pycom15g36260

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010209
Physical Location & Seq
Reverse (-)
36296213 .. 36298526
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGATATTCGAGAATGAAAACATTGAAATAGCTCAGGGCCCATATACAAATTCAACTAAAGCCCAAAAGTATGCAGCTCGAAACCTAAACGGACACAGCCCCCAACCAAAATCACAGAAGAATCTCAAAATCGAAGCGCAGAGCAAGAGAGAGAGAAGCAAGAGAGAGAAGAAAAGAATCAAAGCAGAGAGAGAGAGAGAGAGAGAAAGAGGAATAATTCAGGTGGCTACAATGGCAGACAGCAAAGAAATCATCCCACAGCAGTACGACCTGAACGCCAAATGGGACGCGTGTCTCGATCTGACGGTCCGGCGCTTCGTCTACTCTTCCTTGGGCGGTGCCTTTGGCGGCCTCCTTTTGTTCAGGAGTCCTGTGACTCGTTGGACTTCTGTAGCTTTTGGCGCTGGAGTAGGCATTGGATCTGCATACACAGAGTGCTCCCGTTTGTTTGAGGGATCTCCTGCAAAGTTGGCTGCTCCTAAGATTATTGAGGTCCCTGCTCCTCAGGTAGAGCTGATCTCTTCTGATTTTAAATACAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

182

Amino Acids

20.34

Weight (kDa)

9.77

Isoelectric Point (pI)

