pycom16g14280

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008027
Physical Location & Seq
Forward (+)
10319838 .. 10321802
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGTCAAGGTTGGATATATTATCATCTTCTTTTCAGAGTAGTATATCGAACTTAAGTTGCAGTCCATCTACACCTGATTCTGATTCTTACTTAATGAAATCCACGAGTCCTTCCAGAAATGATAGTTTTCTTAGTGGCATGGAAGTGCAAGTGGCAGGTGGAGCTGCTGGTGAAGACAGGGTCCAAGCTGTTTGTTCTGAAGAAAATGGGTGGCTCTTTTGTGCAATTTATGACGGTTTCAATGGAAGAGACGCAGCTGATTTTCTGGCTGGGACACTGTATGAAACCATCATATTTCACTTTAATTCATTAGACTGGGAATCAAGTCAGGAATCCATCAAAGCTTCTAATGATCTAGATATAGATGAGTCCCTTCCATATATAGGAGATGATAGTACTATTAGTGGAGAAAGATTTTCATCCGGAATTTATGCACACAAGGATTCAAGTTTTGACAATACCTCTAAGAATGCTCCATGTGCAAAAGTAAAAACATTAACTGATTCGTTTAGGCATGGGGTGCTTGATAGCCTCCAACAAGCTCTTAGTCAGGCCGAGAATGATTTTCTATGCATGGTTGACCAGGAAATGGTGGATCGCCCAGATTTAGTTTCCGTTGGGTGTTGTGTTTTAGTTGCACTTCTACATGGAAAGGATTTGTATACACTTAATTTAGGTGACAGCCGAGCTGTATTGGCAACATATGGTGATGGTTATTTAATGAGTGGGAGTGATAGGTTAAAGGCTATCCAGCTCACTGATAGTCATACAGTTGATGATGAAAGTGAGAGAATGCGAGTTTTGTGTGAACATCCTGACGATCCCATGACCGTTGTGGCTGGAAAAGTAAAGGGGAAATTGAAGGTCACTCGAGCTTTTGGAGTTGGTTACTTGAAAAAGAAAAAACTGAATGATGCATTGATGGGAATTCTTCAAGTTCGTAACCTCATAAGCCCACCATATGTTTCCACTCAACCAGCGTTGAATGTGCACAGAATATCAAAATCTGATTGCTTTGTAGTACTTGGTAGTGATGGCCTGTTTGACTTTTTTACCAATGACGAGGCAGTAAAGCTTGTCCACTCCTATATCTTGAGCAACCCCTCTGGCGATCCAGCTAAGTTTCTGCTCGAGCAGCTTGTAGTAAGAGCAGCCAATTCTGCAGGTTTCAGCATGGAAGAACTCATGAACATTCCAGCAGGAAGGCGGAGGAAGTATCACGATGATGTAACTGTAATTGTGATTATGCTCGGTACAGATCAGCGCACGACAAAGGCGTCAACGTGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

431

Amino Acids

46.96

Weight (kDa)

