Rroxscaffold_3G00221030

Protein of unknown function (DUF4057)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011778
Physical Location & Seq
Forward (+)
3678940 .. 3681593
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221030.1

Sequence Viewer

Length: 927 bp
ATGAGCACTCCGGTGAGGAAGCCCCACACATCCACCGCGGATCTGCTCACCTGGTCCGAGACTCCGATTGCCGACTCTCCGGCGCTCTCCTCGTCGGCCGCTCGCTCCGCTTCCCGGCCTCACCAGCCGTCGGATGGGATCAAAAAAGTGGTGTTTGGAGGTCAGGTGACGGATGAGGAGGTCGAGAGCTTAAACAAACGAAAACCTTGTTCAGGGTACAAGATGAAGGAGATGACTGGGAGTGGCATTTTTACTGGGGGAGCAGACGATGAAACATTAGAACCTGGTAGTGCTAATCCTACCCCAAATAGCAAAAGCGGAATCCGACTGTACCAGCAAGCGGTTGCTGGAATCAGTCATATCTCATTTGGTGAGGCAGAGGGTGTTGCTCCCAAAAAGCCTACTACAATTGCTGAAGTTGCAAAGCTGCGCGAGCTGAGTGGAACCTTGGAAACTGAGGCAGAGGCAGAGGCAAGGCTGAAGAAGCAGCTTTCAGACTCTAAATTTAAGGAGCTCAGTGGGCATGACATATTTGCACCCCCTCCTGAAATTTTACCTAGGGAAACTACTGCTCCACGAGCGCTGGCTTTGAAAGGAAGCATTCAAATAGGGGAACCTACTTCACCAGTGCACGAGCCTGTTAAGGTGTCTAAACCTGCTGGAGGTCAGAACAATGTTATACCAGGTGAGGACCCTGTGTCCAAGACGGCGAAGAAGATTTATGACAAGAAATTTTCCGAGCTGTCAGGAAATGACATATTCAAGGGCGATGTTCCTCCGTCCTCTGCTGAGAAACCACTGAGTGTGGCAAAATTGCGGGAGATGAGTGGCAGCAACATCTTTGCCGACGGGAAGGCAGAGGCTCGAGAATATTTGGGTGGCGTACGCAAACCCCCTGGCGGCGAGAGCAGCATTGCTTTGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4057

Protein Analysis

308

Amino Acids

32.49

Weight (kDa)

