Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007897

Physical Location & Seq

Genomic Coordinates Reverse (-)

10030461 .. 10033410

Gene Structure 2950 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGATGATCGCTAGGCGGCTCTGGCATAAGCGGACTTTGGTCAACGGTGGCCGGTGGCTCTGTCCGTACACCTCTTCAGCTCCGTCGCCGGCGGCGGTGCATTCTTCCCACCACTCTCCGTTTCGAGTCTCCTCTTCCGATCTCGTTGCGTTCTCTACTGCCGCTTCCACCTGGTTCTGCTTGGCCGATCAGGTATCCATTCGTAGAATTGCGTGTAATGTGAATGGGAGGTGGTTTTCAAGTGAGGCAGTGGCTGATGTTGCAGCAGGGGTTAATGGTAATGGTATAGTTGATGTACCGTTGGCTCAAACCGGAGAAGGCATTGCCGAATGCGAGCTACTCAAATGGTTTGTGCAGGAGGGGGATCAAGTTGACGAATTTCAGCCACTTTGTGAAGTTCAAAGTGACAAAGCAACCATCGAAATAACAAGCCGTTATCAAGGGAAAGTTTCTCAGCTTCTCTACATTCCTGGTGACATCGTCAAGGTTGGAGAAATTCTTTTAAAGATGGCCGTCGAGGAATCTCACGTTCCAAAGCAGATATTTGAGAATTTGGAAATCACTAAGTCACTAGATTCTGAGCTTAACACACATAACATAGGCGGAGTTTTGTCTACACCTCCTGTTCGGAGCCTTGCAAAGCAATATGATATAGATATAAATGAAGTCTCTGGAACCGGTAAAGATGGGAGAGTGTTACGAGAAGATGTCCTTAAATATGCTGCCCAGAAGGGAATCGTTGAAGTTCCTTCTGCCTCCTTGAGTGCTAGTCCTGAGAAAGCTTTGGGAAGAAAAGAGACTTGTGCACATACATCAGCTGAATCGGGATGGAATTATGAGGATACGACAGTTACCCTGAGGGGATTCCAGCGAAGAATGGTTACGTCAATGAGCATGGCTGCAAAAGTTCCACATTTTCATTATGTTGAGGATATAAAGTGTGATGCACTTGTGGAGCTGAAAAATTCTTTCCAAAGTAATAACACCGATTCCAATGTCAAGCACACTTTTCTTCCATTATTGATAAAGACTCTCTCAGTGGCCATGAGCAAATATCCGTTGATGAATAGTAGCTTCAATGAAGAGTCACTCGAGGTCATCCTCAAAGGTTCGCACAATATCGGAATTGCCATGGCTACTCCATATGGTCTGGTTGTGCCAATCATAAAGAACGTTCAATCTCTTTCCATTTTGGAGATAACAAAGGAACTTTCACGGTTACAACAATTGGCATTGGAAAACAAGCTCAAGCCTGAAGAGATATCTGGTGGAACAATAACTTTAAGCAACATTGGAGCAATCGGTGGGAAATATGGTTCGCCTCTTCTGAACTTGCCTGAGGTTGCCATTATTGCAATTGGGCGAATTCAGAAGGTTCCACGATTTGCAGATGACGGAGGTGTATATCCTGTCTCAGTTATGACGGTCAATATAGGCGCAGATCACAGAGTGCTGGATGGAGCAACTGTTGCCAGGTTCTGCAACGAGTGGAAACAATATATAGAGAATCCAGAGCTGCTCATGTTGCATATGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004147 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006950 GO:0007154 GO:0008150 GO:0008270 GO:0009267 GO:0009295 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009987 GO:0009991 GO:0010033 GO:0016407 GO:0016417 GO:0016740 GO:0016746 GO:0016747 GO:0019899 GO:0030062 GO:0030523 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0032991 GO:0033554 GO:0034285 GO:0042221 GO:0042594 GO:0042645 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043617 GO:0044389 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0070013 GO:0071496 GO:0098798 GO:1901700 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00280 ko00640 ko01100 ko01110 ko01130 map00280 map00640 map01100 map01110 map01130

