pycom16g12010

Pentatricopeptide repeat-containing protein At5g66520-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013694
Physical Location & Seq
Forward (+)
8390234 .. 8392120
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1887 bp
ATGACTTCGCTGCAAGTCCTTCAACCCACCGGGCCCACCCCACCCTCCCTAAGTTCGCCCAGGACCCACATTTCACCGTTACGCGGCCTGGAATCATGCTCTACCATGGCGGAGCTCAAGCAGTTGCACTCCCAAGTCATCAGGCTCGGCCTCGCCACCGACAACGACGCTATGGGCCGGGTCATCAAGTTCTGCGCCCTCTCCAAAAATGGCGATTTGGGTTATGCCCTCCAGGTGTTCGACGCAATGCCTCAACCGGACGCATTTATTTACAACACCGTAATGAGAGGTTACTTGCAGTGCCAATTGCCGAGAGATTGCATTGTTTTGTACTCGCAGATGTTGCAGGACTTTGTGACTCCAAATAGGTTCACGTTTCCGTCTGTTGTACGAGCTTGTTGCGCTGATGGTGCTGTTGCAGAAGGGAAACAAGTCCATGCCCATGTCGTTAAACTCGGGTTTGGAGATGATGGGTTTTGTCAGAACAATTTGATTCACATGTATGTGAAGTTTCAGTCTTTAGAGGAAGCTAGAAGGGTGTTTGACAAGATGCCCCGGGTGGATGTCGTGTCTTGGACGACTTTGATTACTGGGTACTCCCAGTGGGGCTTTGTCGACGAGGCTTTTGAAATATTTGAGTTGATGCCCGAGAAGAATTCCGTATCTTGGAATGCAATGATTTCCTCTTATGTCCAGAGTGATCGTTTTCATGAGGCGTTTGCTTTGTTTCAAAGAATGCGCGTGGAAAACGTAGAATTGGATAAGTTCATGGCGGCTAGTATGTTATCAGCTTGTACAGGTTTAGGTGCTCTAGAACAAGGGAAGTGGATACATGGATATATTGAGAAAAGTGGGATTGAATTGGACTCAAAACTTGCAACAACTATCATTGACATGTATTGCAAATGTGGCTGCCTGGAGAAGGCTTTTGAAGTTTTCAATGGTTTGCCCCATAAAGGGATTTCTTCATGGAATTGCATGATTGGAGGGCTAGCAATGCACGGGAAAGGAGAGGCAGCCGTTGAGCTTTTCGAGCAGATGCAAAGGGACATGGTAGCTCCCGACAACATTACATTTGTTAATGTTCTTAGTGCATGTGCCCACTCGGGGTTAGTAGAAAAGGGGCAGCAGTACTTCCGCAGCATGGTTGAAGTCCACGGTATTGAGCCCAGAACAGAGCATTTTGGCTGCATGGTTGATCTTCTTGGAAGAGCCGGGAGGCTGGAGGAAGCAAGAAAGCTAATAAACGAGATGCCTATGAGCCCCGATGTAGGTGTCTTGGGTGCTCTCCTCGGAGCTTGTAAGATCCATGGAAATGTTGAACTGGGAGACGGAATAGGGAGGAGGGTAATCGAACTGGAACCAGAAAATAGCGGGCGCTATGTGTTGTTAGCCAATCTATACGCAAATGCGGGCAGATGGGACGACGTTGCCAATGTGAGGAGGTTGATGAATGACAGAGGAGTGAAGAAGGTTCCTGGATTTTCCATGATTGAATTGGAAGGCACTGTCAGTGAATTCATTGCCGGTGGAGGGTCTCATCCTCAGACAAAAGAGATATACTCGAAAGTGGATGAAATGTTGAAATGTATAAGATCTGCAGGATATGTTCCGGACACGGAGGGAGTGTTGCATGATCTCGACGAGGAGGAAAGAGAGAACCCTTTATACTACCACAGTGAGAAACTTGCAATTGCATTTGGCCTGTTGAAGACAAAACCCAGGGAAACTCTTCGTATATCGAAGAATTTACGAGTCTGCAAAGACTGCCACCAGGCAAGCAAGCTCTGGATCTCGAAGGTTTTCGACCGCGAAATAATCGTGAGGGATAGAAATCGCTTCCACCATTTTAAAGGGGGAGAGTGTTCTTGTAAAGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

629

Amino Acids

70.35

Weight (kDa)

6.12

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 134 8.3e-09 PPR repeat family
PPR PF01535 190 - 219 3.8e-08 PPR repeat
PPR_2 PF13041 218 - 265 2.8e-08 PPR repeat family
PPR PF01535 221 - 250 1e-07 PPR repeat
PPR_2 PF13041 321 - 368 7.2e-11 PPR repeat family
PPR PF01535 323 - 350 7.3e-07 PPR repeat
E_motif PF20431 437 - 499 6.3e-20 E motif