6.99

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 2 - 306 2.7e-146 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011778)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78150 AT1G78150 AT1G78150
fragaria_vesca FvH4_5g37630
malus_domestica MD08G1181800.v1.1 MD15G1367100.v1.1
prunus_persica Prupe.1G515000_v2.0.a1
pyrus_communis pycom08g15500 pycom15g32910
rosa_chinensis RchiOBHm_Chr7g0239311
rosa_laevigata RLG00000000764
rosa_multiflora Rmu_sc0007840.1_g000003
rosa_roxburghii Rroxscaffold_3G00221030
rosa_rugosa Rorug07G0327200
rosa_samantha Rh7AG480600 Rh7BG453300 Rh7CG498500 Rh7DG466600
rosa_wichuraiana Rw7G040260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 664
AccBSI CCGCTC 1 cut(s) 101
AccII CGCG 2 cut(s) 38, 432
AciI CCGC 8 cut(s) 36, 38, 99, 108, 318, 341, 817, 900
AclWI GGATC 2 cut(s) 48, 146
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 3 cut(s) 503, 549, 731
AcuI CTGAAG 2 cut(s) 435, 500
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 3 cut(s) 218, 332, 885
AfeI AGCGCT 1 cut(s) 582
AfiI CCNNNNNNNGG 7 cut(s) 114, 130, 134, 212, 340, 662, 899
AgsI TTSAA 3 cut(s) 592, 605, 763
AhdI GACNNNNNGTC 1 cut(s) 697
AjnI CCWGG 4 cut(s) 50, 283, 682, 895
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 6 cut(s) 189, 427, 436, 490, 514, 742
AluI AGCT 6 cut(s) 189, 427, 436, 490, 514, 742
Alw21I GWGCWC 3 cut(s) 8, 516, 633
Alw26I GTCTC 1 cut(s) 53
Alw44I GTGCAC 1 cut(s) 629
AlwI GGATC 2 cut(s) 48, 146
Ama87I CYCGRG 1 cut(s) 864
Aor51HI AGCGCT 1 cut(s) 582
AoxI GGCC 2 cut(s) 96, 116
ApaLI GTGCAC 1 cut(s) 629
ApeKI GCWGC 4 cut(s) 427, 487, 831, 909
ApoI RAATTY 3 cut(s) 503, 549, 731
AspA2I CCTAGG 1 cut(s) 557
AspLEI GCGC 3 cut(s) 85, 432, 583
AspS9I GGNCC 2 cut(s) 54, 691
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 7 cut(s) 25, 40, 113, 178, 383, 615, 698
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 2 cut(s) 54, 691
AvrII CCTAGG 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 633
BanII GRGCYC 1 cut(s) 516
BauI CACGAG 2 cut(s) 576, 632
Bbv12I GWGCWC 3 cut(s) 8, 516, 633
BbvI GCAGC 4 cut(s) 414, 499, 843, 921
BccI CCATC 1 cut(s) 128
BceAI ACGGC 2 cut(s) 112, 723
BciT130I CCWGG 4 cut(s) 52, 285, 684, 897
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 558
BfoI RGCGCY 2 cut(s) 86, 584
BfuAI ACCTGC 1 cut(s) 664
BisI GCNGC 6 cut(s) 99, 428, 488, 832, 901, 910
BlnI CCTAGG 1 cut(s) 557
BlsI GCNGC 6 cut(s) 100, 429, 489, 833, 902, 911
Bme1390I CCNGG 5 cut(s) 52, 115, 285, 684, 897
Bme18I GGWCC 2 cut(s) 54, 691
BmeRI GACNNNNNGTC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 2 cut(s) 54, 691
BmiI GGNNCC 3 cut(s) 445, 615, 693
BmrFI CCNGG 5 cut(s) 52, 115, 285, 684, 897
BmrI ACTGGG 2 cut(s) 246, 264
BmuI ACTGGG 2 cut(s) 246, 264
BpmI CTGGAG 1 cut(s) 681
BpuMI CCSGG 1 cut(s) 115
BsaJI CCNNGG 4 cut(s) 36, 447, 557, 895
BsaWI WCCGGW 1 cut(s) 10
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 556, 586
Bsc4I CCNNNNNNNGG 7 cut(s) 114, 130, 134, 212, 340, 662, 899
Bse1I ACTGG 3 cut(s) 241, 259, 626
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 4 cut(s) 52, 285, 684, 897
BseDI CCNNGG 4 cut(s) 36, 447, 557, 895
BseGI GGATG 3 cut(s) 29, 139, 178
BseLI CCNNNNNNNGG 7 cut(s) 114, 130, 134, 212, 340, 662, 899
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 5 cut(s) 428, 447, 529, 780, 791
BseNI ACTGG 3 cut(s) 241, 259, 626
BseRI GAGGAG 2 cut(s) 79, 191
BseSI GKGCMC 1 cut(s) 633
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 4 cut(s) 414, 499, 843, 921
Bsh1236I CGCG 2 cut(s) 38, 432
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 98, 118
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 3 cut(s) 8, 516, 633
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 3 cut(s) 11, 80, 115
