Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0014294

Physical Location & Seq

Genomic Coordinates Reverse (-)

931248 .. 932520

Gene Structure 1273 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGCAAGTAAAAGGCGTGCAGGTTGAGGAGCTGTATTCATTGGACCTTGATTCTCTGAACAATCTGAGGCCAGTGTATGGTTTAATTTTCCTATTCAAATGGCGCCCAGGGGATAAGGATGACCGCCTTGTAATTAAGGATCCAAATCCTAATTTGTTTTTCGCAAGCCAGGTTATTAATAATGCATGTGCAACCCAAGCAATCTTGTCTATCCTCTTGAACTGTCCGGACGTTGACATTGGTCCTGAACTGTCGAAACTGAAAGAATTTACGAAGAACTTTCCACCTGAACTGAAAGGATTGGCAATAAACAATAGTGAAGCTATCCGTGCAGCTCATAATAGTTTTGCCAGGCCTGAGCCGTTTGTTTCTGATGAGAAGAAGGTAGCTGACAAAGATGATGATGTCTACCATTTTATAAGCTACTTGCCTGTGGATGGAGTCCTGTATGAGCTTGATGGACTGAAGGAGGGGCCCATTAGCCTTGGACAGTGCCCCAGCGGGCAAGGTGACATGGAATGGCTGCAGATGGTGCAACCTGTAATCCAGGAACGCATCGAAAGGTATTCCAAAAGTGAGATAAGGTTCAATCTCTTGGCCATTATCAAGAACAGGAAGGAGATGTATACTGCTGAGCTCAAGGAACTGCAAAAGAAGAGGGAGCGTATTTTGCAGCAGCTGTCTGCCTTACAGCAAGAGAGAGCAGCCGACGGGAGCAAGTTTGAAGCGCTGAACAAAGCTCTTTCGGAAATCAATGCTGGGATCGAGGGTGCCACCGAGAAGATCTTGATGGAAGAAGAGAAATTTAAGAAGTGGAGAACAGAGAACATTCGCAGGAAGCACAACTACATTCCATTTCTGTTTAACTTCCTCAAGATTCTTGCCGAGAAGAAGCAGTTGAAGCCTCTTATTGAGAAGGCGAAGCAGAAGGCAGGCAACACCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003824 GO:0004843 GO:0004857 GO:0004866 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009892 GO:0009894 GO:0009987 GO:0010016 GO:0010466 GO:0010605 GO:0010951 GO:0016579 GO:0016787 GO:0019222 GO:0019538 GO:0019783 GO:0030162 GO:0030234 GO:0030414 GO:0031011 GO:0031323 GO:0031324 GO:0031329 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033202 GO:0036211 GO:0036459 GO:0042176 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044877 GO:0045861 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0061136 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070603 GO:0070628 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0097346 GO:0098772 GO:0101005 GO:0140096 GO:1901564 GO:1902494 GO:1903050 GO:1903362 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C12

Protein Analysis

316

Amino Acids

36.14

Weight (kDa)

