pycom15g36370

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010354
Physical Location & Seq
Reverse (-)
36401154 .. 36403513
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1737 bp
ATGATTCGTACTTACGCAGAAATCAAAACCCAGAAAATGATGAACTTTGTGGCTCTGTTCCTTGTTGTGAGTTCACTGGTAACCGCTGGGGTGTGGTCTCCGTCTGCAAGCCCACCAAATGATCAGACCCAGCAGCACGAGCAGAGGGAAGAAGGTCATCAGCAGCAGCATGCTGGGGATCAGGAAGTCATTGTCAAAAATGGGCACAGGGTGGTTGTCGTGGAGTACGACGAGCATGGCCACCACAAAACCAAAGTCTCAATCTCGCCGGAACACGGCCATCCACCCCGCAGAGAGGCTTCTTCCTTCTCCGAAAATGAAGTATCGTCGCCCTCGAAGATTTCTAGGGCGTTCGACAACTCCATGGAAAACATCAAGGAAGCCGCCTCTGGTCTCGCCAACTTTGGCCAAGGCCATGGCGAGGGTCACAGTCCGAAAGAGCTCATGTGCGATGCCTACGGAAAATGCAAGCACAAGATTGCAAGTGCTATCGAGACCAAAAAGGAGATTTGTAAGGAGACCGCACACGGGGCAAAGGAGGCGGCGCATAAGGTAGGTGAGGCGGCGCACAGGGTAGGCGAGGAGGTCGAAAATGTGTACGAGAGGGCGAAAGATACGGTGTCGGATAAGGCGCACGAGGTTGAGGAAACTGCTAGAGATCCAATGGAAAAAGCAAAGAACGCCTCCAAGACGGCCAAGGACATGGGGGACACGTTGGCTGGAGACATACAGGGGAATGCGTTGAGGCAGCGCGAACACTTGGCGGAAATAGCGAAAGATGCGAAAGAGACTGCGGAGGAAGCAGCTGGTAAGGTGAAGGCTGGAGCGCGGGAGCTCAAAAGTGACTTGGCGGAAAAAGCAAAAGAAGCCAAAGAGACTGCGGAGGAAACAGCTTTTAAGGTGAAGGCTGGAGCGGAGGAGTTCAAAAGTGACTTGGCTGAAAAAGCGAAAGAGACTGCGGAGGAAGCAGCTGGTAAGGTTAAGGCTGGAGCGGAGGAGATCAAAAGTGGGACCCAGAAGAGGCTGCATCAGTTGAGGGACAAAGAAAGAGAGTTCTGGTACGGTGCATTCAGGTCCATAGCATCGGGTGACTTACTGGAGGGTTTGATGTCCGTGGCGCATCTATTCGGGCTGGCTACGGCTTACGGGATGTGCATGTGGATTACGTTCGCGTCGAGCCACGTGTTGGCGGGGAGTCTGCCGCCGCAGCAGTTTGGGATGGTACAGAGCAAGATTTACAAGGTTTATTTCAAGGCCATGGCGTATAGTGTTGGGATGGCATTGTTGGGTCATCTGTGGAGACATAGAAGCTCGTTGTTTCGAGGGAAGTCGGCGGATATGTTGCAGAACTATAACCTTATCGCTGCCCTTTCCATGATCTTATTCAATATGTACTTGGAGCCTCGATCCACTAAGGTGATGTTTGAGAGAATGAAGATCGAGAAGGAAGAAGGAAGAGGAAGAGAGAGAGTACATGTTGATGCCAGAATCAGCAGGGAGGCTGGCCAGCAGCAGCAGCAGACTACCACTGAAGGTGAACCCTCAATCACGACACCTCCACAAGTCTCGAGGAGGGAGCAACAGGTGGTTGAGCCTGATATTGAGAGGCTTAACGAGATGCGTGAGAGGCTGAAGAAGCTAAATACATATTCGTCGTTCCTCAACATCGCGTCACTAATGGCTCTTAGCTGGCATCTTGTGTATCTTGCCCAGCACCTCCCCTGTTCATGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

579

Amino Acids

64.36

Weight (kDa)

6.96

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 377 - 478 3.6e-20 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1186
AccBSI CCGCTC 2 cut(s) 914, 992
AccII CGCG 4 cut(s) 753, 829, 1172, 1670
AclWI GGATC 3 cut(s) 186, 653, 1401
AcoI YGGCCR 5 cut(s) 238, 277, 406, 693, 1504
AcuI CTGAAG 2 cut(s) 1551, 1652
AcvI CACGTG 1 cut(s) 1183
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 7 cut(s) 10, 227, 599, 1061, 1224, 1394, 1473
