pycom15g27840

CAAD domains of cyanobacterial aminoacyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014374
Physical Location & Seq
Reverse (-)
23368950 .. 23371469
2520 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGCAGCTCTGTACAGCCGCTCCCCAAGCTATCTCCAACCTCCCTAACCACGGCCACCGCCGCGACAACCGCCATCTCATCACAGACTCCACCACCAACCTCAGCTCGAAACCCTCACTCGTTCACAGACAAATCCCATTCTCACTCAACTCTTTCCGGCTTCACTCTCGATTGCTTTTGGCGAGAGCAGCAAAATCAGAAGAAACTCCAAGTGGGCCCAATCCTTATGCTGCCGAAGAGCGCAGCGGCGGCGTAATCTTGGAGGATGTTGTGCCCCCTGAAAAGAATCTGTACAATCAAACTTGGACTGAAGAAGAAGTTAAAGAAGATGCAAATGCGGACGCGGATGGCCAAATACAGATATTCGATTATTTCAGTAACCTCGACTTAAAGTTGGATAGTGAGGATACATACAGCCTTTTGCTATACGGTGGTGGTGCCTTGTTTGCCCTGTGGTTAGCATCAGCTGTTGTTGGAGCTATTGATTCTATTCCGTTGTTCCCGAAGTTGCTGGAAGTCGTCGGTCTTGGATACACCGTCTGGTTTAGCTCTCGTTATCTGATATTCAAGAAAAACAGAGATGAGTTGGTTACTAAAGTTCAAGTCCTTAAACAGCAGGTTCTGGGTTCGGACGATGACTGA

Protein Analysis

214

Amino Acids

23.72

Weight (kDa)

