pycom16g07940

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002227
Physical Location & Seq
Forward (+)
5257978 .. 5260558
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGGCAGGGAGAAACCATATAAGCGCCAGGGGCATCGATGGGGAAAATCCTAGGTTGTCATCTTTGTGTTCAGTGGAATCTGGCAGTAGTTGCTTCGCATGTAAATTTCCTGGTCCAAACTTTTTGAAGACAACTAGTGACTATCAGAATATCACAGTTCATTCTGTTACTCAATGCTCTCCTTCACCTGTAATCTCTTGCACAGAAAAGCCTCAAAGTTCTAATATATCACAAGTTATGGACGCAAATACAGAACATGGTCAATCACAGTCTTCTAATAAATCAGCATCATCTGGGGTCCTGCGAGATTCAGCTCGGTTAATGGAAGATTTCCTTGATCTTGCTAAAGAAAACACGGAGAAGGATCTAGAAACCTGCGGGATTCTTGGCGCTTTTCTTAAAAATGGAACATTCTATGTGACAACTCTGATCATACCCAAACAGGAATCAACTTCCAGCTCTTGTCAGGCAACTAACGATGAGGAAGCTTTTGCCATACAAAACGAACACTCACTTTTTCCGGTTGGATGGATCCATACACATCCTTCTCAAAGTTGTTTCATGTCATCAGTGGATTTGCACACTCATTATTCGTATCAGGTTATGATACCTGAGGCTTTTGCCATTGTCATGGCTCCAACTGATACCTCAAGGAGCTATGGAATATTTCGGCTATCTGATCCTGGCGGGATGAGCGTCCTGAAAGCGTGCCAGGAGCAAGGATTTCATCCTCACGGAGAAACAACTGACGGGAGTCCCGTTTTCGAGCACTGCTCTAATGTATACAAGAATTCAAATCTGAGGTTCGAAATCTTCGACTTGCGTTGA

Protein Analysis

276

Amino Acids

30.1

Weight (kDa)

5.4

Isoelectric Point (pI)

