pycom16g04770

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007725
Physical Location & Seq
Forward (+)
3061510 .. 3064022
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGTACGTTATTTATTTCTATTTATTTCCTTACATCTTCTCTGCAAGTAACATGTCTAAAGCAAATATTATAAGAAGACATGTACTTCTGGAGAAGAACAAAGTTGTAAAGGAGAGGGAGAAGACGAACAGTAACCCTAAATACCTCAAGCGAATTTACCCAATTGGCCTTCACAAGAGCACTTCATCTCTATCCCTATCTTCATCATCATCTCTGTCATTCTCCTTGTCGGAGAACTCGTATGATTCTTCTCTCACCGATTCTACCTTTCCACTGGATCAGAAGATTTCCGCGGCGTTCCGCTTCATTGCACCACCACCTCCAAGAAGAGAGTATAATTCTCCGGTGGCTAAACTTGCCCAGCAGCAGATTAGTCTGGCTCAGGATGCTAATGATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAATAGTGATAAAGTTTATGTAGCGTTTCATGACGAATGCTGGGGAGTTCCAGCATACGATGACAATCAATTGTTTGAGCTGCTTGCACTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTTTTCAGGGAAGCGTTTTCTGGATTCGATCCGAACAAAGTTGCAAAAATGGAGGAGAAAGAGATTGCGGAAATAACTTCCAACAAAGAAATAATGCTGGTAGACTGCAGAGTGAGGTGCATTATAAACAATGCCAAATGCATATTGAAGATTGTGAGGGAGTTTGGATCTTTCAGCAGCTACATTTGGAGTTATGTGAATCACAAACCAGTCATAAACCGATTCAGATACCCCAGAAACGTTCCTCTCAGGACTCCGAAAGCAGAATCCATGAGCAAGGACTTGATCAAGAGAGGGTTTCGATACGTTGGACCGGTGATCGTGTACTCGTTCATGCAGGCTGCAGGGTTGACAAATGATCATCTGGTGGATTGTTATAGGTACAGTGAATGTGTGAACCTTGCTGAAAGACCTTGGAGACATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

333

Amino Acids

38.54

Weight (kDa)