AfiI CCNNNNNNNGG 6 cut(s) 275, 295, 421, 528, 1020, 1186
AflIII ACRYGT 3 cut(s) 711, 1182, 1474
AgsI TTSAA 3 cut(s) 925, 1252, 1387
AleI CACNNNNGTG 1 cut(s) 1415
AluBI AGCT 8 cut(s) 442, 806, 835, 893, 971, 1311, 1639, 1689
AluI AGCT 8 cut(s) 442, 806, 835, 893, 971, 1311, 1639, 1689
Alw21I GWGCWC 2 cut(s) 444, 837
AlwI GGATC 3 cut(s) 186, 653, 1401
Ama87I CYCGRG 1 cut(s) 1567
AoxI GGCC 7 cut(s) 238, 277, 406, 412, 693, 1254, 1504
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspLEI GCGC 6 cut(s) 547, 568, 634, 753, 829, 1120
AspS9I GGNCC 2 cut(s) 1011, 1074
AsuHPI GGTGA 6 cut(s) 569, 826, 913, 1100, 1429, 1547
AvaI CYCGRG 1 cut(s) 1567
AvaII GGWCC 2 cut(s) 1011, 1074
BaeGI GKGCMC 1 cut(s) 207
BalI TGGCCA 3 cut(s) 240, 408, 1506
BanII GRGCYC 2 cut(s) 444, 837
BauI CACGAG 2 cut(s) 137, 635
BbrPI CACGTG 1 cut(s) 1183
Bbv12I GWGCWC 2 cut(s) 444, 837
BccI CCATC 3 cut(s) 288, 1213, 1270
BceAI ACGGC 4 cut(s) 292, 708, 1155, 1716
BclI TGATCA 1 cut(s) 121
BfaI CTAG 2 cut(s) 345, 654
Bme18I GGWCC 2 cut(s) 1011, 1074
BmeT110I CYCGRG 1 cut(s) 1567
BmgT120I GGNCC 2 cut(s) 1011, 1074
BmiI GGNNCC 3 cut(s) 1012, 1013, 1401
BmsI GCATC 8 cut(s) 442, 769, 1036, 1091, 1129, 1471, 1608, 1702
BpmI CTGGAG 5 cut(s) 741, 843, 930, 1008, 1118
BsaAI YACGTR 1 cut(s) 1183
BsaI GGTCTC 4 cut(s) 102, 398, 488, 512
BsaJI CCNNGG 6 cut(s) 363, 409, 415, 696, 1113, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 1540, 1570
Bsc4I CCNNNNNNNGG 6 cut(s) 275, 295, 421, 528, 1020, 1186
Bse1I ACTGG 2 cut(s) 81, 1101
BseDI CCNNGG 6 cut(s) 363, 409, 415, 696, 1113, 1257
BseGI GGATG 4 cut(s) 280, 1155, 1224, 1281
BseLI CCNNNNNNNGG 6 cut(s) 275, 295, 421, 528, 1020, 1186
BseNI ACTGG 2 cut(s) 81, 1101
BseRI GAGGAG 4 cut(s) 596, 932, 1010, 1585
BseSI GKGCMC 1 cut(s) 207
BseYI CCCAGC 4 cut(s) 86, 129, 173, 1710
Bsh1236I CGCG 4 cut(s) 753, 829, 1172, 1670
BshFI GGCC 7 cut(s) 240, 279, 408, 414, 695, 1256, 1506
BsiHKAI GWGCWC 2 cut(s) 444, 837
BsiHKCI CYCGRG 1 cut(s) 1567
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 3 cut(s) 722, 1024, 1052
BslI CCNNNNNNNGG 6 cut(s) 275, 295, 421, 528, 1020, 1186
BsmFI GGGAC 3 cut(s) 722, 1024, 1052
BsmI GAATGC 2 cut(s) 742, 1067
BsnI GGCC 7 cut(s) 240, 279, 408, 414, 695, 1256, 1506
Bso31I GGTCTC 4 cut(s) 102, 398, 488, 512
BsoBI CYCGRG 1 cut(s) 1567
Bsp1286I GDGCHC 3 cut(s) 207, 444, 837
Bsp143I GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
Bsp19I CCATGG 3 cut(s) 363, 415, 1257
BspANI GGCC 7 cut(s) 240, 279, 408, 414, 695, 1256, 1506
