pycom16g10770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010108
Physical Location & Seq
Forward (+)
7427920 .. 7429296
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGACAGCTCCAAACATGGCGAAGATCGCCGCTTCCCTGGAGAGGTCTCTCCAAAATTGCTCCCTAAACAACCACAACCACCACCACCACAACCACAGCAGCAGCGGCGGCGGCAGCATCACAGTCGACGGCGAAGGACAAGGCTCGAGCTTCACTGACGGGGTATTGGCCGCCACGGGGATGCAGGGCAGGTCATCGTCTTCAACCTCATCAACCTCAGACGCCACTTTAGAGCTCAACTCCCATGTCTCCCTTCCATACCATTGGGAACAATGCCTCGATTTAAAGACAGGGGAGGTATACTACCTAAACTGGAGGAACGGGATGAAGGCGAAAGAAGATCCGAGGACAAGAGGAGAGTACAGTGGAGATTTCTACTACTCGGAAGAAGATGAAAACAGCTCGTATGACAGCGAAGAGTCTTCGATCGAGTCGTCGCCATCTTCGTGCAGATACGAGCAGCACCAGCAGCCGATAGAGAATCTCAACAGCAACAACAACAATAATAATAATGTGTTGGTAGTGGCTGGCTGCAAAGTCTGTCTCATGTATTTCATGGTCCCGAAACAGCTCGAGGACTGCCCCAAATGCTCCGGTCAACTTCTCCACTTCGATCGATCCGAAAATGGCTCCCCATCGGTGATCGATCATCACCCGCTCACATTCATTTTCTTCTTAGTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.22

Weight (kDa)

