Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0003586

Physical Location & Seq

Genomic Coordinates Forward (+)

33134396 .. 33147196

Gene Structure 12801 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGCTTCGAACCCCACATTTGTACGATTCAAATGTCGCCAAGTTCCCTCTCTTTGCATCTCCCCTTTCATCTCACCCTGGCGCTTCTTGCACACTAGCTTCTCCTGTTCTTGAAATGGGATTGAAGTGGAATAAACAAGCATCGGGTAGCAATCAGCAAGGAATTGTCAGAAAGACCCGGGATGGTGCCAAGCATAGCACAGCTAATGCTTACGGTCAGCGTGCTCAAGAACACGTGCTTGAGGAAGAAGCCCCCCAACTGTCAGAATCCAGCTTATCATGGAAAATTTTCCCAGGTCAAGCTTTTCCGCTGGGTGTATCAGAAGTTGACAGTGGGATAAATTTTGCTATCTTTTCACAGCATGCAACTGCTGTCACACTTTGCTTATCACTTCCAGAGAGGGAAAGGCTTGGAGGGCTGGATGGTGGAATGATGGAGCTGCCTTTGGATCCTAATGTTAACAAGACTGGTGACATTTGGCACATGTGCATCAAGGATTTGCCCCGGAGCAATGTTCTTTATGGTTATCGTATGGATGGGCCACGAGGTTGGCATCAGGGACATCGATTTGATAGCAGCATTGTGCTTGTAGATCCTTATGCAAAGCTAGTTGAAGGCCGTCGGATGTTTGGAGACACCACGAAAAAGTTATCTAAATTTCTCGGAACTTATGATTTTGATAGCTTGCCTTTTGACTGGGGAGAAAACTACAAGCTCCCAAATATACCTGAGAAGGATCTTGTTATATATGAGATGAATGTTCGTGCATATACAGCTGATGAATCCAGTGGTTTGGATCCAGATATTCGTGGTAGCTACCTTGGTATGATCGAGAAGATTCCACACCTTCAAGAGCTTGGTATCAATGCAGTGGAATTGCTGCCTGTCTTCGAATTTGATGAGTTTGAGTTTCAGAGGCGACGAAATCCTAGAGATCACATGATAAATACCTGGGGCTACTCGACAATAAATTTCTTTGCTCCTATGAGCCGCTATGCAAGTGCTGGTGGAGGACCCATTAAAGCTTCTCAGGAATTTAAACAAATGGTTAAAGCCTTGCATGGTGCTGGGATAGAGGTCATTTTGGATGTTGTCTACAATCATACTAATGAAGCTGATGATGCAAACCCCTATACAACTTCATTTCGTGGCATAGATAATAAGGTTTACTACATGCTGGACGTGAACAACAATGGCGAATTACTGAACTACTCTGGCTGTGGGAACACGTTAAACTGTAACCATCCTGTTGTCATGGAGCTCATTCTTGACAGCTTAAGACATTGGGTTGTTGAATATCATGTAGATGGGTTTAGATTTGACCTTGCCAGTGTTCTTTGTCGGGGAACAGATGGAACTCCTCTTGACGCACCACCAGTTATTAGGGCTATTGCAAAAGATGCTATCCTATCAAGATGTAAAATCATTTCCGAACCTTGGGATTGTGGAGGCCTTTATCTTGTTGGAAGATTTCCAAATTGGGACAGGTGGGCTGAATGGAATGGGAAATATCGTGATGACCTAAGGAGATTTATAAAGGGTGACTCTGGTATGAAGGGAAGTTTTGCAACTCGTGTTTCTGGGTCTGCTGACCTTTACAAAGTGAATAAGCGCAAACCCTATCACAGTGTTAATTTTATTATTGCCCATGATGGGTTCACTCTATATGATCTTGTTTCATACAATTTCAAGCACAACGATGCTAATGGAGAAGGAGGAAATGATGGAAGTAATGATAATTTGAGCTGGAATTGTGGTCATGAAGGAGAAACTGATGATTCTACTATTAAAGCTATACGTTCCCGCCAAATGAAAAATTTTCATTTGGCATTAATGATATCCCAGGGAACCCCAATGATGCTGATGGGAGATGAATATGGGCATACTCGACATGGAAATAACAACAGTTATGGACATGATACTGCTCTTAACCATTTCCAGTGGGGACAGTTGGAGGCACGGAACAACAATCACTTCAGGTTTTTCAAGGAGGCAATAAAGTTTAGACGAGCACATCGTGTATTTGGTCGTGAAACTTTTCTTGAGAAGCATGATGTGACATGGCATGAAGACAACTGGGACAACTATGAGAGCAAATTCCTTGCATTTACGTTTCATGACAGTAGTGAAGGCGACATCTATTTGGCATTCAATGCTCATGACTGTTTTATTACAGTTCCAATACCGGCACCACCAGAAAAGAGGAGATGGTTTCGTGTGGTTGACACGAATCTGGAGTCTCCCAATGACATTGTGCCTGATGGCGTCCCGGGCATTGAAGGCAATTATAACATGGCCCCCTTCTCCTCTATTCTTCTTCAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004133 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009657 GO:0009660 GO:0009987 GO:0015980 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0019156 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0071840 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase CBM_48

