pycom16g05190

6-phosphogluconolactonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006227
Physical Location & Seq
Reverse (-)
3375830 .. 3378888
3059 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 945 bp
ATGAAATACTATAAATATATTATATATAAACAAATTGGTATAGATTCCCATCTTCCACCTTCGTCAGTTTCTTGCTTCTCTGCCACCTCTCCCTCCTCCTTCCTTCTTCTTCCTCCCCCTCTTGGTGTTGAACTTGTTGGAAGCATGGCAATTTCCGGGGTTCGAAAAGATAGAGAGTTGAGGATTCATGAAAGTTTGGATGAGCTGAGCACTGATTTGGCAGACTACATTGCTGAGCTATCAGAGGCATCCGTGAAGGAGCGCGGCGTCTTTGCAATTGCATTATCTGGTGGTTCTCTCATTGGCTTAATGGGGAAACTGTGTGAAGCTCCTTATAACAAGACTGTAGATTGGGCCAAATGGTATATATTTTGGGCAGACGAGCGTGTTGTGGCGAAAAGTCATGTCGATAGCAACTACAAGCTTGCGAAAGATCGCCTTTTGTCCAAGGTACCTATAATTCCCAGTCATGTGCATTCTATTAATGATTCAGTGTCAGCAGAGGAGGCTGCTGATGACTATGAGTTTGTCATTCGCCAGTTAGTGAAATCTCGTGTGATCAGCGTGTCTGATATAAGTGACTGCCCCAAGTTTGACCTAATCCTCCTGGGAATGGGTCCTGATGGGCACGTCGCCTCACTATTCCCTAACCACTCGGTGCTGGAGAAGAAAGATGAATGGGTAACTTTTATAACTGACTCCCCCAAACCTCCACCCGAGAGAATCACGTTCACTCTGCCAGTCATCAACTCGGCATCCAATGTGGCGATAGTCGCAACAGGTGACAGCAAAGCAGAGGCTGCGCACATGGCAGTCGACGACATGGGATCTGACCTCCCTCCAGTGCCGGCTGCAATTGTCCAGCCACTGAAAGGGAAGTTGGTGTGGTTTTTGGACAAGGCAGCTGCCTCAAAACTTGATGACGGTACTCAAGTCTCCGCATAG

Protein Analysis

315

Amino Acids

34.17

Weight (kDa)

5.29

Isoelectric Point (pI)

