pycom15g34300

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007329
Physical Location & Seq
Forward (+)
33943284 .. 33952708
9425 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2754 bp
ATGACTAACCGAATCACCGATGCGCCTCAGGGAGAAGGCGATGCTCCTCCTCCTCCTCCCCCATCTGGTGGCCCTGGTCCACCTGAATCTCAGCCTTCTCGTGGTGGCAACACGATTTTCAAGAGTGGGCCGTTGTTTATATCATCCAAAGGAATTGGATGGACGTCCTGGAAGAAAAGATGGTTTATCTTAACCCGCACTTCACTCGTTTTCTTCAGAAGTGATCCAAGTGCTGCTCTAAAGGGGAGTGAAGTGAATTTGACCCTGGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGCAGACAAGAAACTCTTGACAGTGCTCTTTCCAGATGGCCGTGATGGACGAGCCTTCACACTTAAGGCTGAAACATTGGAGGATCTACATGAGTGGAAGACTGCACTTGAAAATGCGTTGTCACAATCCCCAAGTGGTGGCGTTCATGCTATGGGGCAAAATGGAATCTTAGGGAATGATAAGACTGAGGCAGTTGATGGTTCTGTGGATCAGTCGAAGGATAAGAAGCCAGTGAAATCTACAGTCATTGGAGTGCCTGTTCTGCTGGCCTTGGAAGATGTTGATGGAGCTCCGTCCTTCTTGGAAAAAGCCCTTAGGTTTGTGGAAGAATATGGAGTCAAAGTAGAAGGGATCTTACGACAAGCTGCAGACGTTGAAGATGTGGAGAGTCGAATAAGAGCGTATGAACAGGGGAAAACTGAGTTTTCTTGTGAGGAGGATGCACATGTTATTGCTGATTGTGTCAAGTACGTCCTCCGGGCGTTGCCATCTTCCCCGGTCCCTGCGTCTTGTTGTAACGCACTACTAGATGCCTTTCGTAAATCTGGTAATGATCGTGGTGGTAGAGTCAATGCTATGCGCACAACAATATGTGATACATTCCCGGAGCCAAATCGCCATTTATTGCAAAGAATTCTTTTGATGATGCAAAGTGTGGCTGCTCAGAAAGCTGTGAACCGGATGAGCTGCTCAGCTGTTGCAGCTTGTATGGCTCCCTTGCTTCTTCGTCCACTCTTAGCTGGGGATTGTGAAGTTGACAATGATATTGATGTGGGGGGTGATGGTTCTGTTCAACTATTGCAAGCAGCTGCTGCCGCTAATCATGCTCAAGCGATTGTTATAACTTTGTTGGAGGAGTATAATCACATATTTGGGGAAGGTGGTCCCCTGTCCCAGGAACTATACAGTGACACAGAAGATAGTGAAAGTGAGACCGAGGGGGCAACCGATGATGACGATTACTATGAAGATGATGAAACTGATGCAGAAACAGGGGATGAAGACGTGGAAATTGTTTCTGATGGAGATGGAACGTGCAGTGAGAGTGGTCAATCTGAAGACAAGGATCAGCATGATGATAAGGATGATGACGGTTTAAGACTAGGTGTGAAGTCTCCTCATGGAGGCAATGATGGCAAAGCTCACGAGAAATTGTCATCAAGCTCAATTAAAGTTTTGCAGCCTCAACATGCTGTAGAGAGCAGTAAGAATGAACTGAGCTTAAGTAAAACCAATGCGCTAGAGCAGGCTAATGAGTCATCTGTAGTAGAAGAGGTTTCTAGAGAAATAAGTTCACTGAACCAGCCACCCGTTCCATCCCGATCCATCCAAAAATCTTCAACTATATCCAATGAACCTGAGCATGGCAGGAATCGTACTGGTTGGGGACGTACCGCTGCAAAGAGGAACCTTTCCATGGAGTCCATTGACTACGGCCTTGAGGAAGAGGCTGAAATCCAGAAGCTTGAGATGACTAAATCTGAGTTAGAAAACAGAATTGCAGAAGAGGCTGAAGCGAATGCTGCTCTACAAGCAAGTCTGGAGAGACAGAAGACGGCCTTGCATGAGCGTCGCCTAGCTCTCGAGCAAGATGTGGCAAGACTACAGGAACAGTTACTGAAGGAGAAGGATTTAACAGCAGCTCTTGAAGCAGGACTAAAAGGGAAGGGGGGACGTGTGCCCAATTTAGCCGCAGTTGATGAAAAAACAAGGGCAGATCTTACTGAAATAGCTCAGGCAGAGGAGAATGTTGCCAACTTGAACAAGAAGGTTGATGATCTTAGCATGCAGCTTAATCAACAGCGTGATCAAAACCATGGCCCTATGGCTGATACTTCCATTTCATCCAGAAATAGTCGCGATCATCAAGCAAAACCGCACACGACAGATAGACAGGACAGTGAAACCATAGTCCCTTCACATTCAGAATCGTCAAGAAGCAAGGATACTCATCAAGATGGAACGGAAAATGTGAATGAAAAGAAGATAGATGCAGCAAACAAAAGCAACTCCCGGTCTGTTGGGCTGCCAGCTGATTCATCTGCAGTAGAGCCAGTGGTAGCCAAGCCTACTGCTTCAACGAACTCCAAGAAAGGTGGTGGGGGTGAGGGAGGTAATTCCACAACTTCAGCAATAACGAAGTTGACATCGCGACTGAATTTTTTGAAGGAGCGGCGGAGCCAAATAGCAAATGAGCTTCAAGGCCATGGTCCTGCTCAAAATTTGGTTAAAAGTCAGTCGCTCCAATACATGTCAGAGAAGCCGGAGAAACTTAGGAAAGCGGAGAGTCATTCAGAGAGAGGAGGAAAGAGACCGGAAAGCCAACAGCAGCAGCATAGCCTGGACAGAGGAAAATCGGAGAGCCATTTGTCTGTTACCGTGGAGAAAGGTCGAGTTGTAGAGAGTAGTAAACAGAAAACTTCTCCGAGGACGGACACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

918

Amino Acids

99.14

Weight (kDa)

5.12

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 41 - 143 1.1e-11 PH domain
RhoGAP PF00620 201 - 347 4.2e-28 RhoGAP domain
Lzipper-MIP1 PF14389 624 - 704 5.6e-18 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1151
AatII GACGTC 1 cut(s) 167
Acc16I TGCGCA 1 cut(s) 890
AccB7I CCANNNNNTGG 2 cut(s) 68, 446
AccBSI CCGCTC 1 cut(s) 2482
AccII CGCG 2 cut(s) 2169, 2461
AciI CCGC 8 cut(s) 196, 1125, 1704, 2001, 2186, 2482, 2485, 2591
AclWI GGATC 6 cut(s) 218, 399, 525, 668, 1383, 1626
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 4 cut(s) 256, 942, 2467, 2530
AcuI CTGAAG 5 cut(s) 199, 1386, 1842, 1949, 2421
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 3 cut(s) 779, 1687, 1702
AfiI CCNNNNNNNGG 8 cut(s) 65, 68, 101, 446, 1204, 1433, 1673, 1726
AflII CTTAAG 2 cut(s) 371, 1531
AflIII ACRYGT 3 cut(s) 754, 1984, 2559
AjiI CACGTC 2 cut(s) 1315, 1985
AjnI CCWGG 5 cut(s) 73, 167, 264, 1203, 2649
AloI GAACNNNNNNTCC 2 cut(s) 552, 584
Alw21I GWGCWC 2 cut(s) 336, 601
Alw26I GTCTC 4 cut(s) 1235, 1428, 1849, 2614
AlwI GGATC 6 cut(s) 218, 399, 525, 668, 1383, 1626
AlwNI CAGNNNCTG 2 cut(s) 1121, 1927
Ama87I CYCGRG 1 cut(s) 1892
AoxI GGCC 8 cut(s) 70, 128, 346, 576, 1744, 1866, 2128, 2512
ApoI RAATTY 4 cut(s) 256, 942, 2467, 2530
AspLEI GCGC 3 cut(s) 25, 891, 1549
AspS9I GGNCC 7 cut(s) 71, 77, 128, 808, 1193, 2129, 2519
AsuC2I CCSGG 4 cut(s) 788, 806, 914, 2323
AsuHPI GGTGA 3 cut(s) 7, 1100, 2426
AvaI CYCGRG 1 cut(s) 1892
AvaII GGWCC 4 cut(s) 77, 808, 1193, 2519