Protein Analysis

777

Amino Acids

87.78

Weight (kDa)

5.92

Isoelectric Point (pI)

36.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	103 - 201	4.9e-12	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	266 - 383	1.1e-09	Alpha amylase, catalytic domain
ISOA1-3_C	PF21156	689 - 754	7.3e-12	Isoamylase 1-3, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003586)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G09020
fragaria_vesca	FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440 FvH4_5g36440
malus_domestica	MD08G1188900.v1.1 MD15G1376800.v1.1
prunus_persica	Prupe.1G523500_v2.0.a1 Prupe.1G523500_v2.0.a1
pyrus_communis	pycom08g16260 pycom15g33660
rosa_chinensis	RchiOBHm_Chr7g0238421
rosa_laevigata	RLG00000000911
rosa_multiflora	Rmu_sc0031277.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223020
rosa_rugosa	Rorug07G0312600
rosa_samantha	Rh7AG467500 Rh7BG438200 Rh7CG485500 Rh7DG454000
rosa_wichuraiana	Rw7G038820

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1535, 2289
AccB1I	GGYRCC	2 cut(s)	185, 2188
AccI	GTMKAC	1 cut(s)	1095
AciI	CCGC	3 cut(s)	308, 991, 1804
AclWI	GGATC	6 cut(s)	443, 456, 587, 744, 791, 804
AcsI	RAATTY	8 cut(s)	285, 340, 656, 893, 970, 1034, 1816, 2096
AcuI	CTGAAG	1 cut(s)	1960
AcvI	CACGTG	1 cut(s)	235
AcyI	GRCGYC	1 cut(s)	2265
AdeI	CACNNNGTG	1 cut(s)	2018
AfaI	GTAC	1 cut(s)	23
AfiI	CCNNNNNNNGG	2 cut(s)	1437, 1653
AflII	CTTAAG	1 cut(s)	1276
AflIII	ACRYGT	3 cut(s)	232, 483, 1227
AjiI	CACGTC	1 cut(s)	1183
AjnI	CCWGG	4 cut(s)	76, 292, 950, 1842
Alw21I	GWGCWC	3 cut(s)	226, 1263, 2014
Alw26I	GTCTC	2 cut(s)	627, 2244
AlwI	GGATC	6 cut(s)	443, 456, 587, 744, 791, 804
Ama87I	CYCGRG	2 cut(s)	177, 2269
AoxI	GGCC	4 cut(s)	539, 616, 1450, 2295
ApeKI	GCWGC	3 cut(s)	439, 576, 880
ApoI	RAATTY	8 cut(s)	285, 340, 656, 893, 970, 1034, 1816, 2096
ArsI	GACNNNNNNTTYG	2 cut(s)	2222, 2254
AseI	ATTAAT	1 cut(s)	1832
Asp700I	GAANNNNTTC	2 cut(s)	287, 2037
AspLEI	GCGC	2 cut(s)	83, 1614
AspS9I	GGNCC	3 cut(s)	539, 1013, 2296
AsuC2I	CCSGG	5 cut(s)	178, 179, 505, 2270, 2271
AsuHPI	GGTGA	3 cut(s)	65, 482, 1553
AsuII	TTCGAA	2 cut(s)	7, 891
AvaI	CYCGRG	2 cut(s)	177, 2269
AvaII	GGWCC	1 cut(s)	1013
AxyI	CCTNAGG	1 cut(s)	1523
