pycom16g03720

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014725
Physical Location & Seq
Forward (+)
2309166 .. 2310273
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGTCAGGGAGAAACCGTATGTCTCGCCATTCTGATGGTTATCGAGTTTATCGTGATGTTCCGCGACCTGTTATGAACCGAGGACCAGGACCCTTGTCTATTCGCCCTGCAGCTCTGGAAGAAGAAGTTGCTGTGCAGCGTAGAGAAATGCAGCGAATTATTGCTGAGAACCAGCTTGTAATTGATGATAATACCCTTCTTCAAAGGCAATTGACAGATGCAAGAGATGAAATTCATAGATTGGGGCAGCTCATACCTAAAATCCGGGCTGAGAAAGAAGCGCAGTCTAGGGAGTTGATTGAGAGAGAATTGAAGCTAGAAGCTGATCTCCGTGCTACAGAGCCTTTAAAGGCGGAAGTTATGCAGTTAAGAGCTGAAGTTCATAAACTGAATAATTTACGGCAGGAACTGTCTTCCCAAGTCCAAGGTTTGACACAGGACGTTACTCGGTTTCAAGTGGAGAATCAGCAACTAATTGCCATGAGGGCTGATATTGATGTGATGCGAAACGAGCTTGTTGAGACCAGGAGAGCTTATGAATTTGAGAGGAAAGCCAATGAAGAACAAGTTGAGCAAAAGCAAGCAATGGAAAAGAATCTTGTCTCCATGGCTCGTGATATAGAGAAGCTAAGAGCAGAGCAGCTGAATGCAGACAGGAGGGCACGAGGACTAGGTGGTAAGAGTTACGGAATGATGAATGGAAGTCCCGATATGAGATATTCAGGTGGTCCATATCGCAATGGCTATAGCACCGGTTGGGGACCCTACGATAGGCGTGGATCACGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

264

Amino Acids

30.5

Weight (kDa)

