pycom15g32910

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011778
Physical Location & Seq
Forward (+)
32222743 .. 32224568
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGGAGAGAAGCACTCCGGTGAGGAAGCCCCACACATCCACCGCGGATCTGCTCACCTGGTCCGAGACTCCGCTGGCTGATTCTCCTCTTCCATCCTCCGCCTCTCGGCCCCACCAGCCGTCGGATGGGATCAGAAAGGTGGTGTTTGGAGGGCAGGTGACGGACGAGGAGGTCGAGAGCTTGAACAAACGCAAACCTTGTTCGGCGCATAAGATGAAGGAGATCACCGGGAGTGGCATTTTTGCACCGAAAGCAGAAAATGATGCCGCGGAAGCTGATGGTGCGAACCCTACCCTAAAGCCAGGAATTCGAATGTACCAGCAAGCGGTTGCTGGAATCAGTCATATCTCGTTTGGTGACGAAGAGAGTGTTTCTCCCAAAAAGCCTACCACCATTCCAGAAGTGGCAAAGCAGCGGGAGCTAAGTGGAACCTTGGAAACGGAAGCAGAGAGAGAGGCAAGGCTGAAGAAGCAGCTCTCTGAATCTAAATTCAAGGAGCTTAGTGGCCATGACATATTTGCGCCCCCTCCTGAAATTTTACCCAGGACAACTACCGCTCCTCGTGCTTTGGCCTTGAAGGGAAGCATCGAGATAGGAGAACCTACTTCGCCCAACGGTCGCACGCCTGTTAAGGTTTCTAATCCTGGTGGGGGTCAGAGCAATATAATATCCATCGAGGAACCAGCGCCCAAGACAGCCAAGAAGATTTATGACAAGAAGTTTTCCGAACTCTCAGGAAATGATATATTCAAGGGCGATGTTCCTCCTTCGTCAGCTGAAAAACCACTGAGTTCGGCAAAACTACGGGAGATGAGCGGCAGCAACATCTTTGCGGATGGGAAGAAAGAGGCTCGAGACTACTTAGGAGGCGTACGCAAACCCCCAGGTGGAGAGAGTAGCATTGCTTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

305

Amino Acids

32.48

Weight (kDa)

8.44

Isoelectric Point (pI)