AxyI CCTNAGG 2 cut(s) 27, 623
BaeGI GKGCMC 1 cut(s) 1992
BanII GRGCYC 1 cut(s) 601
BarI GAAGNNNNNNTAC 4 cut(s) 648, 680, 2239, 2271
BauI CACGAG 2 cut(s) 99, 1454
BbsI GAAGAC 4 cut(s) 413, 1317, 1374, 1868
Bbv12I GWGCWC 2 cut(s) 336, 601
BceAI ACGGC 4 cut(s) 115, 333, 1759, 1881
BcgI CGANNNNNNTGC 4 cut(s) 187, 221, 1862, 1896
BciT130I CCWGG 5 cut(s) 75, 169, 266, 1205, 2651
BciVI GTATCC 1 cut(s) 2248
BclI TGATCA 1 cut(s) 2116
BcnI CCSGG 4 cut(s) 788, 806, 914, 2323
BcoDI GTCTC 4 cut(s) 1235, 1428, 1849, 2614
BfaI CTAG 5 cut(s) 836, 1412, 1550, 1590, 1886
BfmI CTRYAG 6 cut(s) 549, 675, 1503, 1572, 1913, 2352
BfrI CTTAAG 2 cut(s) 371, 1531
BfuI GTATCC 1 cut(s) 2248
BglII AGATCT 1 cut(s) 2026
BlpI GCTNAGC 1 cut(s) 1000
Bme1390I CCNGG 9 cut(s) 75, 169, 266, 788, 806, 914, 1205, 2323, 2651
Bme18I GGWCC 4 cut(s) 77, 808, 1193, 2519
BmeT110I CYCGRG 1 cut(s) 1892
BmgBI CACGTC 2 cut(s) 1315, 1985
BmgT120I GGNCC 7 cut(s) 71, 77, 128, 808, 1193, 2129, 2519
BmiI GGNNCC 6 cut(s) 810, 918, 1023, 1195, 1718, 2489
BmrFI CCNGG 9 cut(s) 75, 169, 266, 788, 806, 914, 1205, 2323, 2651
BmsI GCATC 7 cut(s) 10, 31, 739, 829, 945, 1282, 2290
BpiI GAAGAC 4 cut(s) 413, 1317, 1374, 1868
BpmI CTGGAG 1 cut(s) 1871
Bpu10I CCTNAGC 2 cut(s) 1668, 2043
Bpu1102I GCTNAGC 1 cut(s) 1000
BpuEI CTTGAG 3 cut(s) 1122, 1769, 1796
BpuMI CCSGG 4 cut(s) 788, 806, 914, 2323
BsaBI GATNNNNATC 1 cut(s) 2259
BsaHI GRCGYC 1 cut(s) 164
BsaI GGTCTC 2 cut(s) 1235, 2614
BsaWI WCCGGW 2 cut(s) 987, 2623
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 636, 666
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 68, 101, 446, 1204, 1433, 1673, 1726
Bse1I ACTGG 3 cut(s) 539, 1693, 2363
Bse21I CCTNAGG 2 cut(s) 27, 623
Bse3DI GCAATG 1 cut(s) 1444
Bse8I GATNNNNATC 1 cut(s) 2259
BseBI CCWGG 5 cut(s) 75, 169, 266, 1205, 2651
BseGI GGATG 9 cut(s) 143, 164, 754, 996, 1312, 1399, 1625, 1635, 2153
BseJI GATNNNNATC 1 cut(s) 2259
BseLI CCNNNNNNNGG 8 cut(s) 65, 68, 101, 446, 1204, 1433, 1673, 1726
BseMI GCAATG 1 cut(s) 1444
BseNI ACTGG 3 cut(s) 539, 1693, 2363
BseRI GAGGAG 9 cut(s) 36, 39, 42, 45, 758, 1178, 1416, 2066, 2625
BseSI GKGCMC 1 cut(s) 1992
BseYI CCCAGC 1 cut(s) 1049
BsgI GTGCAG 2 cut(s) 396, 1366
Bsh1236I CGCG 2 cut(s) 2169, 2461
BshFI GGCC 8 cut(s) 72, 130, 348, 578, 1746, 1868, 2130, 2514
