pycom16g09790

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003470
Physical Location & Seq
Forward (+)
6685855 .. 6690466
4612 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3150 bp
ATGGTTCTTGGGATAAGATCAAAGAGCAGGAAGAGCGCGGCCGTTGAGGTTGATTACCTCATCCATGTGCAGGAACTCAAGCCATGGCCCTCGTCTCAGGCTTTGAGATCTGTTCAGTCTGTTATGCTTCAGTGGGAAAACGGTGATCAGGTTTCTGGATCGTGTATTTGCAATGTTGGTGATGCGAAGATTGAATTTGGCGAGTCTTTCACGCTTCCAGTCACTTTATACAAGGAGAAATCAAGAAAAAACGCAGTTCGCGACACTTACCAGAAGAACAACTTGGAGTTTTACTTGTATGAACCTCGAAAGGACAAGGGAGTGAAAGGTCAACTCTTGGGGTCGGCTGAGATCAATCTTGCAGACTATGGGATCATTGTGGAAACCCGAAATGTGAGCACTCCGCTGAACTGGAAGAAGAGTTTTAAGAGTTCAGCGGAAACGGTTCTTTCTGTTAGTATTCAGCCGTGTGGTAAACCGAGCTCCAGCTTGACACCAAAGAGCAGCTTGTCCAGAGAAGAGTCTCTGGAAAATGAGGGAACTGAATCTGTTCCAGGGTCTGTGAATGATGGCACTGACGAGATTGCCTCTTTTACCGATGATGAGTTTGATGATGATGTTTCCTCGCATCCATCACACACTGTAAACTCCTCGGCTGTTGAGACAACGGTCAGTTCATCACCTAGTGGTGAAAAGAATGTATTAGAATCAACAGCCAATGGCACTAGGAGGACTTATGGGGAGCCTGCTGTACAGTCAATAGCAGCACCTGCAAGCACAGGGGTGACTTCAGTGGCTAAAGCATTAGAGCACCAAAATGGAAGTTCATCTCCACTGTCGTCAATAGGCTCTTCCTCCATTCTGCTGAATTCTGCAAATGATCGTGCTTCTTTGCCTAACAGTTCAAAGCAGAGTGTTTCAATGCCAATCCTGAAAAAATCAATCACCCACTCTGTTCATTCAGTGTCTTCACCCTTTGGCTATCAAGACAGCCATCAGGAATCTGGTTACCATAATTTTAAGGACAACAGAATTCACATAACTCGTTCTAACAGAAGTGCCAGAATGCATGAAAATGCTCAAGATCTTATCAGGGACAATATTGTCCGTAACCATGCTGCTGAAGGTTCATCCTCAAGCACGTCTATTCAGGAGGACACAAACTCCACATTTGCAAGCAATGCAGATTCACAAGCCTTTCGAGAGGATGGTCATCTCTTAAAGGCAAATCATTATACTTTTGATGACAAATTAGCTTCTCGATTTTCACAAGATGCTACCAGAAAACAAGTCAGGTTAAAGAGTGAAACTTTTACACCCGGCAGGAGAGTTGTTGGAGTGCAGGGAGGTAACGTTAAAAGTAATGAACTAAAACATGTAAAGTCTGTGCAGTTACCTTTAGTATCAGCTCAGAACAATAGACAGTCAAGCAATAATGAGCTTCTCGAAAAATCAAAGGAAGCTGAAATTCCTAAAGATGCTTATGTTCATGGTAGAATTAGCGCGACAAGTGAAAGAGAAGAAAAAACAGTCAGCTTTTCCGATGGAAAAGTTGACTTGGAGTCTACAATTGAATTGCTTAAGGAAGAACTGAGAGAAGCTGCTGCTGTTGAGATTGGCCTTTACTCTGTTGCCGCTGAGCATGGGAGCCACACAAATAGGATTCATGCTCCTGCTCGTCGACTTTCTAGATTCTATTTTCATGCTTGCAAAACAAGCTCTGGAGCTAAGAAGGCAAATGCAGCAAGAGCTGCTATCACTGGACTAATCTTGGTTTCTAAAGCATGCGGAAATGATGTTGCTAGGTTGACATTCTGGTTGTCGAATTCAATTGTGTTGAGAGCTATTATCAGCCAGTCCTTGCCCTGCAGTAAAGTTGGCGCTGAGGGGGTTTTAAAAGCAAAGAATGGGTTTCTTCCTCACAAGGAAGAGAACAATTGCACTCTTGAAAGTTTTGATGATTGGGAGGATCCGCAGATATTTATGGCTGCTTTGGAAAAGTTTGAAGGTTGGATCTTCTCCCGAATTGTTGAGTCTGTATGGTGGCAGAATATGACTCCATATATGCAGTCAGCGGCTGCGAAGGGCTCAAGCTCAAGGAAAGCCAATGGAAGGAAGCATGGTTTGGGTGGTCATGAGCAAGGTAACTTTTCTATGGAGCTGTGGAAGAAGGCCTTCAAAGATGCCTGTGAAAGGCTCTGTCCTGCAAGAGCTGGTGGACATGAGTGTGGCTGCTTGCCTCTGCTGGCTCGTTTGGTAATGGAGCAATTAGTGGATAGATTGGATGTGGCTATGTTCAATGCTATTCTTCGTGAAAATGCTGAAGAGATGCCCACAGATCCTGTGTCCGACCCCATAAGTGATTCTAAGGTTCTCCCTATTCCAGCTGGAAAGTCGAGCTTTGGGGCTGGTGCACAGCTAAAAAATGCGATTGGAAGCTGGTCTAGATGGCTTACTGATCTATTTGGCATTGATGATACTGATGCCCCTGATGATAATACTAAACTCAGTGATCACAAGGGACAAGAGTGCCCAACATCCTTTAAAGCTTTCCGTCTTCTTAATGCTTTGAGTGACCTCATGATGCTTCCATTTGATATGCTGGCAGATAAATCCACCCGGCAAGAGGTATGCCCTACATTTGGTGCATCATTGATCAAGAGGGTTATATACAATTTTGTTCCAGATGAGTTTTGCCCAGACCCAATTCCCACGGCAGTTTTTGAGGCTCTTGATTATGAGGTACCTAAGAGGCCCTCGAATATCTCGACACGTAACGATTCAGCTTATATACTGAACTTCATTCCTGTTCGAATGGAGAATTCAGAAGATAAAACTGACTCTGCTGCAAGTTTCCCATGCAACGCAAATCCTACAATCTATTCACCACCTCCAGCTGCTTCACTATTAAGCATTATAGGAGAGGTAGGAAGTCTAACTCTCTCGAGGAGTGGGTCATCAGTGCTTAAGAAATCGTATACAAGTGATGATGAGCTCGATGAGTTGGATTCACCGATGACATCAATCATCATAGACAATTCCCCGTTTTCTCCCAGTCCATTGGCAGCCAACTCAACACCGAAGTGGAAGGGTGGTCGGAATGCTGTCAGGTATCAACTCCTCCGTGAAGTTTGGAAGGATAGCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1050

Amino Acids

114.74

Weight (kDa)

6.06

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 12 - 157 3.1e-14 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 778
AasI GACNNNNNNGTC 1 cut(s) 1103
Acc36I ACCTGC 1 cut(s) 778
Acc65I GGTACC 1 cut(s) 2743
AccB1I GGYRCC 1 cut(s) 2743
AccI GTMKAC 3 cut(s) 1565, 1681, 2978
AccII CGCG 3 cut(s) 38, 261, 1505
AciI CCGC 7 cut(s) 38, 404, 437, 1635, 1788, 1973, 2075
AclI AACGTT 1 cut(s) 1353
AclWI GGATC 6 cut(s) 166, 380, 1964, 1977, 2021, 2333
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 6 cut(s) 194, 868, 1032, 1467, 1825, 2821
AcuI CTGAAG 4 cut(s) 113, 774, 1143, 2343
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 753, 2745
AfiI CCNNNNNNNGG 5 cut(s) 70, 2112, 2193, 2626, 2642
AflII CTTAAG 2 cut(s) 1580, 2966
AflIII ACRYGT 2 cut(s) 1375, 2771
AgsI TTSAA 9 cut(s) 194, 906, 921, 1574, 1830, 1949, 2006, 2179, 2299
AhdI GACNNNNNGTC 1 cut(s) 1561
AjiI CACGTC 1 cut(s) 1143
AjnI CCWGG 1 cut(s) 553
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 2108, 2140
AleI CACNNNNGTG 2 cut(s) 782, 3082
Alw21I GWGCWC 5 cut(s) 401, 485, 813, 2416, 2997
Alw26I GTCTC 3 cut(s) 99, 528, 656
Alw44I GTGCAC 1 cut(s) 2412
AlwI GGATC 6 cut(s) 166, 380, 1964, 1977, 2021, 2333
AlwNI CAGNNNCTG 4 cut(s) 560, 770, 2078, 2342
Ama87I CYCGRG 1 cut(s) 2944
AoxI GGCC 5 cut(s) 39, 86, 1618, 2172, 2753
ApaLI GTGCAC 1 cut(s) 2412
ApoI RAATTY 6 cut(s) 194, 868, 1032, 1467, 1825, 2821
Asp700I GAANNNNTTC 3 cut(s) 444, 549, 2174
Asp718I GGTACC 1 cut(s) 2743
AspLEI GCGC 3 cut(s) 38, 1505, 1883
AspS9I GGNCC 2 cut(s) 87, 2754
