pycom15g30290

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017762
Physical Location & Seq
Forward (+)
27675944 .. 27676590
647 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g30290.4

Sequence Viewer

Length: 345 bp
ATGGGTCAGGGCCTGAGCTGCGGAGAGCCTCGTGAGAATGGAGTCTTTGGAACTGTGGAGAATGGGGATTTGGGGCTTGTCGGAGCCATGGTGGAAGCTGACCCAAGTGTTTTGGAGCTGAGCAAAGGCCGCCGCCGGCTGTCTGCTCTGCATGTCGCCGCCGCAAATGGCCGGATCGAGGTTCTGTCTATGCTGTTGGATCGGTCTGTTATCAATCCGGATGTGGTGAATCGATACAAACAGACTCCGTTAATGTTGGCTGCGATGAATGGGAATATCACCTGTGTCCAAAAGCTTATCCAAGCAGGAGCAAATATTTTGATGTTTGATTCCCTGAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4

Protein Analysis

115

Amino Acids

12.08

Weight (kDa)

5.73

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 5 cut(s) 21, 130, 133, 159, 162
AclWI GGATC 2 cut(s) 182, 207
AcoI YGGCCR 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 4 cut(s) 18, 98, 118, 295
AluI AGCT 4 cut(s) 18, 98, 118, 295
AlwI GGATC 2 cut(s) 182, 207
AlwNI CAGNNNCTG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 3 cut(s) 10, 127, 169
ApeKI GCWGC 2 cut(s) 18, 260
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 238, 271
BauI CACGAG 1 cut(s) 30
BbvI GCAGC 2 cut(s) 5, 247
BisI GCNGC 6 cut(s) 19, 130, 133, 159, 162, 261
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 6 cut(s) 20, 131, 134, 160, 163, 262
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 232
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 135
BseAI TCCGGA 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 2 cut(s) 5, 110
BseXI GCAGC 2 cut(s) 5, 247
BshFI GGCC 3 cut(s) 12, 129, 171
BshVI ATCGAT 1 cut(s) 232
BsiSI CCGG 3 cut(s) 136, 172, 218
BslI CCNNNNNNNGG 1 cut(s) 178
BsnI GGCC 3 cut(s) 12, 129, 171
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 2 cut(s) 174, 199
Bsp1720I GCTNAGC 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 5 cut(s) 21, 130, 133, 159, 162
BspANI GGCC 3 cut(s) 12, 129, 171
BspCNI CTCAG 2 cut(s) 6, 111
BspDI ATCGAT 1 cut(s) 232
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 2 cut(s) 182, 207
BsrFI RCCGGY 1 cut(s) 135
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 2 cut(s) 174, 199
BssSI CACGAG 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 14, 119
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 226
BstKTI GATC 2 cut(s) 177, 202
BstMBI GATC 2 cut(s) 174, 199
BstMWI GCNNNNNNNGC 2 cut(s) 18, 129
BstNSI RCATGY 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 5, 247
Bsu15I ATCGAT 1 cut(s) 232
BsuRI GGCC 3 cut(s) 12, 129, 171
BsuTUI ATCGAT 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 137
CaiI CAGNNNCTG 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 232
CviAII CATG 2 cut(s) 88, 152
DdeI CTNAG 2 cut(s) 14, 119
DpnI GATC 2 cut(s) 176, 201
DpnII GATC 2 cut(s) 174, 199
EaeI YGGCCR 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 91, 155
FaiI YATR 3 cut(s) 89, 153, 191
FatI CATG 2 cut(s) 87, 151
Fnu4HI GCNGC 6 cut(s) 19, 130, 133, 159, 162, 261
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 6 cut(s) 19, 130, 133, 159, 162, 261
GluI GCNGC 6 cut(s) 19, 130, 133, 159, 162, 261
HaeIII GGCC 3 cut(s) 12, 129, 171
HapII CCGG 3 cut(s) 136, 172, 218
Hin1II CATG 2 cut(s) 91, 155
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 4 cut(s) 42, 229, 244, 329
HpaII CCGG 3 cut(s) 136, 172, 218
HphI GGTGA 2 cut(s) 238, 271
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 2 cut(s) 32, 218
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 129
HpyF3I CTNAG 2 cut(s) 14, 119
Hsp92II CATG 2 cut(s) 91, 155
Kpn2I TCCGGA 1 cut(s) 217
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 174, 199
LmnI GCTCC 3 cut(s) 83, 115, 308
LpnPI CCDG 6 cut(s) 26, 149, 185, 231, 291, 295
Lsp1109I GCAGC 2 cut(s) 5, 247
MalI GATC 2 cut(s) 176, 201
MboI GATC 2 cut(s) 174, 199
MlyI GAGTC 2 cut(s) 51, 238
MmeI TCCRAC 2 cut(s) 61, 177
MnlI CCTC 2 cut(s) 39, 172
MroI TCCGGA 1 cut(s) 217
MroNI GCCGGC 1 cut(s) 135
MseI TTAA 1 cut(s) 251
MspI CCGG 3 cut(s) 136, 172, 218
MwoI GCNNNNNNNGC 2 cut(s) 18, 129
NaeI GCCGGC 1 cut(s) 137
NcoI CCATGG 1 cut(s) 87
NdeII GATC 2 cut(s) 174, 199
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 2 cut(s) 91, 155
NlaIV GGNNCC 1 cut(s) 85
NspI RCATGY 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 229, 329
PkrI GCNGC 6 cut(s) 20, 131, 134, 160, 163, 262
PleI GAGTC 2 cut(s) 50, 238
PpsI GAGTC 2 cut(s) 50, 238
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 13
SaqAI TTAA 1 cut(s) 251
SatI GCNGC 6 cut(s) 19, 130, 133, 159, 162, 261
Sau3AI GATC 2 cut(s) 174, 199
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 51, 238
SetI ASST 6 cut(s) 20, 100, 120, 183, 284, 297
SsiI CCGC 5 cut(s) 21, 130, 133, 159, 162
SspI AATATT 1 cut(s) 316
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 2 cut(s) 177, 232
TaqII GACCGA 1 cut(s) 192
TauI GCSGC 4 cut(s) 132, 135, 161, 164
TfiI GAWTC 2 cut(s) 229, 329
Tru1I TTAA 1 cut(s) 251
Tru9I TTAA 1 cut(s) 251
TseI GCWGC 2 cut(s) 18, 260
TspDTI ATGAA 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 237
XceI RCATGY 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.