8.22

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C12	PF01088	2 - 187	6e-55	Ubiquitin carboxyl-terminal hydrolase, family 1
UCH_C	PF18031	256 - 300	1e-19	Ubiquitin carboxyl-terminal hydrolases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65650 AT5G16310
fragaria_vesca	FvH4_4g33330 FvH4_4g33330
malus_domestica	MD13G1018500.v1.1 MD16G1016200.v1.1
prunus_persica	Prupe.1G335400_v2.0.a1 Prupe.1G335400_v2.0.a1
pyrus_communis	pycom13g01570 pycom16g01510
rosa_chinensis	RchiOBHm_Chr4g0442561
rosa_laevigata	RLG00000006002
rosa_multiflora	Rmu_ssc0000204.1_g000045
rosa_roxburghii	Rroxscaffold_5G00383370
rosa_rugosa	Rorug04G0341200
rosa_samantha	Rh4AG393600
rosa_wichuraiana	Rw4G034010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	419
Acc36I	ACCTGC	1 cut(s)	10
AccB1I	GGYRCC	2 cut(s)	102, 770
AccB7I	CCANNNNNTGG	1 cut(s)	77
AccI	GTMKAC	2 cut(s)	408, 626
AccIII	TCCGGA	1 cut(s)	226
AciI	CCGC	2 cut(s)	124, 501
AclWI	GGATC	3 cut(s)	134, 147, 770
AcoI	YGGCCR	1 cut(s)	597
AcsI	RAATTY	2 cut(s)	266, 803
AcuI	CTGAAG	1 cut(s)	485
AcyI	GRCGYC	1 cut(s)	103
AfeI	AGCGCT	1 cut(s)	729
AfiI	CCNNNNNNNGG	2 cut(s)	77, 437
AgsI	TTSAA	5 cut(s)	97, 220, 589, 725, 901
AjnI	CCWGG	4 cut(s)	106, 168, 350, 546
AluBI	AGCT	9 cut(s)	31, 323, 335, 389, 423, 454, 637, 679, 740
AluI	AGCT	9 cut(s)	31, 323, 335, 389, 423, 454, 637, 679, 740
Alw21I	GWGCWC	1 cut(s)	639
AlwI	GGATC	3 cut(s)	134, 147, 770
AlwNI	CAGNNNCTG	1 cut(s)	679
Aor13HI	TCCGGA	1 cut(s)	226
Aor51HI	AGCGCT	1 cut(s)	729
AoxI	GGCC	4 cut(s)	68, 353, 473, 597
ApaI	GGGCCC	1 cut(s)	477
ApeKI	GCWGC	5 cut(s)	332, 523, 673, 676, 704
ApoI	RAATTY	2 cut(s)	266, 803
AseI	ATTAAT	1 cut(s)	177
AspLEI	GCGC	2 cut(s)	105, 730
AspS9I	GGNCC	4 cut(s)	43, 242, 473, 474
AsuHPI	GGTGA	1 cut(s)	521
AvaII	GGWCC	2 cut(s)	43, 242
BaeGI	GKGCMC	2 cut(s)	477, 497
BalI	TGGCCA	1 cut(s)	599
BamHI	GGATCC	1 cut(s)	139
BanI	GGYRCC	2 cut(s)	102, 770
BanII	GRGCYC	2 cut(s)	477, 639
BarI	GAAGNNNNNNTAC	2 cut(s)	830, 862
Bbv12I	GWGCWC	1 cut(s)	639
BbvI	GCAGC	5 cut(s)	344, 510, 685, 688, 716
BccI	CCATC	4 cut(s)	431, 452, 523, 784
BceAI	ACGGC	1 cut(s)	346
BciT130I	CCWGG	4 cut(s)	108, 170, 352, 548
