pycom15g36240

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007874
Physical Location & Seq
Reverse (-)
36282799 .. 36286428
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGACGGCCCTCCAGGCGGCGATCTGTGTTCGGTTTGCCATGGCAACTTCGACACTCCTTGTCAGGCCAATTGCTCTCACTGGTTTTGCGGAAATTGTATTATGCTTGTTTGGCATCATGGATCGGCAGTTTGCCCTTGTAAATGCCCGCTGTGTCGTCGTCCCATCACTTTGTTGGTTCCAAGTAGGGCTTCTTCAAGGCAGCTTAATAGGCCGGAGGTTGTGGAGATTCTAAGGAAGGTTGAAAGGTACAATCGTGTTTTTGGAGGTCATTCCAGAAGTCTTCTTCAGAGAGCACAGGACCTTCCTTTTCTCCTGCGGAGGCTGCTGCGAGAACTAATTGATCCTGATAGATCTCTTCCCCTTGTTATAAGGGCTCGTGTTTGGATTGCAATGATAATGGGTGCTGCGTACCTATTCAGTCCTTTTGACTTCATCCCGGAAGCAATATTTGGTATAGCCGGTCTCTTGGATGATTTCTTCGTAGTGCTCATTGTGTTCTTCCATGTTGCTGCCATTTATCGGTCAGTACTCTATCGCCGTCATGGAGGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

186

Amino Acids

20.94

Weight (kDa)