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

513

Amino Acids

56.1

Weight (kDa)

6.15

Isoelectric Point (pI)

51.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	99 - 170	2.3e-17	Biotin-requiring enzyme
E3_binding	PF02817	203 - 238	2.7e-13	e3 binding domain
2-oxoacid_dh	PF00198	280 - 509	2.4e-80	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007897)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06850 AT3G06850
fragaria_vesca	FvH4_4g18561 FvH4_4g18561 FvH4_4g18561
malus_domestica	MD13G1165700.v1.1
prunus_persica	Prupe.1G132500_v2.0.a1
pyrus_communis	pycom13g14180 pycom16g14020
rosa_chinensis	RchiOBHm_Chr4g0423151
rosa_multiflora	Rmu_sc0000744.1_g000002 Rmu_sc0000744.1_g000004 Rmu_sc0008829.1_g000001 Rmu_sc0027756.1_g000002
rosa_roxburghii	Rroxscaffold_5G00365320
rosa_rugosa	Rorug04G0184700 Rorug04G0184800 Rorug04G0184800
rosa_samantha	Rh4AG244200 Rh4BG249400 Rh4CG258500 Rh4DG244100 Rh4DG244200
rosa_wichuraiana	Rw4G021040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	614
AciI	CCGC	6 cut(s)	16, 31, 92, 95, 162, 603
AclI	AACGTT	1 cut(s)	1173
AclWI	GGATC	1 cut(s)	372
AcoI	YGGCCR	4 cut(s)	49, 183, 510, 1041
AcsI	RAATTY	5 cut(s)	377, 495, 550, 964, 1365
AcuI	CTGAAG	2 cut(s)	60, 1275
AdeI	CACNNNGTG	2 cut(s)	392, 1450
AfaI	GTAC	2 cut(s)	68, 297
AgeI	ACCGGT	1 cut(s)	677
AgsI	TTSAA	5 cut(s)	240, 401, 743, 1078, 1178
AjnI	CCWGG	3 cut(s)	170, 469, 1472
AloI	GAACNNNNNNTCC	2 cut(s)	1300, 1332
Alw21I	GWGCWC	1 cut(s)	808
Alw26I	GTCTC	4 cut(s)	133, 673, 791, 1417
Alw44I	GTGCAC	1 cut(s)	804
AlwI	GGATC	1 cut(s)	372
AlwNI	CAGNNNCTG	2 cut(s)	254, 1479
Ama87I	CYCGRG	1 cut(s)	1091
AoxI	GGCC	4 cut(s)	49, 183, 510, 1041
ApaLI	GTGCAC	1 cut(s)	804
ApeKI	GCWGC	4 cut(s)	263, 722, 899, 1516
ApoI	RAATTY	5 cut(s)	377, 495, 550, 964, 1365
AsiGI	ACCGGT	1 cut(s)	677
AspLEI	GCGC	1 cut(s)	1439
AsuHPI	GGTGA	1 cut(s)	485
AvaI	CYCGRG	1 cut(s)	1091
AxyI	CCTNAGG	2 cut(s)	857, 1338
BaeGI	GKGCMC	1 cut(s)	808
BaeI	ACNNNNGTAYC	2 cut(s)	834, 867
BalI	TGGCCA	1 cut(s)	1043
BarI	GAAGNNNNNNTAC	2 cut(s)	865, 897
Bbv12I	GWGCWC	1 cut(s)	808
BbvI	GCAGC	4 cut(s)	275, 709, 886, 1503
BccI	CCATC	5 cut(s)	425, 502, 680, 822, 1451