BamHI	GGATCC	2 cut(s)	448, 796
BanI	GGYRCC	2 cut(s)	185, 2188
BanII	GRGCYC	1 cut(s)	1263
BauI	CACGAG	2 cut(s)	543, 1572
BbrPI	CACGTG	1 cut(s)	235
BbsI	GAAGAC	2 cut(s)	880, 2076
Bbv12I	GWGCWC	3 cut(s)	226, 1263, 2014
BbvI	GCAGC	3 cut(s)	426, 588, 867
BceAI	ACGGC	1 cut(s)	603
BciT130I	CCWGG	4 cut(s)	78, 294, 952, 1844
BcnI	CCSGG	5 cut(s)	178, 179, 505, 2270, 2271
BcoDI	GTCTC	2 cut(s)	627, 2244
BfaI	CTAG	3 cut(s)	95, 608, 930
BfoI	RGCGCY	1 cut(s)	84
BfrI	CTTAAG	1 cut(s)	1276
BisI	GCNGC	4 cut(s)	440, 577, 881, 991
BlsI	GCNGC	4 cut(s)	441, 578, 882, 992
Bme1390I	CCNGG	9 cut(s)	78, 178, 179, 294, 505, 952, 1844, 2270, 2271
Bme18I	GGWCC	1 cut(s)	1013
BmeT110I	CYCGRG	2 cut(s)	177, 2269
BmgBI	CACGTC	1 cut(s)	1183
BmgT120I	GGNCC	3 cut(s)	539, 1013, 2296
BmiI	GGNNCC	7 cut(s)	187, 450, 798, 1015, 1849, 2190, 2298
BmrFI	CCNGG	9 cut(s)	78, 178, 179, 294, 505, 952, 1844, 2270, 2271
BmrI	ACTGGG	2 cut(s)	706, 2086
BmsI	GCATC	8 cut(s)	65, 149, 498, 562, 1111, 1390, 1690, 1848
BmuI	ACTGGG	2 cut(s)	706, 2086
BpiI	GAAGAC	2 cut(s)	880, 2076
BpmI	CTGGAG	1 cut(s)	2255
Bpu14I	TTCGAA	2 cut(s)	7, 891
BpuEI	CTTGAG	3 cut(s)	210, 260, 2063
BpuMI	CCSGG	5 cut(s)	178, 179, 505, 2270, 2271
Bsa29I	ATCGAT	1 cut(s)	565
BsaAI	YACGTR	1 cut(s)	235
BsaHI	GRCGYC	1 cut(s)	2265
Bsc4I	CCNNNNNNNGG	2 cut(s)	1437, 1653
Bse118I	RCCGGY	1 cut(s)	2185
Bse1I	ACTGG	7 cut(s)	472, 701, 786, 1329, 1376, 1939, 2081
Bse21I	CCTNAGG	1 cut(s)	1523
Bse3DI	GCAATG	1 cut(s)	517
BseBI	CCWGG	4 cut(s)	78, 294, 952, 1844
BseCI	ATCGAT	1 cut(s)	565
BseGI	GGATG	6 cut(s)	187, 427, 541, 630, 1093, 1243
BseLI	CCNNNNNNNGG	2 cut(s)	1437, 1653
BseMI	GCAATG	1 cut(s)	517
BseMII	CTCAG	2 cut(s)	720, 1043
BseNI	ACTGG	7 cut(s)	472, 701, 786, 1329, 1376, 1939, 2081
BseRI	GAGGAG	3 cut(s)	1350, 2218, 2296
BseXI	GCAGC	3 cut(s)	426, 588, 867
BseYI	CCCAGC	2 cut(s)	310, 1067
BshFI	GGCC	4 cut(s)	541, 618, 1452, 2297
BshNI	GGYRCC	2 cut(s)	185, 2188
BshVI	ATCGAT	1 cut(s)	565
BsiHKAI	GWGCWC	3 cut(s)	226, 1263, 2014
BsiHKCI	CYCGRG	2 cut(s)	177, 2269
BsiSI	CCGG	4 cut(s)	178, 505, 2186, 2270
BslFI	GGGAC	5 cut(s)	573, 1496, 1959, 2093, 2252
BslI	CCNNNNNNNGG	2 cut(s)	1437, 1653
BsmAI	GTCTC	2 cut(s)	627, 2244
BsmFI	GGGAC	5 cut(s)	573, 1496, 1959, 2093, 2252