BfmI	CTRYAG	1 cut(s)	524
BfoI	RGCGCY	2 cut(s)	106, 731
BfuAI	ACCTGC	1 cut(s)	10
BglII	AGATCT	1 cut(s)	783
BisI	GCNGC	5 cut(s)	333, 524, 674, 677, 705
BlpI	GCTNAGC	1 cut(s)	633
BlsI	GCNGC	5 cut(s)	334, 525, 675, 678, 706
Bme1390I	CCNGG	4 cut(s)	108, 170, 352, 548
Bme18I	GGWCC	2 cut(s)	43, 242
BmgT120I	GGNCC	4 cut(s)	43, 242, 473, 474
BmiI	GGNNCC	5 cut(s)	104, 141, 474, 475, 772
BmrFI	CCNGG	4 cut(s)	108, 170, 352, 548
BmsI	GCATC	1 cut(s)	564
BoxI	GACNNNNGTC	1 cut(s)	240
Bpu10I	CCTNAGC	1 cut(s)	357
Bpu1102I	GCTNAGC	1 cut(s)	633
BpuEI	CTTGAG	2 cut(s)	623, 857
BsaBI	GATNNNNATC	1 cut(s)	144
BsaHI	GRCGYC	1 cut(s)	103
BsaJI	CCNNGG	3 cut(s)	106, 107, 484
BsaWI	WCCGGW	1 cut(s)	226
Bsc4I	CCNNNNNNNGG	2 cut(s)	77, 437
Bse1I	ACTGG	1 cut(s)	71
Bse8I	GATNNNNATC	1 cut(s)	144
BseAI	TCCGGA	1 cut(s)	226
BseBI	CCWGG	4 cut(s)	108, 170, 352, 548
BseDI	CCNNGG	3 cut(s)	106, 107, 484
BseGI	GGATG	2 cut(s)	124, 442
BseJI	GATNNNNATC	1 cut(s)	144
BseLI	CCNNNNNNNGG	2 cut(s)	77, 437
BseMII	CTCAG	3 cut(s)	56, 348, 624
BseNI	ACTGG	1 cut(s)	71
BseRI	GAGGAG	1 cut(s)	41
BseSI	GKGCMC	2 cut(s)	477, 497
BseXI	GCAGC	5 cut(s)	344, 510, 685, 688, 716
BseYI	CCCAGC	2 cut(s)	497, 758
BsgI	GTGCAG	2 cut(s)	38, 351
BshFI	GGCC	4 cut(s)	70, 355, 475, 599
BshNI	GGYRCC	2 cut(s)	102, 770
BsiHKAI	GWGCWC	1 cut(s)	639
BsiSI	CCGG	1 cut(s)	227
BslI	CCNNNNNNNGG	2 cut(s)	77, 437
BsnI	GGCC	4 cut(s)	70, 355, 475, 599
Bsp120I	GGGCCC	1 cut(s)	473
Bsp1286I	GDGCHC	3 cut(s)	477, 497, 639
Bsp13I	TCCGGA	1 cut(s)	226
Bsp143I	GATC	3 cut(s)	139, 762, 783
Bsp1720I	GCTNAGC	1 cut(s)	633
BspACI	CCGC	2 cut(s)	124, 501
BspANI	GGCC	4 cut(s)	70, 355, 475, 599
BspCNI	CTCAG	3 cut(s)	57, 349, 625
BspEI	TCCGGA	1 cut(s)	226
BspLI	GGNNCC	5 cut(s)	104, 141, 474, 475, 772
BspMAI	CTGCAG	1 cut(s)	528
BspMI	ACCTGC	1 cut(s)	10
BspPI	GGATC	3 cut(s)	134, 147, 770
BspT107I	GGYRCC	2 cut(s)	102, 770
BsrI	ACTGG	1 cut(s)	71
BssECI	CCNNGG	3 cut(s)	106, 107, 484
BssMI	GATC	3 cut(s)	139, 762, 783
BssNAI	GTATAC	1 cut(s)	627
BssNI	GRCGYC	1 cut(s)	103
BssT1I	CCWWGG	1 cut(s)	484
Bst1107I	GTATAC	1 cut(s)	627
Bst2UI	CCWGG	4 cut(s)	108, 170, 352, 548
Bst4CI	ACNGT	3 cut(s)	224, 252, 492
Bst6I	CTCTTC	2 cut(s)	650, 792