DYW_deaminase PF14432 535 - 628 7.4e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 615
AccII CGCG 3 cut(s) 84, 741, 1812
AccIII TCCGGA 1 cut(s) 1612
AciI CCGC 7 cut(s) 84, 110, 773, 1138, 1374, 1412, 1810
AclWI GGATC 2 cut(s) 1300, 1799
AcsI RAATTY 3 cut(s) 655, 1517, 1747
AfaI GTAC 5 cut(s) 332, 390, 596, 796, 1133
AflIII ACRYGT 2 cut(s) 498, 894
AjnI CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
AluBI AGCT 9 cut(s) 115, 395, 530, 791, 1027, 1058, 1240, 1298, 1786
AluI AGCT 9 cut(s) 115, 395, 530, 791, 1027, 1058, 1240, 1298, 1786
Alw21I GWGCWC 3 cut(s) 117, 811, 1288
Alw26I GTCTC 2 cut(s) 1323, 1542
AlwI GGATC 2 cut(s) 1300, 1799
Ama87I CYCGRG 4 cut(s) 455, 555, 647, 1105
Aor13HI TCCGGA 1 cut(s) 1612
AoxI GGCC 5 cut(s) 32, 85, 148, 175, 1702
ApaI GGGCCC 1 cut(s) 36
ApeKI GCWGC 6 cut(s) 10, 912, 1016, 1126, 1140, 1188
ApoI RAATTY 3 cut(s) 655, 1517, 1747
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 2 cut(s) 1731, 1802
AspLEI GCGC 4 cut(s) 197, 404, 741, 1380
AspS9I GGNCC 4 cut(s) 32, 33, 63, 175
AsuC2I CCSGG 5 cut(s) 31, 179, 556, 557, 1216
AsuHPI GGTGA 1 cut(s) 66
AsuNHI GCTAGC 1 cut(s) 991
AvaI CYCGRG 4 cut(s) 455, 555, 647, 1105
AvaII GGWCC 1 cut(s) 63
BaeGI GKGCMC 2 cut(s) 36, 1102
BanII GRGCYC 4 cut(s) 36, 117, 1170, 1265
BbsI GAAGAC 1 cut(s) 1718
Bbv12I GWGCWC 3 cut(s) 117, 811, 1288
BbvI GCAGC 5 cut(s) 899, 1028, 1138, 1152, 1175
BccI CCATC 3 cut(s) 401, 464, 1413
BceAI ACGGC 1 cut(s) 1004
BcgI CGANNNNNNTGC 2 cut(s) 381, 415
BciT130I CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 5 cut(s) 31, 179, 556, 557, 1216
BcoDI GTCTC 2 cut(s) 1323, 1542
BfaI CTAG 4 cut(s) 531, 777, 812, 992
BfmI CTRYAG 1 cut(s) 1599
BfoI RGCGCY 1 cut(s) 1381
BfuI GTATCC 1 cut(s) 822
BglII AGATCT 1 cut(s) 1595
BisI GCNGC 8 cut(s) 11, 85, 774, 913, 1017, 1127, 1141, 1189
BlsI GCNGC 8 cut(s) 12, 86, 775, 914, 1018, 1128, 1142, 1190
BmcAI AGTACT 1 cut(s) 1133
Bme18I GGWCC 1 cut(s) 63
BmeT110I CYCGRG 4 cut(s) 455, 555, 647, 1105
BmgT120I GGNCC 4 cut(s) 32, 33, 63, 175
BmiI GGNNCC 4 cut(s) 34, 65, 1362, 1476
BmrI ACTGGG 3 cut(s) 595, 600, 1334
BmsI GCATC 4 cut(s) 540, 633, 1029, 1242
BmtI GCTAGC 1 cut(s) 995
BmuI ACTGGG 3 cut(s) 595, 600, 1334
BpiI GAAGAC 1 cut(s) 1718
BplI GAGNNNNNCTC 2 cut(s) 1547, 1579
BpmI CTGGAG 3 cut(s) 215, 938, 1244
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 5 cut(s) 31, 179, 556, 557, 1216
BsaBI GATNNNNATC 1 cut(s) 1833
BsaI GGTCTC 1 cut(s) 1542
BsaJI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BsaWI WCCGGW 2 cut(s) 256, 1612
BsaXI ACNNNNNCTCC 2 cut(s) 1613, 1643
Bse118I RCCGGY 1 cut(s) 1526