BspFNI CGCG 4 cut(s) 753, 829, 1172, 1670
BspLI GGNNCC 3 cut(s) 1012, 1013, 1401
BspPI GGATC 3 cut(s) 186, 653, 1401
BspTNI GGTCTC 4 cut(s) 102, 398, 488, 512
BsrBI CCGCTC 2 cut(s) 914, 992
BsrI ACTGG 2 cut(s) 81, 1101
BssECI CCNNGG 6 cut(s) 363, 409, 415, 696, 1113, 1257
BssMI GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
BssSI CACGAG 2 cut(s) 137, 635
BssT1I CCWWGG 5 cut(s) 363, 409, 415, 696, 1257
Bst2BI CACGAG 2 cut(s) 137, 635
Bst4CI ACNGT 3 cut(s) 431, 619, 1064
Bst6I CTCTTC 3 cut(s) 1013, 1450, 1456
BstBAI YACGTR 1 cut(s) 1183
BstC8I GCNNGC 7 cut(s) 109, 171, 470, 1134, 1504, 1508, 1691
BstDEI CTNAG 2 cut(s) 1413, 1685
BstDSI CCRYGG 4 cut(s) 363, 415, 1113, 1257
BstEII GGTNACC 1 cut(s) 79
BstF5I GGATG 4 cut(s) 280, 1155, 1224, 1281
BstFNI CGCG 4 cut(s) 753, 829, 1172, 1670
BstHHI GCGC 6 cut(s) 547, 568, 634, 753, 829, 1120
BstKTI GATC 7 cut(s) 124, 181, 661, 1002, 1380, 1409, 1440
BstMBI GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
BstNSI RCATGY 3 cut(s) 173, 1159, 1478
BstPI GGTNACC 1 cut(s) 79
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 4 cut(s) 753, 829, 1172, 1670
BstX2I RGATCY 1 cut(s) 658
BstXI CCANNNNNNTGG 2 cut(s) 416, 703
BstYI RGATCY 1 cut(s) 658
BsuRI GGCC 7 cut(s) 240, 279, 408, 414, 695, 1256, 1506
BtgI CCRYGG 4 cut(s) 363, 415, 1113, 1257
BtgZI GCGATG 2 cut(s) 465, 1651
BtsCI GGATG 4 cut(s) 280, 1155, 1224, 1281
BtsIMutI CAGTG 2 cut(s) 74, 1527
Cac8I GCNNGC 7 cut(s) 109, 171, 470, 1134, 1504, 1508, 1691
CfoI GCGC 6 cut(s) 547, 568, 634, 753, 829, 1120
Cfr13I GGNCC 2 cut(s) 1011, 1074
CseI GACGC 2 cut(s) 1161, 1659
Csp6I GTAC 7 cut(s) 9, 226, 598, 1060, 1223, 1393, 1472
CviQI GTAC 7 cut(s) 9, 226, 598, 1060, 1223, 1393, 1472
DdeI CTNAG 2 cut(s) 1413, 1685
DpnI GATC 7 cut(s) 123, 180, 660, 1001, 1379, 1408, 1439
DpnII GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
DraIII CACNNNGTG 1 cut(s) 211
EaeI YGGCCR 5 cut(s) 238, 277, 406, 693, 1504
Eam1104I CTCTTC 3 cut(s) 1013, 1450, 1456
EarI CTCTTC 3 cut(s) 1013, 1450, 1456
EciI GGCGGA 3 cut(s) 779, 866, 1349
Ecl136II GAGCTC 2 cut(s) 442, 835
Eco130I CCWWGG 5 cut(s) 363, 409, 415, 696, 1257
Eco24I GRGCYC 2 cut(s) 444, 837
Eco31I GGTCTC 4 cut(s) 102, 398, 488, 512
Eco47I GGWCC 2 cut(s) 1011, 1074
Eco53kI GAGCTC 2 cut(s) 442, 835
Eco57I CTGAAG 2 cut(s) 1551, 1652
Eco72I CACGTG 1 cut(s) 1183
Eco88I CYCGRG 1 cut(s) 1567
Eco91I GGTNACC 1 cut(s) 79
EcoICRI GAGCTC 2 cut(s) 442, 835
EcoO109I RGGNCCY 1 cut(s) 1011
