pycom16g03920

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012396
Physical Location & Seq
Forward (+)
2426207 .. 2427672
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGGGAAGAAAGCTCGATGCTTTGCTTGGAAGGAGTCACAAGACCTCCGCCAAGTTCAAACCAGTTTTGAACCTCGCTCTCTCCCGCCTCGCCGTTCTCAAAAAACAGCGCCAGGTCAAGTGCTCTCAGGCTCGATCCGACGTCGTCCAGCTCCTCCAGCAGGGTCACCAAGATCGAGCTCTTCTTCGCGTGGAGCATGTGATTAAGGAGCAGAATATGTTGGACGTGTTTGTCATGATCGAGGGTTTTTGCAACCTCGTGATCGAAAGGGTTCATCTCATTGAACAAGAAAGGGAATGTCCTGATGAACTGAAGGAGGCAACATCGAGTTTGCTCTATGCGGCTTCAAGATGCGGGGATTTTCCGGAGCTTCAAGAGATTCGCGCGGTTCTCACTGCTCGTTTTGGCAAGGAATTTGCTGCTCGTGCCATCCAAGTGCGCAACAATTGTGGAGTCAACCTCACGATGATGCAAAAACTGTCAACTAGGATGCCAGTTTTGGAGGTCAGAATGAAGGTGCTCAAAGAAATAGCTGCAGAGAATAACATTGTTCTGACGCTGGAAGAGACGTCTTCTGTTTCCATTGAGGATAAATCGGATGCGAACAAGGCCAGTTCAAGTGGCACAGGACCAGCGGATAATGTGCAGGCTTCTGCACAAGAGACCGGAAGAGATGATAGGTTCTCTGGTTCGATTAAGCCAAGGAAATACAAGGATGTAGCTGATGCTGCGCAAGCAGCCTTCGAGTCTGCAGCTTATGCAGCAGATGCTGCAAAAGCAGCAGTGCGACTCTCTAGGTCTGAATCTCAAGATCCCGATCGATCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

277

Amino Acids

30.5

Weight (kDa)

