pycom15g29020

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010504
Physical Location & Seq
Reverse (-)
25640124 .. 25642306
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGAAGTTTGAAAGGGAGGTAAATTTGAAGTGTTTGATTGGGCAATTCCAACAAGGGTTTATGACTTTATCTGGTTCAAGCTCGATTTCATCTGTGGCTGCTCTTCTGGGTGTTGCTGGTGGCTTTGTTCTTGCTCTCAGAGGCTTGAAATTCGGCATCCTAAACACCTTACACTTGGAGTGGCTGCCACACACTGAGACTTCTTCTCCCGAGCAGCTGCCTTTGGTACCGGGCCTCCAAAATCTTGGAAATAATTGTTTTCTCAATGTTATTTTACAGGCTCTTGCTAGCTGTTCATGCTTTCAACCCTTTCTCCAGAAGGTTTTAGAGGAATATGAGTTCTCAGCAGCTGGCAATCGCGTTGATGGCTTTCCTCTTGCGATAGCTTTGGCTACTTTGTTAGAAGACCTGCGCGTAGTTGGTGGAGGAAGAGTTGTATTAAGTCCACGGAAAGTGATGCTTGCAATGGCAGATTACATTCAGAATTTCGATCTAACAAGCCAGCAGGATGCAGCAGAAGCATTTCTTCATCTTCTGTCCTCTTTAAGAGAAGAATTTTCAGATTGCTATCTTCCAAATCAATGTTCTTTGGCAGAACTCTGTGCATCTAATTGTAGGATTATTACTCCTAAGAGTACGGAGAACCAGAGTAAGCAGGCAAGGTGGCAGCAATACTACCTTGGGCCATTTGATGGAATTCTCGGTAGCATCCTCACGTGCCAAAGTTGCTCATCACAGATATCATTGAATTTCGAATCTTTTCACAGCTTGCCTCTTTCACCAGTCCTTGATGGCGGTGGCACAATTATGGTTGGATGCACCTTAGAGGATTGCCTGGAGCAGTTCACTGCTGCTGAGCAAGTTGAGAATTACCACTGTAACCATTGTTGGCACATTGCGGCAGTCAAGTATTTATTTTCTCTAGGAGTAAATGAGGTAAATCCTTTATTCCCATATTATGAAAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 map04137
Pfam Domains
Protein Families
UCH

Protein Analysis

324

Amino Acids

35.54

Weight (kDa)