52.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 3 - 302 2e-149 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011778)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78150 AT1G78150 AT1G78150
fragaria_vesca FvH4_5g37630
malus_domestica MD08G1181800.v1.1 MD15G1367100.v1.1
prunus_persica Prupe.1G515000_v2.0.a1
pyrus_communis pycom08g15500 pycom15g32910
rosa_chinensis RchiOBHm_Chr7g0239311
rosa_laevigata RLG00000000764
rosa_multiflora Rmu_sc0007840.1_g000003
rosa_roxburghii Rroxscaffold_3G00221030
rosa_rugosa Rorug07G0327200
rosa_samantha Rh7AG480600 Rh7BG453300 Rh7CG498500 Rh7DG466600
rosa_wichuraiana Rw7G040260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 145
AasI GACNNNNNNGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 145
AccBSI CCGCTC 2 cut(s) 557, 816
AccII CGCG 2 cut(s) 44, 269
AclWI GGATC 2 cut(s) 54, 137
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 3 cut(s) 306, 488, 534
AcuI CTGAAG 1 cut(s) 485
AfaI GTAC 2 cut(s) 317, 873
AfiI CCNNNNNNNGG 6 cut(s) 105, 121, 125, 325, 650, 887
AgsI TTSAA 4 cut(s) 184, 493, 577, 751
AjnI CCWGG 5 cut(s) 56, 301, 542, 643, 883
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 6 cut(s) 180, 275, 421, 475, 499, 776
AluI AGCT 6 cut(s) 180, 275, 421, 475, 499, 776
Alw26I GTCTC 2 cut(s) 59, 849
AlwI GGATC 2 cut(s) 54, 137
Ama87I CYCGRG 1 cut(s) 852
AoxI GGCC 3 cut(s) 107, 505, 570
ApeKI GCWGC 3 cut(s) 412, 472, 819
ApoI RAATTY 3 cut(s) 306, 488, 534
AspLEI GCGC 3 cut(s) 208, 523, 688
AspS9I GGNCC 2 cut(s) 60, 108
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 5 cut(s) 31, 46, 169, 217, 368
AsuII TTCGAA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 852
AvaII GGWCC 1 cut(s) 60
BalI TGGCCA 1 cut(s) 507
BauI CACGAG 1 cut(s) 561
BbvI GCAGC 3 cut(s) 424, 484, 831
BccI CCATC 5 cut(s) 100, 119, 272, 680, 830
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 5 cut(s) 58, 303, 544, 645, 885
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 59, 849
BfoI RGCGCY 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 5 cut(s) 267, 413, 473, 817, 820
BlsI GCNGC 5 cut(s) 268, 414, 474, 818, 821
Bme1390I CCNGG 6 cut(s) 58, 229, 303, 544, 645, 885
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 852
BmgT120I GGNCC 2 cut(s) 60, 108
BmiI GGNNCC 3 cut(s) 110, 430, 681
BmrFI CCNGG 6 cut(s) 58, 229, 303, 544, 645, 885
BmsI GCATC 2 cut(s) 253, 594
BplI GAGNNNNNCTC 3 cut(s) 30, 358, 390
Bpu14I TTCGAA 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 229
BsaJI CCNNGG 5 cut(s) 42, 267, 432, 542, 883
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 541, 571
Bsc4I CCNNNNNNNGG 6 cut(s) 105, 121, 125, 325, 650, 887
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 5 cut(s) 58, 303, 544, 645, 885
BseDI CCNNGG 5 cut(s) 42, 267, 432, 542, 883
BseGI GGATG 4 cut(s) 35, 92, 130, 841
BseLI CCNNNNNNNGG 6 cut(s) 105, 121, 125, 325, 650, 887
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 2 cut(s) 747, 779
BseRI GAGGAG 3 cut(s) 75, 182, 549
BseXI GCAGC 3 cut(s) 424, 484, 831
Bsh1236I CGCG 2 cut(s) 44, 269
Bsh1285I CGRYCG 1 cut(s) 619
BshFI GGCC 3 cut(s) 109, 507, 572
BsiEI CGRYCG 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 852