BsmI	GAATGC	1 cut(s)	2147
BsnI	GGCC	4 cut(s)	541, 618, 1452, 2297
BsoBI	CYCGRG	2 cut(s)	177, 2269
Bsp119I	TTCGAA	2 cut(s)	7, 891
Bsp1286I	GDGCHC	3 cut(s)	226, 1263, 2014
Bsp143I	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
BspACI	CCGC	3 cut(s)	308, 991, 1804
BspANI	GGCC	4 cut(s)	541, 618, 1452, 2297
BspCNI	CTCAG	2 cut(s)	721, 1042
BspDI	ATCGAT	1 cut(s)	565
BspHI	TCATGA	3 cut(s)	1759, 2116, 2158
BspLI	GGNNCC	7 cut(s)	187, 450, 798, 1015, 1849, 2190, 2298
BspPI	GGATC	6 cut(s)	443, 456, 587, 744, 791, 804
BspT104I	TTCGAA	2 cut(s)	7, 891
BspT107I	GGYRCC	2 cut(s)	185, 2188
BspTI	CTTAAG	1 cut(s)	1276
BsrDI	GCAATG	1 cut(s)	517
BsrFI	RCCGGY	1 cut(s)	2185
BsrI	ACTGG	7 cut(s)	472, 701, 786, 1329, 1376, 1939, 2081
BssAI	RCCGGY	1 cut(s)	2185
BssMI	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
BssNI	GRCGYC	1 cut(s)	2265
BssSI	CACGAG	2 cut(s)	543, 1572
BssT1I	CCWWGG	2 cut(s)	820, 1436
Bst2BI	CACGAG	2 cut(s)	543, 1572
Bst2UI	CCWGG	4 cut(s)	78, 294, 952, 1844
BstACI	GRCGYC	1 cut(s)	2265
BstAFI	CTTAAG	1 cut(s)	1276
BstAPI	GCANNNNNTGC	2 cut(s)	1400, 2153
BstBAI	YACGTR	1 cut(s)	235
BstBI	TTCGAA	2 cut(s)	7, 891
BstC8I	GCNNGC	3 cut(s)	222, 363, 686
BstDEI	CTNAG	3 cut(s)	729, 1029, 1523
BstF5I	GGATG	6 cut(s)	187, 427, 541, 630, 1093, 1243
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	2 cut(s)	83, 1614
BstKTI	GATC	7 cut(s)	451, 595, 739, 799, 831, 937, 1672
BstMAI	GTCTC	2 cut(s)	627, 2244
BstMBI	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
BstMWI	GCNNNNNNNGC	8 cut(s)	87, 415, 773, 1121, 1400, 2153, 2271, 2280
BstNI	CCWGG	4 cut(s)	78, 294, 952, 1844
BstNSI	RCATGY	3 cut(s)	365, 487, 1177
BstSCI	CCNGG	9 cut(s)	76, 176, 177, 292, 503, 950, 1842, 2268, 2269
BstV1I	GCAGC	3 cut(s)	426, 588, 867
BstV2I	GAAGAC	2 cut(s)	880, 2076
BstX2I	RGATCY	4 cut(s)	448, 592, 736, 796
BstXI	CCANNNNNNTGG	2 cut(s)	549, 793
BstYI	RGATCY	4 cut(s)	448, 592, 736, 796
Bsu15I	ATCGAT	1 cut(s)	565
Bsu36I	CCTNAGG	1 cut(s)	1523
BsuRI	GGCC	4 cut(s)	541, 618, 1452, 2297
BsuTUI	ATCGAT	1 cut(s)	565
BtrI	CACGTC	1 cut(s)	1183
BtsCI	GGATG	6 cut(s)	187, 427, 541, 630, 1093, 1243
BtsI	GCAGTG	1 cut(s)	876
BtsIMutI	CAGTG	6 cut(s)	337, 793, 876, 1336, 1633, 1946
Cac8I	GCNNGC	3 cut(s)	222, 363, 686