8.91

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 20 - 183 1e-55 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012396)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13340
fragaria_vesca FvH4_4g27660
malus_domestica MD13G1043900.v1.1 MD16G1044700.v1.1
prunus_persica Prupe.1G308600_v2.0.a1
pyrus_communis pycom13g03850 pycom16g03920 pycom16g03930
rosa_chinensis RchiOBHm_Chr4g0435491
rosa_laevigata RLG00000006588
rosa_multiflora Rmu_co8134732.1_g000001 Rmu_sc0001940.1_g000028
rosa_roxburghii Rroxscaffold_5G00376720
rosa_rugosa Rorug04G0285600
rosa_samantha Rh4AG341300 Rh4BG349500 Rh4CG364200 Rh4DG343800
rosa_wichuraiana Rw4G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
AatII GACGTC 2 cut(s) 144, 572
Acc16I TGCGCA 2 cut(s) 440, 732
AccII CGCG 3 cut(s) 189, 384, 386
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 6 cut(s) 48, 85, 341, 354, 386, 635
AclWI GGATC 2 cut(s) 129, 806
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 2 cut(s) 141, 569
AfiI CCNNNNNNNGG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 6 cut(s) 58, 70, 284, 348, 374, 618
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 7 cut(s) 13, 151, 179, 370, 533, 722, 755
AluI AGCT 7 cut(s) 13, 151, 179, 370, 533, 722, 755
Alw21I GWGCWC 3 cut(s) 125, 181, 522
Alw26I GTCTC 2 cut(s) 560, 656
AlwI GGATC 2 cut(s) 129, 806
AlwNI CAGNNNCTG 1 cut(s) 770
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 8 cut(s) 419, 533, 728, 737, 752, 761, 770, 779
ApoI RAATTY 1 cut(s) 413
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 4 cut(s) 111, 386, 441, 733
AspS9I GGNCC 1 cut(s) 629
AsuHPI GGTGA 1 cut(s) 158
AvaII GGWCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 181
BauI CACGAG 2 cut(s) 257, 423
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 3 cut(s) 125, 181, 522
BbvI GCAGC 8 cut(s) 406, 520, 715, 749, 757, 764, 773, 791
BccI CCATC 1 cut(s) 437
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 560, 656
BfaI CTAG 2 cut(s) 486, 795
BfmI CTRYAG 2 cut(s) 534, 750
BfoI RGCGCY 1 cut(s) 112
BisI GCNGC 9 cut(s) 342, 420, 534, 729, 738, 753, 762, 771, 780
BlsI GCNGC 9 cut(s) 343, 421, 535, 730, 739, 754, 763, 772, 781
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 629
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 629
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 7 cut(s) 7, 341, 459, 480, 589, 715, 757
BpiI GAAGAC 1 cut(s) 564
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 792
Bsa29I ATCGAT 1 cut(s) 820
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 2 cut(s) 141, 569
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 2 cut(s) 364, 665
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 3 cut(s) 62, 494, 612
Bse8I GATNNNNATC 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 1 cut(s) 113
BseCI ATCGAT 1 cut(s) 820
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 4 cut(s) 429, 495, 604, 721
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 62, 494, 612
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 8 cut(s) 406, 520, 715, 749, 757, 764, 773, 791
BsgI GTGCAG 2 cut(s) 639, 665
Bsh1236I CGCG 3 cut(s) 189, 384, 386
Bsh1285I CGRYCG 1 cut(s) 820
BshFI GGCC 1 cut(s) 611
BshVI ATCGAT 1 cut(s) 820
BsiEI CGRYCG 1 cut(s) 820
BsiHKAI GWGCWC 3 cut(s) 125, 181, 522
BsiSI CCGG 2 cut(s) 365, 666
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 560, 656
BsmBI CGTCTC 1 cut(s) 560
BsnI GGCC 1 cut(s) 611
Bso31I GGTCTC 1 cut(s) 656
Bsp1286I GDGCHC 3 cut(s) 125, 181, 522
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
BspACI CCGC 6 cut(s) 48, 85, 341, 354, 386, 635
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 820
BspEI TCCGGA 1 cut(s) 364
BspFNI CGCG 3 cut(s) 189, 384, 386
BspHI TCATGA 1 cut(s) 234
BspMAI CTGCAG 2 cut(s) 538, 754
BspPI GGATC 2 cut(s) 129, 806
BspQI GCTCTTC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 656
BsrI ACTGG 3 cut(s) 62, 494, 612
BssECI CCNNGG 1 cut(s) 701
BssMI GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
BssNI GRCGYC 2 cut(s) 141, 569
BssSI CACGAG 2 cut(s) 257, 423
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 2 cut(s) 257, 423
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 3 cut(s) 186, 558, 664
BstACI GRCGYC 2 cut(s) 141, 569
BstAPI GCANNNNNTGC 3 cut(s) 758, 767, 770
BstC8I GCNNGC 2 cut(s) 648, 735
BstDEI CTNAG 1 cut(s) 126
BstEII GGTNACC 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 429, 495, 604, 721
BstFNI CGCG 3 cut(s) 189, 384, 386
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 4 cut(s) 111, 386, 441, 733
BstKTI GATC 7 cut(s) 137, 175, 240, 264, 814, 820, 824
BstMAI GTCTC 2 cut(s) 560, 656
BstMBI GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
BstMCI CGRYCG 1 cut(s) 820
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 200
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 534, 750
BstUI CGCG 3 cut(s) 189, 384, 386
BstV1I GCAGC 8 cut(s) 406, 520, 715, 749, 757, 764, 773, 791
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 820
BsuRI GGCC 1 cut(s) 611
BsuTUI ATCGAT 1 cut(s) 820
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 4 cut(s) 429, 495, 604, 721
BtsI GCAGTG 2 cut(s) 393, 789
BtsIMutI CAGTG 2 cut(s) 393, 789
Cac8I GCNNGC 2 cut(s) 648, 735
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 1 cut(s) 234