BsiHKAI GWGCWC 2 cut(s) 336, 601
BsiHKCI CYCGRG 1 cut(s) 1892
BsiSI CCGG 7 cut(s) 787, 806, 914, 988, 2323, 2573, 2624
BslFI GGGAC 6 cut(s) 794, 1179, 1186, 1710, 1995, 2207
BslI CCNNNNNNNGG 8 cut(s) 65, 68, 101, 446, 1204, 1433, 1673, 1726
BsmAI GTCTC 4 cut(s) 1235, 1428, 1849, 2614
BsmFI GGGAC 6 cut(s) 794, 1179, 1186, 1710, 1995, 2207
BsmI GAATGC 1 cut(s) 1834
BsnI GGCC 8 cut(s) 72, 130, 348, 578, 1746, 1868, 2130, 2514
Bso31I GGTCTC 2 cut(s) 1235, 2614
BsoBI CYCGRG 1 cut(s) 1892
Bsp1286I GDGCHC 3 cut(s) 336, 601, 1992
Bsp1720I GCTNAGC 1 cut(s) 1000
Bsp19I CCATGG 3 cut(s) 1725, 2125, 2515
Bsp68I TCGCGA 2 cut(s) 2169, 2461
BspACI CCGC 8 cut(s) 196, 1125, 1704, 2001, 2186, 2482, 2485, 2591
BspANI GGCC 8 cut(s) 72, 130, 348, 578, 1746, 1868, 2130, 2514
BspFNI CGCG 2 cut(s) 2169, 2461
BspLI GGNNCC 6 cut(s) 810, 918, 1023, 1195, 1718, 2489
BspMAI CTGCAG 2 cut(s) 679, 2356
BspPI GGATC 6 cut(s) 218, 399, 525, 668, 1383, 1626
BspTI CTTAAG 2 cut(s) 371, 1531
BspTNI GGTCTC 2 cut(s) 1235, 2614
BsrBI CCGCTC 1 cut(s) 2482
BsrDI GCAATG 1 cut(s) 1444
BsrI ACTGG 3 cut(s) 539, 1693, 2363
BssNI GRCGYC 1 cut(s) 164
BssSI CACGAG 2 cut(s) 99, 1454
BssT1I CCWWGG 4 cut(s) 579, 1725, 2125, 2515
Bst2BI CACGAG 2 cut(s) 99, 1454
Bst2UI CCWGG 5 cut(s) 75, 169, 266, 1205, 2651
Bst4CI ACNGT 7 cut(s) 331, 553, 1217, 1403, 1923, 2210, 2689
Bst6I CTCTTC 3 cut(s) 1575, 1749, 1809
BstACI GRCGYC 1 cut(s) 164
BstAFI CTTAAG 2 cut(s) 371, 1531
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstC8I GCNNGC 5 cut(s) 576, 1113, 1557, 2096, 2340
BstDSI CCRYGG 4 cut(s) 1725, 2125, 2515, 2688
BstENI CCTNNNNNAGG 2 cut(s) 1202, 1431
BstF5I GGATG 9 cut(s) 143, 164, 754, 996, 1312, 1399, 1625, 1635, 2153
BstFNI CGCG 2 cut(s) 2169, 2461
BstHHI GCGC 3 cut(s) 25, 891, 1549
BstMAI GTCTC 4 cut(s) 1235, 1428, 1849, 2614
BstNI CCWGG 5 cut(s) 75, 169, 266, 1205, 2651
BstNSI RCATGY 4 cut(s) 758, 1502, 2098, 2563
BstSCI CCNGG 9 cut(s) 73, 167, 264, 786, 804, 912, 1203, 2321, 2649
BstSFI CTRYAG 6 cut(s) 549, 675, 1503, 1572, 1913, 2352
BstSLI GKGCMC 1 cut(s) 1992
BstUI CGCG 2 cut(s) 2169, 2461
BstV2I GAAGAC 4 cut(s) 413, 1317, 1374, 1868
BstX2I RGATCY 3 cut(s) 391, 660, 2026
BstYI RGATCY 3 cut(s) 391, 660, 2026
Bsu36I CCTNAGG 2 cut(s) 27, 623
BsuI GTATCC 1 cut(s) 2248