66.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 80 - 150 1.8e-22 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 290
AciI CCGC 2 cut(s) 336, 348
AclWI GGATC 2 cut(s) 427, 463
AcsI RAATTY 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 435
AfaI GTAC 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 2 cut(s) 26, 54
AluBI AGCT 4 cut(s) 32, 77, 395, 514
AluI AGCT 4 cut(s) 32, 77, 395, 514
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 2 cut(s) 427, 463
AoxI GGCC 2 cut(s) 37, 349
ApaI GGGCCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 74, 473
ApoI RAATTY 1 cut(s) 49
AspLEI GCGC 3 cut(s) 139, 315, 404
AspS9I GGNCC 4 cut(s) 37, 38, 307, 493
AvaII GGWCC 2 cut(s) 307, 493
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 86, 460
BcoDI GTCTC 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 2 cut(s) 316, 405
BisI GCNGC 3 cut(s) 75, 349, 474
BlsI GCNGC 3 cut(s) 76, 350, 475
Bme18I GGWCC 2 cut(s) 307, 493
BmgT120I GGNCC 4 cut(s) 37, 38, 307, 493
BmiI GGNNCC 3 cut(s) 39, 340, 495
BoxI GACNNNNGTC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 1 cut(s) 426
Bpu10I CCTNAGC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 330
Bse21I CCTNAGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 47, 518
BseRI GAGGAG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 2 cut(s) 86, 460
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 2 cut(s) 39, 351
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 2 cut(s) 299, 479
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 2 cut(s) 299, 479
BsnI GGCC 2 cut(s) 39, 351
Bsp120I GGGCCC 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 41, 440
Bsp143I GATC 4 cut(s) 298, 419, 455, 516
BspACI CCGC 2 cut(s) 336, 348
BspANI GGCC 2 cut(s) 39, 351
BspCNI CTCAG 2 cut(s) 46, 517
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 3 cut(s) 39, 340, 495
BspPI GGATC 2 cut(s) 427, 463
BspT107I GGYRCC 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 4 cut(s) 298, 419, 455, 516
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 2 cut(s) 331, 526
BstDEI CTNAG 3 cut(s) 33, 480, 504
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 2 cut(s) 316, 405
BstHHI GCGC 3 cut(s) 139, 315, 404
BstKTI GATC 4 cut(s) 301, 422, 458, 519
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 4 cut(s) 298, 419, 455, 516
BstMWI GCNNNNNNNGC 3 cut(s) 233, 401, 470
BstPAI GACNNNNGTC 1 cut(s) 291
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 86, 460
BstX2I RGATCY 2 cut(s) 419, 455
BstYI RGATCY 2 cut(s) 419, 455
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 2 cut(s) 39, 351
BtsCI GGATG 1 cut(s) 252
CfoI GCGC 3 cut(s) 139, 315, 404
Cfr13I GGNCC 4 cut(s) 37, 38, 307, 493
CpoI CGGWCCG 1 cut(s) 307
CseI GACGC 1 cut(s) 296
Csp6I GTAC 1 cut(s) 265
CspI CGGWCCG 1 cut(s) 307
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 3 cut(s) 33, 480, 504
DpnI GATC 4 cut(s) 300, 421, 457, 518
DpnII GATC 4 cut(s) 298, 419, 455, 516
DraI TTTAAA 1 cut(s) 532
DraIII CACNNNGTG 1 cut(s) 435
Eam1104I CTCTTC 2 cut(s) 331, 526
EarI CTCTTC 2 cut(s) 331, 526
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 307, 493
Eco81I CCTNAGG 1 cut(s) 504
EcoO109I RGGNCCY 2 cut(s) 37, 493
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 330
FaiI YATR 4 cut(s) 43, 45, 72, 427
FaqI GGGAC 2 cut(s) 299, 479
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 3 cut(s) 75, 349, 474
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 75, 349, 474
GlaI GCGC 3 cut(s) 138, 314, 403
GluI GCNGC 3 cut(s) 75, 349, 474
GsuI CTGGAG 1 cut(s) 426
HaeII RGCGCY 2 cut(s) 316, 405
HaeIII GGCC 2 cut(s) 39, 351
HapII CCGG 1 cut(s) 310
HgaI GACGC 1 cut(s) 296
HhaI GCGC 3 cut(s) 139, 315, 404
Hin6I GCGC 3 cut(s) 137, 313, 402
HinP1I GCGC 3 cut(s) 137, 313, 402
HinfI GANTC 4 cut(s) 121, 177, 367, 376
HpaII CCGG 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 2 cut(s) 303, 526
Hpy188III TCNNGA 3 cut(s) 10, 296, 364
Hpy8I GTNNAC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 74, 425, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 401, 470
HpyF3I CTNAG 3 cut(s) 33, 480, 504
HspAI GCGC 3 cut(s) 137, 313, 402
Kzo9I GATC 4 cut(s) 298, 419, 455, 516
LmnI GCTCC 3 cut(s) 443, 481, 505
Lsp1109I GCAGC 2 cut(s) 86, 460
MaeIII GTNAC 1 cut(s) 373
MalI GATC 4 cut(s) 300, 421, 457, 518
MboI GATC 4 cut(s) 298, 419, 455, 516
MboII GAAGA 4 cut(s) 130, 181, 318, 513
MflI RGATCY 2 cut(s) 419, 455
MhlI GDGCHC 2 cut(s) 41, 440
MluCI AATT 2 cut(s) 49, 216
MluI ACGCGT 1 cut(s) 288
MlyI GAGTC 2 cut(s) 370, 376
MmeI TCCRAC 1 cut(s) 362
MnlI CCTC 5 cut(s) 203, 362, 445, 484, 513
MseI TTAA 1 cut(s) 531
MspI CCGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 233, 401, 470
NdeII GATC 4 cut(s) 298, 419, 455, 516
NlaIV GGNNCC 3 cut(s) 39, 340, 495
NmuCI GTSAC 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PfeI GAWTC 2 cut(s) 121, 177
PkrI GCNGC 3 cut(s) 76, 350, 475
PleI GAGTC 2 cut(s) 370, 375
PpsI GAGTC 2 cut(s) 370, 375
PpuMI RGGWCCY 1 cut(s) 493
PshAI GACNNNNGTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 493
PspN4I GGNNCC 3 cut(s) 39, 340, 495
PspOMI GGGCCC 1 cut(s) 37
PspPI GGNCC 4 cut(s) 37, 38, 307, 493
PspPPI RGGWCCY 1 cut(s) 493
PsuI RGATCY 2 cut(s) 419, 455
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
Rsr2I CGGWCCG 1 cut(s) 307
RsrII CGGWCCG 1 cut(s) 307
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 3 cut(s) 75, 349, 474
Sau3AI GATC 4 cut(s) 298, 419, 455, 516
Sau96I GGNCC 4 cut(s) 37, 38, 307, 493
SchI GAGTC 2 cut(s) 370, 376
SduI GDGCHC 2 cut(s) 41, 440
SetI ASST 9 cut(s) 34, 79, 87, 225, 273, 397, 495, 510, 516
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 2 cut(s) 307, 493
Sse9I AATT 2 cut(s) 49, 216
SsiI CCGC 2 cut(s) 336, 348
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 307
TaqI TCGA 4 cut(s) 9, 79, 132, 297
TasI AATT 2 cut(s) 49, 216
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 121, 177
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 2 cut(s) 74, 473
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 105
VpaK11BI GGWCC 2 cut(s) 307, 493
XapI RAATTY 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.