846
BccI CCATC 3 cut(s) 347, 461, 792
BciVI GTATCC 1 cut(s) 757
BcoDI GTCTC 5 cut(s) 30, 58, 507, 940, 1256
BcuI ACTAGT 1 cut(s) 331
BfaI CTAG 3 cut(s) 332, 888, 1410
BfmI CTRYAG 1 cut(s) 860
BfuAI ACCTGC 1 cut(s) 924
BfuI GTATCC 1 cut(s) 757
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 2 cut(s) 537, 860
BlsI GCNGC 2 cut(s) 538, 861
Bme18I GGWCC 2 cut(s) 382, 832
BmeRI GACNNNNNGTC 1 cut(s) 1269
BmgT120I GGNCC 2 cut(s) 382, 832
BmiI GGNNCC 2 cut(s) 6, 266
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 2 cut(s) 286, 846
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 1 cut(s) 1271
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 459, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 1342
Bse1I ACTGG 6 cut(s) 612, 834, 879, 923, 1023, 1062
Bse3DI GCAATG 2 cut(s) 135, 365
BseDI CCNNGG 2 cut(s) 459, 681
BseGI GGATG 5 cut(s) 691, 784, 861, 970, 1363
BseLI CCNNNNNNNGG 2 cut(s) 379, 1342
BseMI GCAATG 2 cut(s) 135, 365
BseMII CTCAG 4 cut(s) 161, 532, 564, 943
BseNI ACTGG 6 cut(s) 612, 834, 879, 923, 1023, 1062
BseRI GAGGAG 3 cut(s) 906, 1271, 1274
BseSI GKGCMC 1 cut(s) 1007
BseXI GCAGC 2 cut(s) 548, 846
BseYI CCCAGC 1 cut(s) 505
BshFI GGCC 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 96, 1456
BslFI GGGAC 3 cut(s) 79, 295, 1089
BslI CCNNNNNNNGG 2 cut(s) 379, 1342
BsmAI GTCTC 5 cut(s) 30, 58, 507, 940, 1256
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 3 cut(s) 79, 295, 1089
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 680
Bso31I GGTCTC 1 cut(s) 507
Bsp1286I GDGCHC 4 cut(s) 96, 884, 1007, 1456
Bsp143I GATC 5 cut(s) 390, 804, 890, 1095, 1375
BspACI CCGC 2 cut(s) 898, 912
BspANI GGCC 1 cut(s) 680
BspCNI CTCAG 4 cut(s) 162, 531, 563, 942
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 2 cut(s) 6, 266
BspMAI CTGCAG 1 cut(s) 864
BspMI ACCTGC 1 cut(s) 924
BspPI GGATC 1 cut(s) 1090
BspTNI GGTCTC 1 cut(s) 507
BsrBI CCGCTC 1 cut(s) 914
BsrDI GCAATG 2 cut(s) 135, 365
BsrI ACTGG 6 cut(s) 612, 834, 879, 923, 1023, 1062
BssECI CCNNGG 2 cut(s) 459, 681
BssMI GATC 5 cut(s) 390, 804, 890, 1095, 1375
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 959, 1426
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 571
BstDEI CTNAG 7 cut(s) 12, 170, 518, 550, 560, 695, 929
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 5 cut(s) 691, 784, 861, 970, 1363
BstKTI GATC 5 cut(s) 393, 807, 893, 1098, 1378
BstMAI GTCTC 5 cut(s) 30, 58, 507, 940, 1256
BstMBI GATC 5 cut(s) 390, 804, 890, 1095, 1375
BstMWI GCNNNNNNNGC 2 cut(s) 487, 634
BstNSI RCATGY 1 cut(s) 448
BstPI GGTNACC 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 860
BstSLI GKGCMC 1 cut(s) 1007
BstV1I GCAGC 2 cut(s) 548, 846
BstV2I GAAGAC 1 cut(s) 1271