CciI	TCATGA	3 cut(s)	1759, 2116, 2158
CfoI	GCGC	2 cut(s)	83, 1614
Cfr10I	RCCGGY	1 cut(s)	2185
Cfr13I	GGNCC	3 cut(s)	539, 1013, 2296
Cfr9I	CCCGGG	2 cut(s)	177, 2269
ClaI	ATCGAT	1 cut(s)	565
CseI	GACGC	2 cut(s)	1376, 2254
Csp6I	GTAC	1 cut(s)	22
CviQI	GTAC	1 cut(s)	22
DdeI	CTNAG	3 cut(s)	729, 1029, 1523
DpnI	GATC	7 cut(s)	450, 594, 738, 798, 830, 936, 1671
DpnII	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
DraI	TTTAAA	1 cut(s)	1039
DraIII	CACNNNGTG	1 cut(s)	2018
Ecl136II	GAGCTC	1 cut(s)	1261
Eco130I	CCWWGG	2 cut(s)	820, 1436
Eco147I	AGGCCT	1 cut(s)	1452
Eco24I	GRGCYC	1 cut(s)	1263
Eco32I	GATATC	1 cut(s)	1839
Eco47I	GGWCC	1 cut(s)	1013
Eco53kI	GAGCTC	1 cut(s)	1261
Eco57I	CTGAAG	1 cut(s)	1960
Eco72I	CACGTG	1 cut(s)	235
Eco81I	CCTNAGG	1 cut(s)	1523
Eco88I	CYCGRG	2 cut(s)	177, 2269
EcoICRI	GAGCTC	1 cut(s)	1261
EcoO109I	RGGNCCY	1 cut(s)	1013
EcoRII	CCWGG	4 cut(s)	76, 292, 950, 1842
EcoRV	GATATC	1 cut(s)	1839
EcoT14I	CCWWGG	2 cut(s)	820, 1436
EcoT38I	GRGCYC	1 cut(s)	1263
ErhI	CCWWGG	2 cut(s)	820, 1436
FaqI	GGGAC	5 cut(s)	573, 1496, 1959, 2093, 2252
FauI	CCCGC	1 cut(s)	1811
FblI	GTMKAC	1 cut(s)	1095
Fnu4HI	GCNGC	4 cut(s)	440, 577, 881, 991
FokI	GGATG	6 cut(s)	194, 434, 548, 637, 1100, 1230
FriOI	GRGCYC	1 cut(s)	1263
Fsp4HI	GCNGC	4 cut(s)	440, 577, 881, 991
FspBI	CTAG	3 cut(s)	95, 608, 930
GlaI	GCGC	2 cut(s)	82, 1613
GluI	GCNGC	4 cut(s)	440, 577, 881, 991
GsaI	CCCAGC	2 cut(s)	314, 1071
GsuI	CTGGAG	1 cut(s)	2255
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	4 cut(s)	541, 618, 1452, 2297
HapII	CCGG	4 cut(s)	178, 505, 2186, 2270
HgaI	GACGC	2 cut(s)	1376, 2254
HhaI	GCGC	2 cut(s)	83, 1614
Hin1I	GRCGYC	1 cut(s)	2265
Hin6I	GCGC	2 cut(s)	81, 1612
HinP1I	GCGC	2 cut(s)	81, 1612
HincII	GTYRAC	3 cut(s)	328, 460, 2224
HindII	GTYRAC	3 cut(s)	328, 460, 2224
HindIII	AAGCTT	2 cut(s)	300, 1023
HinfI	GANTC	8 cut(s)	26, 266, 782, 838, 1544, 1778, 2230, 2237
HpaI	GTTAAC	1 cut(s)	460
HpaII	CCGG	4 cut(s)	178, 505, 2186, 2270
HphI	GGTGA	3 cut(s)	65, 482, 1553
Hpy166II	GTNNAC	7 cut(s)	328, 460, 1096, 1168, 1186, 1659, 2224
Hpy188I	TCNGA	7 cut(s)	170, 265, 322, 624, 665, 915, 1432
Hpy8I	GTNNAC	7 cut(s)	328, 460, 1096, 1168, 1186, 1659, 2224
Hpy99I	CGWCG	2 cut(s)	624, 924