Bse1I ACTGG 5 cut(s) 595, 601, 1329, 1362, 1886
Bse3DI GCAATG 4 cut(s) 252, 681, 1002, 1521
Bse8I GATNNNNATC 1 cut(s) 1833
BseAI TCCGGA 1 cut(s) 1612
BseBI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BseDI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BseGI GGATG 3 cut(s) 568, 1540, 1579
BseJI GATNNNNATC 1 cut(s) 1833
BseMI GCAATG 4 cut(s) 252, 681, 1002, 1521
BseMII CTCAG 1 cut(s) 1559
BseNI ACTGG 5 cut(s) 595, 601, 1329, 1362, 1886
BseRI GAGGAG 5 cut(s) 1280, 1357, 1456, 1476, 1661
BseSI GKGCMC 2 cut(s) 36, 1102
BseXI GCAGC 5 cut(s) 899, 1028, 1138, 1152, 1175
Bsh1236I CGCG 3 cut(s) 84, 741, 1812
Bsh1285I CGRYCG 1 cut(s) 1810
BshFI GGCC 5 cut(s) 34, 87, 150, 177, 1704
BsiEI CGRYCG 1 cut(s) 1810
BsiHKAI GWGCWC 3 cut(s) 117, 811, 1288
BsiHKCI CYCGRG 4 cut(s) 455, 555, 647, 1105
BsiSI CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
BslFI GGGAC 2 cut(s) 1061, 1436
BsmAI GTCTC 2 cut(s) 1323, 1542
BsmBI CGTCTC 1 cut(s) 1323
BsmFI GGGAC 2 cut(s) 1061, 1436
BsmI GAATGC 2 cut(s) 676, 741
BsnI GGCC 5 cut(s) 34, 87, 150, 177, 1704
Bso31I GGTCTC 1 cut(s) 1542
BsoBI CYCGRG 4 cut(s) 455, 555, 647, 1105
Bsp120I GGGCCC 1 cut(s) 32
Bsp1286I GDGCHC 7 cut(s) 36, 117, 811, 1102, 1170, 1265, 1288
Bsp13I TCCGGA 1 cut(s) 1612
Bsp1407I TGTACA 1 cut(s) 794
Bsp143I GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
Bsp19I CCATGG 2 cut(s) 105, 1309
BspACI CCGC 7 cut(s) 84, 110, 773, 1138, 1374, 1412, 1810
BspANI GGCC 5 cut(s) 34, 87, 150, 177, 1704
BspCNI CTCAG 1 cut(s) 1558
BspEI TCCGGA 1 cut(s) 1612
BspFNI CGCG 3 cut(s) 84, 741, 1812
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 4 cut(s) 34, 65, 1362, 1476
BspMAI CTGCAG 1 cut(s) 1603
BspOI GCTAGC 1 cut(s) 995
BspPI GGATC 2 cut(s) 1300, 1799
BspQI GCTCTTC 1 cut(s) 1204
BspTNI GGTCTC 1 cut(s) 1542
BsrDI GCAATG 4 cut(s) 252, 681, 1002, 1521
BsrFI RCCGGY 1 cut(s) 1526
BsrGI TGTACA 1 cut(s) 794
BsrI ACTGG 5 cut(s) 595, 601, 1329, 1362, 1886
BssAI RCCGGY 1 cut(s) 1526
BssECI CCNNGG 9 cut(s) 59, 105, 554, 555, 1156, 1291, 1309, 1721, 1722
BssMI GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
BssT1I CCWWGG 2 cut(s) 105, 1309
Bst2UI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
Bst4CI ACNGT 5 cut(s) 78, 280, 1160, 1510, 1679
Bst6I CTCTTC 2 cut(s) 1204, 1737
BstAPI GCANNNNNTGC 2 cut(s) 343, 1767
BstAUI TGTACA 1 cut(s) 794
BstC8I GCNNGC 5 cut(s) 993, 1376, 1414, 1780, 1784
BstDEI CTNAG 3 cut(s) 50, 1088, 1545
BstDSI CCRYGG 3 cut(s) 105, 1156, 1309
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 3 cut(s) 568, 1540, 1579
BstFNI CGCG 3 cut(s) 84, 741, 1812