BsiSI CCGG 2 cut(s) 17, 228
BsiWI CGTACG 1 cut(s) 871
BslI CCNNNNNNNGG 6 cut(s) 105, 121, 125, 325, 650, 887
BsmAI GTCTC 2 cut(s) 59, 849
BsnI GGCC 3 cut(s) 109, 507, 572
BsoBI CYCGRG 1 cut(s) 852
Bsp119I TTCGAA 1 cut(s) 310
Bsp143I GATC 3 cut(s) 46, 129, 222
BspANI GGCC 3 cut(s) 109, 507, 572
BspCNI CTCAG 2 cut(s) 746, 780
BspFNI CGCG 2 cut(s) 44, 269
BspLI GGNNCC 3 cut(s) 110, 430, 681
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 2 cut(s) 54, 137
BspT104I TTCGAA 1 cut(s) 310
BsrBI CCGCTC 2 cut(s) 557, 816
BsrDI GCAATG 1 cut(s) 900
BssECI CCNNGG 5 cut(s) 42, 267, 432, 542, 883
BssMI GATC 3 cut(s) 46, 129, 222
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 5 cut(s) 58, 303, 544, 645, 885
Bst4CI ACNGT 1 cut(s) 617
Bst6I CTCTTC 2 cut(s) 93, 357
BstBI TTCGAA 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 75, 324, 623
BstDEI CTNAG 5 cut(s) 422, 500, 733, 788, 862
BstDSI CCRYGG 2 cut(s) 42, 267
BstF5I GGATG 4 cut(s) 35, 92, 130, 841
BstFNI CGCG 2 cut(s) 44, 269
BstH2I RGCGCY 1 cut(s) 689
BstHHI GCGC 3 cut(s) 208, 523, 688
BstKTI GATC 3 cut(s) 49, 132, 225
BstMAI GTCTC 2 cut(s) 59, 849
BstMBI GATC 3 cut(s) 46, 129, 222
BstMCI CGRYCG 1 cut(s) 619
BstMWI GCNNNNNNNGC 7 cut(s) 115, 251, 272, 281, 418, 469, 563
BstNI CCWGG 5 cut(s) 58, 303, 544, 645, 885
BstSCI CCNGG 6 cut(s) 56, 227, 301, 542, 643, 883
BstUI CGCG 2 cut(s) 44, 269
BstV1I GCAGC 3 cut(s) 424, 484, 831
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 3 cut(s) 109, 507, 572
BtgI CCRYGG 2 cut(s) 42, 267
BtgZI GCGATG 1 cut(s) 771
BtsCI GGATG 4 cut(s) 35, 92, 130, 841
BtsIMutI CAGTG 1 cut(s) 785
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 75, 324, 623
CfoI GCGC 3 cut(s) 208, 523, 688
Cfr13I GGNCC 2 cut(s) 60, 108
Cfr42I CCGCGG 2 cut(s) 45, 270
CsiI ACCWGGT 1 cut(s) 56
Csp6I GTAC 2 cut(s) 316, 872
CviAII CATG 1 cut(s) 509
CviQI GTAC 2 cut(s) 316, 872
DdeI CTNAG 5 cut(s) 422, 500, 733, 788, 862
DpnI GATC 3 cut(s) 48, 131, 224
DpnII GATC 3 cut(s) 46, 129, 222
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 2 cut(s) 93, 357
EarI CTCTTC 2 cut(s) 93, 357
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 852
EcoRI GAATTC 1 cut(s) 306
EcoRII CCWGG 5 cut(s) 56, 301, 542, 643, 883
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 1 cut(s) 512
FaiI YATR 7 cut(s) 210, 345, 510, 515, 665, 711, 746
FatI CATG 1 cut(s) 508
FauI CCCGC 1 cut(s) 408
Fnu4HI GCNGC 5 cut(s) 267, 413, 473, 817, 820
FokI GGATG 4 cut(s) 22, 79, 137, 848
Fsp4HI GCNGC 5 cut(s) 267, 413, 473, 817, 820
GlaI GCGC 3 cut(s) 207, 522, 687
GluI GCNGC 5 cut(s) 267, 413, 473, 817, 820
HaeII RGCGCY 1 cut(s) 689
HaeIII GGCC 3 cut(s) 109, 507, 572
HapII CCGG 2 cut(s) 17, 228
HhaI GCGC 3 cut(s) 208, 523, 688
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 3 cut(s) 206, 521, 686
HinP1I GCGC 3 cut(s) 206, 521, 686
HinfI GANTC 4 cut(s) 67, 80, 336, 482
HpaII CCGG 2 cut(s) 17, 228
HphI GGTGA 5 cut(s) 31, 46, 169, 217, 368
Hpy188I TCNGA 6 cut(s) 64, 124, 134, 481, 657, 727
Hpy188III TCNNGA 6 cut(s) 175, 398, 530, 589, 735, 854
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 3 cut(s) 211, 571, 777
HpyCH4III ACNGT 1 cut(s) 617