BceAI	ACGGC	2 cut(s)	417, 497
BcgI	CGANNNNNNTGC	2 cut(s)	128, 162
BciT130I	CCWGG	3 cut(s)	172, 471, 1474
BciVI	GTATCC	2 cut(s)	205, 835
BcoDI	GTCTC	4 cut(s)	133, 673, 791, 1417
BfaI	CTAG	3 cut(s)	12, 572, 768
BfuI	GTATCC	2 cut(s)	205, 835
BisI	GCNGC	7 cut(s)	17, 93, 162, 264, 723, 900, 1517
BlsI	GCNGC	7 cut(s)	18, 94, 163, 265, 724, 901, 1518
Bme1390I	CCNGG	3 cut(s)	172, 471, 1474
BmeT110I	CYCGRG	1 cut(s)	1091
BmiI	GGNNCC	3 cut(s)	632, 676, 1377
BmrFI	CCNGG	3 cut(s)	172, 471, 1474
BmsI	GCATC	1 cut(s)	934
BoxI	GACNNNNGTC	1 cut(s)	38
BpuEI	CTTGAG	2 cut(s)	781, 1232
BsaJI	CCNNGG	1 cut(s)	1131
BsaWI	WCCGGW	2 cut(s)	311, 677
BsaXI	ACNNNNNCTCC	2 cut(s)	1452, 1482
Bse118I	RCCGGY	3 cut(s)	51, 88, 677
Bse21I	CCTNAGG	2 cut(s)	857, 1338
Bse3DI	GCAATG	1 cut(s)	321
BseBI	CCWGG	3 cut(s)	172, 471, 1474
BseDI	CCNNGG	1 cut(s)	1131
BseGI	GGATG	3 cut(s)	833, 1098, 1462
BseMI	GCAATG	1 cut(s)	321
BseMII	CTCAG	7 cut(s)	467, 570, 765, 848, 1050, 1329, 1428
BseRI	GAGGAG	1 cut(s)	121
BseSI	GKGCMC	1 cut(s)	808
BseXI	GCAGC	4 cut(s)	275, 709, 886, 1503
BsgI	GTGCAG	1 cut(s)	374
BshFI	GGCC	4 cut(s)	51, 185, 512, 1043
BshTI	ACCGGT	1 cut(s)	677
BsiHKAI	GWGCWC	1 cut(s)	808
BsiHKCI	CYCGRG	1 cut(s)	1091
BsiSI	CCGG	4 cut(s)	52, 89, 312, 678
BsmAI	GTCTC	4 cut(s)	133, 673, 791, 1417
BsmI	GAATGC	2 cut(s)	100, 335
BsnI	GGCC	4 cut(s)	51, 185, 512, 1043
BsoBI	CYCGRG	1 cut(s)	1091
Bsp1286I	GDGCHC	1 cut(s)	808
Bsp143I	GATC	5 cut(s)	6, 139, 187, 364, 1441
Bsp19I	CCATGG	1 cut(s)	1131
BspACI	CCGC	6 cut(s)	16, 31, 92, 95, 162, 603
BspANI	GGCC	4 cut(s)	51, 185, 512, 1043
BspCNI	CTCAG	7 cut(s)	466, 571, 766, 849, 1049, 1330, 1427
BspLI	GGNNCC	3 cut(s)	632, 676, 1377
BspPI	GGATC	1 cut(s)	372
BsrDI	GCAATG	1 cut(s)	321
BsrFI	RCCGGY	3 cut(s)	51, 88, 677
BssAI	RCCGGY	3 cut(s)	51, 88, 677
BssECI	CCNNGG	1 cut(s)	1131
BssMI	GATC	5 cut(s)	6, 139, 187, 364, 1441
BssT1I	CCWWGG	1 cut(s)	1131
Bst2UI	CCWGG	3 cut(s)	172, 471, 1474
Bst4CI	ACNGT	6 cut(s)	47, 300, 850, 1218, 1426, 1468
Bst6I	CTCTTC	5 cut(s)	79, 139, 1077, 1251, 1329
BstAPI	GCANNNNNTGC	1 cut(s)	1469
BstC8I	GCNNGC	2 cut(s)	90, 335
BstDEI	CTNAG	8 cut(s)	453, 564, 579, 774, 857, 1036, 1338, 1414