EcoO65I GGTNACC 1 cut(s) 79
EcoT14I CCWWGG 5 cut(s) 363, 409, 415, 696, 1257
EcoT38I GRGCYC 2 cut(s) 444, 837
ErhI CCWWGG 5 cut(s) 363, 409, 415, 696, 1257
FaqI GGGAC 3 cut(s) 722, 1024, 1052
FauI CCCGC 3 cut(s) 296, 822, 1183
FbaI TGATCA 1 cut(s) 121
FokI GGATG 4 cut(s) 267, 1162, 1231, 1288
FriOI GRGCYC 2 cut(s) 444, 837
FspBI CTAG 2 cut(s) 345, 654
GlaI GCGC 6 cut(s) 546, 567, 633, 752, 828, 1119
GsaI CCCAGC 4 cut(s) 90, 133, 177, 1714
GsuI CTGGAG 5 cut(s) 741, 843, 930, 1008, 1118
HaeIII GGCC 7 cut(s) 240, 279, 408, 414, 695, 1256, 1506
HapII CCGG 1 cut(s) 269
HgaI GACGC 2 cut(s) 1161, 1659
HhaI GCGC 6 cut(s) 547, 568, 634, 753, 829, 1120
Hin6I GCGC 6 cut(s) 545, 566, 632, 751, 827, 1118
HinP1I GCGC 6 cut(s) 545, 566, 632, 751, 827, 1118
HinfI GANTC 3 cut(s) 4, 1195, 1488
HpaII CCGG 1 cut(s) 269
HphI GGTGA 6 cut(s) 569, 826, 913, 1100, 1429, 1547
Hpy166II GTNNAC 3 cut(s) 74, 598, 1538
Hpy188I TCNGA 4 cut(s) 126, 313, 435, 625
Hpy188III TCNNGA 5 cut(s) 182, 493, 1441, 1549, 1567
Hpy8I GTNNAC 3 cut(s) 74, 598, 1538
Hpy99I CGWCG 4 cut(s) 233, 331, 1177, 1657
HpyAV CCTTC 7 cut(s) 146, 316, 811, 898, 1438, 1445, 1526
HpyCH4III ACNGT 3 cut(s) 431, 619, 1064
HpyCH4IV ACGT 3 cut(s) 713, 1166, 1182
HpyCH4V TGCA 7 cut(s) 107, 468, 482, 1027, 1067, 1155, 1345
HpyF3I CTNAG 2 cut(s) 1413, 1685
HpySE526I ACGT 3 cut(s) 713, 1166, 1182
HspAI GCGC 6 cut(s) 545, 566, 632, 751, 827, 1118
KflI GGGWCCC 1 cut(s) 1011
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
LmnI GCTCC 6 cut(s) 824, 832, 911, 989, 1399, 1576
LweI GCATC 8 cut(s) 442, 769, 1036, 1091, 1129, 1471, 1608, 1702
MaeI CTAG 2 cut(s) 345, 654
MaeII ACGT 3 cut(s) 713, 1166, 1182
MaeIII GTNAC 6 cut(s) 79, 425, 842, 929, 1088, 1671
MalI GATC 7 cut(s) 123, 180, 660, 1001, 1379, 1408, 1439
MbiI CCGCTC 2 cut(s) 914, 992
MboI GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
MboII GAAGA 9 cut(s) 161, 294, 349, 1030, 1447, 1460, 1467, 1473, 1645
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 3 cut(s) 207, 444, 837
MlsI TGGCCA 3 cut(s) 240, 408, 1506
MluNI TGGCCA 3 cut(s) 240, 408, 1506
MlyI GAGTC 1 cut(s) 1204
MmeI TCCRAC 1 cut(s) 603
Mox20I TGGCCA 3 cut(s) 240, 408, 1506
MscI TGGCCA 3 cut(s) 240, 408, 1506
MseI TTAA 3 cut(s) 897, 981, 1611
MslI CAYNNNNRTG 2 cut(s) 1415, 1479
Msp20I TGGCCA 3 cut(s) 240, 408, 1506
MspA1I CMGCKG 3 cut(s) 86, 806, 971
MspI CCGG 1 cut(s) 269
Mva1269I GAATGC 2 cut(s) 742, 1067
MvnI CGCG 4 cut(s) 753, 829, 1172, 1670
NcoI CCATGG 3 cut(s) 363, 415, 1257