4.91

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH PF00443 78 - 251 6.3e-18 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 692
AccII CGCG 2 cut(s) 360, 414
AciI CCGC 2 cut(s) 795, 899
AcsI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 748
AcvI CACGTG 1 cut(s) 717
AfaI GTAC 2 cut(s) 228, 637
AfiI CCNNNNNNNGG 1 cut(s) 692
AgsI TTSAA 6 cut(s) 11, 28, 78, 148, 305, 748
AjnI CCWGG 1 cut(s) 834
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AloI GAACNNNNNNTCC 2 cut(s) 323, 355
AluBI AGCT 6 cut(s) 81, 217, 291, 350, 386, 768
AluI AGCT 6 cut(s) 81, 217, 291, 350, 386, 768
Alw26I GTCTC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 2 cut(s) 232, 683
ApeKI GCWGC 8 cut(s) 98, 184, 214, 217, 347, 512, 667, 851
ApoI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 748
Asp700I GAANNNNTTC 2 cut(s) 522, 759
Asp718I GGTACC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 232, 683
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 771
AsuII TTCGAA 1 cut(s) 753
AsuNHI GCTAGC 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 209
BanI GGYRCC 1 cut(s) 226
BbrPI CACGTG 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 8 cut(s) 85, 171, 204, 226, 359, 524, 679, 838
BccI CCATC 3 cut(s) 359, 686, 785
BciT130I CCWGG 1 cut(s) 836
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 2 cut(s) 288, 923
BfuAI ACCTGC 1 cut(s) 417
BisI GCNGC 9 cut(s) 99, 185, 215, 218, 348, 513, 668, 852, 900
BlpI GCTNAGC 1 cut(s) 855
BlsI GCNGC 9 cut(s) 100, 186, 216, 219, 349, 514, 669, 853, 901
Bme1390I CCNGG 2 cut(s) 231, 836
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 232, 683
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 231, 836
BmsI GCATC 6 cut(s) 165, 447, 499, 614, 717, 806
BmtI GCTAGC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 411
BpmI CTGGAG 2 cut(s) 299, 857
Bpu1102I GCTNAGC 1 cut(s) 855
Bpu14I TTCGAA 1 cut(s) 753
BpuMI CCSGG 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 446, 679
BsaXI ACNNNNNCTCC 8 cut(s) 190, 219, 220, 249, 297, 327, 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 692
Bse1I ACTGG 1 cut(s) 782
Bse3DI GCAATG 2 cut(s) 471, 894
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 836
BseDI CCNNGG 2 cut(s) 446, 679
BseGI GGATG 4 cut(s) 156, 514, 708, 821
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 1 cut(s) 692
BseMI GCAATG 2 cut(s) 471, 894
BseMII CTCAG 4 cut(s) 151, 186, 357, 846
BseNI ACTGG 1 cut(s) 782
BseXI GCAGC 8 cut(s) 85, 171, 204, 226, 359, 524, 679, 838
Bsh1236I CGCG 2 cut(s) 360, 414
BshFI GGCC 2 cut(s) 234, 685
BshNI GGYRCC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 692
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 2 cut(s) 234, 685
BsoBI CYCGRG 1 cut(s) 209
Bsp119I TTCGAA 1 cut(s) 753
Bsp143I GATC 1 cut(s) 490
Bsp1720I GCTNAGC 1 cut(s) 855
BspACI CCGC 2 cut(s) 795, 899
BspANI GGCC 2 cut(s) 234, 685
BspCNI CTCAG 4 cut(s) 150, 187, 356, 847
BspFNI CGCG 2 cut(s) 360, 414
BspLI GGNNCC 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 417
BspOI GCTAGC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 753
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 471, 894
BsrI ACTGG 1 cut(s) 782
BssECI CCNNGG 2 cut(s) 446, 679
BssMI GATC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 836
Bst4CI ACNGT 1 cut(s) 878
Bst6I CTCTTC 2 cut(s) 108, 424
BstBAI YACGTR 1 cut(s) 717
BstBI TTCGAA 1 cut(s) 753
BstC8I GCNNGC 6 cut(s) 289, 352, 462, 503, 657, 770
BstDEI CTNAG 6 cut(s) 137, 195, 343, 630, 823, 855
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 4 cut(s) 156, 514, 708, 821
BstFNI CGCG 2 cut(s) 360, 414
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 4 cut(s) 297, 366, 518, 726
BstNI CCWGG 1 cut(s) 836
BstSCI CCNGG 2 cut(s) 229, 834
BstUI CGCG 2 cut(s) 360, 414
BstV1I GCAGC 8 cut(s) 85, 171, 204, 226, 359, 524, 679, 838
BstV2I GAAGAC 1 cut(s) 411
BstXI CCANNNNNNTGG 1 cut(s) 245
BsuRI GGCC 2 cut(s) 234, 685
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 4 cut(s) 156, 514, 708, 821
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 3 cut(s) 192, 846, 874
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 6 cut(s) 289, 352, 462, 503, 657, 770