BstDSI	CCRYGG	1 cut(s)	1131
BstF5I	GGATG	3 cut(s)	833, 1098, 1462
BstHHI	GCGC	1 cut(s)	1439
BstKTI	GATC	5 cut(s)	9, 142, 190, 367, 1444
BstMAI	GTCTC	4 cut(s)	133, 673, 791, 1417
BstMBI	GATC	5 cut(s)	6, 139, 187, 364, 1441
BstMWI	GCNNNNNNNGC	5 cut(s)	22, 260, 1352, 1469, 1525
BstNI	CCWGG	3 cut(s)	172, 471, 1474
BstPAI	GACNNNNGTC	1 cut(s)	38
BstSCI	CCNGG	3 cut(s)	170, 469, 1472
BstSLI	GKGCMC	1 cut(s)	808
BstV1I	GCAGC	4 cut(s)	275, 709, 886, 1503
Bsu36I	CCTNAGG	2 cut(s)	857, 1338
BsuI	GTATCC	2 cut(s)	205, 835
BsuRI	GGCC	4 cut(s)	51, 185, 512, 1043
BtgI	CCRYGG	1 cut(s)	1131
BtsCI	GGATG	3 cut(s)	833, 1098, 1462
BtsI	GCAGTG	1 cut(s)	255
BtsIMutI	CAGTG	2 cut(s)	255, 1044
Cac8I	GCNNGC	2 cut(s)	90, 335
CaiI	CAGNNNCTG	2 cut(s)	254, 1479
CfoI	GCGC	1 cut(s)	1439
Cfr10I	RCCGGY	3 cut(s)	51, 88, 677
CsiI	ACCWGGT	1 cut(s)	170
Csp6I	GTAC	2 cut(s)	67, 296
CspAI	ACCGGT	1 cut(s)	677
CviAII	CATG	4 cut(s)	895, 1045, 1132, 1522
CviQI	GTAC	2 cut(s)	67, 296
DdeI	CTNAG	8 cut(s)	453, 564, 579, 774, 857, 1036, 1338, 1414
DpnI	GATC	5 cut(s)	8, 141, 189, 366, 1443
DpnII	GATC	5 cut(s)	6, 139, 187, 364, 1441
DraI	TTTAAA	1 cut(s)	504
DraIII	CACNNNGTG	2 cut(s)	392, 1450
EaeI	YGGCCR	4 cut(s)	49, 183, 510, 1041
Eam1104I	CTCTTC	5 cut(s)	79, 139, 1077, 1251, 1329
EarI	CTCTTC	5 cut(s)	79, 139, 1077, 1251, 1329
EciI	GGCGGA	1 cut(s)	618
Eco130I	CCWWGG	1 cut(s)	1131
Eco32I	GATATC	1 cut(s)	1263
Eco57I	CTGAAG	2 cut(s)	60, 1275
Eco81I	CCTNAGG	2 cut(s)	857, 1338
Eco88I	CYCGRG	1 cut(s)	1091
EcoRI	GAATTC	1 cut(s)	1365
EcoRII	CCWGG	3 cut(s)	170, 469, 1472
EcoRV	GATATC	1 cut(s)	1263
EcoT14I	CCWWGG	1 cut(s)	1131
ErhI	CCWWGG	1 cut(s)	1131
FaeI	CATG	4 cut(s)	898, 1048, 1135, 1525
FalI	AAGNNNNNCTT	2 cut(s)	696, 728
FatI	CATG	4 cut(s)	894, 1044, 1131, 1521
FauNDI	CATATG	2 cut(s)	1144, 1530
FblI	GTMKAC	1 cut(s)	614
Fnu4HI	GCNGC	7 cut(s)	17, 93, 162, 264, 723, 900, 1517
FokI	GGATG	3 cut(s)	840, 1085, 1469
Fsp4HI	GCNGC	7 cut(s)	17, 93, 162, 264, 723, 900, 1517
FspBI	CTAG	3 cut(s)	12, 572, 768
GlaI	GCGC	1 cut(s)	1438
GluI	GCNGC	7 cut(s)	17, 93, 162, 264, 723, 900, 1517
HaeIII	GGCC	4 cut(s)	51, 185, 512, 1043