HpyAV	CCTTC	9 cut(s)	608, 727, 857, 1549, 1706, 1757, 2123, 2273, 2311
HpyCH4IV	ACGT	5 cut(s)	234, 1182, 1229, 1798, 2111
HpyF10VI	GCNNNNNNNGC	8 cut(s)	87, 415, 773, 1121, 1400, 2153, 2271, 2280
HpyF3I	CTNAG	3 cut(s)	729, 1029, 1523
HpySE526I	ACGT	5 cut(s)	234, 1182, 1229, 1798, 2111
Hsp92I	GRCGYC	1 cut(s)	2265
HspAI	GCGC	2 cut(s)	81, 1612
KspAI	GTTAAC	1 cut(s)	460
Kzo9I	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
LmnI	GCTCC	5 cut(s)	436, 507, 720, 985, 1258
Lsp1109I	GCAGC	3 cut(s)	426, 588, 867
LweI	GCATC	8 cut(s)	65, 149, 498, 562, 1111, 1390, 1690, 1848
MaeI	CTAG	3 cut(s)	95, 608, 930
MaeII	ACGT	5 cut(s)	234, 1182, 1229, 1798, 2111
MaeIII	GTNAC	5 cut(s)	373, 470, 1238, 1541, 2056
MalI	GATC	7 cut(s)	450, 594, 738, 798, 830, 936, 1671
MboI	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
MboII	GAAGA	7 cut(s)	257, 847, 880, 1479, 2081, 2306, 2309
MflI	RGATCY	4 cut(s)	448, 592, 736, 796
MhlI	GDGCHC	3 cut(s)	226, 1263, 2014
MlyI	GAGTC	2 cut(s)	1538, 2246
MmeI	TCCRAC	3 cut(s)	602, 1444, 1932
MroXI	GAANNNNTTC	2 cut(s)	287, 2037
MslI	CAYNNNNRTG	3 cut(s)	1107, 1305, 1698
MspA1I	CMGCKG	2 cut(s)	310, 776
MspCI	CTTAAG	1 cut(s)	1276
MspI	CCGG	4 cut(s)	178, 505, 2186, 2270
MspR9I	CCNGG	9 cut(s)	78, 178, 179, 294, 505, 952, 1844, 2270, 2271
Mva1269I	GAATGC	1 cut(s)	2147
MvaI	CCWGG	4 cut(s)	78, 294, 952, 1844
MwoI	GCNNNNNNNGC	8 cut(s)	87, 415, 773, 1121, 1400, 2153, 2271, 2280
NciI	CCSGG	5 cut(s)	178, 179, 505, 2270, 2271
NdeII	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
NlaIV	GGNNCC	7 cut(s)	187, 450, 798, 1015, 1849, 2190, 2298
NmuCI	GTSAC	4 cut(s)	373, 470, 1541, 2056
NspI	RCATGY	3 cut(s)	365, 487, 1177
NspV	TTCGAA	2 cut(s)	7, 891
PaeI	GCATGC	1 cut(s)	365
PagI	TCATGA	3 cut(s)	1759, 2116, 2158
PasI	CCCWGGG	1 cut(s)	1843
PceI	AGGCCT	1 cut(s)	1452
PciI	ACATGT	1 cut(s)	483
PcsI	WCGNNNNNNNCGW	1 cut(s)	2014
PctI	GAATGC	1 cut(s)	2147
PdmI	GAANNNNTTC	2 cut(s)	287, 2037
PfeI	GAWTC	6 cut(s)	26, 266, 782, 838, 1778, 2230
PkrI	GCNGC	4 cut(s)	441, 578, 882, 992
PleI	GAGTC	2 cut(s)	1538, 2245
PmaCI	CACGTG	1 cut(s)	235
PmlI	CACGTG	1 cut(s)	235
PpsI	GAGTC	2 cut(s)	1538, 2245
Ppu21I	YACGTR	1 cut(s)	235
PpuMI	RGGWCCY	1 cut(s)	1013
PscI	ACATGT	1 cut(s)	483