9.44

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014725)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27400
malus_domestica MD13G1042300.v1.1 MD16G1043100.v1.1
prunus_persica Prupe.1G310800_v2.0.a1
pyrus_communis pycom13g03660 pycom13g03680 pycom16g03720
rosa_chinensis RchiOBHm_Chr4g0435211
rosa_laevigata RLG00000006609
rosa_multiflora Rmu_ssc0000339.1_g000029
rosa_roxburghii Rroxscaffold_5G00376420
rosa_rugosa Rorug04G0283100.1
rosa_samantha Rh4AG338800 Rh4BG346900 Rh4CG361500 Rh4DG341300
rosa_wichuraiana Rw4G029550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 64
AciI CCGC 2 cut(s) 62, 353
AclWI GGATC 1 cut(s) 787
AcsI RAATTY 2 cut(s) 231, 539
AcuI CTGAAG 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 771
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 3 cut(s) 203, 313, 455
AjnI CCWGG 2 cut(s) 85, 524
Alw26I GTCTC 3 cut(s) 27, 515, 607
AlwI GGATC 1 cut(s) 787
AlwNI CAGNNNCTG 1 cut(s) 409
ApeKI GCWGC 5 cut(s) 110, 136, 151, 247, 640
ApoI RAATTY 2 cut(s) 231, 539
AsiGI ACCGGT 1 cut(s) 752
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 4 cut(s) 83, 89, 728, 761
AsuC2I CCSGG 1 cut(s) 266
AvaII GGWCC 4 cut(s) 83, 89, 728, 761
BaeGI GKGCMC 1 cut(s) 664
BauI CACGAG 2 cut(s) 612, 663
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 5 cut(s) 122, 148, 163, 259, 652
BccI CCATC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 2 cut(s) 87, 526
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 3 cut(s) 27, 515, 607
BfaI CTAG 3 cut(s) 288, 317, 671
BfmI CTRYAG 3 cut(s) 108, 336, 745
BglI GCCNNNNNGGC 1 cut(s) 485
BisI GCNGC 5 cut(s) 111, 137, 152, 248, 641
BlsI GCNGC 5 cut(s) 112, 138, 153, 249, 642
Bme1390I CCNGG 3 cut(s) 87, 266, 526
Bme18I GGWCC 4 cut(s) 83, 89, 728, 761
BmgT120I GGNCC 4 cut(s) 83, 89, 728, 761
BmiI GGNNCC 3 cut(s) 91, 762, 763
BmrFI CCNGG 3 cut(s) 87, 266, 526
BmsI GCATC 2 cut(s) 208, 492
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 79, 424, 606
BsaWI WCCGGW 1 cut(s) 752
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 771
Bse118I RCCGGY 1 cut(s) 752
Bse3DI GCAATG 2 cut(s) 591, 745
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 2 cut(s) 87, 526
BseDI CCNNGG 3 cut(s) 79, 424, 606
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 771
BseMI GCAATG 2 cut(s) 591, 745
BseMII CTCAG 2 cut(s) 156, 261
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 5 cut(s) 122, 148, 163, 259, 652
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 64
BshTI ACCGGT 1 cut(s) 752
BsiSI CCGG 2 cut(s) 265, 753
BslFI GGGAC 2 cut(s) 690, 774
BslI CCNNNNNNNGG 1 cut(s) 771
BsmAI GTCTC 3 cut(s) 27, 515, 607
BsmFI GGGAC 2 cut(s) 690, 774
BsmI GAATGC 1 cut(s) 652
Bso31I GGTCTC 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 2 cut(s) 325, 779
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 2 cut(s) 62, 353
BspCNI CTCAG 2 cut(s) 157, 262
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 91, 762, 763
BspMAI CTGCAG 1 cut(s) 112
BspPI GGATC 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 515
BsrDI GCAATG 2 cut(s) 591, 745
BsrFI RCCGGY 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 79, 424, 606
BssMI GATC 2 cut(s) 325, 779
BssSI CACGAG 2 cut(s) 612, 663
BssT1I CCWWGG 2 cut(s) 424, 606
Bst2BI CACGAG 2 cut(s) 612, 663
Bst2UI CCWGG 2 cut(s) 87, 526
Bst4CI ACNGT 2 cut(s) 17, 411
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 3 cut(s) 165, 270, 629
BstDSI CCRYGG 1 cut(s) 606
BstENI CCTNNNNNAGG 1 cut(s) 769
BstFNI CGCG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 328, 782
BstMAI GTCTC 3 cut(s) 27, 515, 607
BstMBI GATC 2 cut(s) 325, 779
BstMWI GCNNNNNNNGC 2 cut(s) 485, 511
BstNI CCWGG 2 cut(s) 87, 526
BstPAI GACNNNNGTC 1 cut(s) 94
BstSCI CCNGG 3 cut(s) 85, 264, 524
BstSFI CTRYAG 3 cut(s) 108, 336, 745
BstSLI GKGCMC 1 cut(s) 664
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 5 cut(s) 122, 148, 163, 259, 652
BstV2I GAAGAC 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 606
Cac8I GCNNGC 1 cut(s) 582
CaiI CAGNNNCTG 1 cut(s) 409
