pycom15g35880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009185
Physical Location & Seq
Reverse (-)
35948201 .. 35950239
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGGCTAGGCACCTTCCAAGTAGCAGTATTAGGTTGTTCTGTGTGGTTGTTTTGGTGATGGTGGTTCTGGAAGCTGGAGTAGTTTGGTGCCAACATGTGAACTCATCATCACCGTCAGCCGGTGGCGGTGGCGGTGGGAGGAGATTGTTGTCAGAAACGGTGCCTGCCATGTTTATATTTGGAGACTCTCTAATTGACAATGGGAATAACAACGACATTCCGTCATTTGCCAAGGCTAACTATTTCCCTTATGGCATTGATTTCAACGGCGGTCCTACTGGCCGTTTTTCCAATGGCTACACCATGGTTGATGAAATATCTGAACTGCTAGGACTTCCCTTGATTCCTGCTCACTCTGAAGCTTCTGGTGATCAAATGCGTCATGGAGTCAATTATGCATCTGCAGCTGCTGGAATCCTTGATGACACTGGCAGAAACTTCGTGGGAAGAATACCTTTTAGTGAACAAATAAGAAACTTTCAGAATACACTTGATCAGATAACAGGCAATCTTGGTGCTGATGATGCTGCTCGATCTATTGCCAAGTGCATCTTCTTCGTTGGAATGGGAAGCAATGACTACCTGAATAATTACCTAATGCCCAACTATCCAACTAGGAATCAGTACAATGCCCAACAATATGCTGATCTCTTGGCTCAAGAATATACACAGCAGCTCACTAGACTTTACAATCTTGGTGCCCGAAAATTTGTTATTGCTGGATTAGGAAGAATGGGTTGCATTCCAAGTATTTTGGCCCAAAATCCAAGTGGAACCTGCTCGGACGGAGTTAATCGCCTTGTTCTACCTTTTAATACAAATGTGAAGACAATGATCAACAATCTTAATGCCAACTTACCGAGCTCAAAGTTCATTTACATAGACATCGCTCGTATGTTCGAAGACATGCTCCTCAATTCTAGATCCTATGGATTCAGCGTTGCAAATCGTGGATGCTGCGGTATTGGGCGTAACAGAGGCCAAGTTACATGTCTTCCTTTCCAAACACCATGCCCAAATCGAGACCAATATATCTTCTGGGATGCATTCCACCCGACATCAGCGGTGAACATCATAATTGGGAGGAAAGCTTTCAGCGGGGACCGTACCGAGGTTTATCCCATGAACATACAGCAGCTTGCCACTCTTGACATTGAAAGGAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.66

Weight (kDa)

6.94

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 362 1.8e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 785
AccB1I GGYRCC 4 cut(s) 9, 87, 160, 698
AciI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
AclWI GGATC 1 cut(s) 918
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 707
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 626, 1108
AfiI CCNNNNNNNGG 2 cut(s) 119, 1111
AflIII ACRYGT 2 cut(s) 94, 989
AgsI TTSAA 2 cut(s) 265, 1157
AhdI GACNNNNNGTC 1 cut(s) 220
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AluBI AGCT 7 cut(s) 74, 362, 407, 676, 864, 1091, 1138
AluI AGCT 7 cut(s) 74, 362, 407, 676, 864, 1091, 1138
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 2 cut(s) 177, 1017
AlwI GGATC 1 cut(s) 918
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 3 cut(s) 280, 756, 979
ApeKI GCWGC 6 cut(s) 404, 407, 527, 673, 957, 1135
ApoI RAATTY 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 1091
AspS9I GGNCC 3 cut(s) 272, 757, 1102
AsuHPI GGTGA 4 cut(s) 67, 102, 380, 1078
AsuII TTCGAA 1 cut(s) 900
AvaII GGWCC 2 cut(s) 272, 1102
BaeGI GKGCMC 1 cut(s) 703
BanI GGYRCC 4 cut(s) 9, 87, 160, 698
BanII GRGCYC 1 cut(s) 866
BbsI GAAGAC 3 cut(s) 833, 909, 986
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BccI CCATC 1 cut(s) 52
BceAI ACGGC 2 cut(s) 267, 283
BcgI CGANNNNNNTGC 4 cut(s) 421, 455, 538, 572
BclI TGATCA 3 cut(s) 370, 493, 834
BcoDI GTCTC 2 cut(s) 177, 1017
BfaI CTAG 5 cut(s) 6, 329, 615, 681, 921
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 785
BisI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
BlsI GCNGC 6 cut(s) 406, 409, 529, 675, 959, 1137
Bme18I GGWCC 2 cut(s) 272, 1102
BmeRI GACNNNNNGTC 1 cut(s) 220
BmgT120I GGNCC 3 cut(s) 272, 757, 1102
BmiI GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
BmsI GCATC 5 cut(s) 407, 514, 558, 944, 1033
BpiI GAAGAC 3 cut(s) 833, 909, 986
BpmI CTGGAG 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 1017
BsaJI CCNNGG 3 cut(s) 231, 303, 1110
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 1111
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 283, 433, 1165