BstX2I RGATCY 1 cut(s) 1375
BstYI RGATCY 1 cut(s) 1375
BsuI GTATCC 1 cut(s) 757
BsuRI GGCC 1 cut(s) 680
BtsCI GGATG 5 cut(s) 691, 784, 861, 970, 1363
BtsIMutI CAGTG 2 cut(s) 872, 1437
BveI ACCTGC 1 cut(s) 924
CciI TCATGA 1 cut(s) 801
Cfr13I GGNCC 2 cut(s) 382, 832
Csp6I GTAC 2 cut(s) 166, 827
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
CviAII CATG 3 cut(s) 445, 758, 802
CviQI GTAC 2 cut(s) 166, 827
DdeI CTNAG 7 cut(s) 12, 170, 518, 550, 560, 695, 929
DpnI GATC 5 cut(s) 392, 806, 892, 1097, 1377
DpnII GATC 5 cut(s) 390, 804, 890, 1095, 1375
DraI TTTAAA 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 1 cut(s) 1269
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1105I GACNNNNNGTC 1 cut(s) 1269
Ecl136II GAGCTC 1 cut(s) 1454
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 884, 1456
Eco31I GGTCTC 1 cut(s) 507
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 382, 832
Eco53kI GAGCTC 1 cut(s) 1454
Eco57I CTGAAG 3 cut(s) 578, 1194, 1461
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 1454
EcoO65I GGTNACC 1 cut(s) 742
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 884, 1456
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 3 cut(s) 448, 761, 805
FalI AAGNNNNNCTT 4 cut(s) 706, 738, 1305, 1337
FaqI GGGAC 3 cut(s) 79, 295, 1089
FatI CATG 3 cut(s) 444, 757, 801
Fnu4HI GCNGC 2 cut(s) 537, 860
FokI GGATG 5 cut(s) 698, 771, 868, 977, 1350
FriOI GRGCYC 2 cut(s) 884, 1456
Fsp4HI GCNGC 2 cut(s) 537, 860
FspBI CTAG 3 cut(s) 332, 888, 1410
GluI GCNGC 2 cut(s) 537, 860
GsaI CCCAGC 1 cut(s) 509
HaeIII GGCC 1 cut(s) 680
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 448, 761, 805
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 2 cut(s) 578, 973
HinfI GANTC 7 cut(s) 419, 548, 795, 1056, 1083, 1102, 1447
HphI GGTGA 6 cut(s) 59, 305, 419, 745, 972, 1375
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 8 cut(s) 84, 403, 476, 553, 597, 794, 964, 1047
Hpy188III TCNNGA 9 cut(s) 35, 256, 529, 802, 808, 1010, 1093, 1099, 1410
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 6 cut(s) 398, 592, 655, 1112, 1377, 1436
HpyCH4III ACNGT 2 cut(s) 959, 1426
HpyCH4IV ACGT 5 cut(s) 51, 188, 339, 525, 570
HpyCH4V TGCA 7 cut(s) 490, 500, 616, 637, 843, 862, 1153
HpyF10VI GCNNNNNNNGC 2 cut(s) 487, 634
HpyF3I CTNAG 7 cut(s) 12, 170, 518, 550, 560, 695, 929
HpySE526I ACGT 5 cut(s) 51, 188, 339, 525, 570
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 448, 761, 805
Kzo9I GATC 5 cut(s) 390, 804, 890, 1095, 1375
LmnI GCTCC 4 cut(s) 10, 375, 919, 1321
Lsp1109I GCAGC 2 cut(s) 548, 846
LweI GCATC 2 cut(s) 286, 846
MaeI CTAG 3 cut(s) 332, 888, 1410
MaeII ACGT 5 cut(s) 51, 188, 339, 525, 570
MaeIII GTNAC 4 cut(s) 47, 742, 978, 1433