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 2 cut(s) 232, 683
Csp6I GTAC 2 cut(s) 227, 636
CviAII CATG 1 cut(s) 297
CviQI GTAC 2 cut(s) 227, 636
DdeI CTNAG 6 cut(s) 137, 195, 343, 630, 823, 855
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 108, 424
EarI CTCTTC 2 cut(s) 108, 424
Eco130I CCWWGG 1 cut(s) 679
Eco32I GATATC 1 cut(s) 741
Eco72I CACGTG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 1 cut(s) 834
EcoRV GATATC 1 cut(s) 741
EcoT14I CCWWGG 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 679
FaeI CATG 1 cut(s) 300
FaiI YATR 7 cut(s) 62, 298, 336, 809, 955, 960, 968
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 510, 542
FatI CATG 1 cut(s) 296
Fnu4HI GCNGC 9 cut(s) 99, 185, 215, 218, 348, 513, 668, 852, 900
FokI GGATG 4 cut(s) 143, 521, 695, 828
Fsp4HI GCNGC 9 cut(s) 99, 185, 215, 218, 348, 513, 668, 852, 900
FspBI CTAG 2 cut(s) 288, 923
GlaI GCGC 1 cut(s) 413
GluI GCNGC 9 cut(s) 99, 185, 215, 218, 348, 513, 668, 852, 900
GsuI CTGGAG 2 cut(s) 299, 857
HaeIII GGCC 2 cut(s) 234, 685
HapII CCGG 1 cut(s) 230
HhaI GCGC 1 cut(s) 414
Hin1II CATG 1 cut(s) 300
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HinfI GANTC 1 cut(s) 755
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 2 cut(s) 446, 846
Hpy188I TCNGA 3 cut(s) 140, 483, 562
Hpy188III TCNNGA 2 cut(s) 209, 316
Hpy8I GTNNAC 2 cut(s) 446, 846
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 878
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 4 cut(s) 464, 512, 605, 819
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 366, 518, 726
HpyF3I CTNAG 6 cut(s) 137, 195, 343, 630, 823, 855
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 1 cut(s) 300
HspAI GCGC 1 cut(s) 412
KpnI GGTACC 1 cut(s) 230
Kzo9I GATC 1 cut(s) 490
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 1 cut(s) 838
Lsp1109I GCAGC 8 cut(s) 85, 171, 204, 226, 359, 524, 679, 838
LweI GCATC 6 cut(s) 165, 447, 499, 614, 717, 806
MaeI CTAG 2 cut(s) 288, 923
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 878
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 8 cut(s) 95, 195, 416, 441, 518, 524, 563, 563
MmeI TCCRAC 2 cut(s) 73, 793
MroXI GAANNNNTTC 2 cut(s) 522, 759
MseI TTAA 2 cut(s) 440, 545
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 2 cut(s) 217, 350
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 231, 836
MvaI CCWGG 1 cut(s) 836
MvnI CGCG 2 cut(s) 360, 414
MwoI GCNNNNNNNGC 4 cut(s) 297, 366, 518, 726
NciI CCSGG 1 cut(s) 231
NdeII GATC 1 cut(s) 490
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 1 cut(s) 228
NspV TTCGAA 1 cut(s) 753
PciSI GCTCTTC 1 cut(s) 108
PdmI GAANNNNTTC 2 cut(s) 522, 759
PfeI GAWTC 1 cut(s) 755
PflMI CCANNNNNTGG 1 cut(s) 692
PkrI GCNGC 9 cut(s) 100, 186, 216, 219, 349, 514, 669, 853, 901
PmaCI CACGTG 1 cut(s) 717
PmlI CACGTG 1 cut(s) 717
Ppu21I YACGTR 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 834
PspCI CACGTG 1 cut(s) 717
PspGI CCWGG 1 cut(s) 834
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 2 cut(s) 232, 683
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 2 cut(s) 217, 350
RsaI GTAC 2 cut(s) 228, 637
RsaNI GTAC 2 cut(s) 227, 636
RseI CAYNNNNRTG 1 cut(s) 806
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 2 cut(s) 440, 545
SatI GCNGC 9 cut(s) 99, 185, 215, 218, 348, 513, 668, 852, 900
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 2 cut(s) 232, 683
ScrFI CCNGG 2 cut(s) 231, 836
SfaNI GCATC 6 cut(s) 165, 447, 499, 614, 717, 806
SfuI TTCGAA 1 cut(s) 753
SmiMI CAYNNNNRTG 1 cut(s) 806
SsiI CCGC 2 cut(s) 795, 899
SspMI CTAG 2 cut(s) 288, 923
StyD4I CCNGG 2 cut(s) 229, 834
StyI CCWWGG 1 cut(s) 679
TaaI ACNGT 1 cut(s) 878
TaiI ACGT 1 cut(s) 719
TaqI TCGA 3 cut(s) 83, 489, 753
TauI GCSGC 1 cut(s) 902
TfiI GAWTC 1 cut(s) 755
Tru1I TTAA 2 cut(s) 440, 545
Tru9I TTAA 2 cut(s) 440, 545
TscAI CASTG 3 cut(s) 199, 853, 881
TseI GCWGC 8 cut(s) 98, 184, 214, 217, 347, 512, 667, 851
TspDTI ATGAA 4 cut(s) 17, 78, 285, 518
TspGWI ACGGA 2 cut(s) 463, 653
TspRI CASTG 3 cut(s) 199, 853, 881
Van91I CCANNNNNTGG 1 cut(s) 692
XapI RAATTY 6 cut(s) 22, 149, 484, 554, 696, 748
XmnI GAANNNNTTC 2 cut(s) 522, 759
XspI CTAG 2 cut(s) 288, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.