Bse3DI GCAATG 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 231, 303, 1110
BseGI GGATG 2 cut(s) 959, 1048
BseLI CCNNNNNNNGG 2 cut(s) 119, 1111
BseMI GCAATG 1 cut(s) 580
BseNI ACTGG 3 cut(s) 283, 433, 1165
BseRI GAGGAG 2 cut(s) 154, 902
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BshFI GGCC 3 cut(s) 282, 758, 981
BshNI GGYRCC 4 cut(s) 9, 87, 160, 698
BsiHKAI GWGCWC 1 cut(s) 866
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 1115
BslI CCNNNNNNNGG 2 cut(s) 119, 1111
BsmAI GTCTC 2 cut(s) 177, 1017
BsmFI GGGAC 1 cut(s) 1115
BsmI GAATGC 2 cut(s) 741, 1046
BsnI GGCC 3 cut(s) 282, 758, 981
Bso31I GGTCTC 1 cut(s) 1017
Bsp119I TTCGAA 1 cut(s) 900
Bsp1286I GDGCHC 2 cut(s) 703, 866
Bsp143I GATC 6 cut(s) 370, 493, 533, 646, 834, 923
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
BspANI GGCC 3 cut(s) 282, 758, 981
BspLI GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 1 cut(s) 918
BspT104I TTCGAA 1 cut(s) 900
BspT107I GGYRCC 4 cut(s) 9, 87, 160, 698
BspTNI GGTCTC 1 cut(s) 1017
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 283, 433, 1165
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 231, 303, 1110
BssMI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
BssT1I CCWWGG 2 cut(s) 231, 303
Bst4CI ACNGT 3 cut(s) 114, 160, 1106
BstBI TTCGAA 1 cut(s) 900
BstC8I GCNNGC 2 cut(s) 165, 1140
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 2 cut(s) 959, 1048
BstKTI GATC 6 cut(s) 373, 496, 536, 649, 837, 926
BstMAI GTCTC 2 cut(s) 177, 1017
BstMBI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
BstMWI GCNNNNNNNGC 2 cut(s) 404, 524
BstNSI RCATGY 3 cut(s) 98, 910, 993
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BstV2I GAAGAC 3 cut(s) 833, 909, 986
BstX2I RGATCY 1 cut(s) 923
BstYI RGATCY 1 cut(s) 923
BsuRI GGCC 3 cut(s) 282, 758, 981
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 871
BtsCI GGATG 2 cut(s) 959, 1048
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 2 cut(s) 165, 1140
CaiI CAGNNNCTG 1 cut(s) 410
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 272, 757, 1102
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 625, 1107
CviAII CATG 8 cut(s) 95, 169, 304, 383, 907, 990, 1011, 1123
CviQI GTAC 2 cut(s) 625, 1107
DpnI GATC 6 cut(s) 372, 495, 535, 648, 836, 925
DpnII GATC 6 cut(s) 370, 493, 533, 646, 834, 923
DriI GACNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 280
Eam1105I GACNNNNNGTC 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 864
Eco130I CCWWGG 2 cut(s) 231, 303
Eco24I GRGCYC 1 cut(s) 866
Eco31I GGTCTC 1 cut(s) 1017
Eco47I GGWCC 2 cut(s) 272, 1102
Eco53kI GAGCTC 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 864
EcoT14I CCWWGG 2 cut(s) 231, 303
EcoT22I ATGCAT 2 cut(s) 400, 1048
EcoT38I GRGCYC 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 231, 303
FaeI CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
FalI AAGNNNNNCTT 4 cut(s) 439, 471, 536, 568
FaqI GGGAC 1 cut(s) 1115
FatI CATG 8 cut(s) 94, 168, 303, 382, 906, 989, 1010, 1122
FauI CCCGC 1 cut(s) 1091
FbaI TGATCA 3 cut(s) 370, 493, 834
Fnu4HI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
FokI GGATG 2 cut(s) 966, 1055
FriOI GRGCYC 1 cut(s) 866
Fsp4HI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
FspBI CTAG 5 cut(s) 6, 329, 615, 681, 921
GluI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 282, 758, 981
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 368
Hin1II CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
HindIII AAGCTT 2 cut(s) 360, 1089
HinfI GANTC 6 cut(s) 185, 343, 387, 414, 619, 933
HpaII CCGG 1 cut(s) 120