4.9

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 165 - 368 8.4e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
AasI GACNNNNNNGTC 1 cut(s) 1276
Acc36I ACCTGC 2 cut(s) 146, 1154
AccB7I CCANNNNNTGG 1 cut(s) 589
AccI GTMKAC 1 cut(s) 662
AccIII TCCGGA 1 cut(s) 422
AciI CCGC 1 cut(s) 1207
AclWI GGATC 3 cut(s) 603, 815, 1106
AcsI RAATTY 2 cut(s) 426, 927
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 1277
AfaI GTAC 3 cut(s) 397, 1023, 1255
AfiI CCNNNNNNNGG 2 cut(s) 383, 589
AflII CTTAAG 1 cut(s) 52
AgsI TTSAA 6 cut(s) 241, 447, 862, 895, 935, 985
AjnI CCWGG 1 cut(s) 582
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 1 cut(s) 243
Alw44I GTGCAC 1 cut(s) 989
AlwI GGATC 3 cut(s) 603, 815, 1106
Ama87I CYCGRG 2 cut(s) 870, 1130
Aor13HI TCCGGA 1 cut(s) 422
AoxI GGCC 2 cut(s) 552, 1036
ApaLI GTGCAC 1 cut(s) 989
ApeKI GCWGC 4 cut(s) 164, 254, 1135, 1151
ApoI RAATTY 2 cut(s) 426, 927
AspLEI GCGC 1 cut(s) 1266
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 3 cut(s) 182, 689, 719
AvaI CYCGRG 2 cut(s) 870, 1130
AvaII GGWCC 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 993
BauI CACGAG 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 993
BbvI GCAGC 4 cut(s) 151, 266, 1147, 1163
BccI CCATC 6 cut(s) 73, 296, 344, 704, 916, 1028
BciT130I CCWGG 1 cut(s) 584
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 1161
BfrI CTTAAG 1 cut(s) 52
BfuAI ACCTGC 2 cut(s) 146, 1154
BisI GCNGC 4 cut(s) 165, 255, 1136, 1152
BlsI GCNGC 4 cut(s) 166, 256, 1137, 1153
BmcAI AGTACT 2 cut(s) 397, 1023
Bme1390I CCNGG 1 cut(s) 584
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 2 cut(s) 870, 1130
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 584
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 1114
BsaBI GATNNNNATC 1 cut(s) 418
BsaHI GRCGYC 1 cut(s) 1277
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 589
Bse1I ACTGG 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 418
BseAI TCCGGA 1 cut(s) 422
BseBI CCWGG 1 cut(s) 584
BseGI GGATG 2 cut(s) 419, 811
BseJI GATNNNNATC 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 383, 589
BseNI ACTGG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 993
BseXI GCAGC 4 cut(s) 151, 266, 1147, 1163
BseYI CCCAGC 1 cut(s) 269
BshFI GGCC 2 cut(s) 554, 1038
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 2 cut(s) 870, 1130
BsiSI CCGG 1 cut(s) 423
BslFI GGGAC 2 cut(s) 286, 355
BslI CCNNNNNNNGG 2 cut(s) 383, 589
BsmAI GTCTC 1 cut(s) 243
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 2 cut(s) 286, 355
BsmI GAATGC 2 cut(s) 475, 798
BsnI GGCC 2 cut(s) 554, 1038
BsoBI CYCGRG 2 cut(s) 870, 1130
Bsp1286I GDGCHC 1 cut(s) 993
Bsp13I TCCGGA 1 cut(s) 422
Bsp143I GATC 5 cut(s) 352, 595, 820, 1111, 1258
BspACI CCGC 1 cut(s) 1207
BspANI GGCC 2 cut(s) 554, 1038
BspEI TCCGGA 1 cut(s) 422
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 1 cut(s) 182
BspMAI CTGCAG 1 cut(s) 1165
BspMI ACCTGC 2 cut(s) 146, 1154
BspPI GGATC 3 cut(s) 603, 815, 1106
BspTI CTTAAG 1 cut(s) 52
BsrI ACTGG 1 cut(s) 320
BssMI GATC 5 cut(s) 352, 595, 820, 1111, 1258
BssNAI GTATAC 1 cut(s) 663
BssNI GRCGYC 1 cut(s) 1277
BssSI CACGAG 1 cut(s) 103
Bst1107I GTATAC 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 5 cut(s) 236, 279, 772, 832, 1234
Bst6I CTCTTC 1 cut(s) 241
BstACI GRCGYC 1 cut(s) 1277