5.16

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 180
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 2 cut(s) 126, 300
AciI CCGC 6 cut(s) 30, 105, 108, 111, 171, 656
AclWI GGATC 2 cut(s) 335, 612
AcoI YGGCCR 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 42, 177
AgsI TTSAA 1 cut(s) 204
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 5 cut(s) 8, 150, 235, 402, 571
AluI AGCT 5 cut(s) 8, 150, 235, 402, 571
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 51, 253, 548
AlwI GGATC 2 cut(s) 335, 612
Ama87I CYCGRG 2 cut(s) 145, 572
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 6 cut(s) 99, 102, 114, 460, 469, 531
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 644, 652
AvaI CYCGRG 2 cut(s) 145, 572
AvaII GGWCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 192, 414
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 6 cut(s) 111, 114, 126, 472, 481, 518
BccI CCATC 2 cut(s) 448, 643
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 38
BcoDI GTCTC 3 cut(s) 51, 253, 548
BfuAI ACCTGC 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 559
BmeT110I CYCGRG 2 cut(s) 145, 572
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 561, 631
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 2 cut(s) 126, 171
BpiI GAAGAC 2 cut(s) 192, 414
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 2 cut(s) 59, 334
Bsa29I ATCGAT 2 cut(s) 616, 645
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 36, 174, 344
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 177
Bse1I ACTGG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 38
BseCI ATCGAT 2 cut(s) 616, 645
BseDI CCNNGG 3 cut(s) 36, 174, 344
BseGI GGATG 2 cut(s) 186, 330
BseLI CCNNNNNNNGG 2 cut(s) 42, 177
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 6 cut(s) 111, 114, 126, 472, 481, 518
BsgI GTGCAG 1 cut(s) 469
Bsh1285I CGRYCG 2 cut(s) 429, 616
BshFI GGCC 1 cut(s) 170
BshVI ATCGAT 2 cut(s) 616, 645
BsiEI CGRYCG 2 cut(s) 429, 616
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 145, 572
BsiSI CCGG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 42, 177
BsmAI GTCTC 3 cut(s) 51, 253, 548
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 1 cut(s) 170
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 2 cut(s) 145, 572
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
BspACI CCGC 6 cut(s) 30, 105, 108, 111, 171, 656
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 230
BspDI ATCGAT 2 cut(s) 616, 645
BspLI GGNNCC 2 cut(s) 561, 631
BspMI ACCTGC 1 cut(s) 180
BspPI GGATC 2 cut(s) 335, 612
BspTNI GGTCTC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 658
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 36, 174, 344
BssMI GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
BssNAI GTATAC 1 cut(s) 301
BssNI GRCGYC 1 cut(s) 222
Bst1107I GTATAC 1 cut(s) 301
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 124, 365
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 3 cut(s) 217, 676, 684
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 186, 330
BstKTI GATC 7 cut(s) 27, 343, 429, 616, 620, 645, 649
BstMAI GTCTC 3 cut(s) 51, 253, 548
BstMBI GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
BstMCI CGRYCG 2 cut(s) 429, 616
BstMWI GCNNNNNNNGC 8 cut(s) 26, 105, 108, 111, 114, 466, 469, 588
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 6 cut(s) 111, 114, 126, 472, 481, 518
BstV2I GAAGAC 2 cut(s) 192, 414
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 264
BstYI RGATCY 1 cut(s) 340
BstZ17I GTATAC 1 cut(s) 301
Bsu15I ATCGAT 2 cut(s) 616, 645
BsuRI GGCC 1 cut(s) 170
BsuTUI ATCGAT 2 cut(s) 616, 645
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 186, 330
BtsIMutI CAGTG 2 cut(s) 153, 370
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 529
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 2 cut(s) 616, 645
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 361
CviAII CATG 4 cut(s) 16, 245, 547, 556
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 3 cut(s) 217, 676, 684
DpnI GATC 7 cut(s) 26, 342, 428, 615, 619, 644, 648
DpnII GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
DraI TTTAAA 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 235
Eco88I CYCGRG 2 cut(s) 145, 572
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 4 cut(s) 19, 248, 550, 559
FaiI YATR 7 cut(s) 17, 246, 259, 301, 408, 548, 557
FaqI GGGAC 1 cut(s) 545
FatI CATG 4 cut(s) 15, 244, 546, 555
FauI CCCGC 1 cut(s) 663
FblI GTMKAC 2 cut(s) 126, 300
FokI GGATG 2 cut(s) 193, 337
FriOI GRGCYC 1 cut(s) 237
GsuI CTGGAG 2 cut(s) 59, 334
HaeIII GGCC 1 cut(s) 170
HapII CCGG 1 cut(s) 594
HgaI GACGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 19, 248, 550, 559
HincII GTYRAC 2 cut(s) 127, 599
HindII GTYRAC 2 cut(s) 127, 599
HinfI GANTC 3 cut(s) 419, 431, 481
HpaII CCGG 1 cut(s) 594
HphI GGTGA 2 cut(s) 644, 652
Hpy166II GTNNAC 3 cut(s) 127, 301, 599
Hpy188I TCNGA 4 cut(s) 220, 345, 385, 622
Hpy188III TCNNGA 1 cut(s) 562
Hpy8I GTNNAC 3 cut(s) 127, 301, 599
Hpy99I CGWCG 2 cut(s) 131, 439
HpyAV CCTTC 3 cut(s) 128, 263, 322
HpyCH4III ACNGT 2 cut(s) 124, 365
HpyCH4V TGCA 3 cut(s) 184, 450, 534
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 105, 108, 111, 114, 466, 469, 588
HpyF3I CTNAG 3 cut(s) 217, 676, 684
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 19, 248, 550, 559
Kzo9I GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
LmnI GCTCC 4 cut(s) 13, 65, 596, 635
LpnPI CCDG 9 cut(s) 23, 50, 170, 175, 276, 298, 479, 513, 607
Lsp1109I GCAGC 6 cut(s) 111, 114, 126, 472, 481, 518
LweI GCATC 2 cut(s) 126, 171
MalI GATC 7 cut(s) 26, 342, 428, 615, 619, 644, 648
MbiI CCGCTC 1 cut(s) 658
MboI GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
MboII GAAGA 9 cut(s) 34, 192, 350, 398, 401, 414, 428, 435, 664
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 1 cut(s) 55
MlyI GAGTC 2 cut(s) 428, 440
MnlI CCTC 9 cut(s) 36, 217, 226, 287, 289, 309, 339, 347, 568
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 2 cut(s) 179, 445
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 1 cut(s) 594
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 8 cut(s) 26, 105, 108, 111, 114, 466, 469, 588
NdeII GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
NlaIII CATG 4 cut(s) 19, 248, 550, 559
NlaIV GGNNCC 2 cut(s) 561, 631
PaeR7I CTCGAG 2 cut(s) 145, 572
PcsI WCGNNNNNNNCGW 3 cut(s) 431, 443, 618
PfeI GAWTC 1 cut(s) 481
Ple19I CGATCG 2 cut(s) 429, 616
PleI GAGTC 2 cut(s) 427, 439
PpsI GAGTC 2 cut(s) 427, 439
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 561, 631
PspPI GGNCC 1 cut(s) 559
PspXI VCTCGAGB 2 cut(s) 145, 572
PsuI RGATCY 1 cut(s) 340
PvuI CGATCG 2 cut(s) 429, 616
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 2 cut(s) 179, 445
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 284
Sau3AI GATC 7 cut(s) 24, 340, 426, 613, 617, 642, 646
Sau96I GGNCC 1 cut(s) 559
SchI GAGTC 2 cut(s) 428, 440
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 2 cut(s) 126, 171
Sfr274I CTCGAG 2 cut(s) 145, 572
SinI GGWCC 1 cut(s) 559
SlaI CTCGAG 2 cut(s) 145, 572
SmiMI CAYNNNNRTG 2 cut(s) 179, 445
SmlI CTYRAG 2 cut(s) 145, 572
SmoI CTYRAG 2 cut(s) 145, 572
Sse9I AATT 1 cut(s) 55
SsiI CCGC 6 cut(s) 30, 105, 108, 111, 171, 656
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 124, 365
TaqI TCGA 9 cut(s) 126, 146, 279, 425, 429, 573, 612, 616, 645
TasI AATT 1 cut(s) 55
TatI WGTACW 1 cut(s) 360
TauI GCSGC 5 cut(s) 32, 108, 111, 114, 173
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 2 cut(s) 160, 370
TseI GCWGC 6 cut(s) 99, 102, 114, 460, 469, 531
TspDTI ATGAA 4 cut(s) 341, 408, 544, 655
TspRI CASTG 2 cut(s) 160, 370
VpaK11BI GGWCC 1 cut(s) 559
XhoI CTCGAG 2 cut(s) 145, 572
XmiI GTMKAC 2 cut(s) 126, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.