BstH2I RGCGCY 1 cut(s) 1381
BstHHI GCGC 4 cut(s) 197, 404, 741, 1380
BstKTI GATC 6 cut(s) 703, 1201, 1308, 1598, 1639, 1794
BstMAI GTCTC 2 cut(s) 1323, 1542
BstMBI GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
BstMCI CGRYCG 1 cut(s) 1810
BstMWI GCNNNNNNNGC 7 cut(s) 343, 401, 410, 909, 997, 1033, 1767
BstNI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
BstNSI RCATGY 3 cut(s) 502, 898, 1098
BstSFI CTRYAG 1 cut(s) 1599
BstSLI GKGCMC 2 cut(s) 36, 1102
BstUI CGCG 3 cut(s) 84, 741, 1812
BstV1I GCAGC 5 cut(s) 899, 1028, 1138, 1152, 1175
BstV2I GAAGAC 1 cut(s) 1718
BstX2I RGATCY 3 cut(s) 1305, 1595, 1791
BstYI RGATCY 3 cut(s) 1305, 1595, 1791
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 5 cut(s) 34, 87, 150, 177, 1704
BtgI CCRYGG 3 cut(s) 105, 1156, 1309
BtsCI GGATG 3 cut(s) 568, 1540, 1579
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 5 cut(s) 305, 608, 1506, 1519, 1684
Cac8I GCNNGC 5 cut(s) 993, 1376, 1414, 1780, 1784
CciI TCATGA 1 cut(s) 709
CfoI GCGC 4 cut(s) 197, 404, 741, 1380
Cfr10I RCCGGY 1 cut(s) 1526
Cfr13I GGNCC 4 cut(s) 32, 33, 63, 175
Cfr9I CCCGGG 1 cut(s) 555
CseI GACGC 3 cut(s) 176, 251, 269
Csp6I GTAC 5 cut(s) 331, 389, 595, 795, 1132
CspCI CAANNNNNGTGG 2 cut(s) 806, 841
CviQI GTAC 5 cut(s) 331, 389, 595, 795, 1132
DdeI CTNAG 3 cut(s) 50, 1088, 1545
DpnI GATC 6 cut(s) 702, 1200, 1307, 1597, 1638, 1793
DpnII GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
DraI TTTAAA 1 cut(s) 1852
Eam1104I CTCTTC 2 cut(s) 1204, 1737
EarI CTCTTC 2 cut(s) 1204, 1737
EciI GGCGGA 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 105, 1309
Eco24I GRGCYC 4 cut(s) 36, 117, 1170, 1265
Eco31I GGTCTC 1 cut(s) 1542
Eco47I GGWCC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 115
Eco88I CYCGRG 4 cut(s) 455, 555, 647, 1105
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoO109I RGGNCCY 1 cut(s) 63
EcoRI GAATTC 2 cut(s) 655, 1517
EcoRII CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
EcoT14I CCWWGG 2 cut(s) 105, 1309
EcoT38I GRGCYC 4 cut(s) 36, 117, 1170, 1265
ErhI CCWWGG 2 cut(s) 105, 1309
Esp3I CGTCTC 1 cut(s) 1323
FaqI GGGAC 2 cut(s) 1061, 1436
FauI CCCGC 2 cut(s) 1367, 1405
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 8 cut(s) 11, 85, 774, 913, 1017, 1127, 1141, 1189
FokI GGATG 3 cut(s) 575, 1527, 1586
FriOI GRGCYC 4 cut(s) 36, 117, 1170, 1265
Fsp4HI GCNGC 8 cut(s) 11, 85, 774, 913, 1017, 1127, 1141, 1189
FspBI CTAG 4 cut(s) 531, 777, 812, 992
GlaI GCGC 4 cut(s) 196, 403, 740, 1379
GluI GCNGC 8 cut(s) 11, 85, 774, 913, 1017, 1127, 1141, 1189
GsuI CTGGAG 3 cut(s) 215, 938, 1244
HaeII RGCGCY 1 cut(s) 1381
