pycom16g11660

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005575
Physical Location & Seq
Forward (+)
8094201 .. 8097689
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
ATGAGATTTCTGGGGTCAGCCAACATGAAGCTTTTGTGGGTTGTGGGGTTTATGGGGTTACTGTTTTGCAGAAGAACAGATGGAACTTTTATTAGGGAAGTGAATGTCACCCAGGAACAAGACCTGCATTATACAGAGAGTAATGTGTATGGCTATGCAGCTTATGGTTATGGGGTTTTCACCAGACCCTTGATGGTGGGTCTCACTCTCATCAATGGAGCTGCTGCCAAAGGAGCAGTCTGCTTAGATGGAACATTACCTGCTTACCACATCCACCGCGGATACGGGTCAGGAGCAAACAGTTGGCTCATCCAGTTGGAGGGAGGAGGATGGTGTGATACAATCAGAAATTGTGTCTATCGTAAGAAAACTCGTCGTGGTTCATCAGCATATATGGAGAAGCAGATACCATTTTCCGGGATATTGAGCAACAAGGCTGGAGAAAACCCTGACTTTTACAACTGGAACAGAGTTAAGGTTCGATATTGTGATGGTGCATCTTTCAGCGGTGACAGTCAAAATGAGGCTGCCCAATTGCATTTTCGAGGGCAACGTATCTGGAAAGCTGCTATGGAAGACTTGATGTCAAAGGGAATGGGCTATGCTAATCAGGCTCTTCTTTCTGGATGTTCTGCTGGTGGTGTTGCAACTGTACTCCACTGTGATGGATTTCGTGGAATGTTTCCCGCAACTACTAAAGTCAAGTGCCTCAGCGATGGTGGATTGTTTCTTGACGCAATTGATGTATCTGGTAGGCGCACTTTGAGAAGTATGTTTAGAAGAGTGGTTAGCCTACAGGGGGTGCAAAAGAATCTCCCACGGAGTTGTACCATTCGCCTCAACCCGACTTTGTGCTTTTTCCCCCAGCACTTAATTGCAAGTGTCAAGACTCCCCTTTTTCTGGTCAATGCAGCTTATGATACATGGCAGATCCAGGCCAGTTTAGCTCCACGAACAGCAGACCCTAATGGCCATTGGCATGCATGCACAAACAATAATGCAAATTGTGCTGCATGGCAAATTAAGTTTTTGCAAGGTTTCCGGAATCAGATGCTCAAAGCTGTAAGCGGATTCTCGAGGGCCGGTAAAAATGGTTTGTTCATAAATTCTTGCTTTTCTCATTGCCAGACCGAGAGACAAGACACCTGGTTTTCTCAAAATCCTCCCCTTATTGGAAATAAGGGGATAGCAAAATCAGTTGGAAACTGGTATTTCGATCGGTATAGTATCAAAGCTATTGATTGTCCGTACCCTTGTGACAAGACCTGCCATAATCTGGTGTTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

430

Amino Acids

47.71

Weight (kDa)