61.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 106 - 205 4.4e-16 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AccI GTMKAC 1 cut(s) 783
AciI CCGC 2 cut(s) 378, 687
AclWI GGATC 4 cut(s) 372, 526, 539, 674
AcsI RAATTY 2 cut(s) 104, 790
AgsI TTSAA 2 cut(s) 127, 795
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 4 cut(s) 26, 109, 682, 711
AluBI AGCT 4 cut(s) 314, 459, 488, 657
AluI AGCT 4 cut(s) 314, 459, 488, 657
Alw21I GWGCWC 1 cut(s) 771
AlwI GGATC 4 cut(s) 372, 526, 539, 674
ApoI RAATTY 2 cut(s) 104, 790
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 2 cut(s) 26, 392
AspS9I GGNCC 2 cut(s) 113, 298
AsuHPI GGTGA 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 807
AvaII GGWCC 2 cut(s) 113, 298
AvrII CCTAGG 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 612
BamHI GGATCC 1 cut(s) 531
BbsI GAAGAC 2 cut(s) 134, 264
Bbv12I GWGCWC 1 cut(s) 771
BccI CCATC 2 cut(s) 32, 522
BciT130I CCWGG 4 cut(s) 28, 111, 684, 713
BclI TGATCA 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 3 cut(s) 51, 135, 368
BfoI RGCGCY 2 cut(s) 27, 393
BfuAI ACCTGC 1 cut(s) 383
BlnI CCTAGG 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 28, 111, 684, 713
Bme18I GGWCC 2 cut(s) 113, 298
BmgT120I GGNCC 2 cut(s) 113, 298
BmiI GGNNCC 3 cut(s) 299, 533, 636
BmrFI CCNGG 4 cut(s) 28, 111, 684, 713
BmsI GCATC 2 cut(s) 42, 296
BoxI GACNNNNGTC 1 cut(s) 753
BpiI GAAGAC 2 cut(s) 134, 264
BplI GAGNNNNNCTC 2 cut(s) 758, 790
Bpu14I TTCGAA 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 634
Bsa29I ATCGAT 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 27, 50
BsaWI WCCGGW 1 cut(s) 520
Bse21I CCTNAGG 1 cut(s) 612
BseBI CCWGG 4 cut(s) 28, 111, 684, 713
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 27, 50
BseGI GGATG 4 cut(s) 533, 541, 696, 727
BseMII CTCAG 2 cut(s) 603, 791
BshVI ATCGAT 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 1 cut(s) 521
BslFI GGGAC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 741
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 1 cut(s) 771
Bsp143I GATC 5 cut(s) 337, 364, 429, 531, 679
BspACI CCGC 2 cut(s) 378, 687
BspCNI CTCAG 2 cut(s) 604, 792
BspDI ATCGAT 1 cut(s) 36
BspLI GGNNCC 3 cut(s) 299, 533, 636
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 4 cut(s) 372, 526, 539, 674
BspT104I TTCGAA 1 cut(s) 807
BssECI CCNNGG 2 cut(s) 27, 50
BssMI GATC 5 cut(s) 337, 364, 429, 531, 679
BssNAI GTATAC 1 cut(s) 784
BssT1I CCWWGG 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 784
Bst2UI CCWGG 4 cut(s) 28, 111, 684, 713
Bst4CI ACNGT 2 cut(s) 157, 270
BstAPI GCANNNNNTGC 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 807
BstC8I GCNNGC 1 cut(s) 709
BstDEI CTNAG 2 cut(s) 612, 800
BstF5I GGATG 4 cut(s) 533, 541, 696, 727
BstH2I RGCGCY 2 cut(s) 27, 393
BstHHI GCGC 2 cut(s) 26, 392
BstKTI GATC 5 cut(s) 340, 367, 432, 534, 682
BstMBI GATC 5 cut(s) 337, 364, 429, 531, 679
BstMWI GCNNNNNNNGC 3 cut(s) 30, 90, 693
BstNI CCWGG 4 cut(s) 28, 111, 684, 713
BstNSI RCATGY 1 cut(s) 102
BstPAI GACNNNNGTC 1 cut(s) 753
BstSCI CCNGG 4 cut(s) 26, 109, 682, 711
BstV2I GAAGAC 2 cut(s) 134, 264
BstX2I RGATCY 2 cut(s) 364, 531
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 2 cut(s) 364, 531
BstZ17I GTATAC 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 612
BsuTUI ATCGAT 1 cut(s) 36
BtsCI GGATG 4 cut(s) 533, 541, 696, 727
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 3 cut(s) 78, 576, 769
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 709
CfoI GCGC 2 cut(s) 26, 392
Cfr13I GGNCC 2 cut(s) 113, 298
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 2 cut(s) 251, 685
CviAII CATG 4 cut(s) 99, 257, 562, 631
CviJI RGCY 8 cut(s) 211, 314, 459, 488, 617, 635, 657, 673
CviKI_1 RGCY 8 cut(s) 211, 314, 459, 488, 617, 635, 657, 673
DdeI CTNAG 2 cut(s) 612, 800
DpnI GATC 5 cut(s) 339, 366, 431, 533, 681
DpnII GATC 5 cut(s) 337, 364, 429, 531, 679