BstACI	GRCGYC	1 cut(s)	103
BstAPI	GCANNNNNTGC	1 cut(s)	532
BstC8I	GCNNGC	4 cut(s)	17, 166, 503, 934
BstDEI	CTNAG	3 cut(s)	65, 357, 633
BstF5I	GGATG	2 cut(s)	124, 442
BstH2I	RGCGCY	2 cut(s)	106, 731
BstHHI	GCGC	2 cut(s)	105, 730
BstKTI	GATC	3 cut(s)	142, 765, 786
BstMBI	GATC	3 cut(s)	139, 762, 783
BstMWI	GCNNNNNNNGC	5 cut(s)	197, 329, 532, 670, 901
BstNI	CCWGG	4 cut(s)	108, 170, 352, 548
BstNSI	RCATGY	1 cut(s)	189
BstPAI	GACNNNNGTC	1 cut(s)	240
BstSCI	CCNGG	4 cut(s)	106, 168, 350, 546
BstSFI	CTRYAG	1 cut(s)	524
BstSLI	GKGCMC	2 cut(s)	477, 497
BstV1I	GCAGC	5 cut(s)	344, 510, 685, 688, 716
BstX2I	RGATCY	2 cut(s)	139, 783
BstYI	RGATCY	2 cut(s)	139, 783
BstZ17I	GTATAC	1 cut(s)	627
BsuRI	GGCC	4 cut(s)	70, 355, 475, 599
BtsCI	GGATG	2 cut(s)	124, 442
BtsIMutI	CAGTG	2 cut(s)	78, 497
BveI	ACCTGC	1 cut(s)	10
Cac8I	GCNNGC	4 cut(s)	17, 166, 503, 934
CaiI	CAGNNNCTG	1 cut(s)	679
CfoI	GCGC	2 cut(s)	105, 730
Cfr13I	GGNCC	4 cut(s)	43, 242, 473, 474
CviAII	CATG	2 cut(s)	186, 514
DdeI	CTNAG	3 cut(s)	65, 357, 633
DinI	GGCGCC	1 cut(s)	104
DpnI	GATC	3 cut(s)	141, 764, 785
DpnII	GATC	3 cut(s)	139, 762, 783
EaeI	YGGCCR	1 cut(s)	597
Eam1104I	CTCTTC	2 cut(s)	650, 792
EarI	CTCTTC	2 cut(s)	650, 792
Ecl136II	GAGCTC	1 cut(s)	637
Eco130I	CCWWGG	1 cut(s)	484
Eco147I	AGGCCT	1 cut(s)	355
Eco24I	GRGCYC	2 cut(s)	477, 639
Eco47I	GGWCC	2 cut(s)	43, 242
Eco47III	AGCGCT	1 cut(s)	729
Eco53kI	GAGCTC	1 cut(s)	637
Eco57I	CTGAAG	1 cut(s)	485
EcoICRI	GAGCTC	1 cut(s)	637
EcoO109I	RGGNCCY	1 cut(s)	473
EcoRII	CCWGG	4 cut(s)	106, 168, 350, 546
EcoT14I	CCWWGG	1 cut(s)	484
EcoT22I	ATGCAT	1 cut(s)	187
EcoT38I	GRGCYC	2 cut(s)	477, 639
EgeI	GGCGCC	1 cut(s)	104
EheI	GGCGCC	1 cut(s)	104
ErhI	CCWWGG	1 cut(s)	484
FaeI	CATG	2 cut(s)	189, 517
FaiI	YATR	7 cut(s)	78, 187, 339, 419, 450, 515, 627
FatI	CATG	2 cut(s)	185, 513
FauI	CCCGC	1 cut(s)	494
FblI	GTMKAC	2 cut(s)	408, 626
Fnu4HI	GCNGC	5 cut(s)	333, 524, 674, 677, 705
FokI	GGATG	2 cut(s)	131, 449
FriOI	GRGCYC	2 cut(s)	477, 639
Fsp4HI	GCNGC	5 cut(s)	333, 524, 674, 677, 705
GlaI	GCGC	2 cut(s)	104, 729
GluI	GCNGC	5 cut(s)	333, 524, 674, 677, 705
GsaI	CCCAGC	2 cut(s)	501, 762
HaeII	RGCGCY	2 cut(s)	106, 731