AsuC2I CCSGG 2 cut(s) 1320, 2620
AsuHPI GGTGA 9 cut(s) 155, 191, 672, 701, 796, 937, 963, 2877, 3003
AsuII TTCGAA 1 cut(s) 2812
AvaI CYCGRG 1 cut(s) 2944
BaeGI GKGCMC 2 cut(s) 2416, 2534
BamHI GGATCC 1 cut(s) 1969
BanI GGYRCC 1 cut(s) 2743
BanII GRGCYC 3 cut(s) 485, 2090, 2997
BbsI GAAGAC 2 cut(s) 960, 2549
Bbv12I GWGCWC 5 cut(s) 401, 485, 813, 2416, 2997
BbvCI CCTCAGC 1 cut(s) 1884
BccI CCATC 6 cut(s) 563, 640, 1002, 1202, 1538, 2442
BceAI ACGGC 3 cut(s) 26, 451, 2730
BciT130I CCWGG 1 cut(s) 555
BclI TGATCA 3 cut(s) 145, 2512, 2655
BcnI CCSGG 2 cut(s) 1320, 2620
BcoDI GTCTC 3 cut(s) 99, 528, 656
BfaI CTAG 5 cut(s) 684, 726, 1689, 1803, 2445
BfmI CTRYAG 1 cut(s) 1867
BfoI RGCGCY 1 cut(s) 1884
BfrI CTTAAG 2 cut(s) 1580, 2966
BfuAI ACCTGC 1 cut(s) 778
BglII AGATCT 2 cut(s) 107, 1084
BlpI GCTNAGC 1 cut(s) 1638
Bme1390I CCNGG 3 cut(s) 555, 1320, 2620
BmeRI GACNNNNNGTC 1 cut(s) 1561
BmeT110I CYCGRG 1 cut(s) 2944
BmgBI CACGTC 1 cut(s) 1143
BmgT120I GGNCC 2 cut(s) 87, 2754
BmiI GGNNCC 4 cut(s) 744, 1649, 1971, 2745
BmrFI CCNGG 3 cut(s) 555, 1320, 2620
BmrI ACTGGG 1 cut(s) 3048
BmsI GCATC 9 cut(s) 172, 637, 1264, 1468, 2173, 2319, 2473, 2574, 2657
BmuI ACTGGG 1 cut(s) 3048
BoxI GACNNNNGTC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 960, 2549
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 3 cut(s) 469, 1743, 2877
Bpu10I CCTNAGC 1 cut(s) 1884
Bpu1102I GCTNAGC 1 cut(s) 1638
Bpu14I TTCGAA 1 cut(s) 2812
BpuEI CTTGAG 5 cut(s) 62, 1065, 1120, 2074, 2080
BpuMI CCSGG 2 cut(s) 1320, 2620
BsaAI YACGTR 1 cut(s) 2774
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 4 cut(s) 83, 554, 651, 2712
BsaXI ACNNNNNCTCC 4 cut(s) 1148, 1178, 1338, 1368
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 2112, 2193, 2626, 2642
Bse1I ACTGG 5 cut(s) 218, 416, 1765, 1855, 3054
Bse3DI GCAATG 2 cut(s) 178, 1186
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 4 cut(s) 83, 554, 651, 2712
BseGI GGATG 6 cut(s) 60, 628, 1130, 1213, 2290, 2537
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 5 cut(s) 70, 2112, 2193, 2626, 2642
BseMI GCAATG 2 cut(s) 178, 1186
BseMII CTCAG 7 cut(s) 110, 339, 1424, 1583, 1629, 1875, 2521
BseNI ACTGG 5 cut(s) 218, 416, 1765, 1855, 3054
BseRI GAGGAG 3 cut(s) 640, 2962, 3110
BseSI GKGCMC 2 cut(s) 2416, 2534
BseX3I CGGCCG 1 cut(s) 39
BsgI GTGCAG 3 cut(s) 89, 1361, 1409
Bsh1236I CGCG 3 cut(s) 38, 261, 1505
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 5 cut(s) 41, 88, 1620, 2174, 2755
BshNI GGYRCC 1 cut(s) 2743