BsuRI GGCC 8 cut(s) 72, 130, 348, 578, 1746, 1868, 2130, 2514
BtgI CCRYGG 4 cut(s) 1725, 2125, 2515, 2688
BtgZI GCGATG 2 cut(s) 54, 2442
BtrI CACGTC 2 cut(s) 1315, 1985
BtsCI GGATG 9 cut(s) 143, 164, 754, 996, 1312, 1399, 1625, 1635, 2153
BtsI GCAGTG 2 cut(s) 301, 1354
BtsIMutI CAGTG 8 cut(s) 301, 336, 546, 1222, 1354, 1604, 2215, 2370
BtuMI TCGCGA 2 cut(s) 2169, 2461
Cac8I GCNNGC 5 cut(s) 576, 1113, 1557, 2096, 2340
CaiI CAGNNNCTG 2 cut(s) 1121, 1927
CfoI GCGC 3 cut(s) 25, 891, 1549
Cfr13I GGNCC 7 cut(s) 71, 77, 128, 808, 1193, 2129, 2519
CseI GACGC 2 cut(s) 804, 1868
Csp6I GTAC 3 cut(s) 778, 1686, 1701
CspCI CAANNNNNGTGG 2 cut(s) 2386, 2421
CviQI GTAC 3 cut(s) 778, 1686, 1701
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 3 cut(s) 1575, 1749, 1809
EarI CTCTTC 3 cut(s) 1575, 1749, 1809
EciI GGCGGA 1 cut(s) 2500
Ecl136II GAGCTC 1 cut(s) 599
Eco130I CCWWGG 4 cut(s) 579, 1725, 2125, 2515
Eco24I GRGCYC 1 cut(s) 601
Eco31I GGTCTC 2 cut(s) 1235, 2614
Eco47I GGWCC 4 cut(s) 77, 808, 1193, 2519
Eco53kI GAGCTC 1 cut(s) 599
Eco57I CTGAAG 5 cut(s) 199, 1386, 1842, 1949, 2421
Eco81I CCTNAGG 2 cut(s) 27, 623
Eco88I CYCGRG 1 cut(s) 1892
EcoICRI GAGCTC 1 cut(s) 599
EcoNI CCTNNNNNAGG 2 cut(s) 1202, 1431
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 5 cut(s) 73, 167, 264, 1203, 2649
EcoT14I CCWWGG 4 cut(s) 579, 1725, 2125, 2515
EcoT38I GRGCYC 1 cut(s) 601
ErhI CCWWGG 4 cut(s) 579, 1725, 2125, 2515
FalI AAGNNNNNCTT 4 cut(s) 308, 340, 1853, 1885
FaqI GGGAC 6 cut(s) 794, 1179, 1186, 1710, 1995, 2207
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 2116
FokI GGATG 9 cut(s) 130, 171, 761, 1003, 1319, 1406, 1612, 1622, 2140
FriOI GRGCYC 1 cut(s) 601
FspAI RTGCGCAY 1 cut(s) 890
FspBI CTAG 5 cut(s) 836, 1412, 1550, 1590, 1886
FspI TGCGCA 1 cut(s) 890
GlaI GCGC 3 cut(s) 24, 890, 1548
GsaI CCCAGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 1871
HaeIII GGCC 8 cut(s) 72, 130, 348, 578, 1746, 1868, 2130, 2514
HapII CCGG 7 cut(s) 787, 806, 914, 988, 2323, 2573, 2624
HgaI GACGC 2 cut(s) 804, 1868
HhaI GCGC 3 cut(s) 25, 891, 1549
Hin1I GRCGYC 1 cut(s) 164
Hin6I GCGC 3 cut(s) 23, 889, 1547
HinP1I GCGC 3 cut(s) 23, 889, 1547
HincII GTYRAC 2 cut(s) 1066, 2454
HindII GTYRAC 2 cut(s) 1066, 2454
HindIII AAGCTT 1 cut(s) 1772