HphI GGTGA 4 cut(s) 67, 102, 380, 1078
Hpy166II GTNNAC 3 cut(s) 100, 464, 1069
Hpy188I TCNGA 6 cut(s) 154, 322, 358, 483, 498, 784
Hpy188III TCNNGA 5 cut(s) 68, 659, 921, 1022, 1148
Hpy8I GTNNAC 3 cut(s) 100, 464, 1069
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 3 cut(s) 114, 160, 1106
HpyCH4V TGCA 6 cut(s) 398, 404, 549, 741, 944, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 524
Hsp92II CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
Ksp22I TGATCA 3 cut(s) 370, 493, 834
Kzo9I GATC 6 cut(s) 370, 493, 533, 646, 834, 923
LmnI GCTCC 1 cut(s) 915
Lsp1109I GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
LweI GCATC 5 cut(s) 407, 514, 558, 944, 1033
MaeI CTAG 5 cut(s) 6, 329, 615, 681, 921
MaeIII GTNAC 2 cut(s) 971, 985
MalI GATC 6 cut(s) 372, 495, 535, 648, 836, 925
MboI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
MboII GAAGA 8 cut(s) 459, 544, 547, 741, 838, 914, 986, 1027
MflI RGATCY 1 cut(s) 923
MhlI GDGCHC 2 cut(s) 703, 866
MluCI AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
MlyI GAGTC 2 cut(s) 179, 396
MmeI TCCRAC 2 cut(s) 541, 635
MnlI CCTC 5 cut(s) 132, 923, 971, 1077, 1105
Mph1103I ATGCAT 2 cut(s) 400, 1048
MroXI GAANNNNTTC 1 cut(s) 1091
MseI TTAA 3 cut(s) 792, 813, 846
MspA1I CMGCKG 3 cut(s) 407, 1064, 1098
MspI CCGG 1 cut(s) 120
Mva1269I GAATGC 2 cut(s) 741, 1046
MwoI GCNNNNNNNGC 2 cut(s) 404, 524
NcoI CCATGG 1 cut(s) 303
NdeII GATC 6 cut(s) 370, 493, 533, 646, 834, 923
NlaIII CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
NlaIV GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
NsiI ATGCAT 2 cut(s) 400, 1048
NspI RCATGY 3 cut(s) 98, 910, 993
NspV TTCGAA 1 cut(s) 900
PciI ACATGT 2 cut(s) 94, 989
PctI GAATGC 2 cut(s) 741, 1046
PdmI GAANNNNTTC 1 cut(s) 1091
PfeI GAWTC 4 cut(s) 343, 414, 619, 933
PkrI GCNGC 6 cut(s) 406, 409, 529, 675, 959, 1137
PleI GAGTC 2 cut(s) 179, 395
PpsI GAGTC 2 cut(s) 179, 395
PscI ACATGT 2 cut(s) 94, 989
Psp124BI GAGCTC 1 cut(s) 866
PspN4I GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
PspPI GGNCC 3 cut(s) 272, 757, 1102
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 923
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 626, 1108
RsaNI GTAC 2 cut(s) 625, 1107
SacI GAGCTC 1 cut(s) 866
SaqAI TTAA 3 cut(s) 792, 813, 846
SatI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
Sau3AI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
Sau96I GGNCC 3 cut(s) 272, 757, 1102
SchI GAGTC 2 cut(s) 179, 396
SduI GDGCHC 2 cut(s) 703, 866
SfaNI GCATC 5 cut(s) 407, 514, 558, 944, 1033
SfcI CTRYAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 900
SinI GGWCC 2 cut(s) 272, 1102
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
SsiI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
SspMI CTAG 5 cut(s) 6, 329, 615, 681, 921
SstI GAGCTC 1 cut(s) 866
StyI CCWWGG 2 cut(s) 231, 303
TaaI ACNGT 3 cut(s) 114, 160, 1106
TaqI TCGA 3 cut(s) 532, 900, 1021
TasI AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 4 cut(s) 343, 414, 619, 933
Tru1I TTAA 3 cut(s) 792, 813, 846
Tru9I TTAA 3 cut(s) 792, 813, 846
TscAI CASTG 1 cut(s) 433
TseI GCWGC 6 cut(s) 404, 407, 527, 673, 957, 1135
TspDTI ATGAA 3 cut(s) 327, 862, 1139
TspGWI ACGGA 2 cut(s) 210, 801
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 272, 1102
XapI RAATTY 1 cut(s) 707
XbaI TCTAGA 1 cut(s) 920
XceI RCATGY 3 cut(s) 98, 910, 993
XcmI CCANNNNNNNNNTGG 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 1091
XspI CTAG 5 cut(s) 6, 329, 615, 681, 921
Zsp2I ATGCAT 2 cut(s) 400, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.