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 5 cut(s) 401, 485, 813, 2416, 2997
BsiHKCI CYCGRG 1 cut(s) 2944
BsiSI CCGG 2 cut(s) 1320, 2620
BslFI GGGAC 2 cut(s) 1109, 2535
BslI CCNNNNNNNGG 5 cut(s) 70, 2112, 2193, 2626, 2642
BsmAI GTCTC 3 cut(s) 99, 528, 656
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 1109, 2535
BsmI GAATGC 2 cut(s) 1071, 3106
BsnI GGCC 5 cut(s) 41, 88, 1620, 2174, 2755
BsoBI CYCGRG 1 cut(s) 2944
Bsp119I TTCGAA 1 cut(s) 2812
Bsp1286I GDGCHC 7 cut(s) 401, 485, 813, 2090, 2416, 2534, 2997
Bsp1407I TGTACA 1 cut(s) 751
Bsp1720I GCTNAGC 1 cut(s) 1638
Bsp19I CCATGG 1 cut(s) 83
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 7 cut(s) 38, 404, 437, 1635, 1788, 1973, 2075
BspANI GGCC 5 cut(s) 41, 88, 1620, 2174, 2755
BspCNI CTCAG 7 cut(s) 109, 340, 1423, 1584, 1630, 1876, 2520
BspFNI CGCG 3 cut(s) 38, 261, 1505
BspHI TCATGA 2 cut(s) 2134, 2580
BspLI GGNNCC 4 cut(s) 744, 1649, 1971, 2745
BspMAI CTGCAG 1 cut(s) 1871
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 6 cut(s) 166, 380, 1964, 1977, 2021, 2333
BspQI GCTCTTC 2 cut(s) 26, 856
BspT104I TTCGAA 1 cut(s) 2812
BspT107I GGYRCC 1 cut(s) 2743
BspTI CTTAAG 2 cut(s) 1580, 2966
BsrDI GCAATG 2 cut(s) 178, 1186
BsrGI TGTACA 1 cut(s) 751
BsrI ACTGG 5 cut(s) 218, 416, 1765, 1855, 3054
BssECI CCNNGG 4 cut(s) 83, 554, 651, 2712
BssNAI GTATAC 1 cut(s) 2979
BssT1I CCWWGG 1 cut(s) 83
Bst1107I GTATAC 1 cut(s) 2979
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1531
Bst6I CTCTTC 6 cut(s) 26, 413, 513, 856, 1923, 2319
BstAFI CTTAAG 2 cut(s) 1580, 2966
BstAPI GCANNNNNTGC 3 cut(s) 770, 1181, 1751
BstAUI TGTACA 1 cut(s) 751
BstBAI YACGTR 1 cut(s) 2774
BstBI TTCGAA 1 cut(s) 2812
BstC8I GCNNGC 8 cut(s) 747, 775, 1177, 1708, 1786, 2237, 2247, 2604
BstDSI CCRYGG 2 cut(s) 83, 2712
BstEII GGTNACC 1 cut(s) 1007
BstENI CCTNNNNNAGG 1 cut(s) 2191
BstF5I GGATG 6 cut(s) 60, 628, 1130, 1213, 2290, 2537
BstFNI CGCG 3 cut(s) 38, 261, 1505
BstH2I RGCGCY 1 cut(s) 1884
BstHHI GCGC 3 cut(s) 38, 1505, 1883
BstMAI GTCTC 3 cut(s) 99, 528, 656
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 8 cut(s) 33, 770, 1181, 1716, 1733, 1742, 1748, 1751
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 2 cut(s) 1379, 1788
BstPAI GACNNNNGTC 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 3 cut(s) 553, 1318, 2618
BstSFI CTRYAG 1 cut(s) 1867
BstSLI GKGCMC 2 cut(s) 2416, 2534
BstUI CGCG 3 cut(s) 38, 261, 1505
BstV2I GAAGAC 2 cut(s) 960, 2549
BstX2I RGATCY 5 cut(s) 107, 1084, 1969, 2013, 2338
BstXI CCANNNNNNTGG 1 cut(s) 3061