BstAFI CTTAAG 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 520
BstDEI CTNAG 4 cut(s) 131, 465, 545, 1119
BstF5I GGATG 2 cut(s) 419, 811
BstHHI GCGC 1 cut(s) 1266
BstKTI GATC 5 cut(s) 355, 598, 823, 1114, 1261
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 5 cut(s) 352, 595, 820, 1111, 1258
BstMWI GCNNNNNNNGC 6 cut(s) 161, 479, 520, 695, 1135, 1160
BstNI CCWGG 1 cut(s) 584
BstSCI CCNGG 1 cut(s) 582
BstSFI CTRYAG 1 cut(s) 1161
BstSLI GKGCMC 1 cut(s) 993
BstV1I GCAGC 4 cut(s) 151, 266, 1147, 1163
BstV2I GAAGAC 1 cut(s) 180
BstZ17I GTATAC 1 cut(s) 663
BsuRI GGCC 2 cut(s) 554, 1038
BtsCI GGATG 2 cut(s) 419, 811
BtsIMutI CAGTG 2 cut(s) 275, 756
BveI ACCTGC 2 cut(s) 146, 1154
CciI TCATGA 1 cut(s) 1185
CfoI GCGC 1 cut(s) 1266
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 2 cut(s) 260, 1266
Csp6I GTAC 3 cut(s) 396, 1022, 1254
CviAII CATG 8 cut(s) 139, 477, 515, 574, 647, 826, 1174, 1186
CviQI GTAC 3 cut(s) 396, 1022, 1254
DdeI CTNAG 4 cut(s) 131, 465, 545, 1119
DpnI GATC 5 cut(s) 354, 597, 822, 1113, 1260
DpnII GATC 5 cut(s) 352, 595, 820, 1111, 1258
DrdI GACNNNNNNGTC 1 cut(s) 1276
DseDI GACNNNNNNGTC 1 cut(s) 1276
Eam1104I CTCTTC 1 cut(s) 241
EarI CTCTTC 1 cut(s) 241
EciI GGCGGA 1 cut(s) 1222
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 2 cut(s) 870, 1130
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 1 cut(s) 582
EcoT22I ATGCAT 2 cut(s) 575, 919
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 8 cut(s) 142, 480, 518, 577, 650, 829, 1177, 1189
FaqI GGGAC 2 cut(s) 286, 355
FatI CATG 8 cut(s) 138, 476, 514, 573, 646, 825, 1173, 1185
FauNDI CATATG 2 cut(s) 703, 961
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 4 cut(s) 165, 255, 1136, 1152
FokI GGATG 2 cut(s) 406, 798
Fsp4HI GCNGC 4 cut(s) 165, 255, 1136, 1152
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 1265
GluI GCNGC 4 cut(s) 165, 255, 1136, 1152
GsaI CCCAGC 1 cut(s) 273
HaeIII GGCC 2 cut(s) 554, 1038
HapII CCGG 1 cut(s) 423
HgaI GACGC 2 cut(s) 260, 1266
HhaI GCGC 1 cut(s) 1266
Hin1I GRCGYC 1 cut(s) 1277
Hin1II CATG 8 cut(s) 142, 480, 518, 577, 650, 829, 1177, 1189
Hin6I GCGC 1 cut(s) 1264
HinP1I GCGC 1 cut(s) 1264
HincII GTYRAC 2 cut(s) 580, 1281
HindII GTYRAC 2 cut(s) 580, 1281
HindIII AAGCTT 2 cut(s) 342, 1073
HinfI GANTC 8 cut(s) 77, 83, 106, 320, 332, 368, 443, 503
HpaII CCGG 1 cut(s) 423
HphI GGTGA 3 cut(s) 182, 689, 719
Hpy166II GTNNAC 6 cut(s) 580, 663, 809, 991, 1081, 1281
Hpy188I TCNGA 4 cut(s) 36, 82, 199, 1009
Hpy188III TCNNGA 8 cut(s) 114, 329, 356, 423, 815, 1093, 1186, 1220
Hpy8I GTNNAC 6 cut(s) 580, 663, 809, 991, 1081, 1281
HpyAV CCTTC 4 cut(s) 120, 383, 856, 1197
HpyCH4III ACNGT 5 cut(s) 236, 279, 772, 832, 1234
HpyCH4IV ACGT 1 cut(s) 1283
HpyF10VI GCNNNNNNNGC 6 cut(s) 161, 479, 520, 695, 1135, 1160
HpyF3I CTNAG 4 cut(s) 131, 465, 545, 1119
HpySE526I ACGT 1 cut(s) 1283
Hsp92I GRCGYC 1 cut(s) 1277
Hsp92II CATG 8 cut(s) 142, 480, 518, 577, 650, 829, 1177, 1189
HspAI GCGC 1 cut(s) 1264
Kpn2I TCCGGA 1 cut(s) 422
Kzo9I GATC 5 cut(s) 352, 595, 820, 1111, 1258
LmnI GCTCC 2 cut(s) 161, 478
Lsp1109I GCAGC 4 cut(s) 151, 266, 1147, 1163
LweI GCATC 1 cut(s) 904
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 1283
MaeIII GTNAC 5 cut(s) 677, 865, 887, 941, 1228
MalI GATC 5 cut(s) 354, 597, 822, 1113, 1260
MboI GATC 5 cut(s) 352, 595, 820, 1111, 1258