30.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucosamine_iso PF01182 66 - 296 2.3e-73 Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 336, 692
Acc16I TGCGCA 1 cut(s) 804
Acc65I GGTACC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 816
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 264, 939
AclWI GGATC 1 cut(s) 835
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 2 cut(s) 453, 928
AfiI CCNNNNNNNGG 3 cut(s) 122, 613, 872
AgsI TTSAA 1 cut(s) 131
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 1 cut(s) 606
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 5 cut(s) 205, 238, 329, 424, 905
AluI AGCT 5 cut(s) 205, 238, 329, 424, 905
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 940
AlwI GGATC 1 cut(s) 835
AlwNI CAGNNNCTG 2 cut(s) 800, 868
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 5 cut(s) 509, 800, 851, 902, 905
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AseI ATTAAT 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 451
AspLEI GCGC 2 cut(s) 264, 805
AspS9I GGNCC 2 cut(s) 354, 617
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 794
AsuII TTCGAA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 630
BaeI ACNNNNGTAYC 1 cut(s) 918
BanI GGYRCC 1 cut(s) 451
BauI CACGAG 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 5 cut(s) 496, 787, 838, 892, 914
BccI CCATC 2 cut(s) 57, 617
BciT130I CCWGG 1 cut(s) 608
BclI TGATCA 1 cut(s) 558
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 940
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 6 cut(s) 265, 510, 801, 852, 903, 906
BlpI GCTNAGC 2 cut(s) 206, 234
BlsI GCNGC 6 cut(s) 266, 511, 802, 853, 904, 907
Bme1390I CCNGG 2 cut(s) 157, 608
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 716
BmgBI CACGTC 1 cut(s) 631
BmgT120I GGNCC 2 cut(s) 354, 617
BmiI GGNNCC 2 cut(s) 453, 618
BmrFI CCNGG 2 cut(s) 157, 608
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 257, 764
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 2 cut(s) 683, 825
Bpu1102I GCTNAGC 2 cut(s) 206, 234
Bpu14I TTCGAA 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 915
BpuMI CCSGG 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 48
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 156, 447, 607
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 613, 872
Bse118I RCCGGY 1 cut(s) 847
Bse1I ACTGG 4 cut(s) 465, 538, 740, 842
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 3 cut(s) 156, 447, 607
BseGI GGATG 3 cut(s) 205, 248, 755
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 3 cut(s) 122, 613, 872
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 197, 225
BseNI ACTGG 4 cut(s) 465, 538, 740, 842
BseRI GAGGAG 2 cut(s) 85, 518
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 5 cut(s) 496, 787, 838, 892, 914
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 356
BshNI GGYRCC 1 cut(s) 451
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 2 cut(s) 156, 848
BslI CCNNNNNNNGG 3 cut(s) 122, 613, 872
BsmAI GTCTC 1 cut(s) 940
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 716
Bsp119I TTCGAA 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 212, 630
Bsp143I GATC 3 cut(s) 433, 558, 827
Bsp1720I GCTNAGC 2 cut(s) 206, 234
BspACI CCGC 2 cut(s) 264, 939
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 2 cut(s) 198, 226
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 453, 618
BspPI GGATC 1 cut(s) 835
BspT104I TTCGAA 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 847
BsrI ACTGG 4 cut(s) 465, 538, 740, 842
BssAI RCCGGY 1 cut(s) 847
BssECI CCNNGG 3 cut(s) 156, 447, 607
BssMI GATC 3 cut(s) 433, 558, 827
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 447
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 3 cut(s) 321, 346, 926
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 426, 849
BstDEI CTNAG 2 cut(s) 206, 234
BstF5I GGATG 3 cut(s) 205, 248, 755
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 2 cut(s) 264, 805
BstKTI GATC 3 cut(s) 436, 561, 830
BstMAI GTCTC 1 cut(s) 940
BstMBI GATC 3 cut(s) 433, 558, 827
BstMWI GCNNNNNNNGC 4 cut(s) 506, 773, 800, 809
BstNI CCWGG 1 cut(s) 608
BstSCI CCNGG 2 cut(s) 155, 606
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 630
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 5 cut(s) 496, 787, 838, 892, 914
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
BsuRI GGCC 1 cut(s) 356
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 3 cut(s) 205, 248, 755
BtsIMutI CAGTG 4 cut(s) 210, 498, 849, 866
Cac8I GCNNGC 2 cut(s) 426, 849
CaiI CAGNNNCTG 2 cut(s) 800, 868
CciI TCATGA 1 cut(s) 187
CfoI GCGC 2 cut(s) 264, 805
Cfr10I RCCGGY 1 cut(s) 847
Cfr13I GGNCC 2 cut(s) 354, 617