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 251, 272, 281, 418, 469, 563
HpyF3I CTNAG 5 cut(s) 422, 500, 733, 788, 862
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 3 cut(s) 206, 521, 686
KspI CCGCGG 2 cut(s) 45, 270
Kzo9I GATC 3 cut(s) 46, 129, 222
LmnI GCTCC 3 cut(s) 418, 496, 562
Lsp1109I GCAGC 3 cut(s) 424, 484, 831
LweI GCATC 2 cut(s) 253, 594
MabI ACCWGGT 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 157, 356
MalI GATC 3 cut(s) 48, 131, 224
MbiI CCGCTC 2 cut(s) 557, 816
MboI GATC 3 cut(s) 46, 129, 222
MboII GAAGA 5 cut(s) 80, 374, 478, 715, 853
MflI RGATCY 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 3 cut(s) 306, 488, 534
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 102
Mox20I TGGCCA 1 cut(s) 507
MscI TGGCCA 1 cut(s) 507
MseI TTAA 2 cut(s) 630, 913
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 507
MspA1I CMGCKG 5 cut(s) 44, 73, 269, 415, 776
MspI CCGG 2 cut(s) 17, 228
MspR9I CCNGG 6 cut(s) 58, 229, 303, 544, 645, 885
MvaI CCWGG 5 cut(s) 58, 303, 544, 645, 885
MvnI CGCG 2 cut(s) 44, 269
MwoI GCNNNNNNNGC 7 cut(s) 115, 251, 272, 281, 418, 469, 563
NciI CCSGG 1 cut(s) 229
NdeII GATC 3 cut(s) 46, 129, 222
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 3 cut(s) 110, 430, 681
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 157, 356
NspV TTCGAA 1 cut(s) 310
OliI CACNNNNGTG 1 cut(s) 17
PaeR7I CTCGAG 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 3 cut(s) 80, 336, 482
Pfl23II CGTACG 1 cut(s) 871
PkrI GCNGC 5 cut(s) 268, 414, 474, 818, 821
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp6I CCWGG 5 cut(s) 56, 301, 542, 643, 883
PspGI CCWGG 5 cut(s) 56, 301, 542, 643, 883
PspLI CGTACG 1 cut(s) 871
PspN4I GGNNCC 3 cut(s) 110, 430, 681
PspPI GGNCC 2 cut(s) 60, 108
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 2 cut(s) 317, 873
RsaNI GTAC 2 cut(s) 316, 872
RseI CAYNNNNRTG 1 cut(s) 17
SacII CCGCGG 2 cut(s) 45, 270
SaqAI TTAA 2 cut(s) 630, 913
SatI GCNGC 5 cut(s) 267, 413, 473, 817, 820
Sau3AI GATC 3 cut(s) 46, 129, 222
Sau96I GGNCC 2 cut(s) 60, 108
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 6 cut(s) 58, 229, 303, 544, 645, 885
SexAI ACCWGGT 1 cut(s) 56
SfaNI GCATC 2 cut(s) 253, 594
Sfr274I CTCGAG 1 cut(s) 852
Sfr303I CCGCGG 2 cut(s) 45, 270
SfuI TTCGAA 1 cut(s) 310
SgrBI CCGCGG 2 cut(s) 45, 270
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 852
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 852
SmoI CTYRAG 1 cut(s) 852
Sse9I AATT 3 cut(s) 306, 488, 534
StyD4I CCNGG 6 cut(s) 56, 227, 301, 542, 643, 883
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 617
TaqI TCGA 5 cut(s) 174, 310, 588, 675, 853
TasI AATT 3 cut(s) 306, 488, 534
TauI GCSGC 2 cut(s) 269, 819
TfiI GAWTC 3 cut(s) 80, 336, 482
Tru1I TTAA 2 cut(s) 630, 913
Tru9I TTAA 2 cut(s) 630, 913
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 2 cut(s) 157, 356
TseI GCWGC 3 cut(s) 412, 472, 819
Tsp45I GTSAC 2 cut(s) 157, 356
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 2 cut(s) 176, 455
TspRI CASTG 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 306, 488, 534
XcmI CCANNNNNNNNNTGG 2 cut(s) 122, 400
XhoI CTCGAG 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.