PshBI	ATTAAT	1 cut(s)	1832
PsiI	TTATAA	2 cut(s)	1535, 2289
Psp124BI	GAGCTC	1 cut(s)	1263
Psp5II	RGGWCCY	1 cut(s)	1013
Psp6I	CCWGG	4 cut(s)	76, 292, 950, 1842
PspCI	CACGTG	1 cut(s)	235
PspFI	CCCAGC	2 cut(s)	310, 1067
PspGI	CCWGG	4 cut(s)	76, 292, 950, 1842
PspN4I	GGNNCC	7 cut(s)	187, 450, 798, 1015, 1849, 2190, 2298
PspPI	GGNCC	3 cut(s)	539, 1013, 2296
PspPPI	RGGWCCY	1 cut(s)	1013
PsuI	RGATCY	4 cut(s)	448, 592, 736, 796
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	1 cut(s)	23
RsaNI	GTAC	1 cut(s)	22
RseI	CAYNNNNRTG	3 cut(s)	1107, 1305, 1698
SacI	GAGCTC	1 cut(s)	1263
SatI	GCNGC	4 cut(s)	440, 577, 881, 991
Sau3AI	GATC	7 cut(s)	448, 592, 736, 796, 828, 934, 1669
Sau96I	GGNCC	3 cut(s)	539, 1013, 2296
SchI	GAGTC	2 cut(s)	1538, 2246
ScrFI	CCNGG	9 cut(s)	78, 178, 179, 294, 505, 952, 1844, 2270, 2271
SduI	GDGCHC	3 cut(s)	226, 1263, 2014
SfaNI	GCATC	8 cut(s)	65, 149, 498, 562, 1111, 1390, 1690, 1848
SfuI	TTCGAA	2 cut(s)	7, 891
SinI	GGWCC	1 cut(s)	1013
SmaI	CCCGGG	2 cut(s)	179, 2271
SmiMI	CAYNNNNRTG	3 cut(s)	1107, 1305, 1698
SmlI	CTYRAG	4 cut(s)	225, 239, 1276, 2042
SmoI	CTYRAG	4 cut(s)	225, 239, 1276, 2042
SphI	GCATGC	1 cut(s)	365
SseBI	AGGCCT	1 cut(s)	1452
SsiI	CCGC	3 cut(s)	308, 991, 1804
SspMI	CTAG	3 cut(s)	95, 608, 930
SstI	GAGCTC	1 cut(s)	1263
StuI	AGGCCT	1 cut(s)	1452
StyD4I	CCNGG	9 cut(s)	76, 176, 177, 292, 503, 950, 1842, 2268, 2269
StyI	CCWWGG	2 cut(s)	820, 1436
TaiI	ACGT	5 cut(s)	237, 1185, 1232, 1801, 2114
TaqI	TCGA	6 cut(s)	7, 565, 831, 891, 962, 1888
TauI	GCSGC	1 cut(s)	993
TfiI	GAWTC	6 cut(s)	26, 266, 782, 838, 1778, 2230
TscAI	CASTG	6 cut(s)	337, 793, 876, 1336, 1633, 1946
TseFI	GTSAC	4 cut(s)	373, 470, 1541, 2056
TseI	GCWGC	3 cut(s)	439, 576, 880
Tsp45I	GTSAC	4 cut(s)	373, 470, 1541, 2056
TspGWI	ACGGA	1 cut(s)	1975
TspMI	CCCGGG	2 cut(s)	177, 2269
TspRI	CASTG	6 cut(s)	337, 793, 876, 1336, 1633, 1946
Vha464I	CTTAAG	1 cut(s)	1276
VpaK11BI	GGWCC	1 cut(s)	1013
VspI	ATTAAT	1 cut(s)	1832
XapI	RAATTY	8 cut(s)	285, 340, 656, 893, 970, 1034, 1816, 2096
XceI	RCATGY	3 cut(s)	365, 487, 1177
XmaI	CCCGGG	2 cut(s)	177, 2269
XmiI	GTMKAC	1 cut(s)	1095
XmnI	GAANNNNTTC	2 cut(s)	287, 2037
XspI	CTAG	3 cut(s)	95, 608, 930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.