9.28

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 143 - 317 1.5e-68 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 71, 695
AccI GTMKAC 1 cut(s) 672
AccII CGCG 1 cut(s) 293
AciI CCGC 4 cut(s) 291, 293, 301, 638
AclI AACGTT 1 cut(s) 810
AclWI GGATC 3 cut(s) 285, 593, 745
AcsI RAATTY 1 cut(s) 153
AfaI GTAC 4 cut(s) 5, 84, 896, 953
AflIII ACRYGT 2 cut(s) 51, 79
AgeI ACCGGT 1 cut(s) 883
AgsI TTSAA 1 cut(s) 718
AluBI AGCT 3 cut(s) 508, 571, 750
AluI AGCT 3 cut(s) 508, 571, 750
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 982
AlwI GGATC 3 cut(s) 285, 593, 745
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 4 cut(s) 364, 508, 747, 911
ApoI RAATTY 1 cut(s) 153
AsiGI ACCGGT 1 cut(s) 883
AspS9I GGNCC 2 cut(s) 535, 881
AsuHPI GGTGA 2 cut(s) 247, 898
AvaII GGWCC 2 cut(s) 535, 881
BbsI GAAGAC 2 cut(s) 82, 128
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 4 cut(s) 376, 495, 759, 898
BccI CCATC 2 cut(s) 389, 526
BclI TGATCA 2 cut(s) 855, 928
BcoDI GTCTC 1 cut(s) 982
BfmI CTRYAG 2 cut(s) 676, 912
BisI GCNGC 5 cut(s) 294, 365, 509, 748, 912
BlsI GCNGC 5 cut(s) 295, 366, 510, 749, 913
Bme18I GGWCC 2 cut(s) 535, 881
BmgT120I GGNCC 2 cut(s) 535, 881
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 2 cut(s) 82, 128
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 131
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 291, 983
BsaWI WCCGGW 2 cut(s) 343, 883
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bse118I RCCGGY 1 cut(s) 883
Bse1I ACTGG 2 cut(s) 279, 779
Bse3DI GCAATG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 291, 983
BseGI GGATG 1 cut(s) 391
BseJI GATNNNNATC 1 cut(s) 492
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 395, 832
BseNI ACTGG 2 cut(s) 279, 779
BseRI GAGGAG 2 cut(s) 421, 638
BseXI GCAGC 4 cut(s) 376, 495, 759, 898
BseYI CCCAGC 2 cut(s) 360, 468
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 1 cut(s) 168
BshTI ACCGGT 1 cut(s) 883
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 2 cut(s) 344, 884
BsmAI GTCTC 1 cut(s) 982
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 6 cut(s) 277, 598, 737, 855, 888, 928
BspACI CCGC 4 cut(s) 291, 293, 301, 638
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 394, 831
BspFNI CGCG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 457
BspMAI CTGCAG 2 cut(s) 680, 916
BspPI GGATC 3 cut(s) 285, 593, 745
BsrDI GCAATG 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 883
BsrI ACTGG 2 cut(s) 279, 779
BssAI RCCGGY 1 cut(s) 883
BssECI CCNNGG 2 cut(s) 291, 983
BssMI GATC 6 cut(s) 277, 598, 737, 855, 888, 928
BssT1I CCWWGG 1 cut(s) 983
Bst4CI ACNGT 3 cut(s) 131, 519, 956
Bst6I CTCTTC 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 513, 909
BstDEI CTNAG 2 cut(s) 381, 818
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 6 cut(s) 280, 601, 740, 858, 891, 931
BstMAI GTCTC 1 cut(s) 982
BstMBI GATC 6 cut(s) 277, 598, 737, 855, 888, 928
BstMWI GCNNNNNNNGC 2 cut(s) 356, 386
BstNSI RCATGY 2 cut(s) 55, 83
BstSFI CTRYAG 2 cut(s) 676, 912
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 4 cut(s) 376, 495, 759, 898
BstV2I GAAGAC 2 cut(s) 82, 128
BstX2I RGATCY 1 cut(s) 737
BstYI RGATCY 1 cut(s) 737
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 3 cut(s) 272, 515, 961
Cac8I GCNNGC 2 cut(s) 513, 909
CciI TCATGA 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 883
Cfr13I GGNCC 2 cut(s) 535, 881
Cfr42I CCGCGG 1 cut(s) 294
Csp6I GTAC 4 cut(s) 4, 83, 895, 952
CspAI ACCGGT 1 cut(s) 883
CviAII CATG 5 cut(s) 52, 80, 458, 841, 904
CviJI RGCY 7 cut(s) 168, 350, 380, 508, 571, 750, 911
CviKI_1 RGCY 7 cut(s) 168, 350, 380, 508, 571, 750, 911
CviQI GTAC 4 cut(s) 4, 83, 895, 952
DdeI CTNAG 2 cut(s) 381, 818
DpnI GATC 6 cut(s) 279, 600, 739, 857, 890, 930
DpnII GATC 6 cut(s) 277, 598, 737, 855, 888, 928
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 983
Eco47I GGWCC 2 cut(s) 535, 881
EcoT14I CCWWGG 1 cut(s) 983