Eco130I CCWWGG 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 113, 298
Eco81I CCTNAGG 1 cut(s) 612
EcoO109I RGGNCCY 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 4 cut(s) 26, 109, 682, 711
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 102, 260, 565, 634
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 741
FatI CATG 4 cut(s) 98, 256, 561, 630
FauI CCCGC 2 cut(s) 371, 680
FbaI TGATCA 1 cut(s) 429
FblI GTMKAC 1 cut(s) 783
FokI GGATG 4 cut(s) 528, 540, 703, 714
FspBI CTAG 3 cut(s) 51, 135, 368
GlaI GCGC 2 cut(s) 25, 391
HaeII RGCGCY 2 cut(s) 27, 393
HapII CCGG 1 cut(s) 521
HgaI GACGC 2 cut(s) 251, 685
HhaI GCGC 2 cut(s) 26, 392
Hin1II CATG 4 cut(s) 102, 260, 565, 634
Hin6I GCGC 2 cut(s) 24, 390
HinP1I GCGC 2 cut(s) 24, 390
HindIII AAGCTT 1 cut(s) 486
HinfI GANTC 5 cut(s) 77, 308, 382, 446, 754
HpaII CCGG 1 cut(s) 521
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 4 cut(s) 147, 429, 679, 801
Hpy188III TCNNGA 2 cut(s) 368, 700
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 3 cut(s) 192, 355, 555
HpyCH4III ACNGT 2 cut(s) 157, 270
HpyCH4V TGCA 2 cut(s) 201, 580
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 90, 693
HpyF3I CTNAG 2 cut(s) 612, 800
Hsp92II CATG 4 cut(s) 102, 260, 565, 634
HspAI GCGC 2 cut(s) 24, 390
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 5 cut(s) 337, 364, 429, 531, 679
LmnI GCTCC 3 cut(s) 640, 654, 715
LweI GCATC 2 cut(s) 42, 296
MaeI CTAG 3 cut(s) 51, 135, 368
MaeIII GTNAC 3 cut(s) 137, 166, 418
MalI GATC 5 cut(s) 339, 366, 431, 533, 681
MboI GATC 5 cut(s) 337, 364, 429, 531, 679
MboII GAAGA 4 cut(s) 139, 264, 338, 805
MflI RGATCY 2 cut(s) 364, 531
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 2 cut(s) 104, 790
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 2 cut(s) 505, 662
MnlI CCTC 6 cut(s) 222, 475, 607, 658, 741, 795
MseI TTAA 2 cut(s) 320, 399
MslI CAYNNNNRTG 2 cut(s) 64, 629
MspI CCGG 1 cut(s) 521
MspR9I CCNGG 4 cut(s) 28, 111, 684, 713
MvaI CCWGG 4 cut(s) 28, 111, 684, 713
MwoI GCNNNNNNNGC 3 cut(s) 30, 90, 693
NdeII GATC 5 cut(s) 337, 364, 429, 531, 679
NlaIII CATG 4 cut(s) 102, 260, 565, 634
NlaIV GGNNCC 3 cut(s) 299, 533, 636
NmuCI GTSAC 2 cut(s) 137, 418
NspI RCATGY 1 cut(s) 102
NspV TTCGAA 1 cut(s) 807
PcsI WCGNNNNNNNCGW 2 cut(s) 756, 813
PfeI GAWTC 4 cut(s) 77, 308, 382, 446
PleI GAGTC 1 cut(s) 762
PpsI GAGTC 1 cut(s) 762
PpuMI RGGWCCY 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 4 cut(s) 26, 109, 682, 711
PspGI CCWGG 4 cut(s) 26, 109, 682, 711
PspN4I GGNNCC 3 cut(s) 299, 533, 636
PspPI GGNCC 2 cut(s) 113, 298
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 364, 531
RseI CAYNNNNRTG 2 cut(s) 64, 629
SaqAI TTAA 2 cut(s) 320, 399
Sau3AI GATC 5 cut(s) 337, 364, 429, 531, 679
Sau96I GGNCC 2 cut(s) 113, 298
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 4 cut(s) 28, 111, 684, 713
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 2 cut(s) 42, 296
SfuI TTCGAA 1 cut(s) 807
SinI GGWCC 2 cut(s) 113, 298
SmiMI CAYNNNNRTG 2 cut(s) 64, 629
SmlI CTYRAG 1 cut(s) 649
SmoI CTYRAG 1 cut(s) 649
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 2 cut(s) 104, 790
SsiI CCGC 2 cut(s) 378, 687
SspI AATATT 1 cut(s) 666
SspMI CTAG 3 cut(s) 51, 135, 368
StyD4I CCNGG 4 cut(s) 26, 109, 682, 711
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 157, 270
TaqI TCGA 4 cut(s) 36, 765, 807, 816
TasI AATT 2 cut(s) 104, 790
TfiI GAWTC 4 cut(s) 77, 308, 382, 446
Tru1I TTAA 2 cut(s) 320, 399
Tru9I TTAA 2 cut(s) 320, 399
TscAI CASTG 3 cut(s) 78, 576, 776
TseFI GTSAC 2 cut(s) 137, 418
Tsp45I GTSAC 2 cut(s) 137, 418
TspDTI ATGAA 3 cut(s) 149, 550, 716
TspGWI ACGGA 2 cut(s) 371, 750
TspRI CASTG 3 cut(s) 78, 576, 776
VpaK11BI GGWCC 2 cut(s) 113, 298
XapI RAATTY 2 cut(s) 104, 790
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 1 cut(s) 102
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 783
XspI CTAG 3 cut(s) 51, 135, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.