HapII	CCGG	4 cut(s)	52, 89, 312, 678
HhaI	GCGC	1 cut(s)	1439
Hin1II	CATG	4 cut(s)	898, 1048, 1135, 1525
Hin6I	GCGC	1 cut(s)	1437
HinP1I	GCGC	1 cut(s)	1437
HincII	GTYRAC	2 cut(s)	43, 373
HindII	GTYRAC	2 cut(s)	43, 373
HindIII	AAGCTT	1 cut(s)	780
HpaII	CCGG	4 cut(s)	52, 89, 312, 678
HphI	GGTGA	1 cut(s)	485
Hpy166II	GTNNAC	5 cut(s)	43, 69, 373, 615, 806
Hpy188I	TCNGA	6 cut(s)	139, 580, 630, 1124, 1329, 1371
Hpy188III	TCNNGA	4 cut(s)	672, 773, 825, 1511
Hpy8I	GTNNAC	5 cut(s)	43, 69, 373, 615, 806
Hpy99I	CGWCG	2 cut(s)	88, 518
HpyAV	CCTTC	4 cut(s)	311, 724, 759, 1366
HpyCH4III	ACNGT	6 cut(s)	47, 300, 850, 1218, 1426, 1468
HpyCH4IV	ACGT	3 cut(s)	528, 884, 1173
HpyF10VI	GCNNNNNNNGC	5 cut(s)	22, 260, 1352, 1469, 1525
HpyF3I	CTNAG	8 cut(s)	453, 564, 579, 774, 857, 1036, 1338, 1414
HpySE526I	ACGT	3 cut(s)	528, 884, 1173
Hsp92II	CATG	4 cut(s)	898, 1048, 1135, 1525
HspAI	GCGC	1 cut(s)	1437
KroI	GCCGGC	1 cut(s)	88
KroNI	GCCGGC	1 cut(s)	90
Kzo9I	GATC	5 cut(s)	6, 139, 187, 364, 1441
LmnI	GCTCC	5 cut(s)	85, 630, 955, 1295, 1460
Lsp1109I	GCAGC	4 cut(s)	275, 709, 886, 1503
LweI	GCATC	1 cut(s)	934
MabI	ACCWGGT	1 cut(s)	170
MaeI	CTAG	3 cut(s)	12, 572, 768
MaeII	ACGT	3 cut(s)	528, 884, 1173
MaeIII	GTNAC	8 cut(s)	404, 473, 567, 696, 850, 880, 1086, 1218
MalI	GATC	5 cut(s)	8, 141, 189, 366, 1443
MboI	GATC	5 cut(s)	6, 139, 187, 364, 1441
MfeI	CAATTG	2 cut(s)	1226, 1356
MhlI	GDGCHC	1 cut(s)	808
MlsI	TGGCCA	1 cut(s)	1043
MluNI	TGGCCA	1 cut(s)	1043
MlyI	GAGTC	3 cut(s)	135, 1024, 1094
MmeI	TCCRAC	1 cut(s)	469
Mox20I	TGGCCA	1 cut(s)	1043
MreI	CGCCGGCG	1 cut(s)	88
MroNI	GCCGGC	1 cut(s)	88
MscI	TGGCCA	1 cut(s)	1043
MseI	TTAA	5 cut(s)	273, 503, 585, 714, 1283
Msp20I	TGGCCA	1 cut(s)	1043
MspA1I	CMGCKG	1 cut(s)	818
MspI	CCGG	4 cut(s)	52, 89, 312, 678
MspR9I	CCNGG	3 cut(s)	172, 471, 1474
MunI	CAATTG	2 cut(s)	1226, 1356
Mva1269I	GAATGC	2 cut(s)	100, 335
MvaI	CCWGG	3 cut(s)	172, 471, 1474
MwoI	GCNNNNNNNGC	5 cut(s)	22, 260, 1352, 1469, 1525
NaeI	GCCGGC	1 cut(s)	90
NcoI	CCATGG	1 cut(s)	1131
NdeI	CATATG	2 cut(s)	1144, 1530
NdeII	GATC	5 cut(s)	6, 139, 187, 364, 1441
NgoMIV	GCCGGC	1 cut(s)	88
NlaIII	CATG	4 cut(s)	898, 1048, 1135, 1525