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 4 cut(s) 83, 89, 728, 761
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 2 cut(s) 481, 607
DdeI CTNAG 3 cut(s) 165, 270, 629
DpnI GATC 2 cut(s) 327, 781
DpnII GATC 2 cut(s) 325, 779
DraI TTTAAA 1 cut(s) 348
EciI GGCGGA 1 cut(s) 368
Eco130I CCWWGG 2 cut(s) 424, 606
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 4 cut(s) 83, 89, 728, 761
Eco57I CTGAAG 1 cut(s) 396
EcoNI CCTNNNNNAGG 1 cut(s) 769
EcoO109I RGGNCCY 2 cut(s) 89, 761
EcoRII CCWGG 2 cut(s) 85, 524
EcoT14I CCWWGG 2 cut(s) 424, 606
ErhI CCWWGG 2 cut(s) 424, 606
FaeI CATG 2 cut(s) 484, 610
FaqI GGGAC 2 cut(s) 690, 774
FatI CATG 2 cut(s) 480, 606
Fnu4HI GCNGC 5 cut(s) 111, 137, 152, 248, 641
Fsp4HI GCNGC 5 cut(s) 111, 137, 152, 248, 641
FspBI CTAG 3 cut(s) 288, 317, 671
GlaI GCGC 1 cut(s) 282
GluI GCNGC 5 cut(s) 111, 137, 152, 248, 641
HapII CCGG 2 cut(s) 265, 753
HhaI GCGC 1 cut(s) 283
Hin1II CATG 2 cut(s) 484, 610
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 2 cut(s) 463, 595
HpaII CCGG 2 cut(s) 265, 753
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 5 cut(s) 53, 116, 614, 707, 783
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 17, 411
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 6 cut(s) 110, 136, 151, 221, 364, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 511
HpyF3I CTNAG 3 cut(s) 165, 270, 629
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 2 cut(s) 484, 610
HspAI GCGC 1 cut(s) 281
KflI GGGWCCC 1 cut(s) 761
Kzo9I GATC 2 cut(s) 325, 779
Lsp1109I GCAGC 5 cut(s) 122, 148, 163, 259, 652
LweI GCATC 2 cut(s) 208, 492
MaeI CTAG 3 cut(s) 288, 317, 671
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 442, 683
MalI GATC 2 cut(s) 327, 781
MboI GATC 2 cut(s) 325, 779
MboII GAAGA 5 cut(s) 131, 134, 191, 405, 572
MfeI CAATTG 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 664
MluCI AATT 8 cut(s) 156, 180, 209, 231, 308, 394, 474, 539
MnlI CCTC 5 cut(s) 74, 477, 540, 651, 659
MseI TTAA 3 cut(s) 347, 368, 790
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 2 cut(s) 265, 753
MspR9I CCNGG 3 cut(s) 87, 266, 526
MunI CAATTG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 87, 526
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 2 cut(s) 485, 511
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 606
NdeII GATC 2 cut(s) 325, 779
NlaIII CATG 2 cut(s) 484, 610
NlaIV GGNNCC 3 cut(s) 91, 762, 763
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 2 cut(s) 463, 595
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 5 cut(s) 112, 138, 153, 249, 642
PpuMI RGGWCCY 2 cut(s) 89, 761
PshAI GACNNNNGTC 1 cut(s) 94
Psp5II RGGWCCY 2 cut(s) 89, 761
Psp6I CCWGG 2 cut(s) 85, 524
PspGI CCWGG 2 cut(s) 85, 524
PspN4I GGNNCC 3 cut(s) 91, 762, 763
PspPI GGNCC 4 cut(s) 83, 89, 728, 761
PspPPI RGGWCCY 2 cut(s) 89, 761
PstI CTGCAG 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 643
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 3 cut(s) 347, 368, 790
SatI GCNGC 5 cut(s) 111, 137, 152, 248, 641
Sau3AI GATC 2 cut(s) 325, 779
Sau96I GGNCC 4 cut(s) 83, 89, 728, 761
ScrFI CCNGG 3 cut(s) 87, 266, 526
SduI GDGCHC 1 cut(s) 664
SfaNI GCATC 2 cut(s) 208, 492
SfcI CTRYAG 3 cut(s) 108, 336, 745
SinI GGWCC 4 cut(s) 83, 89, 728, 761
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 8 cut(s) 156, 180, 209, 231, 308, 394, 474, 539
SsiI CCGC 2 cut(s) 62, 353
SspMI CTAG 3 cut(s) 288, 317, 671
StyD4I CCNGG 3 cut(s) 85, 264, 524
StyI CCWWGG 2 cut(s) 424, 606
TaaI ACNGT 2 cut(s) 17, 411
TaiI ACGT 1 cut(s) 444
TaqI TCGA 1 cut(s) 43
TasI AATT 8 cut(s) 156, 180, 209, 231, 308, 394, 474, 539
TfiI GAWTC 2 cut(s) 463, 595
Tru1I TTAA 3 cut(s) 347, 368, 790
Tru9I TTAA 3 cut(s) 347, 368, 790
TseI GCWGC 5 cut(s) 110, 136, 151, 247, 640
TspDTI ATGAA 7 cut(s) 89, 224, 243, 371, 552, 573, 710
TspGWI ACGGA 2 cut(s) 320, 702
VpaK11BI GGWCC 4 cut(s) 83, 89, 728, 761
XagI CCTNNNNNAGG 1 cut(s) 769
XapI RAATTY 2 cut(s) 231, 539
XspI CTAG 3 cut(s) 288, 317, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.