EcoT22I ATGCAT 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 983
FaeI CATG 5 cut(s) 55, 83, 461, 844, 907
FatI CATG 5 cut(s) 51, 79, 457, 840, 903
FbaI TGATCA 2 cut(s) 855, 928
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 294, 365, 509, 748, 912
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 5 cut(s) 294, 365, 509, 748, 912
GluI GCNGC 5 cut(s) 294, 365, 509, 748, 912
GsaI CCCAGC 2 cut(s) 364, 472
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 168
HapII CCGG 2 cut(s) 344, 884
Hin1II CATG 5 cut(s) 55, 83, 461, 844, 907
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HinfI GANTC 7 cut(s) 245, 260, 594, 769, 792, 823, 836
HpaII CCGG 2 cut(s) 344, 884
HphI GGTGA 2 cut(s) 247, 898
Hpy166II GTNNAC 4 cut(s) 673, 895, 921, 967
Hpy188I TCNGA 5 cut(s) 232, 282, 603, 797, 828
Hpy188III TCNNGA 6 cut(s) 89, 383, 458, 591, 820, 859
Hpy8I GTNNAC 4 cut(s) 673, 895, 921, 967
HpyAV CCTTC 2 cut(s) 179, 558
HpyCH4III ACNGT 3 cut(s) 131, 519, 956
HpyCH4IV ACGT 3 cut(s) 6, 810, 876
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 386
HpyF3I CTNAG 2 cut(s) 381, 818
HpySE526I ACGT 3 cut(s) 6, 810, 876
Hsp92II CATG 5 cut(s) 55, 83, 461, 844, 907
Ksp22I TGATCA 2 cut(s) 855, 928
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 6 cut(s) 277, 598, 737, 855, 888, 928
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 4 cut(s) 376, 495, 759, 898
LweI GCATC 1 cut(s) 376
MaeII ACGT 3 cut(s) 6, 810, 876
MaeIII GTNAC 2 cut(s) 47, 131
MalI GATC 6 cut(s) 279, 600, 739, 857, 890, 930
MboI GATC 6 cut(s) 277, 598, 737, 855, 888, 928
MboII GAAGA 9 cut(s) 28, 87, 106, 133, 192, 240, 295, 339, 730
MfeI CAATTG 4 cut(s) 162, 415, 497, 541
MflI RGATCY 1 cut(s) 737
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 7 cut(s) 153, 162, 337, 415, 497, 541, 552
MlyI GAGTC 1 cut(s) 817
MmeI TCCRAC 3 cut(s) 210, 675, 859
Mph1103I ATGCAT 1 cut(s) 713
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 344, 884
MunI CAATTG 4 cut(s) 162, 415, 497, 541
Mva1269I GAATGC 1 cut(s) 470
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 356, 386
NdeII GATC 6 cut(s) 277, 598, 737, 855, 888, 928
NlaIII CATG 5 cut(s) 55, 83, 461, 844, 907
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 2 cut(s) 55, 83
PagI TCATGA 1 cut(s) 457
PciI ACATGT 2 cut(s) 51, 79
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 6 cut(s) 245, 260, 594, 769, 792, 836
PinAI ACCGGT 1 cut(s) 883
PkrI GCNGC 5 cut(s) 295, 366, 510, 749, 913
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
PscI ACATGT 2 cut(s) 51, 79
PsiI TTATAA 2 cut(s) 71, 695
Psp1406I AACGTT 1 cut(s) 810
PspFI CCCAGC 2 cut(s) 360, 468
PspPI GGNCC 2 cut(s) 535, 881
PstI CTGCAG 2 cut(s) 680, 916
PsuI RGATCY 1 cut(s) 737
RsaI GTAC 4 cut(s) 5, 84, 896, 953
RsaNI GTAC 4 cut(s) 4, 83, 895, 952
SacII CCGCGG 1 cut(s) 294
SatI GCNGC 5 cut(s) 294, 365, 509, 748, 912
Sau3AI GATC 6 cut(s) 277, 598, 737, 855, 888, 928
Sau96I GGNCC 2 cut(s) 535, 881
SchI GAGTC 1 cut(s) 817
SduI GDGCHC 1 cut(s) 182
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 676, 912
Sfr303I CCGCGG 1 cut(s) 294
SgrBI CCGCGG 1 cut(s) 294
SinI GGWCC 2 cut(s) 535, 881
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 7 cut(s) 153, 162, 337, 415, 497, 541, 552
SsiI CCGC 4 cut(s) 291, 293, 301, 638
SspI AATATT 1 cut(s) 67
StyI CCWWGG 1 cut(s) 983
TaaI ACNGT 3 cut(s) 131, 519, 956
TaiI ACGT 3 cut(s) 9, 813, 879
TaqI TCGA 2 cut(s) 597, 871
TasI AATT 7 cut(s) 153, 162, 337, 415, 497, 541, 552
TatI WGTACW 2 cut(s) 82, 894
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 6 cut(s) 245, 260, 594, 769, 792, 836
TscAI CASTG 3 cut(s) 279, 522, 961
TseI GCWGC 4 cut(s) 364, 508, 747, 911
TspDTI ATGAA 5 cut(s) 174, 192, 295, 446, 892
TspRI CASTG 3 cut(s) 279, 522, 961
VpaK11BI GGWCC 2 cut(s) 535, 881
XapI RAATTY 1 cut(s) 153
XceI RCATGY 2 cut(s) 55, 83
XmiI GTMKAC 1 cut(s) 672
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.