NlaIV	GGNNCC	3 cut(s)	632, 676, 1377
NmuCI	GTSAC	4 cut(s)	404, 473, 567, 1086
PaeR7I	CTCGAG	1 cut(s)	1091
PctI	GAATGC	2 cut(s)	100, 335
PdiI	GCCGGC	1 cut(s)	90
PfeI	GAWTC	7 cut(s)	521, 575, 735, 821, 864, 989, 1507
PflFI	GACNNNGTC	1 cut(s)	479
PinAI	ACCGGT	1 cut(s)	677
PkrI	GCNGC	7 cut(s)	18, 94, 163, 265, 724, 901, 1518
PleI	GAGTC	3 cut(s)	134, 1024, 1093
PpsI	GAGTC	3 cut(s)	134, 1024, 1093
PshAI	GACNNNNGTC	1 cut(s)	38
Psp1406I	AACGTT	1 cut(s)	1173
Psp6I	CCWGG	3 cut(s)	170, 469, 1472
PspGI	CCWGG	3 cut(s)	170, 469, 1472
PspN4I	GGNNCC	3 cut(s)	632, 676, 1377
PspXI	VCTCGAGB	1 cut(s)	1091
PstNI	CAGNNNCTG	2 cut(s)	254, 1479
PsyI	GACNNNGTC	1 cut(s)	479
PvuII	CAGCTG	1 cut(s)	818
RsaI	GTAC	2 cut(s)	68, 297
RsaNI	GTAC	2 cut(s)	67, 296
SaqAI	TTAA	5 cut(s)	273, 503, 585, 714, 1283
SatI	GCNGC	7 cut(s)	17, 93, 162, 264, 723, 900, 1517
Sau3AI	GATC	5 cut(s)	6, 139, 187, 364, 1441
SchI	GAGTC	3 cut(s)	135, 1024, 1094
ScrFI	CCNGG	3 cut(s)	172, 471, 1474
SduI	GDGCHC	1 cut(s)	808
SexAI	ACCWGGT	1 cut(s)	170
SfaNI	GCATC	1 cut(s)	934
Sfr274I	CTCGAG	1 cut(s)	1091
SgrAI	CRCCGGYG	1 cut(s)	88
SlaI	CTCGAG	1 cut(s)	1091
SmlI	CTYRAG	3 cut(s)	760, 1091, 1247
SmoI	CTYRAG	3 cut(s)	760, 1091, 1247
SsiI	CCGC	6 cut(s)	16, 31, 92, 95, 162, 603
SspMI	CTAG	3 cut(s)	12, 572, 768
StyD4I	CCNGG	3 cut(s)	170, 469, 1472
StyI	CCWWGG	1 cut(s)	1131
TaaI	ACNGT	6 cut(s)	47, 300, 850, 1218, 1426, 1468
TaiI	ACGT	3 cut(s)	531, 887, 1176
TaqI	TCGA	4 cut(s)	124, 420, 516, 1092
TauI	GCSGC	3 cut(s)	19, 95, 164
TfiI	GAWTC	7 cut(s)	521, 575, 735, 821, 864, 989, 1507
Tru1I	TTAA	5 cut(s)	273, 503, 585, 714, 1283
Tru9I	TTAA	5 cut(s)	273, 503, 585, 714, 1283
TscAI	CASTG	2 cut(s)	255, 1044
TseFI	GTSAC	4 cut(s)	404, 473, 567, 1086
TseI	GCWGC	4 cut(s)	263, 722, 899, 1516
Tsp45I	GTSAC	4 cut(s)	404, 473, 567, 1086
TspDTI	ATGAA	4 cut(s)	678, 908, 1079, 1095
TspGWI	ACGGA	5 cut(s)	54, 72, 108, 1047, 1410
TspRI	CASTG	2 cut(s)	255, 1044
Tth111I	GACNNNGTC	1 cut(s)	479
VneI	GTGCAC	1 cut(s)	804
XapI	RAATTY	5 cut(s)	377, 495, 550, 964, 1365
XhoI	CTCGAG	1 cut(s)	1091
XmiI	GTMKAC	1 cut(s)	614
XspI	CTAG	3 cut(s)	12, 572, 768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.