BstYI RGATCY 5 cut(s) 107, 1084, 1969, 2013, 2338
BstZ17I GTATAC 1 cut(s) 2979
BstZI CGGCCG 1 cut(s) 39
BsuRI GGCC 5 cut(s) 41, 88, 1620, 2174, 2755
BtgI CCRYGG 2 cut(s) 83, 2712
BtrI CACGTC 1 cut(s) 1143
BtsCI GGATG 6 cut(s) 60, 628, 1130, 1213, 2290, 2537
BtsIMutI CAGTG 9 cut(s) 137, 573, 639, 798, 833, 969, 1758, 2515, 2967
BtuMI TCGCGA 1 cut(s) 261
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 8 cut(s) 747, 775, 1177, 1708, 1786, 2237, 2247, 2604
CaiI CAGNNNCTG 4 cut(s) 560, 770, 2078, 2342
CciI TCATGA 2 cut(s) 2134, 2580
CfoI GCGC 3 cut(s) 38, 1505, 1883
Cfr13I GGNCC 2 cut(s) 87, 2754
Csp6I GTAC 2 cut(s) 752, 2744
CviQI GTAC 2 cut(s) 752, 2744
DraI TTTAAA 2 cut(s) 1896, 2545
DraIII CACNNNGTG 1 cut(s) 686
DrdI GACNNNNNNGTC 1 cut(s) 1103
DriI GACNNNNNGTC 1 cut(s) 1561
DseDI GACNNNNNNGTC 1 cut(s) 1103
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 6 cut(s) 26, 413, 513, 856, 1923, 2319
Eam1105I GACNNNNNGTC 1 cut(s) 1561
EarI CTCTTC 6 cut(s) 26, 413, 513, 856, 1923, 2319
Ecl136II GAGCTC 2 cut(s) 483, 2995
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 83
Eco147I AGGCCT 1 cut(s) 2174
Eco24I GRGCYC 3 cut(s) 485, 2090, 2997
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 2 cut(s) 483, 2995
Eco57I CTGAAG 4 cut(s) 113, 774, 1143, 2343
Eco88I CYCGRG 1 cut(s) 2944
Eco91I GGTNACC 1 cut(s) 1007
EcoICRI GAGCTC 2 cut(s) 483, 2995
EcoNI CCTNNNNNAGG 1 cut(s) 2191
EcoO109I RGGNCCY 1 cut(s) 2754
EcoO65I GGTNACC 1 cut(s) 1007
EcoRI GAATTC 4 cut(s) 868, 1032, 1825, 2821
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 1071
EcoT38I GRGCYC 3 cut(s) 485, 2090, 2997
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 99
FalI AAGNNNNNCTT 8 cut(s) 266, 298, 491, 523, 2159, 2191, 2384, 2416
FaqI GGGAC 2 cut(s) 1109, 2535
FbaI TGATCA 3 cut(s) 145, 2512, 2655
FblI GTMKAC 3 cut(s) 1565, 1681, 2978
FokI GGATG 6 cut(s) 47, 615, 1117, 1220, 2297, 2524
FriOI GRGCYC 3 cut(s) 485, 2090, 2997
FspBI CTAG 5 cut(s) 684, 726, 1689, 1803, 2445
GlaI GCGC 3 cut(s) 37, 1504, 1882
GsuI CTGGAG 3 cut(s) 469, 1743, 2877
HaeII RGCGCY 1 cut(s) 1884
HaeIII GGCC 5 cut(s) 41, 88, 1620, 2174, 2755
HapII CCGG 2 cut(s) 1320, 2620
HhaI GCGC 3 cut(s) 38, 1505, 1883
Hin6I GCGC 3 cut(s) 36, 1503, 1881
HinP1I GCGC 3 cut(s) 36, 1503, 1881
HincII GTYRAC 4 cut(s) 332, 1555, 1682, 1809
HindII GTYRAC 4 cut(s) 332, 1555, 1682, 1809
HindIII AAGCTT 1 cut(s) 2547
HpaII CCGG 2 cut(s) 1320, 2620
HphI GGTGA 9 cut(s) 155, 191, 672, 701, 796, 937, 963, 2877, 3003