CfoI GCGC 4 cut(s) 111, 386, 441, 733
Cfr13I GGNCC 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 820
CseI GACGC 1 cut(s) 565
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 2 cut(s) 197, 235
DdeI CTNAG 1 cut(s) 126
DpnI GATC 7 cut(s) 136, 174, 239, 263, 813, 819, 823
DpnII GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 186, 558, 664
EarI CTCTTC 3 cut(s) 186, 558, 664
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 701
Eco24I GRGCYC 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 701
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 701
Esp3I CGTCTC 1 cut(s) 560
FaeI CATG 2 cut(s) 200, 238
FaiI YATR 5 cut(s) 198, 218, 236, 339, 759
FatI CATG 2 cut(s) 196, 234
FauI CCCGC 2 cut(s) 92, 347
Fnu4HI GCNGC 9 cut(s) 342, 420, 534, 729, 738, 753, 762, 771, 780
FokI GGATG 4 cut(s) 416, 502, 611, 728
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 9 cut(s) 342, 420, 534, 729, 738, 753, 762, 771, 780
FspBI CTAG 2 cut(s) 486, 795
FspI TGCGCA 2 cut(s) 440, 732
GlaI GCGC 4 cut(s) 110, 385, 440, 732
GluI GCNGC 9 cut(s) 342, 420, 534, 729, 738, 753, 762, 771, 780
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 1 cut(s) 611
HapII CCGG 2 cut(s) 365, 666
HgaI GACGC 1 cut(s) 565
HhaI GCGC 4 cut(s) 111, 386, 441, 733
Hin1I GRCGYC 2 cut(s) 141, 569
Hin1II CATG 2 cut(s) 200, 238
Hin6I GCGC 4 cut(s) 109, 384, 439, 731
HinP1I GCGC 4 cut(s) 109, 384, 439, 731
HincII GTYRAC 2 cut(s) 457, 483
HindII GTYRAC 2 cut(s) 457, 483
HinfI GANTC 6 cut(s) 34, 379, 453, 746, 789, 803
HpaII CCGG 2 cut(s) 365, 666
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 457, 483
Hpy188I TCNGA 5 cut(s) 139, 509, 555, 598, 802
Hpy188III TCNNGA 9 cut(s) 235, 259, 302, 348, 365, 374, 463, 809, 815
Hpy8I GTNNAC 2 cut(s) 457, 483
Hpy99I CGWCG 2 cut(s) 143, 146
HpyAV CCTTC 4 cut(s) 24, 307, 508, 751
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 3 cut(s) 141, 225, 569
HpyCH4V TGCA 8 cut(s) 252, 472, 536, 646, 656, 752, 761, 773
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 3 cut(s) 141, 225, 569
Hsp92I GRCGYC 2 cut(s) 141, 569
Hsp92II CATG 2 cut(s) 200, 238
HspAI GCGC 4 cut(s) 109, 384, 439, 731
Kpn2I TCCGGA 1 cut(s) 364
Kzo9I GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 4 cut(s) 156, 193, 208, 367
Lsp1109I GCAGC 8 cut(s) 406, 520, 715, 749, 757, 764, 773, 791
LweI GCATC 7 cut(s) 7, 341, 459, 480, 589, 715, 757
MaeI CTAG 2 cut(s) 486, 795
MaeII ACGT 3 cut(s) 141, 225, 569
MaeIII GTNAC 2 cut(s) 35, 164
MalI GATC 7 cut(s) 136, 174, 239, 263, 813, 819, 823
MboI GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
MboII GAAGA 6 cut(s) 18, 173, 176, 564, 575, 681
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 3 cut(s) 125, 181, 522
MluCI AATT 2 cut(s) 413, 445
MlyI GAGTC 4 cut(s) 43, 462, 755, 783
MmeI TCCRAC 2 cut(s) 162, 201
MroI TCCGGA 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 2 cut(s) 204, 696
MslI CAYNNNNRTG 2 cut(s) 434, 467
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 2 cut(s) 365, 666
MspR9I CCNGG 1 cut(s) 113
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 3 cut(s) 189, 384, 386
NdeII GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
NlaIII CATG 2 cut(s) 200, 238
NmuCI GTSAC 2 cut(s) 35, 164
NsbI TGCGCA 2 cut(s) 440, 732
NspI RCATGY 1 cut(s) 200
PagI TCATGA 1 cut(s) 234
PciSI GCTCTTC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 379, 803
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 9 cut(s) 343, 421, 535, 730, 739, 754, 763, 772, 781
Ple19I CGATCG 1 cut(s) 820
PleI GAGTC 4 cut(s) 42, 461, 754, 783
PpsI GAGTC 4 cut(s) 42, 461, 754, 783
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 111
PspPI GGNCC 1 cut(s) 629
PstI CTGCAG 2 cut(s) 538, 754
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 1 cut(s) 811
PsyI GACNNNGTC 1 cut(s) 143
PvuI CGATCG 1 cut(s) 820
RseI CAYNNNNRTG 2 cut(s) 434, 467
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 204, 696
SatI GCNGC 9 cut(s) 342, 420, 534, 729, 738, 753, 762, 771, 780
Sau3AI GATC 7 cut(s) 134, 172, 237, 261, 811, 817, 821
Sau96I GGNCC 1 cut(s) 629
SchI GAGTC 4 cut(s) 43, 462, 755, 783
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 3 cut(s) 125, 181, 522
SfaNI GCATC 7 cut(s) 7, 341, 459, 480, 589, 715, 757
SfcI CTRYAG 2 cut(s) 534, 750
SinI GGWCC 1 cut(s) 629
SmiMI CAYNNNNRTG 2 cut(s) 434, 467
SmlI CTYRAG 1 cut(s) 807
SmoI CTYRAG 1 cut(s) 807
Sse9I AATT 2 cut(s) 413, 445
SsiI CCGC 6 cut(s) 48, 85, 341, 354, 386, 635
SspMI CTAG 2 cut(s) 486, 795
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 3 cut(s) 144, 228, 572
TaqI TCGA 9 cut(s) 15, 133, 175, 240, 264, 326, 692, 744, 820
TasI AATT 2 cut(s) 413, 445
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 379, 803
Tru1I TTAA 2 cut(s) 204, 696
Tru9I TTAA 2 cut(s) 204, 696
TscAI CASTG 2 cut(s) 400, 789
TseFI GTSAC 2 cut(s) 35, 164
TseI GCWGC 8 cut(s) 419, 533, 728, 737, 752, 761, 770, 779
Tsp45I GTSAC 2 cut(s) 35, 164
TspDTI ATGAA 3 cut(s) 263, 321, 527
TspRI CASTG 2 cut(s) 400, 789
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 629
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 413
XceI RCATGY 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 2 cut(s) 486, 795
ZraI GACGTC 2 cut(s) 142, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.