RLG00000017919

CAAD domains of cyanobacterial aminoacyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014374
Physical Location & Seq
Reverse (-)
22954215 .. 22957128
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017919

Sequence Viewer

Length: 600 bp
ATGGAGCTCTGTACCACCACATATCAACCTCTCCCTAATCTCCTCATCACAAACCCTAACACCAACACCCTCCGCCCAAAACCCTCACTCCAATTCGCACAATCACGCCACTCTCTCAGCCTTCGCTCTCGTTTGGTTTCGATTAGAGCTACGTCTTCTGAAGAAAGTGGGGGAAATGCGTATTCTGGTGAAGAGCGTGATGGGGTGGTATCCACTTATGCTGTTCCACCGCCCGAAAAGAATGTTTATGGTCAAGCTGTGGTGGAAGAAGCACCTCAAGAGGACAGCATTGTGAATGGCAGTGATGGTCAATCGCAGATATCTGAGTTTTTGGAGAACTTTAATGTAAAGTTGGACAATGATGATGCATATGTCTATCTACTATACGGTAGTGGTGCCCTGGTTACCCTGTGGTTAGCATCAGCTATCATAGGTGCTATTGATTCTATTCCATTGTTCCCCAAGTTGATGGAAGTTGTGGGTCTTGGATACACAATCTGGTTTAGCAGTCGTTATCTGATATTCAAGAAAAACAGGGAGGAGTTTTTTGCTAAAGTCCAAGTTCTTAAGCAGCAGGTTCTTGGTTCAAATGATGACTGA

Protein Analysis

200

Amino Acids

22.02

Weight (kDa)

4.8

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 120 - 195 1.5e-25 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38100 AT4G38100
fragaria_vesca FvH4_1g19890
malus_domestica MD15G1315100.v1.1
prunus_persica Prupe.6G196600_v2.0.a1
pyrus_communis pycom15g27840
rosa_chinensis RchiOBHm_Chr2g0111711
rosa_laevigata RLG00000017919
rosa_multiflora Rmu_sc0000448.1_g000011
rosa_roxburghii Rroxscaffold_2G00132150
rosa_rugosa Rorug02G0175600 Rorug02G0175700
rosa_samantha Rh2AG228600 Rh2BG240900 Rh2CG232000 Rh2DG236600
rosa_wichuraiana Rw2G017660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 395
AciI CCGC 2 cut(s) 73, 230
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 1 cut(s) 13
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 2 cut(s) 526, 588
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 4 cut(s) 7, 149, 257, 425
AluI AGCT 4 cut(s) 7, 149, 257, 425
Alw21I GWGCWC 1 cut(s) 9
ApeKI GCWGC 1 cut(s) 571
AsuHPI GGTGA 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 583
BccI CCATC 3 cut(s) 194, 299, 463
BciT130I CCWGG 1 cut(s) 401
BciVI GTATCC 2 cut(s) 220, 482
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 565
BfuI GTATCC 2 cut(s) 220, 482
BisI GCNGC 1 cut(s) 572
BlsI GCNGC 1 cut(s) 573
Bme1390I CCNGG 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 355, 428
BpiI GAAGAC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 399
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 130, 315
BseRI GAGGAG 2 cut(s) 32, 554
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 1 cut(s) 583
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 9, 400
BspACI CCGC 2 cut(s) 73, 230
BspCNI CTCAG 2 cut(s) 129, 316
BspLI GGNNCC 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 565
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 566
BssECI CCNNGG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 1 cut(s) 186
BstAFI CTTAAG 1 cut(s) 566
BstDEI CTNAG 2 cut(s) 116, 324
BstEII GGTNACC 1 cut(s) 403
BstNI CCWGG 1 cut(s) 401
BstPI GGTNACC 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 469
BsuI GTATCC 2 cut(s) 220, 482
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 307
BveI ACCTGC 1 cut(s) 565
Csp6I GTAC 1 cut(s) 12
CviJI RGCY 5 cut(s) 7, 120, 149, 257, 425
CviKI_1 RGCY 5 cut(s) 7, 120, 149, 257, 425
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 116, 324
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 180
Eco91I GGTNACC 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 399
EcoRV GATATC 1 cut(s) 321
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 9
FaiI YATR 7 cut(s) 22, 219, 249, 370, 372, 385, 431
FauNDI CATATG 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 572
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 572
GluI GCNGC 1 cut(s) 572
HinfI GANTC 1 cut(s) 443
HphI GGTGA 1 cut(s) 200
Hpy188I TCNGA 3 cut(s) 160, 325, 519
Hpy188III TCNNGA 2 cut(s) 278, 526
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 1 cut(s) 368
HpyF3I CTNAG 2 cut(s) 116, 324
HpySE526I ACGT 1 cut(s) 152
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 171, 386, 413, 422, 484, 520, 560
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 2 cut(s) 355, 428
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 403
MboII GAAGA 4 cut(s) 147, 173, 203, 278
MhlI GDGCHC 2 cut(s) 9, 400
MluCI AATT 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 7 cut(s) 39, 53, 80, 94, 274, 285, 532
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 2 cut(s) 342, 567
MspCI CTTAAG 1 cut(s) 566
MspR9I CCNGG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 401
NdeI CATATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 397
NsiI ATGCAT 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 443
PkrI GCNGC 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 399
PspEI GGTNACC 1 cut(s) 403
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 397
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 342, 567
SatI GCNGC 1 cut(s) 572
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 2 cut(s) 9, 400
SetI ASST 9 cut(s) 9, 31, 151, 155, 259, 277, 427, 436, 579
SfaNI GCATC 2 cut(s) 355, 428
SmlI CTYRAG 2 cut(s) 276, 566
SmoI CTYRAG 2 cut(s) 276, 566
Sse9I AATT 1 cut(s) 92
SsiI CCGC 2 cut(s) 73, 230
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 140
TasI AATT 1 cut(s) 92
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 2 cut(s) 342, 567
Tru9I TTAA 2 cut(s) 342, 567
TscAI CASTG 1 cut(s) 307
TseI GCWGC 1 cut(s) 571
TspRI CASTG 1 cut(s) 307
Vha464I CTTAAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.