NdeII GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
NlaIV GGNNCC 3 cut(s) 1012, 1013, 1401
NmuCI GTSAC 5 cut(s) 425, 842, 929, 1088, 1671
NspI RCATGY 3 cut(s) 173, 1159, 1478
OliI CACNNNNGTG 1 cut(s) 1415
PaeI GCATGC 1 cut(s) 173
PaeR7I CTCGAG 1 cut(s) 1567
PciI ACATGT 1 cut(s) 1474
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 332, 1172
PctI GAATGC 2 cut(s) 742, 1067
PfeI GAWTC 2 cut(s) 4, 1488
PflMI CCANNNNNTGG 1 cut(s) 1186
PleI GAGTC 1 cut(s) 1203
PmaCI CACGTG 1 cut(s) 1183
PmlI CACGTG 1 cut(s) 1183
PpsI GAGTC 1 cut(s) 1203
Ppu21I YACGTR 1 cut(s) 1183
PpuMI RGGWCCY 1 cut(s) 1011
PscI ACATGT 1 cut(s) 1474
Psp124BI GAGCTC 2 cut(s) 444, 837
Psp5II RGGWCCY 1 cut(s) 1011
PspCI CACGTG 1 cut(s) 1183
PspEI GGTNACC 1 cut(s) 79
PspFI CCCAGC 4 cut(s) 86, 129, 173, 1710
PspN4I GGNNCC 3 cut(s) 1012, 1013, 1401
PspPI GGNCC 2 cut(s) 1011, 1074
PspPPI RGGWCCY 1 cut(s) 1011
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 2 cut(s) 806, 971
RsaI GTAC 7 cut(s) 10, 227, 599, 1061, 1224, 1394, 1473
RsaNI GTAC 7 cut(s) 9, 226, 598, 1060, 1223, 1393, 1472
RseI CAYNNNNRTG 2 cut(s) 1415, 1479
SacI GAGCTC 2 cut(s) 444, 837
SaqAI TTAA 3 cut(s) 897, 981, 1611
Sau3AI GATC 7 cut(s) 121, 178, 658, 999, 1377, 1406, 1437
Sau96I GGNCC 2 cut(s) 1011, 1074
SchI GAGTC 1 cut(s) 1204
SduI GDGCHC 3 cut(s) 207, 444, 837
SfaNI GCATC 8 cut(s) 442, 769, 1036, 1091, 1129, 1471, 1608, 1702
Sfr274I CTCGAG 1 cut(s) 1567
SinI GGWCC 2 cut(s) 1011, 1074
SlaI CTCGAG 1 cut(s) 1567
SmiMI CAYNNNNRTG 2 cut(s) 1415, 1479
SmlI CTYRAG 1 cut(s) 1567
SmoI CTYRAG 1 cut(s) 1567
SphI GCATGC 1 cut(s) 173
SspMI CTAG 2 cut(s) 345, 654
SstI GAGCTC 2 cut(s) 444, 837
StyI CCWWGG 5 cut(s) 363, 409, 415, 696, 1257
TaaI ACNGT 3 cut(s) 431, 619, 1064
TaiI ACGT 3 cut(s) 716, 1169, 1185
TaqI TCGA 9 cut(s) 335, 354, 492, 588, 1175, 1321, 1405, 1440, 1568
TatI WGTACW 2 cut(s) 1392, 1471
TauI GCSGC 5 cut(s) 386, 545, 566, 1204, 1207
TfiI GAWTC 2 cut(s) 4, 1488
Tru1I TTAA 3 cut(s) 897, 981, 1611
Tru9I TTAA 3 cut(s) 897, 981, 1611
TscAI CASTG 2 cut(s) 81, 1534
TseFI GTSAC 5 cut(s) 425, 842, 929, 1088, 1671
Tsp45I GTSAC 5 cut(s) 425, 842, 929, 1088, 1671
TspDTI ATGAA 4 cut(s) 56, 333, 1448, 1716
TspGWI ACGGA 3 cut(s) 90, 474, 1102
TspRI CASTG 2 cut(s) 81, 1534
Van91I CCANNNNNTGG 1 cut(s) 1186
VpaK11BI GGWCC 2 cut(s) 1011, 1074
XceI RCATGY 3 cut(s) 173, 1159, 1478
XhoI CTCGAG 1 cut(s) 1567
XspI CTAG 2 cut(s) 345, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.