Hpy188I TCNGA 5 cut(s) 1413, 1543, 2350, 2827, 3099
Hpy99I CGWCG 1 cut(s) 1683
HpyAV CCTTC 9 cut(s) 1118, 1726, 2000, 2077, 2106, 2164, 2185, 3082, 3130
HpyCH4III ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1531
HpyCH4IV ACGT 3 cut(s) 1142, 1353, 2773
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 770, 1181, 1716, 1733, 1742, 1748, 1751
HpySE526I ACGT 3 cut(s) 1142, 1353, 2773
HspAI GCGC 3 cut(s) 36, 1503, 1881
KpnI GGTACC 1 cut(s) 2747
Ksp22I TGATCA 3 cut(s) 145, 2512, 2655
LguI GCTCTTC 2 cut(s) 26, 856
LmnI GCTCC 7 cut(s) 488, 742, 1647, 1675, 1724, 2158, 2263
LweI GCATC 9 cut(s) 172, 637, 1264, 1468, 2173, 2319, 2473, 2574, 2657
MaeI CTAG 5 cut(s) 684, 726, 1689, 1803, 2445
MaeII ACGT 3 cut(s) 1142, 1353, 2773
MaeIII GTNAC 9 cut(s) 220, 784, 1007, 1109, 1349, 1392, 2144, 2573, 2774
MfeI CAATTG 3 cut(s) 1569, 1830, 1936
MflI RGATCY 5 cut(s) 107, 1084, 1969, 2013, 2338
MhlI GDGCHC 7 cut(s) 401, 485, 813, 2090, 2416, 2534, 2997
MlyI GAGTC 6 cut(s) 212, 530, 1571, 2042, 2050, 2834
MmeI TCCRAC 5 cut(s) 1315, 1991, 2373, 2985, 3077
Mph1103I ATGCAT 1 cut(s) 1071
MroXI GAANNNNTTC 3 cut(s) 444, 549, 2174
MslI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2226, 3082
MspA1I CMGCKG 6 cut(s) 406, 437, 1637, 2075, 2387, 2897
MspCI CTTAAG 2 cut(s) 1580, 2966
MspI CCGG 2 cut(s) 1320, 2620
MspR9I CCNGG 3 cut(s) 555, 1320, 2620
MunI CAATTG 3 cut(s) 1569, 1830, 1936
Mva1269I GAATGC 2 cut(s) 1071, 3106
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 3 cut(s) 38, 261, 1505
MwoI GCNNNNNNNGC 8 cut(s) 33, 770, 1181, 1716, 1733, 1742, 1748, 1751
NciI CCSGG 2 cut(s) 1320, 2620
NcoI CCATGG 1 cut(s) 83
NlaIV GGNNCC 4 cut(s) 744, 1649, 1971, 2745
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 3 cut(s) 220, 784, 2573
NruI TCGCGA 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 1071
NspI RCATGY 2 cut(s) 1379, 1788
NspV TTCGAA 1 cut(s) 2812
OliI CACNNNNGTG 2 cut(s) 782, 3082
PaeI GCATGC 1 cut(s) 1788
PaeR7I CTCGAG 1 cut(s) 2944
PagI TCATGA 2 cut(s) 2134, 2580
PaqCI CACCTGC 1 cut(s) 778
PceI AGGCCT 1 cut(s) 2174
PciI ACATGT 1 cut(s) 1375
PciSI GCTCTTC 2 cut(s) 26, 856
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 2765
PctI GAATGC 2 cut(s) 1071, 3106
PdmI GAANNNNTTC 3 cut(s) 444, 549, 2174
PfeI GAWTC 9 cut(s) 545, 707, 1001, 1187, 1663, 1692, 2363, 2780, 3008
PleI GAGTC 6 cut(s) 211, 529, 1570, 2041, 2050, 2834
PpsI GAGTC 6 cut(s) 211, 529, 1570, 2041, 2050, 2834
Ppu21I YACGTR 1 cut(s) 2774
PscI ACATGT 1 cut(s) 1375
PshAI GACNNNNGTC 1 cut(s) 668