9.42

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 63 - 424 6.9e-170 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 132, 268, 1274
AccB7I CCANNNNNTGG 1 cut(s) 1276
AccII CGCG 1 cut(s) 279
AccIII TCCGGA 1 cut(s) 1041
AciI CCGC 5 cut(s) 277, 279, 507, 687, 1068
AclWI GGATC 1 cut(s) 925
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 1 cut(s) 1105
AfaI GTAC 3 cut(s) 654, 829, 1250
AfiI CCNNNNNNNGG 6 cut(s) 319, 416, 799, 901, 1172, 1276
AhdI GACNNNNNGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 111, 933, 1145
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 8 cut(s) 31, 161, 221, 566, 914, 947, 1061, 1235
AluI AGCT 8 cut(s) 31, 161, 221, 566, 914, 947, 1061, 1235
Alw26I GTCTC 2 cut(s) 206, 1129
AlwI GGATC 1 cut(s) 925
Ama87I CYCGRG 1 cut(s) 1075
Aor13HI TCCGGA 1 cut(s) 1041
AoxI GGCC 3 cut(s) 936, 970, 1080
ApeKI GCWGC 7 cut(s) 158, 221, 224, 527, 566, 911, 1010
ApoI RAATTY 1 cut(s) 1105
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 1080
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 3 cut(s) 100, 172, 521
AvaI CYCGRG 1 cut(s) 1075
BalI TGGCCA 1 cut(s) 972
BbsI GAAGAC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 7 cut(s) 170, 208, 211, 514, 553, 923, 997
BccI CCATC 7 cut(s) 74, 187, 242, 324, 485, 659, 710
BciT130I CCWGG 3 cut(s) 113, 935, 1147
BciVI GTATCC 1 cut(s) 275
BcnI CCSGG 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 206, 1129
BfmI CTRYAG 1 cut(s) 794
BfuAI ACCTGC 3 cut(s) 132, 268, 1274
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 7 cut(s) 159, 222, 225, 528, 567, 912, 1011
BlsI GCNGC 7 cut(s) 160, 223, 226, 529, 568, 913, 1012
Bme1390I CCNGG 4 cut(s) 113, 418, 935, 1147
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 1 cut(s) 1075
BmgT120I GGNCC 1 cut(s) 1080
BmrFI CCNGG 4 cut(s) 113, 418, 935, 1147
BmsI GCATC 2 cut(s) 506, 1041
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 710
BpuMI CCSGG 1 cut(s) 418
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 111, 277, 818
BsaWI WCCGGW 1 cut(s) 1041
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 416, 799, 901, 1172, 1276
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 4 cut(s) 313, 467, 939, 1211
Bse3DI GCAATG 1 cut(s) 1120
BseAI TCCGGA 1 cut(s) 1041
BseBI CCWGG 3 cut(s) 113, 935, 1147
BseDI CCNNGG 3 cut(s) 111, 277, 818
BseGI GGATG 4 cut(s) 270, 309, 335, 632
BseLI CCNNNNNNNGG 6 cut(s) 319, 416, 799, 901, 1172, 1276
BseMI GCAATG 1 cut(s) 1120
BseMII CTCAG 1 cut(s) 724
BseNI ACTGG 4 cut(s) 313, 467, 939, 1211
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 7 cut(s) 170, 208, 211, 514, 553, 923, 997
BseYI CCCAGC 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 1219
BshFI GGCC 3 cut(s) 938, 972, 1082
BsiEI CGRYCG 1 cut(s) 1219
BsiHKCI CYCGRG 1 cut(s) 1075
BsiSI CCGG 3 cut(s) 417, 1042, 1083
BslI CCNNNNNNNGG 6 cut(s) 319, 416, 799, 901, 1172, 1276
BsmAI GTCTC 2 cut(s) 206, 1129
BsnI GGCC 3 cut(s) 938, 972, 1082
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 1075
Bsp13I TCCGGA 1 cut(s) 1041
Bsp143I GATC 2 cut(s) 930, 1216
BspACI CCGC 5 cut(s) 277, 279, 507, 687, 1068
BspANI GGCC 3 cut(s) 938, 972, 1082
BspCNI CTCAG 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 1041
BspFNI CGCG 1 cut(s) 279
BspMI ACCTGC 3 cut(s) 132, 268, 1274
BspPI GGATC 1 cut(s) 925
BspQI GCTCTTC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 1120