HpaII CCGG 7 cut(s) 787, 806, 914, 988, 2323, 2573, 2624
HphI GGTGA 3 cut(s) 7, 1100, 2426
Hpy166II GTNNAC 7 cut(s) 80, 985, 1040, 1066, 1604, 2454, 2720
Hpy8I GTNNAC 7 cut(s) 80, 985, 1040, 1066, 1604, 2454, 2720
Hpy99I CGWCG 1 cut(s) 1884
HpyCH4III ACNGT 7 cut(s) 331, 553, 1217, 1403, 1923, 2210, 2689
HpyCH4IV ACGT 7 cut(s) 164, 681, 780, 1314, 1343, 1699, 1984
HpySE526I ACGT 7 cut(s) 164, 681, 780, 1314, 1343, 1699, 1984
Hsp92I GRCGYC 1 cut(s) 164
HspAI GCGC 3 cut(s) 23, 889, 1547
Ksp22I TGATCA 1 cut(s) 2116
LmnI GCTCC 8 cut(s) 49, 596, 604, 916, 1027, 2479, 2487, 2556
LweI GCATC 7 cut(s) 10, 31, 739, 829, 945, 1282, 2290
MaeI CTAG 5 cut(s) 836, 1412, 1550, 1590, 1886
MaeII ACGT 7 cut(s) 164, 681, 780, 1314, 1343, 1699, 1984
MaeIII GTNAC 5 cut(s) 429, 824, 1217, 1923, 2683
MbiI CCGCTC 1 cut(s) 2482
MflI RGATCY 3 cut(s) 391, 660, 2026
MhlI GDGCHC 3 cut(s) 336, 601, 1992
MlyI GAGTC 6 cut(s) 654, 706, 885, 1574, 1739, 2605
MmeI TCCRAC 1 cut(s) 1140
MseI TTAA 8 cut(s) 191, 372, 1406, 1479, 1532, 1943, 2103, 2538
MslI CAYNNNNRTG 2 cut(s) 560, 2265
MspA1I CMGCKG 4 cut(s) 1004, 1118, 1706, 2342
MspCI CTTAAG 2 cut(s) 371, 1531
MspI CCGG 7 cut(s) 787, 806, 914, 988, 2323, 2573, 2624
MspR9I CCNGG 9 cut(s) 75, 169, 266, 788, 806, 914, 1205, 2323, 2651
Mva1269I GAATGC 1 cut(s) 1834
MvaI CCWGG 5 cut(s) 75, 169, 266, 1205, 2651
MvnI CGCG 2 cut(s) 2169, 2461
NciI CCSGG 4 cut(s) 788, 806, 914, 2323
NcoI CCATGG 3 cut(s) 1725, 2125, 2515
NlaIV GGNNCC 6 cut(s) 810, 918, 1023, 1195, 1718, 2489
NmuCI GTSAC 2 cut(s) 429, 1217
NruI TCGCGA 2 cut(s) 2169, 2461
NsbI TGCGCA 1 cut(s) 890
NspI RCATGY 4 cut(s) 758, 1502, 2098, 2563
PaeI GCATGC 1 cut(s) 2098
PaeR7I CTCGAG 1 cut(s) 1892
PasI CCCWGGG 1 cut(s) 265
PciI ACATGT 2 cut(s) 754, 2559
PctI GAATGC 1 cut(s) 1834
PfeI GAWTC 6 cut(s) 12, 86, 474, 1681, 2237, 2345
PflMI CCANNNNNTGG 2 cut(s) 68, 446
PfoI TCCNGGA 2 cut(s) 167, 912
PleI GAGTC 6 cut(s) 653, 705, 884, 1573, 1738, 2604
PpsI GAGTC 6 cut(s) 653, 705, 884, 1573, 1738, 2604
PscI ACATGT 2 cut(s) 754, 2559
PsiI TTATAA 1 cut(s) 1151
Psp124BI GAGCTC 1 cut(s) 601
Psp6I CCWGG 5 cut(s) 73, 167, 264, 1203, 2649
PspFI CCCAGC 1 cut(s) 1049
PspGI CCWGG 5 cut(s) 73, 167, 264, 1203, 2649
PspN4I GGNNCC 6 cut(s) 810, 918, 1023, 1195, 1718, 2489