HaeIII GGCC 5 cut(s) 34, 87, 150, 177, 1704
HapII CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
HgaI GACGC 3 cut(s) 176, 251, 269
HhaI GCGC 4 cut(s) 197, 404, 741, 1380
Hin6I GCGC 4 cut(s) 195, 402, 739, 1378
HinP1I GCGC 4 cut(s) 195, 402, 739, 1378
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HinfI GANTC 5 cut(s) 92, 358, 493, 866, 1755
HpaII CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 372, 616, 1156
Hpy188I TCNGA 3 cut(s) 483, 1295, 1548
Hpy188III TCNNGA 9 cut(s) 694, 710, 812, 1061, 1613, 1640, 1789, 1795, 1822
Hpy8I GTNNAC 3 cut(s) 372, 616, 1156
Hpy99I CGWCG 5 cut(s) 170, 245, 620, 1430, 1646
HpyAV CCTTC 7 cut(s) 29, 416, 528, 916, 1465, 1496, 1792
HpyCH4III ACNGT 5 cut(s) 78, 280, 1160, 1510, 1679
HpyCH4IV ACGT 3 cut(s) 374, 750, 1428
HpyF10VI GCNNNNNNNGC 7 cut(s) 343, 401, 410, 909, 997, 1033, 1767
HpyF3I CTNAG 3 cut(s) 50, 1088, 1545
HpySE526I ACGT 3 cut(s) 374, 750, 1428
HspAI GCGC 4 cut(s) 195, 402, 739, 1378
Kpn2I TCCGGA 1 cut(s) 1612
Kzo9I GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
LguI GCTCTTC 1 cut(s) 1204
LmnI GCTCC 3 cut(s) 112, 1063, 1295
Lsp1109I GCAGC 5 cut(s) 899, 1028, 1138, 1152, 1175
LweI GCATC 4 cut(s) 540, 633, 1029, 1242
MaeI CTAG 4 cut(s) 531, 777, 812, 992
MaeII ACGT 3 cut(s) 374, 750, 1428
MaeIII GTNAC 3 cut(s) 78, 290, 355
MalI GATC 6 cut(s) 702, 1200, 1307, 1597, 1638, 1793
MboI GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
MboII GAAGA 8 cut(s) 664, 957, 1193, 1221, 1480, 1723, 1724, 1756
MfeI CAATTG 2 cut(s) 305, 1692
MflI RGATCY 3 cut(s) 1305, 1595, 1791
MhlI GDGCHC 7 cut(s) 36, 117, 811, 1102, 1170, 1265, 1288
MlyI GAGTC 3 cut(s) 352, 860, 1764
MroI TCCGGA 1 cut(s) 1612
MroXI GAANNNNTTC 2 cut(s) 1731, 1802
MseI TTAA 3 cut(s) 450, 1080, 1851
MslI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
MspI CCGG 7 cut(s) 30, 178, 257, 556, 1215, 1527, 1613
MunI CAATTG 2 cut(s) 305, 1692
Mva1269I GAATGC 2 cut(s) 676, 741
MvaI CCWGG 7 cut(s) 61, 89, 233, 917, 1479, 1723, 1775
MvnI CGCG 3 cut(s) 84, 741, 1812
MwoI GCNNNNNNNGC 7 cut(s) 343, 401, 410, 909, 997, 1033, 1767
NciI CCSGG 5 cut(s) 31, 179, 556, 557, 1216
NcoI CCATGG 2 cut(s) 105, 1309
NdeII GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
NheI GCTAGC 1 cut(s) 991
NlaIV GGNNCC 4 cut(s) 34, 65, 1362, 1476
NmeAIII GCCGAG 2 cut(s) 126, 336
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 3 cut(s) 502, 898, 1098
PagI TCATGA 1 cut(s) 709
PasI CCCWGGG 1 cut(s) 1722
PciI ACATGT 2 cut(s) 498, 894
PciSI GCTCTTC 1 cut(s) 1204
PctI GAATGC 2 cut(s) 676, 741
PdmI GAANNNNTTC 2 cut(s) 1731, 1802