HaeIII	GGCC	4 cut(s)	70, 355, 475, 599
HapII	CCGG	1 cut(s)	227
HhaI	GCGC	2 cut(s)	105, 730
Hin1I	GRCGYC	1 cut(s)	103
Hin1II	CATG	2 cut(s)	189, 517
Hin6I	GCGC	2 cut(s)	103, 728
HinP1I	GCGC	2 cut(s)	103, 728
HincII	GTYRAC	1 cut(s)	235
HindII	GTYRAC	1 cut(s)	235
HinfI	GANTC	3 cut(s)	50, 441, 877
HpaII	CCGG	1 cut(s)	227
HphI	GGTGA	1 cut(s)	521
Hpy166II	GTNNAC	3 cut(s)	235, 409, 627
Hpy188I	TCNGA	4 cut(s)	57, 66, 373, 748
Hpy188III	TCNNGA	6 cut(s)	217, 227, 245, 607, 787, 874
Hpy8I	GTNNAC	3 cut(s)	235, 409, 627
Hpy99I	CGWCG	1 cut(s)	713
HpyAV	CCTTC	5 cut(s)	376, 460, 610, 910, 922
HpyCH4III	ACNGT	3 cut(s)	224, 252, 492
HpyCH4IV	ACGT	1 cut(s)	231
HpyCH4V	TGCA	9 cut(s)	4, 19, 185, 191, 332, 526, 535, 649, 673
HpyF10VI	GCNNNNNNNGC	5 cut(s)	197, 329, 532, 670, 901
HpyF3I	CTNAG	3 cut(s)	65, 357, 633
HpySE526I	ACGT	1 cut(s)	231
Hsp92I	GRCGYC	1 cut(s)	103
Hsp92II	CATG	2 cut(s)	189, 517
HspAI	GCGC	2 cut(s)	103, 728
KasI	GGCGCC	1 cut(s)	102
Kpn2I	TCCGGA	1 cut(s)	226
Kzo9I	GATC	3 cut(s)	139, 762, 783
LmnI	GCTCC	3 cut(s)	28, 661, 714
Lsp1109I	GCAGC	5 cut(s)	344, 510, 685, 688, 716
LweI	GCATC	1 cut(s)	564
MaeII	ACGT	1 cut(s)	231
MaeIII	GTNAC	1 cut(s)	509
MalI	GATC	3 cut(s)	141, 764, 785
MboI	GATC	3 cut(s)	139, 762, 783
MboII	GAAGA	7 cut(s)	286, 391, 667, 793, 806, 809, 901
MflI	RGATCY	2 cut(s)	139, 783
MhlI	GDGCHC	3 cut(s)	477, 497, 639
MlsI	TGGCCA	1 cut(s)	599
MluCI	AATT	5 cut(s)	84, 132, 151, 266, 803
MluNI	TGGCCA	1 cut(s)	599
Mly113I	GGCGCC	1 cut(s)	103
MlyI	GAGTC	1 cut(s)	450
MnlI	CCTC	8 cut(s)	19, 60, 224, 463, 651, 760, 881, 915
Mox20I	TGGCCA	1 cut(s)	599
Mph1103I	ATGCAT	1 cut(s)	187
MroI	TCCGGA	1 cut(s)	226
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	5 cut(s)	83, 135, 177, 807, 864
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	2 cut(s)	501, 679
MspI	CCGG	1 cut(s)	227
MspR9I	CCNGG	4 cut(s)	108, 170, 352, 548
MvaI	CCWGG	4 cut(s)	108, 170, 352, 548
MwoI	GCNNNNNNNGC	5 cut(s)	197, 329, 532, 670, 901
NarI	GGCGCC	1 cut(s)	103
NdeII	GATC	3 cut(s)	139, 762, 783
NlaIII	CATG	2 cut(s)	189, 517
NlaIV	GGNNCC	5 cut(s)	104, 141, 474, 475, 772
NmeAIII	GCCGAG	1 cut(s)	910
NmuCI	GTSAC	1 cut(s)	509