PspPI GGNCC 7 cut(s) 71, 77, 128, 808, 1193, 2129, 2519
PstI CTGCAG 2 cut(s) 679, 2356
PstNI CAGNNNCTG 2 cut(s) 1121, 1927
PsuI RGATCY 3 cut(s) 391, 660, 2026
PvuII CAGCTG 3 cut(s) 1004, 1118, 2342
RruI TCGCGA 2 cut(s) 2169, 2461
RsaI GTAC 3 cut(s) 779, 1687, 1702
RsaNI GTAC 3 cut(s) 778, 1686, 1701
RseI CAYNNNNRTG 2 cut(s) 560, 2265
SacI GAGCTC 1 cut(s) 601
SaqAI TTAA 8 cut(s) 191, 372, 1406, 1479, 1532, 1943, 2103, 2538
Sau96I GGNCC 7 cut(s) 71, 77, 128, 808, 1193, 2129, 2519
SchI GAGTC 6 cut(s) 654, 706, 885, 1574, 1739, 2605
ScrFI CCNGG 9 cut(s) 75, 169, 266, 788, 806, 914, 1205, 2323, 2651
SduI GDGCHC 3 cut(s) 336, 601, 1992
SfaNI GCATC 7 cut(s) 10, 31, 739, 829, 945, 1282, 2290
SfcI CTRYAG 6 cut(s) 549, 675, 1503, 1572, 1913, 2352
Sfr274I CTCGAG 1 cut(s) 1892
SinI GGWCC 4 cut(s) 77, 808, 1193, 2519
SlaI CTCGAG 1 cut(s) 1892
SmiMI CAYNNNNRTG 2 cut(s) 560, 2265
SmlI CTYRAG 6 cut(s) 371, 1137, 1531, 1748, 1775, 1892
SmoI CTYRAG 6 cut(s) 371, 1137, 1531, 1748, 1775, 1892
SphI GCATGC 1 cut(s) 2098
SsiI CCGC 8 cut(s) 196, 1125, 1704, 2001, 2186, 2482, 2485, 2591
SspMI CTAG 5 cut(s) 836, 1412, 1550, 1590, 1886
SstI GAGCTC 1 cut(s) 601
StyD4I CCNGG 9 cut(s) 73, 167, 264, 786, 804, 912, 1203, 2321, 2649
StyI CCWWGG 4 cut(s) 579, 1725, 2125, 2515
TaaI ACNGT 7 cut(s) 331, 553, 1217, 1403, 1923, 2210, 2689
TaiI ACGT 7 cut(s) 167, 684, 783, 1317, 1346, 1702, 1987
TaqI TCGA 4 cut(s) 524, 700, 1893, 2701
TaqII GACCGA 1 cut(s) 1259
TauI GCSGC 3 cut(s) 1127, 2003, 2485
TfiI GAWTC 6 cut(s) 12, 86, 474, 1681, 2237, 2345
Tru1I TTAA 8 cut(s) 191, 372, 1406, 1479, 1532, 1943, 2103, 2538
Tru9I TTAA 8 cut(s) 191, 372, 1406, 1479, 1532, 1943, 2103, 2538
TscAI CASTG 8 cut(s) 301, 336, 546, 1222, 1354, 1611, 2215, 2370
TseFI GTSAC 2 cut(s) 429, 1217
Tsp45I GTSAC 2 cut(s) 429, 1217
TspGWI ACGGA 2 cut(s) 591, 2288
TspRI CASTG 8 cut(s) 301, 336, 546, 1222, 1354, 1611, 2215, 2370
Van91I CCANNNNNTGG 2 cut(s) 68, 446
Vha464I CTTAAG 2 cut(s) 371, 1531
VpaK11BI GGWCC 4 cut(s) 77, 808, 1193, 2519
XagI CCTNNNNNAGG 2 cut(s) 1202, 1431
XapI RAATTY 4 cut(s) 256, 942, 2467, 2530
XbaI TCTAGA 1 cut(s) 1589
XceI RCATGY 4 cut(s) 758, 1502, 2098, 2563
XhoI CTCGAG 1 cut(s) 1892
XspI CTAG 5 cut(s) 836, 1412, 1550, 1590, 1886
ZraI GACGTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.