Psp124BI GAGCTC 2 cut(s) 485, 2997
Psp1406I AACGTT 1 cut(s) 1353
Psp6I CCWGG 1 cut(s) 553
PspEI GGTNACC 1 cut(s) 1007
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 4 cut(s) 744, 1649, 1971, 2745
PspPI GGNCC 2 cut(s) 87, 2754
PstI CTGCAG 1 cut(s) 1871
PstNI CAGNNNCTG 4 cut(s) 560, 770, 2078, 2342
PsuI RGATCY 5 cut(s) 107, 1084, 1969, 2013, 2338
PvuII CAGCTG 2 cut(s) 2387, 2897
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 2 cut(s) 753, 2745
RsaNI GTAC 2 cut(s) 752, 2744
RseI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2226, 3082
SacI GAGCTC 2 cut(s) 485, 2997
SalI GTCGAC 1 cut(s) 1680
SapI GCTCTTC 2 cut(s) 26, 856
Sau96I GGNCC 2 cut(s) 87, 2754
SchI GAGTC 6 cut(s) 212, 530, 1571, 2042, 2050, 2834
ScrFI CCNGG 3 cut(s) 555, 1320, 2620
SduI GDGCHC 7 cut(s) 401, 485, 813, 2090, 2416, 2534, 2997
SfaNI GCATC 9 cut(s) 172, 637, 1264, 1468, 2173, 2319, 2473, 2574, 2657
SfcI CTRYAG 1 cut(s) 1867
Sfr274I CTCGAG 1 cut(s) 2944
SfuI TTCGAA 1 cut(s) 2812
SlaI CTCGAG 1 cut(s) 2944
SmiMI CAYNNNNRTG 6 cut(s) 65, 782, 816, 1074, 2226, 3082
SmlI CTYRAG 8 cut(s) 77, 1080, 1135, 1580, 2089, 2095, 2944, 2966
SmoI CTYRAG 8 cut(s) 77, 1080, 1135, 1580, 2089, 2095, 2944, 2966
SphI GCATGC 1 cut(s) 1788
SseBI AGGCCT 1 cut(s) 2174
SsiI CCGC 7 cut(s) 38, 404, 437, 1635, 1788, 1973, 2075
SspI AATATT 1 cut(s) 1102
SspMI CTAG 5 cut(s) 684, 726, 1689, 1803, 2445
SstI GAGCTC 2 cut(s) 485, 2997
StuI AGGCCT 1 cut(s) 2174
StyD4I CCNGG 3 cut(s) 553, 1318, 2618
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 9 cut(s) 143, 445, 643, 670, 756, 837, 902, 1425, 1531
TaiI ACGT 3 cut(s) 1145, 1356, 2776
TatI WGTACW 1 cut(s) 751
TauI GCSGC 3 cut(s) 41, 1637, 2078
TfiI GAWTC 9 cut(s) 545, 707, 1001, 1187, 1663, 1692, 2363, 2780, 3008
TscAI CASTG 9 cut(s) 137, 580, 646, 798, 840, 969, 1765, 2515, 2967
TseFI GTSAC 3 cut(s) 220, 784, 2573
Tsp45I GTSAC 3 cut(s) 220, 784, 2573
TspGWI ACGGA 3 cut(s) 1097, 2543, 3113
TspRI CASTG 9 cut(s) 137, 580, 646, 798, 840, 969, 1765, 2515, 2967
Vha464I CTTAAG 2 cut(s) 1580, 2966
VneI GTGCAC 1 cut(s) 2412
XagI CCTNNNNNAGG 1 cut(s) 2191
XapI RAATTY 6 cut(s) 194, 868, 1032, 1467, 1825, 2821
XbaI TCTAGA 2 cut(s) 1688, 2444
XceI RCATGY 2 cut(s) 1379, 1788
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XhoI CTCGAG 1 cut(s) 2944
XmiI GTMKAC 3 cut(s) 1565, 1681, 2978
XmnI GAANNNNTTC 3 cut(s) 444, 549, 2174
XspI CTAG 5 cut(s) 684, 726, 1689, 1803, 2445
Zsp2I ATGCAT 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.