5.13

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 134 - 209 4.5e-26 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38100 AT4G38100
fragaria_vesca FvH4_1g19890
malus_domestica MD15G1315100.v1.1
prunus_persica Prupe.6G196600_v2.0.a1
pyrus_communis pycom15g27840
rosa_chinensis RchiOBHm_Chr2g0111711
rosa_laevigata RLG00000017919
rosa_multiflora Rmu_sc0000448.1_g000011
rosa_roxburghii Rroxscaffold_2G00132150
rosa_rugosa Rorug02G0175600 Rorug02G0175700
rosa_samantha Rh2AG228600 Rh2BG240900 Rh2CG232000 Rh2DG236600
rosa_wichuraiana Rw2G017660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 2 cut(s) 63, 344
AciI CCGC 8 cut(s) 18, 58, 61, 70, 246, 249, 338, 344
AcoI YGGCCR 2 cut(s) 52, 349
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 13, 293
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 2 cut(s) 568, 602
AluBI AGCT 6 cut(s) 7, 29, 105, 467, 479, 549
AluI AGCT 6 cut(s) 7, 29, 105, 467, 479, 549
AlwNI CAGNNNCTG 1 cut(s) 622
AoxI GGCC 3 cut(s) 52, 215, 349
ApaI GGGCCC 1 cut(s) 219
ApeKI GCWGC 4 cut(s) 4, 188, 230, 243
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 215, 216
BaeGI GKGCMC 2 cut(s) 219, 276
BalI TGGCCA 1 cut(s) 351
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 219
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 4 cut(s) 16, 200, 217, 255
BccI CCATC 2 cut(s) 81, 341
BceAI ACGGC 1 cut(s) 67
BciVI GTATCC 2 cut(s) 400, 524
BfuAI ACCTGC 1 cut(s) 607
BfuI GTATCC 2 cut(s) 400, 524
BisI GCNGC 8 cut(s) 5, 18, 61, 189, 231, 244, 247, 250
BlsI GCNGC 8 cut(s) 6, 19, 62, 190, 232, 245, 248, 251
BmgT120I GGNCC 2 cut(s) 215, 216
BmiI GGNNCC 2 cut(s) 217, 439
BmsI GCATC 2 cut(s) 319, 470
Bpu10I CCTNAGC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 49
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 271, 352
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 115
BseSI GKGCMC 2 cut(s) 219, 276
BseXI GCAGC 4 cut(s) 16, 200, 217, 255
Bsh1236I CGCG 2 cut(s) 63, 344
BshFI GGCC 3 cut(s) 54, 217, 351
BshNI GGYRCC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 50
BsnI GGCC 3 cut(s) 54, 217, 351
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 219, 276
Bsp1407I TGTACA 2 cut(s) 11, 291
BspACI CCGC 8 cut(s) 18, 58, 61, 70, 246, 249, 338, 344
BspANI GGCC 3 cut(s) 54, 217, 351
BspCNI CTCAG 1 cut(s) 114
BspFNI CGCG 2 cut(s) 63, 344
BspLI GGNNCC 2 cut(s) 217, 439
BspMI ACCTGC 1 cut(s) 607
BspQI GCTCTTC 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 20
BsrGI TGTACA 2 cut(s) 11, 291
BssECI CCNNGG 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 431, 538
Bst6I CTCTTC 1 cut(s) 231
BstAUI TGTACA 2 cut(s) 11, 291
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 2 cut(s) 271, 352
BstFNI CGCG 2 cut(s) 63, 344
BstHHI GCGC 1 cut(s) 243
BstMWI GCNNNNNNNGC 6 cut(s) 26, 60, 69, 188, 249, 446
BstSLI GKGCMC 2 cut(s) 219, 276
BstUI CGCG 2 cut(s) 63, 344
BstV1I GCAGC 4 cut(s) 16, 200, 217, 255
BsuI GTATCC 2 cut(s) 400, 524
BsuRI GGCC 3 cut(s) 54, 217, 351
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 271, 352
BveI ACCTGC 1 cut(s) 607
CaiI CAGNNNCTG 1 cut(s) 622
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 215, 216
CseI GACGC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 12, 292
CviQI GTAC 2 cut(s) 12, 292
DdeI CTNAG 1 cut(s) 101
EaeI YGGCCR 2 cut(s) 52, 349
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 219
FaiI YATR 3 cut(s) 228, 412, 427
Fnu4HI GCNGC 8 cut(s) 5, 18, 61, 189, 231, 244, 247, 250
FokI GGATG 2 cut(s) 278, 359
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 8 cut(s) 5, 18, 61, 189, 231, 244, 247, 250
GlaI GCGC 1 cut(s) 242
GluI GCNGC 8 cut(s) 5, 18, 61, 189, 231, 244, 247, 250
HaeIII GGCC 3 cut(s) 54, 217, 351
HapII CCGG 1 cut(s) 157
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 243
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 3 cut(s) 86, 286, 485
HpaII CCGG 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 3 cut(s) 199, 561, 631
Hpy188III TCNNGA 3 cut(s) 168, 502, 568
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 524
HpyCH4III ACNGT 2 cut(s) 431, 538
HpyCH4V TGCA 2 cut(s) 4, 332
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 60, 69, 188, 249, 446
HpyF3I CTNAG 1 cut(s) 101
HspAI GCGC 1 cut(s) 241
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 25, 476
LpnPI CCDG 7 cut(s) 170, 291, 464, 497, 526, 602, 608
Lsp1109I GCAGC 4 cut(s) 16, 200, 217, 255
LweI GCATC 2 cut(s) 319, 470
MaeIII GTNAC 2 cut(s) 377, 589
MbiI CCGCTC 1 cut(s) 20
MboII GAAGA 5 cut(s) 212, 248, 323, 326, 338
MhlI GDGCHC 2 cut(s) 219, 276
MlsI TGGCCA 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 3 cut(s) 60, 375, 454
MnlI CCTC 6 cut(s) 50, 110, 124, 256, 392, 397
Mox20I TGGCCA 1 cut(s) 351
MscI TGGCCA 1 cut(s) 351
MseI TTAA 3 cut(s) 321, 389, 609
Msp20I TGGCCA 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 246, 467
MspI CCGG 1 cut(s) 157
MvnI CGCG 2 cut(s) 63, 344
MwoI GCNNNNNNNGC 6 cut(s) 26, 60, 69, 188, 249, 446
NlaIV GGNNCC 2 cut(s) 217, 439
PciSI GCTCTTC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 286, 485
PkrI GCNGC 8 cut(s) 6, 19, 62, 190, 232, 245, 248, 251
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 217, 439
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 2 cut(s) 215, 216
PstNI CAGNNNCTG 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 13, 293
RsaNI GTAC 2 cut(s) 12, 292
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 3 cut(s) 321, 389, 609
SatI GCNGC 8 cut(s) 5, 18, 61, 189, 231, 244, 247, 250
Sau96I GGNCC 2 cut(s) 215, 216
SchI GAGTC 1 cut(s) 80
SduI GDGCHC 2 cut(s) 219, 276
SfaNI GCATC 2 cut(s) 319, 470
SsiI CCGC 8 cut(s) 18, 58, 61, 70, 246, 249, 338, 344
TaaI ACNGT 2 cut(s) 431, 538
TaqI TCGA 4 cut(s) 107, 169, 366, 384
TaqII GACCGA 1 cut(s) 512
TatI WGTACW 2 cut(s) 11, 291
TauI GCSGC 4 cut(s) 20, 63, 249, 252
TfiI GAWTC 2 cut(s) 286, 485
Tru1I TTAA 3 cut(s) 321, 389, 609
Tru9I TTAA 3 cut(s) 321, 389, 609
TseI GCWGC 4 cut(s) 4, 188, 230, 243
TspGWI ACGGA 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.