BsiWI CGTACG 1 cut(s) 883
BslI CCNNNNNNNGG 7 cut(s) 114, 130, 134, 212, 340, 662, 899
BsmAI GTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 600
BsnI GGCC 2 cut(s) 98, 118
BsoBI CYCGRG 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 8, 516, 633
Bsp143I GATC 2 cut(s) 40, 138
BspACI CCGC 8 cut(s) 36, 38, 99, 108, 318, 341, 817, 900
BspANI GGCC 2 cut(s) 98, 118
BspCNI CTCAG 5 cut(s) 429, 448, 528, 781, 792
BspFNI CGCG 2 cut(s) 38, 432
BspLI GGNNCC 3 cut(s) 445, 615, 693
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 2 cut(s) 48, 146
BsrBI CCGCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 3 cut(s) 241, 259, 626
BssECI CCNNGG 4 cut(s) 36, 447, 557, 895
BssMI GATC 2 cut(s) 40, 138
BssSI CACGAG 2 cut(s) 576, 632
BssT1I CCWWGG 2 cut(s) 447, 557
Bst2BI CACGAG 2 cut(s) 576, 632
Bst2UI CCWGG 4 cut(s) 52, 285, 684, 897
Bst4CI ACNGT 1 cut(s) 330
BstC8I GCNNGC 4 cut(s) 103, 339, 434, 585
BstDEI CTNAG 5 cut(s) 437, 456, 515, 789, 800
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 2 cut(s) 210, 660
BstF5I GGATG 3 cut(s) 29, 139, 178
BstFNI CGCG 2 cut(s) 38, 432
BstH2I RGCGCY 2 cut(s) 86, 584
BstHHI GCGC 3 cut(s) 85, 432, 583
BstKTI GATC 2 cut(s) 43, 141
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 40, 138
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 9 cut(s) 107, 124, 419, 433, 484, 520, 578, 906, 909
BstNI CCWGG 4 cut(s) 52, 285, 684, 897
BstSCI CCNGG 5 cut(s) 50, 113, 283, 682, 895
BstSLI GKGCMC 1 cut(s) 633
BstUI CGCG 2 cut(s) 38, 432
BstV1I GCAGC 4 cut(s) 414, 499, 843, 921
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 2 cut(s) 98, 118
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 3 cut(s) 29, 139, 178
BtsIMutI CAGTG 3 cut(s) 523, 633, 797
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 4 cut(s) 103, 339, 434, 585
CfoI GCGC 3 cut(s) 85, 432, 583
Cfr13I GGNCC 2 cut(s) 54, 691
Cfr42I CCGCGG 1 cut(s) 39
CsiI ACCWGGT 3 cut(s) 50, 283, 682
Csp6I GTAC 3 cut(s) 217, 331, 884
CviAII CATG 1 cut(s) 524
CviQI GTAC 3 cut(s) 217, 331, 884
DdeI CTNAG 5 cut(s) 437, 456, 515, 789, 800
DpnI GATC 2 cut(s) 42, 140
DpnII GATC 2 cut(s) 40, 138
DraIII CACNNNGTG 1 cut(s) 803
DriI GACNNNNNGTC 1 cut(s) 697
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1105I GACNNNNNGTC 1 cut(s) 697
Ecl136II GAGCTC 1 cut(s) 514
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 447, 557
Eco24I GRGCYC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 54, 691
Eco47III AGCGCT 1 cut(s) 582
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 2 cut(s) 435, 500
Eco88I CYCGRG 1 cut(s) 864
EcoICRI GAGCTC 1 cut(s) 514
EcoNI CCTNNNNNAGG 2 cut(s) 210, 660
EcoO109I RGGNCCY 1 cut(s) 691
EcoRII CCWGG 4 cut(s) 50, 283, 682, 895
EcoT14I CCWWGG 2 cut(s) 447, 557
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 2 cut(s) 447, 557
FaeI CATG 1 cut(s) 527
FaiI YATR 7 cut(s) 360, 525, 530, 680, 723, 758, 925
FatI CATG 1 cut(s) 523
FauI CCCGC 1 cut(s) 810
Fnu4HI GCNGC 6 cut(s) 99, 428, 488, 832, 901, 910
FokI GGATG 3 cut(s) 16, 146, 185
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 6 cut(s) 99, 428, 488, 832, 901, 910
FspBI CTAG 1 cut(s) 558
GlaI GCGC 3 cut(s) 84, 431, 582
GluI GCNGC 6 cut(s) 99, 428, 488, 832, 901, 910
GsuI CTGGAG 1 cut(s) 681
HaeII RGCGCY 2 cut(s) 86, 584
HaeIII GGCC 2 cut(s) 98, 118
HapII CCGG 3 cut(s) 11, 80, 115
HhaI GCGC 3 cut(s) 85, 432, 583
Hin1II CATG 1 cut(s) 527
Hin6I GCGC 3 cut(s) 83, 430, 581
HinP1I GCGC 3 cut(s) 83, 430, 581
HinfI GANTC 5 cut(s) 61, 74, 321, 351, 497
HpaII CCGG 3 cut(s) 11, 80, 115
HphI GGTGA 7 cut(s) 25, 40, 113, 178, 383, 615, 698
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 7 cut(s) 58, 66, 133, 326, 496, 669, 739