CseI GACGC 1 cut(s) 256
Csp6I GTAC 2 cut(s) 452, 927
CviAII CATG 6 cut(s) 145, 188, 404, 470, 808, 823
CviQI GTAC 2 cut(s) 452, 927
DdeI CTNAG 2 cut(s) 206, 234
DpnI GATC 3 cut(s) 435, 560, 829
DpnII GATC 3 cut(s) 433, 558, 827
DraIII CACNNNGTG 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 716
EcoO109I RGGNCCY 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 6 cut(s) 148, 191, 407, 473, 811, 826
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FatI CATG 6 cut(s) 144, 187, 403, 469, 807, 822
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 6 cut(s) 265, 510, 801, 852, 903, 906
FokI GGATG 3 cut(s) 212, 235, 742
Fsp4HI GCNGC 6 cut(s) 265, 510, 801, 852, 903, 906
FspI TGCGCA 1 cut(s) 804
GlaI GCGC 2 cut(s) 263, 804
GluI GCNGC 6 cut(s) 265, 510, 801, 852, 903, 906
GsuI CTGGAG 2 cut(s) 683, 825
HaeIII GGCC 1 cut(s) 356
HapII CCGG 2 cut(s) 156, 848
HgaI GACGC 1 cut(s) 256
HhaI GCGC 2 cut(s) 264, 805
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 148, 191, 407, 473, 811, 826
Hin6I GCGC 2 cut(s) 262, 803
HinP1I GCGC 2 cut(s) 262, 803
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 5 cut(s) 44, 184, 488, 698, 723
HpaII CCGG 2 cut(s) 156, 848
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 2 cut(s) 732, 817
Hpy188I TCNGA 3 cut(s) 244, 571, 832
Hpy188III TCNNGA 2 cut(s) 188, 620
Hpy8I GTNNAC 2 cut(s) 732, 817
Hpy99I CGWCG 2 cut(s) 635, 821
HpyAV CCTTC 4 cut(s) 69, 109, 113, 250
HpyCH4III ACNGT 3 cut(s) 321, 346, 926
HpyCH4IV ACGT 2 cut(s) 630, 728
HpyCH4V TGCA 4 cut(s) 275, 281, 475, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 773, 800, 809
HpyF3I CTNAG 2 cut(s) 206, 234
HpySE526I ACGT 2 cut(s) 630, 728
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 148, 191, 407, 473, 811, 826
HspAI GCGC 2 cut(s) 262, 803
KpnI GGTACC 1 cut(s) 455
KroI GCCGGC 1 cut(s) 847
KroNI GCCGGC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 3 cut(s) 433, 558, 827
LmnI GCTCC 2 cut(s) 259, 334
Lsp1109I GCAGC 5 cut(s) 496, 787, 838, 892, 914
LweI GCATC 2 cut(s) 257, 764
MaeII ACGT 2 cut(s) 630, 728
MaeIII GTNAC 3 cut(s) 578, 682, 782
MalI GATC 3 cut(s) 435, 560, 829
MboI GATC 3 cut(s) 433, 558, 827
MboII GAAGA 4 cut(s) 44, 98, 101, 679
MfeI CAATTG 2 cut(s) 276, 855
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 2 cut(s) 212, 630
MluCI AATT 5 cut(s) 33, 150, 276, 459, 855
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 847
MseI TTAA 2 cut(s) 308, 483
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 2 cut(s) 156, 848
MspR9I CCNGG 2 cut(s) 157, 608
MunI CAATTG 2 cut(s) 276, 855
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 608
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 4 cut(s) 506, 773, 800, 809
NaeI GCCGGC 1 cut(s) 849
NciI CCSGG 1 cut(s) 157
NdeII GATC 3 cut(s) 433, 558, 827
NgoMIV GCCGGC 1 cut(s) 847
NlaIII CATG 6 cut(s) 148, 191, 407, 473, 811, 826
NlaIV GGNNCC 2 cut(s) 453, 618
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 2 cut(s) 578, 782
NsbI TGCGCA 1 cut(s) 804
NspV TTCGAA 1 cut(s) 163
PagI TCATGA 1 cut(s) 187
PctI GAATGC 1 cut(s) 475
PdiI GCCGGC 1 cut(s) 849
PfeI GAWTC 4 cut(s) 44, 184, 488, 723
PkrI GCNGC 6 cut(s) 266, 511, 802, 853, 904, 907
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 617
PshBI ATTAAT 1 cut(s) 483
PsiI TTATAA 2 cut(s) 336, 692
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 453, 618
PspPI GGNCC 2 cut(s) 354, 617
PspPPI RGGWCCY 1 cut(s) 617
PstNI CAGNNNCTG 2 cut(s) 800, 868
PsuI RGATCY 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 453, 928
RsaNI GTAC 2 cut(s) 452, 927
SalI GTCGAC 1 cut(s) 815
SaqAI TTAA 2 cut(s) 308, 483
SatI GCNGC 6 cut(s) 265, 510, 801, 852, 903, 906
Sau3AI GATC 3 cut(s) 433, 558, 827
Sau96I GGNCC 2 cut(s) 354, 617
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 2 cut(s) 157, 608
SduI GDGCHC 2 cut(s) 212, 630
SfaNI GCATC 2 cut(s) 257, 764
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
Sse9I AATT 5 cut(s) 33, 150, 276, 459, 855
SsiI CCGC 2 cut(s) 264, 939
StyD4I CCNGG 2 cut(s) 155, 606
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 321, 346, 926
TaiI ACGT 2 cut(s) 633, 731
TaqI TCGA 3 cut(s) 163, 408, 816
TasI AATT 5 cut(s) 33, 150, 276, 459, 855
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 4 cut(s) 44, 184, 488, 723
Tru1I TTAA 2 cut(s) 308, 483
Tru9I TTAA 2 cut(s) 308, 483
TscAI CASTG 4 cut(s) 217, 498, 849, 873
TseFI GTSAC 2 cut(s) 578, 782
TseI GCWGC 5 cut(s) 509, 800, 851, 902, 905
Tsp45I GTSAC 2 cut(s) 578, 782
TspDTI ATGAA 4 cut(s) 17, 176, 204, 690
TspGWI ACGGA 1 cut(s) 241
TspRI CASTG 4 cut(s) 217, 498, 849, 873
VpaK11BI GGWCC 1 cut(s) 617
VspI ATTAAT 1 cut(s) 483
XmiI GTMKAC 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.