9.06

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 129 - 164 2.7e-09 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AciI CCGC 4 cut(s) 18, 90, 149, 319
AclWI GGATC 2 cut(s) 130, 338
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 3 cut(s) 251, 413, 531
AfiI CCNNNNNNNGG 2 cut(s) 17, 522
AgsI TTSAA 2 cut(s) 198, 245
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 4 cut(s) 175, 207, 435, 467
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw21I GWGCWC 2 cut(s) 298, 492
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 2 cut(s) 130, 338
AoxI GGCC 3 cut(s) 7, 66, 212
ApeKI GCWGC 5 cut(s) 202, 325, 328, 407, 512
AspS9I GGNCC 2 cut(s) 8, 301
AsuC2I CCSGG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 301
BaeI ACNNNNGTAYC 2 cut(s) 241, 274
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 2 cut(s) 178, 210
BauI CACGAG 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 298, 492
BbvI GCAGC 5 cut(s) 214, 312, 315, 394, 499
BccI CCATC 1 cut(s) 173
BceAI ACGGC 2 cut(s) 22, 525
BciT130I CCWGG 1 cut(s) 15
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 470
BglI GCCNNNNNGGC 1 cut(s) 15
BglII AGATCT 1 cut(s) 353
BisI GCNGC 6 cut(s) 19, 203, 326, 329, 408, 513
BlsI GCNGC 6 cut(s) 20, 204, 327, 330, 409, 514
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 2 cut(s) 15, 440
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 8, 301
BmiI GGNNCC 2 cut(s) 180, 553
BmrFI CCNGG 2 cut(s) 15, 440
BmsI GCATC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 522
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 2 cut(s) 435, 478
BseLI CCNNNNNNNGG 2 cut(s) 17, 522
BseMI GCAATG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 5 cut(s) 214, 312, 315, 394, 499
BshFI GGCC 3 cut(s) 9, 68, 214
BsiHKAI GWGCWC 2 cut(s) 298, 492
BsiSI CCGG 3 cut(s) 215, 440, 462
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 2 cut(s) 17, 522
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 3 cut(s) 9, 68, 214
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 298, 379, 492
Bsp143I GATC 4 cut(s) 22, 122, 343, 353
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 4 cut(s) 18, 90, 149, 319
BspANI GGCC 3 cut(s) 9, 68, 214
BspLI GGNNCC 2 cut(s) 180, 553
BspPI GGATC 2 cut(s) 130, 338
BspTNI GGTCTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 22, 122, 343, 353
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 15
Bst6I CTCTTC 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 233
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 435, 478
BstKTI GATC 4 cut(s) 25, 125, 346, 356
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 22, 122, 343, 353
BstMWI GCNNNNNNNGC 4 cut(s) 15, 112, 211, 325
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 2 cut(s) 13, 438
BstV1I GCAGC 5 cut(s) 214, 312, 315, 394, 499
BstV2I GAAGAC 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuRI GGCC 3 cut(s) 9, 68, 214
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 435, 478
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 149
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 2 cut(s) 8, 301
Csp6I GTAC 3 cut(s) 250, 412, 530
CviAII CATG 4 cut(s) 41, 119, 506, 545
CviJI RGCY 9 cut(s) 9, 68, 191, 205, 214, 325, 377, 461, 552
CviKI_1 RGCY 9 cut(s) 9, 68, 191, 205, 214, 325, 377, 461, 552
CviQI GTAC 3 cut(s) 250, 412, 530
DdeI CTNAG 1 cut(s) 233
DpnI GATC 4 cut(s) 24, 124, 345, 355
DpnII GATC 4 cut(s) 22, 122, 343, 353
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 4 cut(s) 44, 122, 509, 548
FaiI YATR 7 cut(s) 42, 104, 120, 371, 458, 507, 546
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 147
FatI CATG 4 cut(s) 40, 118, 505, 544
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 6 cut(s) 19, 203, 326, 329, 408, 513
FokI GGATG 2 cut(s) 422, 485
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 6 cut(s) 19, 203, 326, 329, 408, 513
GluI GCNGC 6 cut(s) 19, 203, 326, 329, 408, 513
HaeIII GGCC 3 cut(s) 9, 68, 214
HapII CCGG 3 cut(s) 215, 440, 462
Hin1II CATG 4 cut(s) 44, 122, 509, 548
HinfI GANTC 1 cut(s) 229
HpaII CCGG 3 cut(s) 215, 440, 462
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 3 cut(s) 276, 347, 555
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 2 cut(s) 232, 314
HpyCH4V TGCA 1 cut(s) 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 112, 211, 325
HpyF3I CTNAG 1 cut(s) 233
Hsp92II CATG 4 cut(s) 44, 122, 509, 548
Kzo9I GATC 4 cut(s) 22, 122, 343, 353
LmnI GCTCC 1 cut(s) 557
Lsp1109I GCAGC 5 cut(s) 214, 312, 315, 394, 499
LweI GCATC 1 cut(s) 124
MalI GATC 4 cut(s) 24, 124, 345, 355
MboI GATC 4 cut(s) 22, 122, 343, 353
MboII GAAGA 6 cut(s) 186, 275, 278, 350, 472, 493
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 3 cut(s) 298, 379, 492
MluCI AATT 3 cut(s) 70, 94, 339
MnlI CCTC 5 cut(s) 21, 211, 260, 315, 542
MseI TTAA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 3 cut(s) 215, 440, 462
MspR9I CCNGG 2 cut(s) 15, 440
MunI CAATTG 1 cut(s) 70
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 4 cut(s) 15, 112, 211, 325
NciI CCSGG 1 cut(s) 440
NcoI CCATGG 1 cut(s) 40
NdeII GATC 4 cut(s) 22, 122, 343, 353
NlaIII CATG 4 cut(s) 44, 122, 509, 548
NlaIV GGNNCC 2 cut(s) 180, 553
PfeI GAWTC 1 cut(s) 229
PfoI TCCNGGA 1 cut(s) 438
PkrI GCNGC 6 cut(s) 20, 204, 327, 330, 409, 514
PpuMI RGGWCCY 1 cut(s) 301
PsiI TTATAA 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 180, 553
PspPI GGNCC 2 cut(s) 8, 301
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 3 cut(s) 251, 413, 531
RsaNI GTAC 3 cut(s) 250, 412, 530
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 6 cut(s) 19, 203, 326, 329, 408, 513
Sau3AI GATC 4 cut(s) 22, 122, 343, 353
Sau96I GGNCC 2 cut(s) 8, 301
ScaI AGTACT 1 cut(s) 531
ScrFI CCNGG 2 cut(s) 15, 440
SduI GDGCHC 3 cut(s) 298, 379, 492
SetI ASST 7 cut(s) 207, 222, 243, 251, 271, 306, 417
SfaNI GCATC 1 cut(s) 124
SinI GGWCC 1 cut(s) 301
Sse9I AATT 3 cut(s) 70, 94, 339
SsiI CCGC 4 cut(s) 18, 90, 149, 319
SspI AATATT 1 cut(s) 450
StyD4I CCNGG 2 cut(s) 13, 438
StyI CCWWGG 1 cut(s) 40
TaqI TCGA 1 cut(s) 51
TaqII GACCGA 1 cut(s) 513
TasI AATT 3 cut(s) 70, 94, 339
TatI WGTACW 1 cut(s) 529
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 86
TseI GCWGC 5 cut(s) 202, 325, 328, 407, 512
TspDTI ATGAA 1 cut(s) 424
TspRI CASTG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.