MalI GATC 5 cut(s) 392, 806, 892, 1097, 1377
MbiI CCGCTC 1 cut(s) 914
MboI GATC 5 cut(s) 390, 804, 890, 1095, 1375
MboII GAAGA 8 cut(s) 29, 32, 476, 640, 790, 1078, 1200, 1271
MflI RGATCY 1 cut(s) 1375
MhlI GDGCHC 4 cut(s) 96, 884, 1007, 1456
MlyI GAGTC 2 cut(s) 542, 1111
MmeI TCCRAC 2 cut(s) 32, 171
MroXI GAANNNNTTC 3 cut(s) 78, 647, 753
MseI TTAA 9 cut(s) 27, 114, 326, 725, 1169, 1301, 1326, 1341, 1468
MslI CAYNNNNRTG 3 cut(s) 806, 1287, 1388
Mva1269I GAATGC 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 487, 634
NdeII GATC 5 cut(s) 390, 804, 890, 1095, 1375
NlaIII CATG 3 cut(s) 448, 761, 805
NlaIV GGNNCC 2 cut(s) 6, 266
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 2 cut(s) 47, 978
NspI RCATGY 1 cut(s) 448
PagI TCATGA 1 cut(s) 801
PctI GAATGC 1 cut(s) 439
PdmI GAANNNNTTC 3 cut(s) 78, 647, 753
PfeI GAWTC 5 cut(s) 419, 795, 1056, 1083, 1447
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 2 cut(s) 538, 861
PleI GAGTC 2 cut(s) 542, 1110
PpsI GAGTC 2 cut(s) 542, 1110
Ppu21I YACGTR 1 cut(s) 571
Psp124BI GAGCTC 1 cut(s) 1456
Psp1406I AACGTT 1 cut(s) 188
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 6, 266
PspPI GGNCC 2 cut(s) 382, 832
PstI CTGCAG 1 cut(s) 864
PsuI RGATCY 1 cut(s) 1375
RsaI GTAC 2 cut(s) 167, 828
RsaNI GTAC 2 cut(s) 166, 827
RseI CAYNNNNRTG 3 cut(s) 806, 1287, 1388
SacI GAGCTC 1 cut(s) 1456
SaqAI TTAA 9 cut(s) 27, 114, 326, 725, 1169, 1301, 1326, 1341, 1468
SatI GCNGC 2 cut(s) 537, 860
Sau3AI GATC 5 cut(s) 390, 804, 890, 1095, 1375
Sau96I GGNCC 2 cut(s) 382, 832
SchI GAGTC 2 cut(s) 542, 1111
SduI GDGCHC 4 cut(s) 96, 884, 1007, 1456
SfaNI GCATC 2 cut(s) 286, 846
SfcI CTRYAG 1 cut(s) 860
SinI GGWCC 2 cut(s) 382, 832
SmiMI CAYNNNNRTG 3 cut(s) 806, 1287, 1388
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 331
SsiI CCGC 2 cut(s) 898, 912
SspI AATATT 1 cut(s) 752
SspMI CTAG 3 cut(s) 332, 888, 1410
SstI GAGCTC 1 cut(s) 1456
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 959, 1426
TaiI ACGT 5 cut(s) 54, 191, 342, 528, 573
TaqI TCGA 6 cut(s) 34, 96, 223, 575, 656, 1450
TatI WGTACW 1 cut(s) 826
TfiI GAWTC 5 cut(s) 419, 795, 1056, 1083, 1447
Tru1I TTAA 9 cut(s) 27, 114, 326, 725, 1169, 1301, 1326, 1341, 1468
Tru9I TTAA 9 cut(s) 27, 114, 326, 725, 1169, 1301, 1326, 1341, 1468
TscAI CASTG 2 cut(s) 879, 1444
TseFI GTSAC 2 cut(s) 47, 978
TseI GCWGC 2 cut(s) 536, 859
Tsp45I GTSAC 2 cut(s) 47, 978
TspGWI ACGGA 1 cut(s) 1064
TspRI CASTG 2 cut(s) 879, 1444
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 382, 832
XapI RAATTY 2 cut(s) 644, 690
XbaI TCTAGA 1 cut(s) 1409
XceI RCATGY 1 cut(s) 448
XmnI GAANNNNTTC 3 cut(s) 78, 647, 753
XspI CTAG 3 cut(s) 332, 888, 1410
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.