MboII GAAGA 6 cut(s) 18, 185, 212, 258, 923, 1190
MhlI GDGCHC 1 cut(s) 993
MluCI AATT 8 cut(s) 225, 304, 426, 670, 857, 927, 1156, 1236
MlyI GAGTC 2 cut(s) 115, 377
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 6 cut(s) 472, 542, 956, 1057, 1114, 1203
Mph1103I ATGCAT 2 cut(s) 575, 919
MroI TCCGGA 1 cut(s) 422
MseI TTAA 7 cut(s) 53, 92, 303, 497, 669, 719, 740
MslI CAYNNNNRTG 2 cut(s) 143, 1224
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 52
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 1 cut(s) 584
Mva1269I GAATGC 2 cut(s) 475, 798
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 6 cut(s) 161, 479, 520, 695, 1135, 1160
NdeI CATATG 2 cut(s) 703, 961
NdeII GATC 5 cut(s) 352, 595, 820, 1111, 1258
NlaIII CATG 8 cut(s) 142, 480, 518, 577, 650, 829, 1177, 1189
NlaIV GGNNCC 1 cut(s) 182
NmeAIII GCCGAG 2 cut(s) 580, 710
NmuCI GTSAC 2 cut(s) 677, 865
NsiI ATGCAT 2 cut(s) 575, 919
PaeR7I CTCGAG 2 cut(s) 870, 1130
PagI TCATGA 1 cut(s) 1185
PaqCI CACCTGC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 1274
PctI GAATGC 2 cut(s) 475, 798
PfeI GAWTC 6 cut(s) 77, 83, 320, 332, 443, 503
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 4 cut(s) 166, 256, 1137, 1153
PleI GAGTC 2 cut(s) 114, 376
PpsI GAGTC 2 cut(s) 114, 376
Psp6I CCWGG 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 1 cut(s) 181
PspXI VCTCGAGB 2 cut(s) 870, 1130
PstI CTGCAG 1 cut(s) 1165
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 397, 1023, 1255
RsaNI GTAC 3 cut(s) 396, 1022, 1254
RseI CAYNNNNRTG 2 cut(s) 143, 1224
SaqAI TTAA 7 cut(s) 53, 92, 303, 497, 669, 719, 740
SatI GCNGC 4 cut(s) 165, 255, 1136, 1152
Sau3AI GATC 5 cut(s) 352, 595, 820, 1111, 1258
Sau96I GGNCC 1 cut(s) 181
ScaI AGTACT 2 cut(s) 397, 1023
SchI GAGTC 2 cut(s) 115, 377
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 1 cut(s) 904
SfcI CTRYAG 1 cut(s) 1161
Sfr274I CTCGAG 2 cut(s) 870, 1130
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 2 cut(s) 870, 1130
SmiMI CAYNNNNRTG 2 cut(s) 143, 1224
SmlI CTYRAG 4 cut(s) 52, 870, 1093, 1130
SmoI CTYRAG 4 cut(s) 52, 870, 1093, 1130
Sse9I AATT 8 cut(s) 225, 304, 426, 670, 857, 927, 1156, 1236
SsiI CCGC 1 cut(s) 1207
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 1 cut(s) 582
TaaI ACNGT 5 cut(s) 236, 279, 772, 832, 1234
TaiI ACGT 1 cut(s) 1286
TaqI TCGA 3 cut(s) 47, 871, 1131
TasI AATT 8 cut(s) 225, 304, 426, 670, 857, 927, 1156, 1236
TatI WGTACW 2 cut(s) 395, 1021
TfiI GAWTC 6 cut(s) 77, 83, 320, 332, 443, 503
Tru1I TTAA 7 cut(s) 53, 92, 303, 497, 669, 719, 740
Tru9I TTAA 7 cut(s) 53, 92, 303, 497, 669, 719, 740
TscAI CASTG 2 cut(s) 282, 763
TseFI GTSAC 2 cut(s) 677, 865
TseI GCWGC 4 cut(s) 164, 254, 1135, 1151
Tsp45I GTSAC 2 cut(s) 677, 865
TspDTI ATGAA 6 cut(s) 110, 297, 297, 408, 795, 1202
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 2 cut(s) 282, 763
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 1 cut(s) 589
Vha464I CTTAAG 1 cut(s) 52
VneI GTGCAC 1 cut(s) 989
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 2 cut(s) 426, 927
XbaI TCTAGA 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XhoI CTCGAG 2 cut(s) 870, 1130
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 1 cut(s) 356
ZrmI AGTACT 2 cut(s) 397, 1023
Zsp2I ATGCAT 2 cut(s) 575, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.