PfeI GAWTC 2 cut(s) 92, 493
PfoI TCCNGGA 1 cut(s) 1477
PkrI GCNGC 8 cut(s) 12, 86, 775, 914, 1018, 1128, 1142, 1190
PleI GAGTC 3 cut(s) 352, 860, 1763
PpsI GAGTC 3 cut(s) 352, 860, 1763
PpuMI RGGWCCY 1 cut(s) 63
PscI ACATGT 2 cut(s) 498, 894
Psp124BI GAGCTC 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
PspGI CCWGG 7 cut(s) 59, 87, 231, 915, 1477, 1721, 1773
PspN4I GGNNCC 4 cut(s) 34, 65, 1362, 1476
PspOMI GGGCCC 1 cut(s) 32
PspPI GGNCC 4 cut(s) 32, 33, 63, 175
PspPPI RGGWCCY 1 cut(s) 63
PsrI GAACNNNNNNTAC 2 cut(s) 1652, 1684
PstI CTGCAG 1 cut(s) 1603
PsuI RGATCY 3 cut(s) 1305, 1595, 1791
RsaI GTAC 5 cut(s) 332, 390, 596, 796, 1133
RsaNI GTAC 5 cut(s) 331, 389, 595, 795, 1132
RseI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 614
SapI GCTCTTC 1 cut(s) 1204
SaqAI TTAA 3 cut(s) 450, 1080, 1851
SatI GCNGC 8 cut(s) 11, 85, 774, 913, 1017, 1127, 1141, 1189
Sau3AI GATC 6 cut(s) 700, 1198, 1305, 1595, 1636, 1791
Sau96I GGNCC 4 cut(s) 32, 33, 63, 175
ScaI AGTACT 1 cut(s) 1133
SchI GAGTC 3 cut(s) 352, 860, 1764
SduI GDGCHC 7 cut(s) 36, 117, 811, 1102, 1170, 1265, 1288
SfaNI GCATC 4 cut(s) 540, 633, 1029, 1242
SfcI CTRYAG 1 cut(s) 1599
SinI GGWCC 1 cut(s) 63
SmaI CCCGGG 1 cut(s) 557
SmiMI CAYNNNNRTG 6 cut(s) 281, 441, 501, 503, 893, 1314
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SsiI CCGC 7 cut(s) 84, 110, 773, 1138, 1374, 1412, 1810
SspI AATATT 1 cut(s) 633
SspMI CTAG 4 cut(s) 531, 777, 812, 992
SstI GAGCTC 1 cut(s) 117
StyI CCWWGG 2 cut(s) 105, 1309
TaaI ACNGT 5 cut(s) 78, 280, 1160, 1510, 1679
TaiI ACGT 3 cut(s) 377, 753, 1431
TaqI TCGA 9 cut(s) 240, 615, 1032, 1353, 1565, 1641, 1742, 1796, 1806
TatI WGTACW 3 cut(s) 330, 794, 1131
TauI GCSGC 2 cut(s) 87, 776
TfiI GAWTC 2 cut(s) 92, 493
Tru1I TTAA 3 cut(s) 450, 1080, 1851
Tru9I TTAA 3 cut(s) 450, 1080, 1851
TscAI CASTG 5 cut(s) 305, 608, 1513, 1519, 1684
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 6 cut(s) 10, 912, 1016, 1126, 1140, 1188
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 6 cut(s) 698, 757, 957, 1466, 1510, 1590
TspGWI ACGGA 4 cut(s) 369, 649, 1347, 1634
TspMI CCCGGG 1 cut(s) 555
TspRI CASTG 5 cut(s) 305, 608, 1513, 1519, 1684
VpaK11BI GGWCC 1 cut(s) 63
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 3 cut(s) 655, 1517, 1747
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 3 cut(s) 502, 898, 1098
XcmI CCANNNNNNNNNTGG 1 cut(s) 1495
XmaI CCCGGG 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 2 cut(s) 1731, 1802
XspI CTAG 4 cut(s) 531, 777, 812, 992
ZrmI AGTACT 1 cut(s) 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.