BsrFI RCCGGY 1 cut(s) 1082
BsrI ACTGG 4 cut(s) 313, 467, 939, 1211
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 3 cut(s) 111, 277, 818
BssMI GATC 2 cut(s) 930, 1216
Bst2UI CCWGG 3 cut(s) 113, 935, 1147
Bst4CI ACNGT 5 cut(s) 63, 302, 515, 652, 662
Bst6I CTCTTC 2 cut(s) 621, 775
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstC8I GCNNGC 2 cut(s) 981, 985
BstDEI CTNAG 2 cut(s) 244, 710
BstDSI CCRYGG 2 cut(s) 277, 818
BstF5I GGATG 4 cut(s) 270, 309, 335, 632
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 2 cut(s) 933, 1219
BstMAI GTCTC 2 cut(s) 206, 1129
BstMBI GATC 2 cut(s) 930, 1216
BstMCI CGRYCG 1 cut(s) 1219
BstMWI GCNNNNNNNGC 4 cut(s) 233, 611, 944, 1007
BstNI CCWGG 3 cut(s) 113, 935, 1147
BstNSI RCATGY 2 cut(s) 983, 987
BstSCI CCNGG 4 cut(s) 111, 416, 933, 1145
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 7 cut(s) 170, 208, 211, 514, 553, 923, 997
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 930
BstXI CCANNNNNNTGG 1 cut(s) 665
BstYI RGATCY 1 cut(s) 930
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 3 cut(s) 938, 972, 1082
BtgI CCRYGG 2 cut(s) 277, 818
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 4 cut(s) 270, 309, 335, 632
BtsIMutI CAGTG 1 cut(s) 658
BveI ACCTGC 3 cut(s) 132, 268, 1274
Cac8I GCNNGC 2 cut(s) 981, 985
CfoI GCGC 1 cut(s) 759
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 1 cut(s) 1080
Cfr42I CCGCGG 1 cut(s) 280
CseI GACGC 1 cut(s) 743
CsiI ACCWGGT 1 cut(s) 1145
Csp6I GTAC 3 cut(s) 653, 828, 1249
CviAII CATG 5 cut(s) 25, 924, 980, 984, 1014
CviQI GTAC 3 cut(s) 653, 828, 1249
DdeI CTNAG 2 cut(s) 244, 710
DpnI GATC 2 cut(s) 932, 1218
DpnII GATC 2 cut(s) 930, 1216
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 2 cut(s) 621, 775
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 2 cut(s) 621, 775
Eco31I GGTCTC 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 1075
EcoRII CCWGG 3 cut(s) 111, 933, 1145
EcoT22I ATGCAT 1 cut(s) 985
FaeI CATG 5 cut(s) 28, 927, 983, 987, 1017
FatI CATG 5 cut(s) 24, 923, 979, 983, 1013
FauI CCCGC 1 cut(s) 694
Fnu4HI GCNGC 7 cut(s) 159, 222, 225, 528, 567, 912, 1011
FokI GGATG 4 cut(s) 257, 296, 342, 639
Fsp4HI GCNGC 7 cut(s) 159, 222, 225, 528, 567, 912, 1011
GlaI GCGC 1 cut(s) 758
GluI GCNGC 7 cut(s) 159, 222, 225, 528, 567, 912, 1011
GsaI CCCAGC 1 cut(s) 868
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 3 cut(s) 938, 972, 1082
HapII CCGG 3 cut(s) 417, 1042, 1083
HgaI GACGC 1 cut(s) 743
HhaI GCGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 28, 927, 983, 987, 1017
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 4 cut(s) 811, 889, 1045, 1071
HpaII CCGG 3 cut(s) 417, 1042, 1083
HphI GGTGA 3 cut(s) 100, 172, 521
Hpy188I TCNGA 2 cut(s) 347, 1050
Hpy188III TCNNGA 7 cut(s) 291, 559, 624, 731, 886, 1042, 1075
Hpy99I CGWCG 1 cut(s) 378
HpyCH4III ACNGT 5 cut(s) 63, 302, 515, 652, 662
HpyCH4IV ACGT 1 cut(s) 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 611, 944, 1007
HpyF3I CTNAG 2 cut(s) 244, 710
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 5 cut(s) 28, 927, 983, 987, 1017
HspAI GCGC 1 cut(s) 757
Kpn2I TCCGGA 1 cut(s) 1041
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 2 cut(s) 930, 1216
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 4 cut(s) 218, 233, 293, 952
Lsp1109I GCAGC 7 cut(s) 170, 208, 211, 514, 553, 923, 997
LweI GCATC 2 cut(s) 506, 1041
MabI ACCWGGT 1 cut(s) 1145