Hpy188III TCNNGA 4 cut(s) 184, 545, 747, 866
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 3 cut(s) 97, 133, 851
HpyAV CCTTC 2 cut(s) 220, 847
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4V TGCA 3 cut(s) 422, 536, 631
HpyF10VI GCNNNNNNNGC 9 cut(s) 107, 124, 419, 433, 484, 520, 578, 906, 909
HpyF3I CTNAG 5 cut(s) 437, 456, 515, 789, 800
Hsp92II CATG 1 cut(s) 527
HspAI GCGC 3 cut(s) 83, 430, 581
KspI CCGCGG 1 cut(s) 39
Kzo9I GATC 2 cut(s) 40, 138
LmnI GCTCC 5 cut(s) 110, 260, 394, 511, 577
Lsp1109I GCAGC 4 cut(s) 414, 499, 843, 921
MabI ACCWGGT 3 cut(s) 50, 283, 682
MaeI CTAG 1 cut(s) 558
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 42, 140
MbiI CCGCTC 1 cut(s) 101
MboI GATC 2 cut(s) 40, 138
MboII GAAGA 3 cut(s) 493, 724, 727
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 3 cut(s) 8, 516, 633
MluCI AATT 5 cut(s) 408, 503, 549, 731, 812
MlyI GAGTC 3 cut(s) 55, 68, 491
MmeI TCCRAC 2 cut(s) 111, 349
MseI TTAA 3 cut(s) 191, 507, 642
MslI CAYNNNNRTG 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 3 cut(s) 11, 80, 115
MspR9I CCNGG 5 cut(s) 52, 115, 285, 684, 897
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 600
MvaI CCWGG 4 cut(s) 52, 285, 684, 897
MvnI CGCG 2 cut(s) 38, 432
MwoI GCNNNNNNNGC 9 cut(s) 107, 124, 419, 433, 484, 520, 578, 906, 909
NciI CCSGG 1 cut(s) 115
NdeII GATC 2 cut(s) 40, 138
NlaIII CATG 1 cut(s) 527
NlaIV GGNNCC 3 cut(s) 445, 615, 693
NmuCI GTSAC 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 864
PctI GAATGC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 321, 351
Pfl23II CGTACG 1 cut(s) 883
PkrI GCNGC 6 cut(s) 100, 429, 489, 833, 902, 911
PleI GAGTC 3 cut(s) 55, 68, 491
PpsI GAGTC 3 cut(s) 55, 68, 491
PpuMI RGGWCCY 1 cut(s) 691
Psp124BI GAGCTC 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 4 cut(s) 50, 283, 682, 895
PspGI CCWGG 4 cut(s) 50, 283, 682, 895
PspLI CGTACG 1 cut(s) 883
PspN4I GGNNCC 3 cut(s) 445, 615, 693
PspPI GGNCC 2 cut(s) 54, 691
PspPPI RGGWCCY 1 cut(s) 691
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 3 cut(s) 218, 332, 885
RsaNI GTAC 3 cut(s) 217, 331, 884
RseI CAYNNNNRTG 1 cut(s) 11
SacI GAGCTC 1 cut(s) 516
SacII CCGCGG 1 cut(s) 39
SaqAI TTAA 3 cut(s) 191, 507, 642
SatI GCNGC 6 cut(s) 99, 428, 488, 832, 901, 910
Sau3AI GATC 2 cut(s) 40, 138
Sau96I GGNCC 2 cut(s) 54, 691
SchI GAGTC 3 cut(s) 55, 68, 491
ScrFI CCNGG 5 cut(s) 52, 115, 285, 684, 897
SduI GDGCHC 3 cut(s) 8, 516, 633
SexAI ACCWGGT 3 cut(s) 50, 283, 682
Sfr274I CTCGAG 1 cut(s) 864
Sfr303I CCGCGG 1 cut(s) 39
SgrBI CCGCGG 1 cut(s) 39
SinI GGWCC 2 cut(s) 54, 691
SlaI CTCGAG 1 cut(s) 864
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 864
SmoI CTYRAG 1 cut(s) 864
Sse9I AATT 5 cut(s) 408, 503, 549, 731, 812
SsiI CCGC 8 cut(s) 36, 38, 99, 108, 318, 341, 817, 900
SspI AATATT 1 cut(s) 872
SspMI CTAG 1 cut(s) 558
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 5 cut(s) 50, 113, 283, 682, 895
StyI CCWWGG 2 cut(s) 447, 557
TaaI ACNGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 183, 865
TasI AATT 5 cut(s) 408, 503, 549, 731, 812
TauI GCSGC 2 cut(s) 101, 903
TfiI GAWTC 2 cut(s) 321, 351
Tru1I TTAA 3 cut(s) 191, 507, 642
Tru9I TTAA 3 cut(s) 191, 507, 642
TscAI CASTG 3 cut(s) 523, 633, 804
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 4 cut(s) 427, 487, 831, 909
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 239, 285
TspGWI ACGGA 2 cut(s) 185, 768
TspRI CASTG 3 cut(s) 523, 633, 804
VneI GTGCAC 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 54, 691
XagI CCTNNNNNAGG 2 cut(s) 210, 660
XapI RAATTY 3 cut(s) 503, 549, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 864
XmaJI CCTAGG 1 cut(s) 557
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.