pycom15g33070

signal peptidase complex subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009961
Physical Location & Seq
Reverse (-)
32389177 .. 32394580
5404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 333 bp
ATGGAAAACGACGCCGTTTTCAGGTCTTCACTTGTTTGGTTAGCAGTGGTAATAGTGGTAGTTGGAATATGTACTCATTCCTTGAAGAAGATGATGTTAACGTACGTGGTTGGTGTGCTGGGAATTGCTGGGCTGCTTTTGCCTGATTGGGCTTACTTTGACCGCGACTTCTCGCGGTGGACCTCTCCGGTTTCATTCGAGGAAAGAGCCTCTGAAATTTCTCAAAGATCTGGACTAATAACAAGATTTAAAAAGTATCCTCTCAGATTGGTTGCTTATGCCACAGTTTATGGGTTTGCTCTGTACAAGTGGTGGGTGTTCATATCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPC12

Protein Analysis

111

Amino Acids

12.64

Weight (kDa)

9.41

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPC12 PF06645 7 - 63 2.5e-07 Microsomal signal peptidase 12 kDa subunit (SPC12)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 165, 175
AciI CCGC 2 cut(s) 163, 175
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 3 cut(s) 73, 104, 305
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 1 cut(s) 330
AluI AGCT 1 cut(s) 330
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 180
AvaII GGWCC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 120
BciVI GTATCC 1 cut(s) 267
BfuI GTATCC 1 cut(s) 267
BglII AGATCT 1 cut(s) 227
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 120
BseYI CCCAGC 2 cut(s) 118, 128
Bsh1236I CGCG 2 cut(s) 165, 175
BsiSI CCGG 1 cut(s) 188
BsiWI CGTACG 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 21
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 2 cut(s) 163, 175
BspCNI CTCAG 1 cut(s) 276
BspFNI CGCG 2 cut(s) 165, 175
BsrGI TGTACA 1 cut(s) 303
BssMI GATC 1 cut(s) 227
BssNI GRCGYC 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 263
BstFNI CGCG 2 cut(s) 165, 175
BstKTI GATC 1 cut(s) 230
BstMBI GATC 1 cut(s) 227
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstUI CGCG 2 cut(s) 165, 175
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuI GTATCC 1 cut(s) 267
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 180
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 72, 103, 304
CviJI RGCY 4 cut(s) 133, 152, 209, 330
CviKI_1 RGCY 4 cut(s) 133, 152, 209, 330
CviQI GTAC 3 cut(s) 72, 103, 304
DdeI CTNAG 1 cut(s) 263
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
DraI TTTAAA 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 180
FaiI YATR 4 cut(s) 70, 279, 291, 323
Fnu4HI GCNGC 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 134
GluI GCNGC 1 cut(s) 134
GsaI CCCAGC 2 cut(s) 122, 132
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 20
Hin1I GRCGYC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HpaI GTTAAC 1 cut(s) 99
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 99, 180
Hpy188I TCNGA 2 cut(s) 214, 266
Hpy188III TCNNGA 1 cut(s) 231
Hpy8I GTNNAC 2 cut(s) 99, 180
Hpy99I CGWCG 1 cut(s) 14
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 101, 105
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 2 cut(s) 101, 105
Hsp92I GRCGYC 1 cut(s) 12
KspAI GTTAAC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 227
LpnPI CCDG 6 cut(s) 7, 104, 114, 156, 201, 216
Lsp1109I GCAGC 1 cut(s) 120
MaeII ACGT 2 cut(s) 101, 105
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MboII GAAGA 3 cut(s) 18, 97, 100
MflI RGATCY 1 cut(s) 227
MluCI AATT 2 cut(s) 123, 216
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 4 cut(s) 193, 193, 220, 270
MseI TTAA 2 cut(s) 98, 249
MspI CCGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 165, 175
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeII GATC 1 cut(s) 227
Pfl23II CGTACG 1 cut(s) 102
PkrI GCNGC 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 106
PspFI CCCAGC 2 cut(s) 118, 128
PspLI CGTACG 1 cut(s) 102
PspPI GGNCC 1 cut(s) 180
PsuI RGATCY 1 cut(s) 227
RsaI GTAC 3 cut(s) 73, 104, 305
RsaNI GTAC 3 cut(s) 72, 103, 304
SaqAI TTAA 2 cut(s) 98, 249
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 1 cut(s) 180
SetI ASST 5 cut(s) 26, 104, 108, 185, 332
SinI GGWCC 1 cut(s) 180
Sse9I AATT 2 cut(s) 123, 216
SsiI CCGC 2 cut(s) 163, 175
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 104, 108
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 123, 216
TatI WGTACW 2 cut(s) 71, 303
Tru1I TTAA 2 cut(s) 98, 249
Tru9I TTAA 2 cut(s) 98, 249
TscAI CASTG 1 cut(s) 51
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 183, 310
TspRI CASTG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.