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 4 cut(s) 57, 106, 509, 1256
MalI GATC 2 cut(s) 932, 1218
MboI GATC 2 cut(s) 930, 1216
MboII GAAGA 4 cut(s) 84, 587, 608, 792
MfeI CAATTG 2 cut(s) 533, 738
MflI RGATCY 1 cut(s) 930
MlsI TGGCCA 1 cut(s) 972
MluCI AATT 7 cut(s) 349, 533, 738, 873, 1003, 1020, 1105
MluNI TGGCCA 1 cut(s) 972
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 2 cut(s) 297, 1180
MnlI CCTC 9 cut(s) 313, 317, 320, 517, 539, 719, 848, 1071, 1173
Mox20I TGGCCA 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 985
MroI TCCGGA 1 cut(s) 1041
MscI TGGCCA 1 cut(s) 972
MseI TTAA 4 cut(s) 474, 872, 1023, 1284
MslI CAYNNNNRTG 2 cut(s) 663, 978
Msp20I TGGCCA 1 cut(s) 972
MspA1I CMGCKG 2 cut(s) 279, 507
MspI CCGG 3 cut(s) 417, 1042, 1083
MspR9I CCNGG 4 cut(s) 113, 418, 935, 1147
MunI CAATTG 2 cut(s) 533, 738
MvaI CCWGG 3 cut(s) 113, 935, 1147
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 4 cut(s) 233, 611, 944, 1007
NciI CCSGG 1 cut(s) 418
NdeII GATC 2 cut(s) 930, 1216
NlaIII CATG 5 cut(s) 28, 927, 983, 987, 1017
NmuCI GTSAC 3 cut(s) 106, 509, 1256
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 2 cut(s) 983, 987
PaeI GCATGC 2 cut(s) 983, 987
PaeR7I CTCGAG 1 cut(s) 1075
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PfeI GAWTC 3 cut(s) 811, 1045, 1071
PflMI CCANNNNNTGG 1 cut(s) 1276
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 7 cut(s) 160, 223, 226, 529, 568, 913, 1012
Ple19I CGATCG 1 cut(s) 1219
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Psp6I CCWGG 3 cut(s) 111, 933, 1145
PspFI CCCAGC 1 cut(s) 864
PspGI CCWGG 3 cut(s) 111, 933, 1145
PspPI GGNCC 1 cut(s) 1080
PsuI RGATCY 1 cut(s) 930
PvuI CGATCG 1 cut(s) 1219
RsaI GTAC 3 cut(s) 654, 829, 1250
RsaNI GTAC 3 cut(s) 653, 828, 1249
RseI CAYNNNNRTG 2 cut(s) 663, 978
SacII CCGCGG 1 cut(s) 280
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 4 cut(s) 474, 872, 1023, 1284
SatI GCNGC 7 cut(s) 159, 222, 225, 528, 567, 912, 1011
Sau3AI GATC 2 cut(s) 930, 1216
Sau96I GGNCC 1 cut(s) 1080
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 4 cut(s) 113, 418, 935, 1147
SexAI ACCWGGT 1 cut(s) 1145
SfaNI GCATC 2 cut(s) 506, 1041
SfcI CTRYAG 1 cut(s) 794
Sfr274I CTCGAG 1 cut(s) 1075
Sfr303I CCGCGG 1 cut(s) 280
SgrBI CCGCGG 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 1075
SmiMI CAYNNNNRTG 2 cut(s) 663, 978
SmlI CTYRAG 1 cut(s) 1075
SmoI CTYRAG 1 cut(s) 1075
SphI GCATGC 2 cut(s) 983, 987
Sse9I AATT 7 cut(s) 349, 533, 738, 873, 1003, 1020, 1105
SsiI CCGC 5 cut(s) 277, 279, 507, 687, 1068
StyD4I CCNGG 4 cut(s) 111, 416, 933, 1145
TaaI ACNGT 5 cut(s) 63, 302, 515, 652, 662
TaiI ACGT 1 cut(s) 556
TaqI TCGA 4 cut(s) 481, 544, 1076, 1215
TaqII GACCGA 1 cut(s) 1145
TasI AATT 7 cut(s) 349, 533, 738, 873, 1003, 1020, 1105
TatI WGTACW 1 cut(s) 652
TfiI GAWTC 3 cut(s) 811, 1045, 1071
Tru1I TTAA 4 cut(s) 474, 872, 1023, 1284
Tru9I TTAA 4 cut(s) 474, 872, 1023, 1284
TscAI CASTG 1 cut(s) 665
TseFI GTSAC 3 cut(s) 106, 509, 1256
TseI GCWGC 7 cut(s) 158, 221, 224, 527, 566, 911, 1010
Tsp45I GTSAC 3 cut(s) 106, 509, 1256
TspDTI ATGAA 3 cut(s) 41, 372, 1090
TspGWI ACGGA 2 cut(s) 835, 1236
TspRI CASTG 1 cut(s) 665
Van91I CCANNNNNTGG 1 cut(s) 1276
XapI RAATTY 1 cut(s) 1105
XceI RCATGY 2 cut(s) 983, 987
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 1075
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.