NsiI	ATGCAT	1 cut(s)	187
NspI	RCATGY	1 cut(s)	189
PasI	CCCWGGG	1 cut(s)	107
PceI	AGGCCT	1 cut(s)	355
PfeI	GAWTC	2 cut(s)	50, 877
PflMI	CCANNNNNTGG	1 cut(s)	77
PfoI	TCCNGGA	1 cut(s)	546
PkrI	GCNGC	5 cut(s)	334, 525, 675, 678, 706
PleI	GAGTC	1 cut(s)	449
PluTI	GGCGCC	1 cut(s)	106
PpsI	GAGTC	1 cut(s)	449
PshAI	GACNNNNGTC	1 cut(s)	240
PshBI	ATTAAT	1 cut(s)	177
PsiI	TTATAA	1 cut(s)	419
Psp124BI	GAGCTC	1 cut(s)	639
Psp6I	CCWGG	4 cut(s)	106, 168, 350, 546
PspFI	CCCAGC	2 cut(s)	497, 758
PspGI	CCWGG	4 cut(s)	106, 168, 350, 546
PspN4I	GGNNCC	5 cut(s)	104, 141, 474, 475, 772
PspOMI	GGGCCC	1 cut(s)	473
PspPI	GGNCC	4 cut(s)	43, 242, 473, 474
PstI	CTGCAG	1 cut(s)	528
PstNI	CAGNNNCTG	1 cut(s)	679
PsuI	RGATCY	2 cut(s)	139, 783
PvuII	CAGCTG	1 cut(s)	679
SacI	GAGCTC	1 cut(s)	639
SaqAI	TTAA	5 cut(s)	83, 135, 177, 807, 864
SatI	GCNGC	5 cut(s)	333, 524, 674, 677, 705
Sau3AI	GATC	3 cut(s)	139, 762, 783
Sau96I	GGNCC	4 cut(s)	43, 242, 473, 474
SchI	GAGTC	1 cut(s)	450
ScrFI	CCNGG	4 cut(s)	108, 170, 352, 548
SduI	GDGCHC	3 cut(s)	477, 497, 639
SfaNI	GCATC	1 cut(s)	564
SfcI	CTRYAG	1 cut(s)	524
SfoI	GGCGCC	1 cut(s)	104
SinI	GGWCC	2 cut(s)	43, 242
SmlI	CTYRAG	2 cut(s)	638, 872
SmoI	CTYRAG	2 cut(s)	638, 872
Sse9I	AATT	5 cut(s)	84, 132, 151, 266, 803
SseBI	AGGCCT	1 cut(s)	355
SsiI	CCGC	2 cut(s)	124, 501
SspDI	GGCGCC	1 cut(s)	102
SstI	GAGCTC	1 cut(s)	639
StuI	AGGCCT	1 cut(s)	355
StyD4I	CCNGG	4 cut(s)	106, 168, 350, 546
StyI	CCWWGG	1 cut(s)	484
TaaI	ACNGT	3 cut(s)	224, 252, 492
TaiI	ACGT	1 cut(s)	234
TaqI	TCGA	3 cut(s)	254, 558, 765
TasI	AATT	5 cut(s)	84, 132, 151, 266, 803
TfiI	GAWTC	2 cut(s)	50, 877
Tru1I	TTAA	5 cut(s)	83, 135, 177, 807, 864
Tru9I	TTAA	5 cut(s)	83, 135, 177, 807, 864
TscAI	CASTG	2 cut(s)	78, 497
TseFI	GTSAC	1 cut(s)	509
TseI	GCWGC	5 cut(s)	332, 523, 673, 676, 704
Tsp45I	GTSAC	1 cut(s)	509
TspDTI	ATGAA	1 cut(s)	27
TspGWI	ACGGA	1 cut(s)	317
TspRI	CASTG	2 cut(s)	78, 497
Van91I	CCANNNNNTGG	1 cut(s)	77
VpaK11BI	GGWCC	2 cut(s)	43, 242
VspI	ATTAAT	1 cut(s)	177
XapI	RAATTY	2 cut(s)	266, 803
XceI	RCATGY	1 cut(s)	189
XmiI	GTMKAC	2 cut(s)	408, 626
Zsp2I	ATGCAT	1 cut(s)	187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.